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The map label for this gene is dosC [H]

Identifier: 119898981

GI number: 119898981

Start: 2965210

End: 2967042

Strand: Direct

Name: dosC [H]

Synonym: azo2691

Alternate gene names: 119898981

Gene position: 2965210-2967042 (Clockwise)

Preceding gene: 119898980

Following gene: 119898982

Centisome position: 67.76

GC content: 69.78

Gene sequence:

>1833_bases
ATGACGCGCACTCCCCCTTCCGCCTTTCCCCCCTCCCGACCGGGAGGAGACGGCCGTAGACCCGGCACCACCGCCGAAAC
CGGTACCGCCCCCGAAATTGCCCGCGAAGCCCTGCGACAGCTCGCTGCCCAGCGCGTACCGCCGACGCCGGACAACTACC
GCGCCTTCTACCATCAGATTGCCGGCACCGCGCCCGAAGAGGTTTTTCCCACCCGCGCCCTGAAGGCGATCGCCGGCGCG
CTGCCCCGCTCCACGCCCGAGGCCGTGCGCTTGGCGCGCCAGTTCGAAGAAGCAGTCGCCGCCGGTCAGTGGCCGGCGCT
CAAGCTTGCCATCGTTGCCATCTGCGAGACGCGCGGCGAGGCGCAACTCGCCTGGGGTTTGCTGATCCGCGACCTCGTCG
GCCAGCTCGAACGCAACCAGCAGGGCTTCACCCGCGCGCGCAAGCTCGAATCGCTGCAGCACGTCCTCGCCGGTTCCAGC
GCGGACCCCGCCCAGCTCCACATGCGTCTGCAGGGCCTGCTGCGCAGCTGGGGCCAGGCCGCGGAAGGCGAGGAAGAGGC
CCTGCCGGGCGGCCCAGCGGAGCAGCCGGCGCCTGCGCCCGATACCTCGGCACCCGTCGTCGCACCGCGGGCAGCCATCG
AGGTCATCGGCGAACTGCTTGCGCGCGTGCTGACGCGTGGGGTCGCGCCGGTCGTTGCGCATGAAGCGGAACTGAGCGCT
GAAGTCGCGGTACTCGCGGCGGAGGCAACCAGCCTGGCCGACGAAGGCAGCGCGCTGCGCTACCGCGAACGCCTCGACAA
CTTCGCCGCCCGCCTCGCCTGGTACGGCGACGACCAGCACGCGGTGCGCGACGCCCTGCTGACGCTGCTGCGGCTGCTGG
TGGACAACATCCGTGAGCTGGTGCTGGACGACAACTGGCTGCACGGCCAGCTCAGCGTGCTCGCCGAGGCCTTCACCGGC
GAGCTGGATATCCGCATGCTCGACGAAGTCGAACGCCGCCTGCGCGACGTCATCGACAAGCAAAGCCACCTCAAACGCCA
GCTGTCGGACGCGCAGCAGCGGCTGAAGACCATGCTGGCCGGCTTCATCGACCGGCTCGCCAGCTTCTCCAACACCACCG
GCGAATTCCACGACGTGCTCGACCAGTGCGCCACCCGCATCAGCGCCGCGCACGACATCAGCGAGCTGTCGGATGTGGTC
GAGCAGATGCTGCGCGAAACGCGCAACACGCAGGAGTCGGCACGCCGTTCCGGCGAAGAACTGGCCGAACTGCGCGAACA
GGTGGAGTCGGCGAACCAGCACATCCTGCGCCTGCAGCGCGAACTCGACGAGACCAGCGAACTCGTGCGGCACGACCCGC
TGACCGGCGCGCTCAACCGCAAGGGCCTGGACGAAGCACTGGCACGCGAGATCGCACGCGCGCGGCGCCACGGCAGCCCG
CTCTGCCTCGCGCTGCTCGACGTCGACAACTTCAAGCAGCTGAACGACACCTACGGCCATCGCGCCGGCGACGACGCGCT
CCGGCACCTGTTCAGCGTGATCCGCGAAGCCGTGCGCCCGCAGGACGCGGTCGGCCGCTACGGCGGCGAGGAGTTCCTGA
TCGTGCTGCCGGACACCGACACCGAACAGGCGGCCGCCGTGGTCGTCCGCCTGCAGCGCGAACTGACGCGCCGCTTCTTC
CTCGCCAACTCGCAGAAGCTGCTGATCACCTTCAGCGCCGGCATCGCCCGCCTGCATGCCGACGAAGACCCGCAGCTCGC
GATCGACCGCGCCGACAAGGCCATGTACGCAGCCAAGCGCGCCGGCAAGAACCGGGTGCTCATCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCTAGCCCGCGCGCGCGCGTGGTTTTCGCCGCGGCGCGCGTGCATAATCCGGGCGACCGACCGGTTTTGTTTGCCGGC
GCGCCTTTCCGGATGGCTTG

Downstream 100 bases:

>100_bases
ACGCCATCGTCCGTCGTCCGGCCCCATGTCCGCACCACTCAATCCATCCGAAGTCGCGCGCGAAGTCCTGCTGCAACTGG
CCCAGCGCCGCATCCCGCCG

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTRTPPSAFPPSRPGGDGRRPGTTAETGTAPEIAREALRQLAAQRVPPTPDNYRAFYHQIAGTAPEEVFPTRALKAIAGA
LPRSTPEAVRLARQFEEAVAAGQWPALKLAIVAICETRGEAQLAWGLLIRDLVGQLERNQQGFTRARKLESLQHVLAGSS
ADPAQLHMRLQGLLRSWGQAAEGEEEALPGGPAEQPAPAPDTSAPVVAPRAAIEVIGELLARVLTRGVAPVVAHEAELSA
EVAVLAAEATSLADEGSALRYRERLDNFAARLAWYGDDQHAVRDALLTLLRLLVDNIRELVLDDNWLHGQLSVLAEAFTG
ELDIRMLDEVERRLRDVIDKQSHLKRQLSDAQQRLKTMLAGFIDRLASFSNTTGEFHDVLDQCATRISAAHDISELSDVV
EQMLRETRNTQESARRSGEELAELREQVESANQHILRLQRELDETSELVRHDPLTGALNRKGLDEALAREIARARRHGSP
LCLALLDVDNFKQLNDTYGHRAGDDALRHLFSVIREAVRPQDAVGRYGGEEFLIVLPDTDTEQAAAVVVRLQRELTRRFF
LANSQKLLITFSAGIARLHADEDPQLAIDRADKAMYAAKRAGKNRVLIAA

Sequences:

>Translated_610_residues
MTRTPPSAFPPSRPGGDGRRPGTTAETGTAPEIAREALRQLAAQRVPPTPDNYRAFYHQIAGTAPEEVFPTRALKAIAGA
LPRSTPEAVRLARQFEEAVAAGQWPALKLAIVAICETRGEAQLAWGLLIRDLVGQLERNQQGFTRARKLESLQHVLAGSS
ADPAQLHMRLQGLLRSWGQAAEGEEEALPGGPAEQPAPAPDTSAPVVAPRAAIEVIGELLARVLTRGVAPVVAHEAELSA
EVAVLAAEATSLADEGSALRYRERLDNFAARLAWYGDDQHAVRDALLTLLRLLVDNIRELVLDDNWLHGQLSVLAEAFTG
ELDIRMLDEVERRLRDVIDKQSHLKRQLSDAQQRLKTMLAGFIDRLASFSNTTGEFHDVLDQCATRISAAHDISELSDVV
EQMLRETRNTQESARRSGEELAELREQVESANQHILRLQRELDETSELVRHDPLTGALNRKGLDEALAREIARARRHGSP
LCLALLDVDNFKQLNDTYGHRAGDDALRHLFSVIREAVRPQDAVGRYGGEEFLIVLPDTDTEQAAAVVVRLQRELTRRFF
LANSQKLLITFSAGIARLHADEDPQLAIDRADKAMYAAKRAGKNRVLIAA
>Mature_609_residues
TRTPPSAFPPSRPGGDGRRPGTTAETGTAPEIAREALRQLAAQRVPPTPDNYRAFYHQIAGTAPEEVFPTRALKAIAGAL
PRSTPEAVRLARQFEEAVAAGQWPALKLAIVAICETRGEAQLAWGLLIRDLVGQLERNQQGFTRARKLESLQHVLAGSSA
DPAQLHMRLQGLLRSWGQAAEGEEEALPGGPAEQPAPAPDTSAPVVAPRAAIEVIGELLARVLTRGVAPVVAHEAELSAE
VAVLAAEATSLADEGSALRYRERLDNFAARLAWYGDDQHAVRDALLTLLRLLVDNIRELVLDDNWLHGQLSVLAEAFTGE
LDIRMLDEVERRLRDVIDKQSHLKRQLSDAQQRLKTMLAGFIDRLASFSNTTGEFHDVLDQCATRISAAHDISELSDVVE
QMLRETRNTQESARRSGEELAELREQVESANQHILRLQRELDETSELVRHDPLTGALNRKGLDEALAREIARARRHGSPL
CLALLDVDNFKQLNDTYGHRAGDDALRHLFSVIREAVRPQDAVGRYGGEEFLIVLPDTDTEQAAAVVVRLQRELTRRFFL
ANSQKLLITFSAGIARLHADEDPQLAIDRADKAMYAAKRAGKNRVLIAA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=219, Percent_Identity=36.0730593607306, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=35.5029585798817, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=34.7560975609756, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=37.7358490566038, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=37.888198757764, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=33.1491712707182, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=32.0224719101124, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788085, Length=198, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=220, Percent_Identity=30.4545454545455, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 67114; Mature: 66982

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTPPSAFPPSRPGGDGRRPGTTAETGTAPEIAREALRQLAAQRVPPTPDNYRAFYHQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AGTAPEEVFPTRALKAIAGALPRSTPEAVRLARQFEEAVAAGQWPALKLAIVAICETRGE
HCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
AQLAWGLLIRDLVGQLERNQQGFTRARKLESLQHVLAGSSADPAQLHMRLQGLLRSWGQA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
AEGEEEALPGGPAEQPAPAPDTSAPVVAPRAAIEVIGELLARVLTRGVAPVVAHEAELSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EVAVLAAEATSLADEGSALRYRERLDNFAARLAWYGDDQHAVRDALLTLLRLLVDNIREL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VLDDNWLHGQLSVLAEAFTGELDIRMLDEVERRLRDVIDKQSHLKRQLSDAQQRLKTMLA
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFIDRLASFSNTTGEFHDVLDQCATRISAAHDISELSDVVEQMLRETRNTQESARRSGEE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHH
LAELREQVESANQHILRLQRELDETSELVRHDPLTGALNRKGLDEALAREIARARRHGSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCC
LCLALLDVDNFKQLNDTYGHRAGDDALRHLFSVIREAVRPQDAVGRYGGEEFLIVLPDTD
EEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCC
TEQAAAVVVRLQRELTRRFFLANSQKLLITFSAGIARLHADEDPQLAIDRADKAMYAAKR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHH
AGKNRVLIAA
CCCCCEEEEC
>Mature Secondary Structure 
TRTPPSAFPPSRPGGDGRRPGTTAETGTAPEIAREALRQLAAQRVPPTPDNYRAFYHQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AGTAPEEVFPTRALKAIAGALPRSTPEAVRLARQFEEAVAAGQWPALKLAIVAICETRGE
HCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
AQLAWGLLIRDLVGQLERNQQGFTRARKLESLQHVLAGSSADPAQLHMRLQGLLRSWGQA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
AEGEEEALPGGPAEQPAPAPDTSAPVVAPRAAIEVIGELLARVLTRGVAPVVAHEAELSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EVAVLAAEATSLADEGSALRYRERLDNFAARLAWYGDDQHAVRDALLTLLRLLVDNIREL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VLDDNWLHGQLSVLAEAFTGELDIRMLDEVERRLRDVIDKQSHLKRQLSDAQQRLKTMLA
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFIDRLASFSNTTGEFHDVLDQCATRISAAHDISELSDVVEQMLRETRNTQESARRSGEE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHH
LAELREQVESANQHILRLQRELDETSELVRHDPLTGALNRKGLDEALAREIARARRHGSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCC
LCLALLDVDNFKQLNDTYGHRAGDDALRHLFSVIREAVRPQDAVGRYGGEEFLIVLPDTD
EEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCC
TEQAAAVVVRLQRELTRRFFLANSQKLLITFSAGIARLHADEDPQLAIDRADKAMYAAKR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHH
AGKNRVLIAA
CCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]