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The map label for this gene is liaS [H]

Identifier: 119899261

GI number: 119899261

Start: 3260287

End: 3261633

Strand: Reverse

Name: liaS [H]

Synonym: azo2971

Alternate gene names: 119899261

Gene position: 3261633-3260287 (Counterclockwise)

Preceding gene: 119899265

Following gene: 119899260

Centisome position: 74.53

GC content: 73.94

Gene sequence:

>1347_bases
ATGGAGGCTGGCGTGGATCTGCGGGTGCGCTTGGTCGTGCGGCTGTCGGGCTTTGCCCTGGCGCTGCTGGCGGGAGCCTG
CCTGGTGGTGGGGCTCAATCTCAGGCAGGACGTGGCGGAGGAGATGGCGGCCTCGGCGCGTCTGGCCGACCTGATGCTGG
CGATCGGGCAGACGCAGCCGGCGGAATCGATCGCCGGACTGATCGCCGCGGGCGAACTGCGCCATGTGCAGGTGACGCTC
GATCGTTCAGTTTTGGAGCAATCGCCGGCGCAGCCCCCTGTGCCGGCGCCGACCAGCGGTGCCGACGCGGTGCTGCTGGC
GCTGTTCGGCTGGCTGCTGCCCTCGCAGGCGGACGCCGCCGTGCGCCGGATCGAGCTGGCCGGCGGCGACGGCGCGGCGC
TGCTGATCCGCGCCGAACCCTTGTCCGAACTGCGCGAGATCCTGCGCGATGCCGCCCGCCTGCTCGGCCTGCTGCTGCTG
TTTGCCGCCGGCACCGTGCTGGCCGCCTGGCACGCCGCCGACCGCGCGCTGCGCCCGGTGCGCGCGCTGGAGGACAGCCT
CGCCCGGCTTGCCCGCGGTGACACGCAGACCGCGCTGCCGTCCTTCCAGCTCAAGGAGTTCCGCCGCATCGCCGGTGCCA
TCGAACGGCTGGCCGACAGCCTCGCGCAGTCGCGCGCGGCAGAACGCCGGCTCGGCCGCTGCCTGATGGAGGTGCAGGAA
ACCGAGCGGCGCGAACTCGCGCGCGAATTGCACGACGAGTTCGGCCAGTCGCTCACCGCCATCGGCGCCGCCGCCGCCTT
TGTCGAACGCCATGCCGCCGCCGCCGAGCCGGCGCAGCTCGCCGAGTGCGCGCGCGACATCCGCGGCGAATCGGGGCGCA
TGTCGGCCCAGGTTCGCGGCCTGCTGCGCCAGCTGCGGCCGCACGGCCTGGAGGGGCTGGGCATGCTCGATGCGCTGCGC
GAACTCATCGCCGGCTGGCACCAGCGCCTGCCCGGCGTCGCGCTGGAGGTGGCGCTGCCGGCGCGGCTGCCGGCGCTGTC
GGCGGAAGCCGGCCTCGCGCTCTACCGTACCGTGCAGGAGGCGCTCACCAACGTGCTGCGCCATGCCGCCGCCAGCCGGG
TGGACATCGCGCTCGCCGCCGATGGCAATGGACTGCTGCTAAGGGTGGCCGACGACGGCTGCGGCCGCGCCGCCGAGGTG
ATGCGGCGCGCCGGCGGCGGTCTGCTCGGCATGCGCGAACGCGCCGCAATGGCGGGCGGCGAGTTCCGCCTGGAAGACCG
CCCCGGCGGCGGATTGGTGACCCTGCTGCGCCTGACTACGATGAACGGGAAGGAGGCCCCGCGATGA

Upstream 100 bases:

>100_bases
CGGCGGTCGCGGGAGCGCGGAACCGCAAAACCGGGAAAATTTCCCGATGCGGGTAATGGCACGTGCCTTGCGTAATGAAG
GGCAAACAAGACCGAAAACG

Downstream 100 bases:

>100_bases
TCCGCATCCTGCTGCTCGACGACCATGCCGTGGTGCGTACCGGCTACCGCCGCCTGATCGACGCCGAGCCCGGCCTGCAG
GTGGTGGCCGAGGCCGCCAC

Product: putative two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MEAGVDLRVRLVVRLSGFALALLAGACLVVGLNLRQDVAEEMAASARLADLMLAIGQTQPAESIAGLIAAGELRHVQVTL
DRSVLEQSPAQPPVPAPTSGADAVLLALFGWLLPSQADAAVRRIELAGGDGAALLIRAEPLSELREILRDAARLLGLLLL
FAAGTVLAAWHAADRALRPVRALEDSLARLARGDTQTALPSFQLKEFRRIAGAIERLADSLAQSRAAERRLGRCLMEVQE
TERRELARELHDEFGQSLTAIGAAAAFVERHAAAAEPAQLAECARDIRGESGRMSAQVRGLLRQLRPHGLEGLGMLDALR
ELIAGWHQRLPGVALEVALPARLPALSAEAGLALYRTVQEALTNVLRHAAASRVDIALAADGNGLLLRVADDGCGRAAEV
MRRAGGGLLGMRERAAMAGGEFRLEDRPGGGLVTLLRLTTMNGKEAPR

