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The map label for this gene is fleS [H]

Identifier: 119899005

GI number: 119899005

Start: 2999173

End: 3000354

Strand: Reverse

Name: fleS [H]

Synonym: azo2715

Alternate gene names: 119899005

Gene position: 3000354-2999173 (Counterclockwise)

Preceding gene: 119899019

Following gene: 119899004

Centisome position: 68.56

GC content: 71.83

Gene sequence:

>1182_bases
ATGCCACCCCAGATGCCGCCGATCACCCCCGCCGATGCCTCGCCCGCCGCGCCGCTGGATGCCGCGCAGCTGGCCGAGGC
TTTTGCGCTGTTCAGCCGTGCATCGGAAGAACTCTCCACCGCCTACAACGCGCTGCAGGGCCAGGTGGTGCAGCTCACGG
AGCGCCTGACGGTGCTGATGGGCGCGCTGCCCGCCGGCGTGGTAGTGGTGGATCGGGCCGGCAGCGTGGTGCAGGTGAAC
CGCGCCGCCGAATCCCTGCTGGGGGCCGGCCTCGCGGGGCAACGGTGGCCGACGATCGCACGCGCGCTGCAGGGCACCGG
CACCCCCGGCGAGTTCACGCTGGAACATGCCGACGGCCAGCGCCGGCTGTCGGTCTCGGAAACCGCGCTGGAGTCGGGCG
AGGAGCGCATCCTGCTGCTGCACGACGTGACCGAAACCCACCGCATGCGGCTGGCGGCCGAGCGCAACGAACGCCTCGCG
GCGATGGGCGAAATGGTGGCGGGGCTGGCTCACCAGCTGCGCACGCCGCTTGCCGCCGCGCTGCTCTACACCGGCAATCT
GCGTCAGCCGGAACTCGGCGCGCCCGAGCGCGCCAAGGTGGCCGACCGCGCGATCGAACGCCTGCGCTATCTCGAACGCC
TGATCCGCGACATGCTGCTGTTCGCGCGTGGCGACAGCCTCGGCCGCCAGCATTTCGGCGTGTGCGACCTCGTTGCCGAA
CTCACCCACACCCTCGAACCGCTGGCACGGGCGCGCCAGATCGAATTTTCCAGCGCCTGCAGCTGCGGCGAGCTGAGCCT
GTACGGCGACCGCAAGGCGCTCGGCGGCGCGCTCACCAACCTGCTGGAAAACGCCATCCAGGCCACCGAGGCCGGCGGCA
CGGTGAGCTGCAGCGCGAGTTGCGCGCAGGGCGCCGACGGCGAGCAGGTGCGCTTCGTCGTCAGCGACAGCGGACGCGGC
ATCCCGGCCGAACTGCAGGAGCGCCTGTTCGAACCCTTCTTCACCACCCGCGCCGAAGGCACCGGCCTCGGCCTCGCCAT
CGCGCGCGGCGTCGCACGCGGCCACGGCGGCGACATCTCCCTGAGTTCCGCCCCGGGCCAGGGTTCCACCTTCACCCTCA
ACCTGCCCTTGTGGAGCGCCGCCCCGGCAGACGCGGCGGACAGCGGAGCGCACGCCTCATGA

Upstream 100 bases:

>100_bases
CGCGGTCGCACGAAAAGCGGCATTTTTTGCCGCCTGTTTGCCGGCTAACATTCAAGGGCGTGCAGCTAAAGTGCCAGCCG
TGAAACTGGAGCCAGCGCCG

Downstream 100 bases:

>100_bases
TCGAACACCTGAACATCCTCGTCGTGGAGGACGATGCCGCGCTGCGCGACGCGGTGTGCCTGACCCTGGAAATGGCGGGG
CACGGCGTGACCGGCGTGGA

Product: putative sensory box sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPPQMPPITPADASPAAPLDAAQLAEAFALFSRASEELSTAYNALQGQVVQLTERLTVLMGALPAGVVVVDRAGSVVQVN
RAAESLLGAGLAGQRWPTIARALQGTGTPGEFTLEHADGQRRLSVSETALESGEERILLLHDVTETHRMRLAAERNERLA
AMGEMVAGLAHQLRTPLAAALLYTGNLRQPELGAPERAKVADRAIERLRYLERLIRDMLLFARGDSLGRQHFGVCDLVAE
LTHTLEPLARARQIEFSSACSCGELSLYGDRKALGGALTNLLENAIQATEAGGTVSCSASCAQGADGEQVRFVVSDSGRG
IPAELQERLFEPFFTTRAEGTGLGLAIARGVARGHGGDISLSSAPGQGSTFTLNLPLWSAAPADAADSGAHAS

Sequences:

>Translated_393_residues
MPPQMPPITPADASPAAPLDAAQLAEAFALFSRASEELSTAYNALQGQVVQLTERLTVLMGALPAGVVVVDRAGSVVQVN
RAAESLLGAGLAGQRWPTIARALQGTGTPGEFTLEHADGQRRLSVSETALESGEERILLLHDVTETHRMRLAAERNERLA
AMGEMVAGLAHQLRTPLAAALLYTGNLRQPELGAPERAKVADRAIERLRYLERLIRDMLLFARGDSLGRQHFGVCDLVAE
LTHTLEPLARARQIEFSSACSCGELSLYGDRKALGGALTNLLENAIQATEAGGTVSCSASCAQGADGEQVRFVVSDSGRG
IPAELQERLFEPFFTTRAEGTGLGLAIARGVARGHGGDISLSSAPGQGSTFTLNLPLWSAAPADAADSGAHAS
>Mature_392_residues
PPQMPPITPADASPAAPLDAAQLAEAFALFSRASEELSTAYNALQGQVVQLTERLTVLMGALPAGVVVVDRAGSVVQVNR
AAESLLGAGLAGQRWPTIARALQGTGTPGEFTLEHADGQRRLSVSETALESGEERILLLHDVTETHRMRLAAERNERLAA
MGEMVAGLAHQLRTPLAAALLYTGNLRQPELGAPERAKVADRAIERLRYLERLIRDMLLFARGDSLGRQHFGVCDLVAEL
THTLEPLARARQIEFSSACSCGELSLYGDRKALGGALTNLLENAIQATEAGGTVSCSASCAQGADGEQVRFVVSDSGRGI
PAELQERLFEPFFTTRAEGTGLGLAIARGVARGHGGDISLSSAPGQGSTFTLNLPLWSAAPADAADSGAHAS

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=336, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=36.6379310344828, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=33.1983805668016, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788713, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=242, Percent_Identity=30.5785123966942, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=247, Percent_Identity=27.9352226720648, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081816, Length=269, Percent_Identity=32.7137546468402, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790300, Length=350, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=339, Percent_Identity=25.6637168141593, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=246, Percent_Identity=29.2682926829268, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787894, Length=243, Percent_Identity=28.8065843621399, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=246, Percent_Identity=26.0162601626016, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=28.75, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=346, Percent_Identity=26.5895953757225, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=28.8702928870293, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41317; Mature: 41186

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPQMPPITPADASPAAPLDAAQLAEAFALFSRASEELSTAYNALQGQVVQLTERLTVLM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALPAGVVVVDRAGSVVQVNRAAESLLGAGLAGQRWPTIARALQGTGTPGEFTLEHADGQ
HHCCCCEEEEECCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCC
RRLSVSETALESGEERILLLHDVTETHRMRLAAERNERLAAMGEMVAGLAHQLRTPLAAA
CEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLYTGNLRQPELGAPERAKVADRAIERLRYLERLIRDMLLFARGDSLGRQHFGVCDLVAE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTHTLEPLARARQIEFSSACSCGELSLYGDRKALGGALTNLLENAIQATEAGGTVSCSAS
HHHHHHHHHHHHHCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCH
CAQGADGEQVRFVVSDSGRGIPAELQERLFEPFFTTRAEGTGLGLAIARGVARGHGGDIS
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEE
LSSAPGQGSTFTLNLPLWSAAPADAADSGAHAS
ECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PPQMPPITPADASPAAPLDAAQLAEAFALFSRASEELSTAYNALQGQVVQLTERLTVLM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALPAGVVVVDRAGSVVQVNRAAESLLGAGLAGQRWPTIARALQGTGTPGEFTLEHADGQ
HHCCCCEEEEECCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCC
RRLSVSETALESGEERILLLHDVTETHRMRLAAERNERLAAMGEMVAGLAHQLRTPLAAA
CEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLYTGNLRQPELGAPERAKVADRAIERLRYLERLIRDMLLFARGDSLGRQHFGVCDLVAE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTHTLEPLARARQIEFSSACSCGELSLYGDRKALGGALTNLLENAIQATEAGGTVSCSAS
HHHHHHHHHHHHHCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCH
CAQGADGEQVRFVVSDSGRGIPAELQERLFEPFFTTRAEGTGLGLAIARGVARGHGGDIS
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEE
LSSAPGQGSTFTLNLPLWSAAPADAADSGAHAS
ECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]