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The map label for this gene is gacA [H]

Identifier: 119899270

GI number: 119899270

Start: 3273206

End: 3273850

Strand: Direct

Name: gacA [H]

Synonym: azo2980

Alternate gene names: 119899270

Gene position: 3273206-3273850 (Clockwise)

Preceding gene: 119899269

Following gene: 119899271

Centisome position: 74.8

GC content: 70.39

Gene sequence:

>645_bases
ATGACCTCCCTCGCCGACCTCCGCATCCTGCTCGCCGACGACCATGCCGTCGTCCGCATGGGTTTCCGCCTGCTGATCGA
GGGCGCCGGTGCCGTCGTCGTCGGCGAGGCCGACAGCGGCGAAGCGGCGATCACCGCGTATGCCGAACATCAGCCCGACG
CGCTGGTGATGGACGTTTCCATGCCCGGCATCGGCGGCCTTGCCGCGCTCGAACGCCTGCGCGCGCGCTACCCGCATGCG
CGGGTGCTGATGCTCTCTGCCCACGACGACACCCAGATCCCGGCGCGCGCGCTGCAGGCCGGCGCCACCGGCTACCTCTC
CAAGCGCGCCCATCCCAGCGAACTGCTGCGTGCGATCGGCGAAGTCGCCCACGGCCGCCGCTACCTTGACCCCGAAATCG
CCCCGCGCCTGGCCCTGGCGCGGCTGGGCGGCAGCGAGCACCCGGCGGACGCGCTGACCGGCAAGGAATTCAACGTCTTC
CTGCAACTCGCCCGCGGCCGCTCGGTGGCCGAGGTGGCGGAAACGCTGAACCTGAGCACCAGCACGGTGGGCACCCACCT
GTACCACATCAAGCAGAAGCTCAACGCGGGCAACGCCGCGGAACTGGCCTTGATTGCGGTACGGGCGGGGTTGGTGGAGG
CTTGA

Upstream 100 bases:

>100_bases
GCTGCTAACCCCATCCCCACCCCCACCCTCCCCTTGAAGGGGAGGGAGTGAACACCCCCCCAGCACCACATCGCGTTCCC
CTCTCCACCGGAATTCCACC

Downstream 100 bases:

>100_bases
GGGACGGGGGTGAAACACGGTTGGCATAACGTCAGCGTCAAACCAATCGCTCCCGTTCCAACGGTCAAGACAGGAACTTT
CAAACACGGCTACCTCCCGT

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSLADLRILLADDHAVVRMGFRLLIEGAGAVVVGEADSGEAAITAYAEHQPDALVMDVSMPGIGGLAALERLRARYPHA
RVLMLSAHDDTQIPARALQAGATGYLSKRAHPSELLRAIGEVAHGRRYLDPEIAPRLALARLGGSEHPADALTGKEFNVF
LQLARGRSVAEVAETLNLSTSTVGTHLYHIKQKLNAGNAAELALIAVRAGLVEA

Sequences:

>Translated_214_residues
MTSLADLRILLADDHAVVRMGFRLLIEGAGAVVVGEADSGEAAITAYAEHQPDALVMDVSMPGIGGLAALERLRARYPHA
RVLMLSAHDDTQIPARALQAGATGYLSKRAHPSELLRAIGEVAHGRRYLDPEIAPRLALARLGGSEHPADALTGKEFNVF
LQLARGRSVAEVAETLNLSTSTVGTHLYHIKQKLNAGNAAELALIAVRAGLVEA
>Mature_213_residues
TSLADLRILLADDHAVVRMGFRLLIEGAGAVVVGEADSGEAAITAYAEHQPDALVMDVSMPGIGGLAALERLRARYPHAR
VLMLSAHDDTQIPARALQAGATGYLSKRAHPSELLRAIGEVAHGRRYLDPEIAPRLALARLGGSEHPADALTGKEFNVFL
QLARGRSVAEVAETLNLSTSTVGTHLYHIKQKLNAGNAAELALIAVRAGLVEA

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=39.8104265402844, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=39.6984924623116, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788521, Length=191, Percent_Identity=33.5078534031414, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=34.4262295081967, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786747, Length=195, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788712, Length=203, Percent_Identity=26.6009852216749, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788546, Length=199, Percent_Identity=26.6331658291457, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=29.6875, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22632; Mature: 22501

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLADLRILLADDHAVVRMGFRLLIEGAGAVVVGEADSGEAAITAYAEHQPDALVMDVS
CCCCHHEEEEEECCHHHHHHHHHHEEECCCEEEEECCCCCCEEEEEEECCCCCEEEEEEC
MPGIGGLAALERLRARYPHARVLMLSAHDDTQIPARALQAGATGYLSKRAHPSELLRAIG
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHHHHHH
EVAHGRRYLDPEIAPRLALARLGGSEHPADALTGKEFNVFLQLARGRSVAEVAETLNLST
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHEEEEEECCCCCHHHHHHHHCCCH
STVGTHLYHIKQKLNAGNAAELALIAVRAGLVEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSLADLRILLADDHAVVRMGFRLLIEGAGAVVVGEADSGEAAITAYAEHQPDALVMDVS
CCCHHEEEEEECCHHHHHHHHHHEEECCCEEEEECCCCCCEEEEEEECCCCCEEEEEEC
MPGIGGLAALERLRARYPHARVLMLSAHDDTQIPARALQAGATGYLSKRAHPSELLRAIG
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHHHHHH
EVAHGRRYLDPEIAPRLALARLGGSEHPADALTGKEFNVFLQLARGRSVAEVAETLNLST
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHEEEEEECCCCCHHHHHHHHCCCH
STVGTHLYHIKQKLNAGNAAELALIAVRAGLVEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]