Sequences:

>Translated_448_residues
MEAGVDLRVRLVVRLSGFALALLAGACLVVGLNLRQDVAEEMAASARLADLMLAIGQTQPAESIAGLIAAGELRHVQVTL
DRSVLEQSPAQPPVPAPTSGADAVLLALFGWLLPSQADAAVRRIELAGGDGAALLIRAEPLSELREILRDAARLLGLLLL
FAAGTVLAAWHAADRALRPVRALEDSLARLARGDTQTALPSFQLKEFRRIAGAIERLADSLAQSRAAERRLGRCLMEVQE
TERRELARELHDEFGQSLTAIGAAAAFVERHAAAAEPAQLAECARDIRGESGRMSAQVRGLLRQLRPHGLEGLGMLDALR
ELIAGWHQRLPGVALEVALPARLPALSAEAGLALYRTVQEALTNVLRHAAASRVDIALAADGNGLLLRVADDGCGRAAEV
MRRAGGGLLGMRERAAMAGGEFRLEDRPGGGLVTLLRLTTMNGKEAPR
>Mature_448_residues
MEAGVDLRVRLVVRLSGFALALLAGACLVVGLNLRQDVAEEMAASARLADLMLAIGQTQPAESIAGLIAAGELRHVQVTL
DRSVLEQSPAQPPVPAPTSGADAVLLALFGWLLPSQADAAVRRIELAGGDGAALLIRAEPLSELREILRDAARLLGLLLL
FAAGTVLAAWHAADRALRPVRALEDSLARLARGDTQTALPSFQLKEFRRIAGAIERLADSLAQSRAAERRLGRCLMEVQE
TERRELARELHDEFGQSLTAIGAAAAFVERHAAAAEPAQLAECARDIRGESGRMSAQVRGLLRQLRPHGLEGLGMLDALR
ELIAGWHQRLPGVALEVALPARLPALSAEAGLALYRTVQEALTNVLRHAAASRVDIALAADGNGLLLRVADDGCGRAAEV
MRRAGGGLLGMRERAAMAGGEFRLEDRPGGGLVTLLRLTTMNGKEAPR

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=219, Percent_Identity=32.4200913242009, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082311, Length=213, Percent_Identity=35.2112676056338, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788812, Length=209, Percent_Identity=28.2296650717703, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47499; Mature: 47499

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAGVDLRVRLVVRLSGFALALLAGACLVVGLNLRQDVAEEMAASARLADLMLAIGQTQP
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AESIAGLIAAGELRHVQVTLDRSVLEQSPAQPPVPAPTSGADAVLLALFGWLLPSQADAA
HHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
VRRIELAGGDGAALLIRAEPLSELREILRDAARLLGLLLLFAAGTVLAAWHAADRALRPV
HHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALEDSLARLARGDTQTALPSFQLKEFRRIAGAIERLADSLAQSRAAERRLGRCLMEVQE
HHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TERRELARELHDEFGQSLTAIGAAAAFVERHAAAAEPAQLAECARDIRGESGRMSAQVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
LLRQLRPHGLEGLGMLDALRELIAGWHQRLPGVALEVALPARLPALSAEAGLALYRTVQE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
ALTNVLRHAAASRVDIALAADGNGLLLRVADDGCGRAAEVMRRAGGGLLGMRERAAMAGG
HHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCC
EFRLEDRPGGGLVTLLRLTTMNGKEAPR
CEEECCCCCCCCEEEHHHHCCCCCCCCC
>Mature Secondary Structure
MEAGVDLRVRLVVRLSGFALALLAGACLVVGLNLRQDVAEEMAASARLADLMLAIGQTQP
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AESIAGLIAAGELRHVQVTLDRSVLEQSPAQPPVPAPTSGADAVLLALFGWLLPSQADAA
HHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
VRRIELAGGDGAALLIRAEPLSELREILRDAARLLGLLLLFAAGTVLAAWHAADRALRPV
HHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALEDSLARLARGDTQTALPSFQLKEFRRIAGAIERLADSLAQSRAAERRLGRCLMEVQE
HHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TERRELARELHDEFGQSLTAIGAAAAFVERHAAAAEPAQLAECARDIRGESGRMSAQVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
LLRQLRPHGLEGLGMLDALRELIAGWHQRLPGVALEVALPARLPALSAEAGLALYRTVQE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
ALTNVLRHAAASRVDIALAADGNGLLLRVADDGCGRAAEVMRRAGGGLLGMRERAAMAGG
HHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCC
EFRLEDRPGGGLVTLLRLTTMNGKEAPR
CEEECCCCCCCCEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]