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The map label for this gene is bioB

Identifier: 119899107

GI number: 119899107

Start: 3096650

End: 3097630

Strand: Direct

Name: bioB

Synonym: azo2817

Alternate gene names: 119899107

Gene position: 3096650-3097630 (Clockwise)

Preceding gene: 119899104

Following gene: 119899108

Centisome position: 70.76

GC content: 67.28

Gene sequence:

>981_bases
ATGACAACGACCTTGACCGCCCCCACCCCCGCCGCCGCGGAGGCGCCCCGCAAGAAATGGACGGTGGCCGAGGTGCAGGC
GCTGTTCGACCTGCCCTTCCTCGACTTGGTGTTCCAGGCCCAGCAGGTTCACCGCGCGCACTTCGCCGCCAACGAGGTGC
AGCGCTCCACGTTGCTGTCGATCAAGACGGGTGGCTGCTCGGAAGACTGCGGCTACTGTTCGCAGTCGGCACGCTACGAC
ACCGGGCTGGAACGCGAACGTCTGCTGCCGCTGGACGAGGTGCTCGAGCACGCGCGCGCGGCCAAGGCCAAGGGCTCGTC
CCGCTTCTGCATGGGTGCCGCGTGGCGTGGTCCCAAGGACAAGGACATGGCGCCGGTGCTGGAGATGGTGCGCGAAGTCA
AGAAGCTGGGGCTGGAAACCTGCGTCACGCTCGGCATGTTGAAGGAGGGACAGGCCGAGCAGCTCGCGGAAGCCGGACTG
GACTATTACAACCACAACATCGACACCGCGCCCGAGTTCTACGGCCAGATCGTCACCACCCATACGCTGCAGGACCGCCT
CGACACGCTCGACAAGGTGCGCGATGCCGGCATCAACGTGTGCTGCGGCGGCATCGTCGGGATGGGTGAAACCCAGACCA
GCCGCGCCGGCCTGATCGCCCAGCTGGCCAACATGACCCCGCCGCCCGACTCGGTGCCGATCAACAACCTGGTCGCGATC
CCCGGCACGCCGCTCGCCGACGGCGGCACGATCGACGGCTTCGACTTCGTCCGCACCATCGCCGCGGCCCGCATCACCAT
GCCCACCAGCTACGTGCGGCTCTCCGCCGGGCGCCAGCAGATGAGCGACGAGATGCAGGCGCTGTGCTTCATGGCCGGCG
CCAACTCGATCTTCTACGGCGAACGCCTGCTGACCACCGACAATCCGGAATCCGACCGCGACGACGTGCTGTTCGCACGC
CTCGGCCTGCGTTCGGTGTGA

Upstream 100 bases:

>100_bases
CGAAATCCAGCAGCCGCAACAAGGTCTTGGGTTGATTTGACAAGCTTTCGAGCGATGAATGAACATGTCGGGCTTCGCAT
TAGAGTCCGGAAACCGAGTT

Downstream 100 bases:

>100_bases
TGGCCGCGGCCGACACCGACTTCCACCTCGACCGCGCCCTGCTGCGGCGGCGCTTCGCGGCCGCAGCAGGGCGCTACGAC
GAGGCCGACGTCCTGGCGCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTTTLTAPTPAAAEAPRKKWTVAEVQALFDLPFLDLVFQAQQVHRAHFAANEVQRSTLLSIKTGGCSEDCGYCSQSARYD
TGLERERLLPLDEVLEHARAAKAKGSSRFCMGAAWRGPKDKDMAPVLEMVREVKKLGLETCVTLGMLKEGQAEQLAEAGL
DYYNHNIDTAPEFYGQIVTTHTLQDRLDTLDKVRDAGINVCCGGIVGMGETQTSRAGLIAQLANMTPPPDSVPINNLVAI
PGTPLADGGTIDGFDFVRTIAAARITMPTSYVRLSAGRQQMSDEMQALCFMAGANSIFYGERLLTTDNPESDRDDVLFAR
LGLRSV

