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The map label for this gene is proB

Identifier: 119899460

GI number: 119899460

Start: 3478164

End: 3479279

Strand: Direct

Name: proB

Synonym: azo3170

Alternate gene names: 119899460

Gene position: 3478164-3479279 (Clockwise)

Preceding gene: 119899459

Following gene: 119899461

Centisome position: 79.48

GC content: 68.73

Gene sequence:

>1116_bases
ATGAGAGACAAGATCAAGAATGCGCGCCGGCTTATCGTGAAAGTCGGCAGCGCCCTCGTGACCAACAACGGCGCGGGGCT
GGATCCTGCCGCACTCGACGACTGGGCGCGACAGATCGCCGCGCTGCGTGCGCGGGGCCGCGAGATCGTGCTGGTGTCCT
CGGGCGCGATCGCCGCCGGCATGCAGCGCCTGGGGTGGGTCAAGCGGCCGCACGAGATGCACCGGCTGCAGGCCGCCGCC
GCCGTGGGTCAGATGGGGCTTGTGGAGGCTTATGAAAAGGCGTTCTCGCGCCACGGCCTGCAGACTGCTCAGATCCTGCT
CACCCACGAGGACCTCGCCGACCGCACGCGCTATCTGAATGCGCGCTCCACGCTCGTCACGCTGCTGGAACTGGGCGTCG
TCCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAGATCAAGTTCGGCGACAACGACACCCTCGGCGCCCTGGTC
GCGAATCTGGTGGAAGCCGACACGCTGATCATCCTCACCGACCAGCGCGGCCTGTACACCGCCGACCCGAGGCGTGACCC
GGGTGCGACGCTGATCAGCGAGGGACGCGCTGAGGACAGGCAGTACGAAGCCATGGCGGGCGGCGCTGGCAGCGGCATCA
GCAAGGGCGGCATGATCACCAAGATCCGCGCGGCGCAGCGTGCCGCCCGCAGCGGCGCGCACACCTGCATTGCCAGCGGG
CGGGAAACCGACCCGCTGCTGCGTCTGGCCGACGGTGAAGCGGTGGGCACGCTGCTCTATGCCGACAGCACGCCGCTGCA
GGCCCGCAAGCAGTGGCTGGCCGACCACCTGCAACTCGCGGGCAGCCTGATCGTCGATGCCGGCGCCGCGCGCGCCCTGG
ATGACGGCCGCAGCCTGCTGCCGGTCGGCGTCGTCGAGGTGCAGGGCGAGTTCAAACGCGGGGCCGCGGTCGCCTGTCGC
GACGAACACGGCACCGAACTCGCGCGCGGCCTGGTCAATTACAGCAGCGCGGAATGCCGTCGCATCCTGCGCCGCCCGAC
CAGCGACATCGAGCAACTGCTCGGCTACATCGACGAGCCCGAACTCATCCATCGCGACAACCTGGTCATCCGCTAG

Upstream 100 bases:

>100_bases
CTGGCCGAGGACGCGCTGGCCGAGGACGCGCTGGACGACGACGCCGACGGCGAGGACGCCGACCCGAACGCCCGCTGAAC
ACCTGCGCCGAATCGACATC

Downstream 100 bases:

>100_bases
GCGGCCCAGGCACGCGGCGCCCGTCCGACGCCCTTCCTTCTGCTGCTGGCAGTCGGCAATTGCCCTGCTGCCGACATGCC
ATCTGGCATGCCGGCGCAGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRDKIKNARRLIVKVGSALVTNNGAGLDPAALDDWARQIAALRARGREIVLVSSGAIAAGMQRLGWVKRPHEMHRLQAAA
AVGQMGLVEAYEKAFSRHGLQTAQILLTHEDLADRTRYLNARSTLVTLLELGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLVEADTLIILTDQRGLYTADPRRDPGATLISEGRAEDRQYEAMAGGAGSGISKGGMITKIRAAQRAARSGAHTCIASG
RETDPLLRLADGEAVGTLLYADSTPLQARKQWLADHLQLAGSLIVDAGAARALDDGRSLLPVGVVEVQGEFKRGAAVACR
DEHGTELARGLVNYSSAECRRILRRPTSDIEQLLGYIDEPELIHRDNLVIR

Sequences:

>Translated_371_residues
MRDKIKNARRLIVKVGSALVTNNGAGLDPAALDDWARQIAALRARGREIVLVSSGAIAAGMQRLGWVKRPHEMHRLQAAA
AVGQMGLVEAYEKAFSRHGLQTAQILLTHEDLADRTRYLNARSTLVTLLELGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLVEADTLIILTDQRGLYTADPRRDPGATLISEGRAEDRQYEAMAGGAGSGISKGGMITKIRAAQRAARSGAHTCIASG
RETDPLLRLADGEAVGTLLYADSTPLQARKQWLADHLQLAGSLIVDAGAARALDDGRSLLPVGVVEVQGEFKRGAAVACR
DEHGTELARGLVNYSSAECRRILRRPTSDIEQLLGYIDEPELIHRDNLVIR
>Mature_371_residues
MRDKIKNARRLIVKVGSALVTNNGAGLDPAALDDWARQIAALRARGREIVLVSSGAIAAGMQRLGWVKRPHEMHRLQAAA
AVGQMGLVEAYEKAFSRHGLQTAQILLTHEDLADRTRYLNARSTLVTLLELGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLVEADTLIILTDQRGLYTADPRRDPGATLISEGRAEDRQYEAMAGGAGSGISKGGMITKIRAAQRAARSGAHTCIASG
RETDPLLRLADGEAVGTLLYADSTPLQARKQWLADHLQLAGSLIVDAGAARALDDGRSLLPVGVVEVQGEFKRGAAVACR
DEHGTELARGLVNYSSAECRRILRRPTSDIEQLLGYIDEPELIHRDNLVIR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=306, Percent_Identity=33.9869281045752, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI62912457, Length=304, Percent_Identity=34.2105263157895, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=43.75, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=33.5766423357664, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=33.5766423357664, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=34.6987951807229, Blast_Score=198, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=32.7751196172249, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=33.7931034482759, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_AZOSB (A1KAD1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934673.1
- ProteinModelPortal:   A1KAD1
- SMR:   A1KAD1
- STRING:   A1KAD1
- GeneID:   4607827
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3170
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A1KAD1
- ProtClustDB:   PRK05429
- BioCyc:   ASP62928:AZO3170-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39996; Mature: 39996

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKIKNARRLIVKVGSALVTNNGAGLDPAALDDWARQIAALRARGREIVLVSSGAIAAG
CCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWVKRPHEMHRLQAAAAVGQMGLVEAYEKAFSRHGLQTAQILLTHEDLADRTRYLN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
ARSTLVTLLELGVVPIINENDTVVTDEIKFGDNDTLGALVANLVEADTLIILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRRDPGATLISEGRAEDRQYEAMAGGAGSGISKGGMITKIRAAQRAARSGAHTCIASG
CCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHCCC
RETDPLLRLADGEAVGTLLYADSTPLQARKQWLADHLQLAGSLIVDAGAARALDDGRSLL
CCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
PVGVVEVQGEFKRGAAVACRDEHGTELARGLVNYSSAECRRILRRPTSDIEQLLGYIDEP
EEEEEEECCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCC
ELIHRDNLVIR
CEEECCCCEEC
>Mature Secondary Structure
MRDKIKNARRLIVKVGSALVTNNGAGLDPAALDDWARQIAALRARGREIVLVSSGAIAAG
CCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWVKRPHEMHRLQAAAAVGQMGLVEAYEKAFSRHGLQTAQILLTHEDLADRTRYLN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
ARSTLVTLLELGVVPIINENDTVVTDEIKFGDNDTLGALVANLVEADTLIILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRRDPGATLISEGRAEDRQYEAMAGGAGSGISKGGMITKIRAAQRAARSGAHTCIASG
CCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHCCC
RETDPLLRLADGEAVGTLLYADSTPLQARKQWLADHLQLAGSLIVDAGAARALDDGRSLL
CCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
PVGVVEVQGEFKRGAAVACRDEHGTELARGLVNYSSAECRRILRRPTSDIEQLLGYIDEP
EEEEEEECCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCC
ELIHRDNLVIR
CEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA