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The map label for this gene is fleR [H]

Identifier: 119899004

GI number: 119899004

Start: 2997698

End: 2999176

Strand: Reverse

Name: fleR [H]

Synonym: azo2714

Alternate gene names: 119899004

Gene position: 2999176-2997698 (Counterclockwise)

Preceding gene: 119899005

Following gene: 119899003

Centisome position: 68.54

GC content: 67.88

Gene sequence:

>1479_bases
ATGATCGAACACCTGAACATCCTCGTCGTGGAGGACGATGCCGCGCTGCGCGACGCGGTGTGCCTGACCCTGGAAATGGC
GGGGCACGGCGTGACCGGCGTGGATGGCGGGCCGAGCGCGCTCGCAGAACTCGAACGGCAGTCCTTCAACTTGGTGGTGT
CAGACCTGCGCATGCAGCCGATGGACGGCCTGCAACTGCTCGGCGAGATCCGCGCCCGCCTGCCGCAGTTGCCGGTGCTG
CTGATGACCGCCTACGGCGATGTCGACAAGGCGGTGGCGGCGATGCGCGGCGGGGCCTGCGACTTCCTGATGAAGCCGTT
CGAGCCCGAGGTGCTGCTGGAAAACGTGCGGCGCTACGCCGCGGTGCAGCCGAGCGCCGAGGACACGATCGCCGAGGACC
CGCACACCCGCAACCTGCTGGCGCTGGCGGCGCGCGTGGCCGAAACCGACGCCACCGTGCTGCTCACCGGCGAATCCGGC
ACCGGCAAAGAGGTCTTCGCGCGCTACATCCATCACCATTCGCTGCGCCGCAAGGGGCCCTTCGTCGCGATCAACTGCGC
GGCGATTCCGGAGAACCTGCTCGAAGCGACCCTGTTCGGCTACGAGAAGGGCGCCTTCACCGGCGCGCAGACCGCGCAGC
CTGGCAAGTTCGAGCAGGCGCAGGACGGCACCATCCTGCTCGACGAAATCTCCGAGATGCCGCTTGGCCTGCAGGCCAAG
ATGCTGCGCGTGCTGCAGGAACGCGAGGTCGAGCGCGTGGGCGGCAAGAAGCCGGTCAGTCTCGATATCCGCGTGCTCGC
CACCAGCAACCGCGACATGGTCAAGGAGGTCGCCGCCGGGCGCTTCCGCGAGGACTTGTACTACCGCCTCAACGTCTTCC
CGCTTGCGATTCCGTCGCTGCGCGAACGCCCGGGCGACATCCTGCCGCTGGCGCGCCATTTCCTCGGCCGCCACGGCGAC
CGCCTGAAGCGCCACGCCCGTTTCTCGCCCGAGGCGGAATTCCTGCTGGCGCGCTACCACTGGCCGGGCAATGTGCGCGA
ACTCGAAAACACCATGCATCGCGCGCTGATCCTCACCGTGGGCGACACCGTCAGCGCCGAAACCGTCCGCCTGTGCCTGC
CGCACTGGACGGGCGAGGCAATGGCAAGCCCCATTCCGCAGCCCGTGGCAACGCCGGTCCCGGCCGCCGCATGGACACCG
CCACCCGCAGCGCCAGCGGCAAATCCTGCCGCCACTTTTGCCGCTTCCAGCGTGGCAGCGGCAAACATTGCCGGCGGTTT
CGCGCTGCCGCCGGTTCAGGCAGCCGACGCGGGTGGCGCGCGCCCGTCCAACATGAAGGACCTGGAGCGCGAACACATTC
TCGGCACGCTGCGCGAGGTGGGCGGCTCGCGCAAGAAAGCCGTTGAAAAGCTTGGCATTTCCGAACGCACGCTGCGCTAC
AAGCTGCAGCAGTATCGTGAAGAAGGCTACGACGTCTGA

Upstream 100 bases:

>100_bases
CCCTGAGTTCCGCCCCGGGCCAGGGTTCCACCTTCACCCTCAACCTGCCCTTGTGGAGCGCCGCCCCGGCAGACGCGGCG
GACAGCGGAGCGCACGCCTC

Downstream 100 bases:

>100_bases
ACCCGCGCACCGCGCGGCCCGCTTCTTGCATTGGTTCTGGTAAAGCCGCATTGCGGCCCGAGGTCCGGCAAAACCGTGTG
TCACGTGGTGGCACGTGGTT

Product: sigma-54 dependent response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIEHLNILVVEDDAALRDAVCLTLEMAGHGVTGVDGGPSALAELERQSFNLVVSDLRMQPMDGLQLLGEIRARLPQLPVL
LMTAYGDVDKAVAAMRGGACDFLMKPFEPEVLLENVRRYAAVQPSAEDTIAEDPHTRNLLALAARVAETDATVLLTGESG
TGKEVFARYIHHHSLRRKGPFVAINCAAIPENLLEATLFGYEKGAFTGAQTAQPGKFEQAQDGTILLDEISEMPLGLQAK
MLRVLQEREVERVGGKKPVSLDIRVLATSNRDMVKEVAAGRFREDLYYRLNVFPLAIPSLRERPGDILPLARHFLGRHGD
RLKRHARFSPEAEFLLARYHWPGNVRELENTMHRALILTVGDTVSAETVRLCLPHWTGEAMASPIPQPVATPVPAAAWTP
PPAAPAANPAATFAASSVAAANIAGGFALPPVQAADAGGARPSNMKDLEREHILGTLREVGGSRKKAVEKLGISERTLRY
KLQQYREEGYDV

Sequences:

>Translated_492_residues
MIEHLNILVVEDDAALRDAVCLTLEMAGHGVTGVDGGPSALAELERQSFNLVVSDLRMQPMDGLQLLGEIRARLPQLPVL
LMTAYGDVDKAVAAMRGGACDFLMKPFEPEVLLENVRRYAAVQPSAEDTIAEDPHTRNLLALAARVAETDATVLLTGESG
TGKEVFARYIHHHSLRRKGPFVAINCAAIPENLLEATLFGYEKGAFTGAQTAQPGKFEQAQDGTILLDEISEMPLGLQAK
MLRVLQEREVERVGGKKPVSLDIRVLATSNRDMVKEVAAGRFREDLYYRLNVFPLAIPSLRERPGDILPLARHFLGRHGD
RLKRHARFSPEAEFLLARYHWPGNVRELENTMHRALILTVGDTVSAETVRLCLPHWTGEAMASPIPQPVATPVPAAAWTP
PPAAPAANPAATFAASSVAAANIAGGFALPPVQAADAGGARPSNMKDLEREHILGTLREVGGSRKKAVEKLGISERTLRY
KLQQYREEGYDV
>Mature_492_residues
MIEHLNILVVEDDAALRDAVCLTLEMAGHGVTGVDGGPSALAELERQSFNLVVSDLRMQPMDGLQLLGEIRARLPQLPVL
LMTAYGDVDKAVAAMRGGACDFLMKPFEPEVLLENVRRYAAVQPSAEDTIAEDPHTRNLLALAARVAETDATVLLTGESG
TGKEVFARYIHHHSLRRKGPFVAINCAAIPENLLEATLFGYEKGAFTGAQTAQPGKFEQAQDGTILLDEISEMPLGLQAK
MLRVLQEREVERVGGKKPVSLDIRVLATSNRDMVKEVAAGRFREDLYYRLNVFPLAIPSLRERPGDILPLARHFLGRHGD
RLKRHARFSPEAEFLLARYHWPGNVRELENTMHRALILTVGDTVSAETVRLCLPHWTGEAMASPIPQPVATPVPAAAWTP
PPAAPAANPAATFAASSVAAANIAGGFALPPVQAADAGGARPSNMKDLEREHILGTLREVGGSRKKAVEKLGISERTLRY
KLQQYREEGYDV

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=505, Percent_Identity=38.6138613861386, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1790437, Length=494, Percent_Identity=38.2591093117409, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1790299, Length=491, Percent_Identity=38.4928716904277, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1788905, Length=388, Percent_Identity=42.7835051546392, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI87082117, Length=239, Percent_Identity=51.8828451882845, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=51.5151515151515, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=251, Percent_Identity=48.605577689243, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=365, Percent_Identity=37.5342465753425, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786524, Length=279, Percent_Identity=43.7275985663082, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081872, Length=220, Percent_Identity=43.1818181818182, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1787583, Length=228, Percent_Identity=42.1052631578947, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.4330708661417, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081858, Length=221, Percent_Identity=31.2217194570136, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790552, Length=113, Percent_Identity=36.283185840708, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=34.1880341880342, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=33.6283185840708, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786599, Length=144, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53590; Mature: 53590

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEHLNILVVEDDAALRDAVCLTLEMAGHGVTGVDGGPSALAELERQSFNLVVSDLRMQP
CCCCEEEEEEECCHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
MDGLQLLGEIRARLPQLPVLLMTAYGDVDKAVAAMRGGACDFLMKPFEPEVLLENVRRYA
CHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHH
AVQPSAEDTIAEDPHTRNLLALAARVAETDATVLLTGESGTGKEVFARYIHHHSLRRKGP
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
FVAINCAAIPENLLEATLFGYEKGAFTGAQTAQPGKFEQAQDGTILLDEISEMPLGLQAK
EEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHH
MLRVLQEREVERVGGKKPVSLDIRVLATSNRDMVKEVAAGRFREDLYYRLNVFPLAIPSL
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCHH
RERPGDILPLARHFLGRHGDRLKRHARFSPEAEFLLARYHWPGNVRELENTMHRALILTV
HCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHEEEEEE
GDTVSAETVRLCLPHWTGEAMASPIPQPVATPVPAAAWTPPPAAPAANPAATFAASSVAA
CCCCCCCHHEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ANIAGGFALPPVQAADAGGARPSNMKDLEREHILGTLREVGGSRKKAVEKLGISERTLRY
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
KLQQYREEGYDV
HHHHHHHHCCCC
>Mature Secondary Structure
MIEHLNILVVEDDAALRDAVCLTLEMAGHGVTGVDGGPSALAELERQSFNLVVSDLRMQP
CCCCEEEEEEECCHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
MDGLQLLGEIRARLPQLPVLLMTAYGDVDKAVAAMRGGACDFLMKPFEPEVLLENVRRYA
CHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHH
AVQPSAEDTIAEDPHTRNLLALAARVAETDATVLLTGESGTGKEVFARYIHHHSLRRKGP
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
FVAINCAAIPENLLEATLFGYEKGAFTGAQTAQPGKFEQAQDGTILLDEISEMPLGLQAK
EEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHH
MLRVLQEREVERVGGKKPVSLDIRVLATSNRDMVKEVAAGRFREDLYYRLNVFPLAIPSL
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCHH
RERPGDILPLARHFLGRHGDRLKRHARFSPEAEFLLARYHWPGNVRELENTMHRALILTV
HCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHEEEEEE
GDTVSAETVRLCLPHWTGEAMASPIPQPVATPVPAAAWTPPPAAPAANPAATFAASSVAA
CCCCCCCHHEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ANIAGGFALPPVQAADAGGARPSNMKDLEREHILGTLREVGGSRKKAVEKLGISERTLRY
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
KLQQYREEGYDV
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]