Sequences:

>Translated_326_residues
MTTTLTAPTPAAAEAPRKKWTVAEVQALFDLPFLDLVFQAQQVHRAHFAANEVQRSTLLSIKTGGCSEDCGYCSQSARYD
TGLERERLLPLDEVLEHARAAKAKGSSRFCMGAAWRGPKDKDMAPVLEMVREVKKLGLETCVTLGMLKEGQAEQLAEAGL
DYYNHNIDTAPEFYGQIVTTHTLQDRLDTLDKVRDAGINVCCGGIVGMGETQTSRAGLIAQLANMTPPPDSVPINNLVAI
PGTPLADGGTIDGFDFVRTIAAARITMPTSYVRLSAGRQQMSDEMQALCFMAGANSIFYGERLLTTDNPESDRDDVLFAR
LGLRSV
>Mature_325_residues
TTTLTAPTPAAAEAPRKKWTVAEVQALFDLPFLDLVFQAQQVHRAHFAANEVQRSTLLSIKTGGCSEDCGYCSQSARYDT
GLERERLLPLDEVLEHARAAKAKGSSRFCMGAAWRGPKDKDMAPVLEMVREVKKLGLETCVTLGMLKEGQAEQLAEAGLD
YYNHNIDTAPEFYGQIVTTHTLQDRLDTLDKVRDAGINVCCGGIVGMGETQTSRAGLIAQLANMTPPPDSVPINNLVAIP
GTPLADGGTIDGFDFVRTIAAARITMPTSYVRLSAGRQQMSDEMQALCFMAGANSIFYGERLLTTDNPESDRDDVLFARL
GLRSV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=66.3430420711974, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=47.3354231974922, Blast_Score=318, Evalue=8e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_AZOSB (A1K9C8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934320.1
- ProteinModelPortal:   A1K9C8
- SMR:   A1K9C8
- STRING:   A1K9C8
- GeneID:   4608511
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2817
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1K9C8
- ProtClustDB:   CLSK2298551
- BioCyc:   ASP62928:AZO2817-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35407; Mature: 35276

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTLTAPTPAAAEAPRKKWTVAEVQALFDLPFLDLVFQAQQVHRAHFAANEVQRSTLLS
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IKTGGCSEDCGYCSQSARYDTGLERERLLPLDEVLEHARAAKAKGSSRFCMGAAWRGPKD
EECCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
KDMAPVLEMVREVKKLGLETCVTLGMLKEGQAEQLAEAGLDYYNHNIDTAPEFYGQIVTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HTLQDRLDTLDKVRDAGINVCCGGIVGMGETQTSRAGLIAQLANMTPPPDSVPINNLVAI
HHHHHHHHHHHHHHHCCCCEEECCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEC
PGTPLADGGTIDGFDFVRTIAAARITMPTSYVRLSAGRQQMSDEMQALCFMAGANSIFYG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
ERLLTTDNPESDRDDVLFARLGLRSV
CEEECCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTTLTAPTPAAAEAPRKKWTVAEVQALFDLPFLDLVFQAQQVHRAHFAANEVQRSTLLS
CCCCCCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IKTGGCSEDCGYCSQSARYDTGLERERLLPLDEVLEHARAAKAKGSSRFCMGAAWRGPKD
EECCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
KDMAPVLEMVREVKKLGLETCVTLGMLKEGQAEQLAEAGLDYYNHNIDTAPEFYGQIVTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HTLQDRLDTLDKVRDAGINVCCGGIVGMGETQTSRAGLIAQLANMTPPPDSVPINNLVAI
HHHHHHHHHHHHHHHCCCCEEECCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEC
PGTPLADGGTIDGFDFVRTIAAARITMPTSYVRLSAGRQQMSDEMQALCFMAGANSIFYG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
ERLLTTDNPESDRDDVLFARLGLRSV
CEEECCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA