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The map label for this gene is panB

Identifier: 119899434

GI number: 119899434

Start: 3451731

End: 3452546

Strand: Direct

Name: panB

Synonym: azo3144

Alternate gene names: 119899434

Gene position: 3451731-3452546 (Clockwise)

Preceding gene: 119899433

Following gene: 119899435

Centisome position: 78.88

GC content: 67.16

Gene sequence:

>816_bases
ATGAGCTATCTTCAGGACGACAAGCCCGTCACGCTCTTCGAACTCGGCAAGATGCGCGCCGAAGGCCGCAAGATCGCCAT
GCTCACCTGTTACGACGCGAGCTTCGCCGCGCTGCTCGAACGCAATGGCGTCGACGTGCTGCTGGTGGGCGATTCGCTCG
GCAACGTGTTGCAGGGCCAGAAATCCACGCTGCCGGTCACGCTGGAGCAGATGGCCTATCACACCGAATGCGTGGTGCGC
GGCAGCTCGCGCCCCTTCGTCGTCACCGACATGCCCTTCGGCAGCTACCAGGAAAGCCCCGAGCAGGCGATGCGCAACGC
CGCCCGGCTGATGGCGGCCGGTGCCGAGATGGTCAAGCTCGAAGGCGGTGCCTTCATGGCCGAGACCGTGCGCTTCCTGG
TCGAGCGCGGCGTGCCGGTCTGCGCCCACATCGGGCTCACGCCGCAATCGGTGCATCAACTCGGCGGCTATCGCGTGCAG
GGCCGCAGTGAAGAAGGCGCAGCCCGGCTGAAGGCCGACGCGCTGGCGCTGGAACAGGCCGGCGCCGCGCTGGTCGTGCT
CGAGATGGTGCCCGCGGCAGTCGCCAGCGACATCACCCGCAGCCTGTCCACGATGGCAACGATCGGCATCGGCGCCGGTG
CCGACTGCAGCGGCCAGGTGCTGGTGCTGCACGACATGATCGGCGTCTATCCCGGCAAGAAGGCCCGCTTCGTCAAGAAT
TTCATGGAAGGTGCCGCCAGCATCGACGCGGCGGTCGCGGCCTATGTCGCCGCCGTGAAGGACGGCAGCTTCCCCGCCGC
CGAACACTGCTACTGA

Upstream 100 bases:

>100_bases
CGGCTACGCCGGCTGATCCCTCCTCCGCCTCCCGCCCGCCGCGCCCACTTGTGCGCCGCATCAGTTTGCGGAAAACTCCC
GCCTCCCTACGGAGTCTGCC

Downstream 100 bases:

>100_bases
TCCCGCGGACGCCGCCATGCAAATCCACACCACCGTCGCCAGCCTGCGTGCAGCCCGCGCCCGCGCCGGCCGCGTCGCCC
TCGTCCCCACCATGGGCAAC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSYLQDDKPVTLFELGKMRAEGRKIAMLTCYDASFAALLERNGVDVLLVGDSLGNVLQGQKSTLPVTLEQMAYHTECVVR
GSSRPFVVTDMPFGSYQESPEQAMRNAARLMAAGAEMVKLEGGAFMAETVRFLVERGVPVCAHIGLTPQSVHQLGGYRVQ
GRSEEGAARLKADALALEQAGAALVVLEMVPAAVASDITRSLSTMATIGIGAGADCSGQVLVLHDMIGVYPGKKARFVKN
FMEGAASIDAAVAAYVAAVKDGSFPAAEHCY

Sequences:

>Translated_271_residues
MSYLQDDKPVTLFELGKMRAEGRKIAMLTCYDASFAALLERNGVDVLLVGDSLGNVLQGQKSTLPVTLEQMAYHTECVVR
GSSRPFVVTDMPFGSYQESPEQAMRNAARLMAAGAEMVKLEGGAFMAETVRFLVERGVPVCAHIGLTPQSVHQLGGYRVQ
GRSEEGAARLKADALALEQAGAALVVLEMVPAAVASDITRSLSTMATIGIGAGADCSGQVLVLHDMIGVYPGKKARFVKN
FMEGAASIDAAVAAYVAAVKDGSFPAAEHCY
>Mature_270_residues
SYLQDDKPVTLFELGKMRAEGRKIAMLTCYDASFAALLERNGVDVLLVGDSLGNVLQGQKSTLPVTLEQMAYHTECVVRG
SSRPFVVTDMPFGSYQESPEQAMRNAARLMAAGAEMVKLEGGAFMAETVRFLVERGVPVCAHIGLTPQSVHQLGGYRVQG
RSEEGAARLKADALALEQAGAALVVLEMVPAAVASDITRSLSTMATIGIGAGADCSGQVLVLHDMIGVYPGKKARFVKNF
MEGAASIDAAVAAYVAAVKDGSFPAAEHCY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=50.7575757575758, Blast_Score=262, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319653, Length=252, Percent_Identity=38.0952380952381, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_AZOSB (A1KAA5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934647.1
- ProteinModelPortal:   A1KAA5
- SMR:   A1KAA5
- STRING:   A1KAA5
- GeneID:   4607817
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3144
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   MACRISA
- PhylomeDB:   A1KAA5
- ProtClustDB:   PRK00311
- BioCyc:   ASP62928:AZO3144-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28665; Mature: 28534

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: ACT_SITE 188-188 BINDING 90-90 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLQDDKPVTLFELGKMRAEGRKIAMLTCYDASFAALLERNGVDVLLVGDSLGNVLQGQ
CCCCCCCCCEEHHHHHHHHCCCCEEEEEEEECHHHHHHHHCCCCEEEEECCCHHHHHCCC
KSTLPVTLEQMAYHTECVVRGSSRPFVVTDMPFGSYQESPEQAMRNAARLMAAGAEMVKL
CCCCCEEHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
EGGAFMAETVRFLVERGVPVCAHIGLTPQSVHQLGGYRVQGRSEEGAARLKADALALEQA
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHC
GAALVVLEMVPAAVASDITRSLSTMATIGIGAGADCSGQVLVLHDMIGVYPGKKARFVKN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHH
FMEGAASIDAAVAAYVAAVKDGSFPAAEHCY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SYLQDDKPVTLFELGKMRAEGRKIAMLTCYDASFAALLERNGVDVLLVGDSLGNVLQGQ
CCCCCCCCEEHHHHHHHHCCCCEEEEEEEECHHHHHHHHCCCCEEEEECCCHHHHHCCC
KSTLPVTLEQMAYHTECVVRGSSRPFVVTDMPFGSYQESPEQAMRNAARLMAAGAEMVKL
CCCCCEEHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
EGGAFMAETVRFLVERGVPVCAHIGLTPQSVHQLGGYRVQGRSEEGAARLKADALALEQA
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHC
GAALVVLEMVPAAVASDITRSLSTMATIGIGAGADCSGQVLVLHDMIGVYPGKKARFVKN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHH
FMEGAASIDAAVAAYVAAVKDGSFPAAEHCY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA