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The map label for this gene is ydeH [H]

Identifier: 119898982

GI number: 119898982

Start: 2967068

End: 2968882

Strand: Direct

Name: ydeH [H]

Synonym: azo2692

Alternate gene names: 119898982

Gene position: 2967068-2968882 (Clockwise)

Preceding gene: 119898981

Following gene: 119898983

Centisome position: 67.8

GC content: 69.92

Gene sequence:

>1815_bases
ATGTCCGCACCACTCAATCCATCCGAAGTCGCGCGCGAAGTCCTGCTGCAACTGGCCCAGCGCCGCATCCCGCCGACGCC
GGACAACTACCAGCGCCTGTACTACGAGATCGCCGGCGGCGAGGCGGCCGAGGCGCCTTTTCCCGAGCGCTATGTCGCCC
GCCTGGCCCGCCGCCTGCCGCGCGACAACGCGGAACGCCAGCGCCTTGCGCGCCAGCTGGAACAGGCCCTGGCAGGCGGT
GACGGCGCCGCGGCGGAAGCCGCGCTGGGCCAGTACCTCGACGCCCTCGCGATCGAGCCTCCACCCGCCTGGAACGAGTT
GATCGCGCGCCTGCTGCGGCAGTGGGAAGGGCGCCAGCTCGGCTGGACCACCGCGCGCAAGCGCGAGTCGCTGGAGCGCG
TGCTCGCCGCCAGCGACCCCGCCACGCTCTACGCCCGCCTGCAGGCCTTGCTCGGAGCCTGGGGCCAGACGCCGACCGAC
CCCGAACTGCCGCCCACGCCGCACGTTCCCGACCTGCCCTCCGCCCCCGCGGCGGCCACGGCCGAGGGCAACGGCGGCAC
GGCGGCGACGGAAGTACGGCTGCTCGGCCGCGGCGAATCCGTCGAGCTGCTCGCAAACCTGCGCGAACTGCTGCTGCTGA
CACTCGATACCGTCGTGCCCGCCATGCTCGGCGACGACGCGGCGCTGCTGCAGGATGCGCGCGAGATCGCCACCACGGTG
CGCGGCGCGGCCGACCGGCCCGCGCTCGATGTCGGCGCGCGCCAGTTGCGCAAGTTCGCGCTGCGGCTGGAAGGCCACGC
GGCCGACAACGCCGAGCTTCGCAACGGCCTTCTCGACCTGCTGCGTCTGCTGCTGACCAACATCGACGAACTGGTGCTGG
ACGACCAATGGTTGCACGGCCAGGTCGAAACCCTGCGCACCGTCCTCGACCAGACCCTCGACGTGCGCGTGCTCGACAAC
GCGGAGCGCCAGCTCAAGGAAGTGATCTACAAGCAGAGCCAGCTCAAGCACAACCTCGTCGAAGCGCAGCGCAACCTCAA
GGCCATGCTGGCGGGCTTCGTCGATCAGCTCGCCCGCTTCAGCGCCTCCACCGACACCTACCAGGGCAAGCTCGGCGACT
GCGCGCAGAAGATCGCCAACGCCCGCGACATCACCGAGATCGGCCACCTGCTCGACGAAGTCATGGCCGAAACCATCGCC
ATCCGCGACGAGACCCGGCGCTCGCACGACGAACTGCTGACCACGCGCGAACGGGTCGAGAGCGCCGAAGCGCAGATGGC
CGCGATGCAGCGCGCGCTGGACGAAGCCAGCCGGCTGATGCGCCACGACACCCTCACCAACGCCCTCAACCGGCGCGGGC
TGGAAGACATGTTCGGCAAGGAGTCGTCCCGCGCCCAACGCCACCACACCGGCCTGTGCCTGGCGCTGCTCGATCTCGAC
AACTTCAAGCGCCTCAACGACACCCTCGGCCACAGCAGCGGTGACGGCGCGCTGCTGCACCTTGCCACCGTCGTGCGCCG
CCTGCTGCGTCCGCACGACACCCTGGCCCGCTATGGCGGCGAGGAGTTCGTGATCCTCTACCCCGACGCGGATCTCGCGC
AGGCGGAAGCGGCGCTGATCCGCCTGCAGCGCGAACTGACGCGCAGCTTCTTCATGTCCAACCAGCAGAAGATCGTCATC
ACCTTCAGCGCCGGTGTCACCGCCGTGATCCTCGGCGAACCGCTGGAGCAGGCACTCGCGCGCGCCGATGCGGCGATGTA
CCGCGCGAAGCAGAACGGCAAGAACCAGGTGGTTGCCGCACCGGCAAACCCGTAG

Upstream 100 bases:

>100_bases
GCGATCGACCGCGCCGACAAGGCCATGTACGCAGCCAAGCGCGCCGGCAAGAACCGGGTGCTCATCGCGGCCTGAACGCC
ATCGTCCGTCGTCCGGCCCC

Downstream 100 bases:

>100_bases
GCCGCCCGCCGGCAAAACGCCGGCAAGCCCTGGCAATAGTTGCCGCCTCGATCCGGCAACAACTCCCAACACATTGAAAT
TTAAGTAGTAAATCCGCACA

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MSAPLNPSEVAREVLLQLAQRRIPPTPDNYQRLYYEIAGGEAAEAPFPERYVARLARRLPRDNAERQRLARQLEQALAGG
DGAAAEAALGQYLDALAIEPPPAWNELIARLLRQWEGRQLGWTTARKRESLERVLAASDPATLYARLQALLGAWGQTPTD
PELPPTPHVPDLPSAPAAATAEGNGGTAATEVRLLGRGESVELLANLRELLLLTLDTVVPAMLGDDAALLQDAREIATTV
RGAADRPALDVGARQLRKFALRLEGHAADNAELRNGLLDLLRLLLTNIDELVLDDQWLHGQVETLRTVLDQTLDVRVLDN
AERQLKEVIYKQSQLKHNLVEAQRNLKAMLAGFVDQLARFSASTDTYQGKLGDCAQKIANARDITEIGHLLDEVMAETIA
IRDETRRSHDELLTTRERVESAEAQMAAMQRALDEASRLMRHDTLTNALNRRGLEDMFGKESSRAQRHHTGLCLALLDLD
NFKRLNDTLGHSSGDGALLHLATVVRRLLRPHDTLARYGGEEFVILYPDADLAQAEAALIRLQRELTRSFFMSNQQKIVI
TFSAGVTAVILGEPLEQALARADAAMYRAKQNGKNQVVAAPANP

Sequences:

>Translated_604_residues
MSAPLNPSEVAREVLLQLAQRRIPPTPDNYQRLYYEIAGGEAAEAPFPERYVARLARRLPRDNAERQRLARQLEQALAGG
DGAAAEAALGQYLDALAIEPPPAWNELIARLLRQWEGRQLGWTTARKRESLERVLAASDPATLYARLQALLGAWGQTPTD
PELPPTPHVPDLPSAPAAATAEGNGGTAATEVRLLGRGESVELLANLRELLLLTLDTVVPAMLGDDAALLQDAREIATTV
RGAADRPALDVGARQLRKFALRLEGHAADNAELRNGLLDLLRLLLTNIDELVLDDQWLHGQVETLRTVLDQTLDVRVLDN
AERQLKEVIYKQSQLKHNLVEAQRNLKAMLAGFVDQLARFSASTDTYQGKLGDCAQKIANARDITEIGHLLDEVMAETIA
IRDETRRSHDELLTTRERVESAEAQMAAMQRALDEASRLMRHDTLTNALNRRGLEDMFGKESSRAQRHHTGLCLALLDLD
NFKRLNDTLGHSSGDGALLHLATVVRRLLRPHDTLARYGGEEFVILYPDADLAQAEAALIRLQRELTRSFFMSNQQKIVI
TFSAGVTAVILGEPLEQALARADAAMYRAKQNGKNQVVAAPANP
>Mature_603_residues
SAPLNPSEVAREVLLQLAQRRIPPTPDNYQRLYYEIAGGEAAEAPFPERYVARLARRLPRDNAERQRLARQLEQALAGGD
GAAAEAALGQYLDALAIEPPPAWNELIARLLRQWEGRQLGWTTARKRESLERVLAASDPATLYARLQALLGAWGQTPTDP
ELPPTPHVPDLPSAPAAATAEGNGGTAATEVRLLGRGESVELLANLRELLLLTLDTVVPAMLGDDAALLQDAREIATTVR
GAADRPALDVGARQLRKFALRLEGHAADNAELRNGLLDLLRLLLTNIDELVLDDQWLHGQVETLRTVLDQTLDVRVLDNA
ERQLKEVIYKQSQLKHNLVEAQRNLKAMLAGFVDQLARFSASTDTYQGKLGDCAQKIANARDITEIGHLLDEVMAETIAI
RDETRRSHDELLTTRERVESAEAQMAAMQRALDEASRLMRHDTLTNALNRRGLEDMFGKESSRAQRHHTGLCLALLDLDN
FKRLNDTLGHSSGDGALLHLATVVRRLLRPHDTLARYGGEEFVILYPDADLAQAEAALIRLQRELTRSFFMSNQQKIVIT
FSAGVTAVILGEPLEQALARADAAMYRAKQNGKNQVVAAPANP

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=38.4615384615385, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI145693134, Length=229, Percent_Identity=31.8777292576419, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=33.1491712707182, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=31.6455696202532, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=34.5679012345679, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=35.1190476190476, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=32.5714285714286, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=33.5227272727273, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=26.5536723163842, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=29.6969696969697, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 66558; Mature: 66427

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPLNPSEVAREVLLQLAQRRIPPTPDNYQRLYYEIAGGEAAEAPFPERYVARLARRLP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC
RDNAERQRLARQLEQALAGGDGAAAEAALGQYLDALAIEPPPAWNELIARLLRQWEGRQL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GWTTARKRESLERVLAASDPATLYARLQALLGAWGQTPTDPELPPTPHVPDLPSAPAAAT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AEGNGGTAATEVRLLGRGESVELLANLRELLLLTLDTVVPAMLGDDAALLQDAREIATTV
CCCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGAADRPALDVGARQLRKFALRLEGHAADNAELRNGLLDLLRLLLTNIDELVLDDQWLHG
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHH
QVETLRTVLDQTLDVRVLDNAERQLKEVIYKQSQLKHNLVEAQRNLKAMLAGFVDQLARF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASTDTYQGKLGDCAQKIANARDITEIGHLLDEVMAETIAIRDETRRSHDELLTTRERVE
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEAQMAAMQRALDEASRLMRHDTLTNALNRRGLEDMFGKESSRAQRHHTGLCLALLDLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHEECCCH
NFKRLNDTLGHSSGDGALLHLATVVRRLLRPHDTLARYGGEEFVILYPDADLAQAEAALI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RLQRELTRSFFMSNQQKIVITFSAGVTAVILGEPLEQALARADAAMYRAKQNGKNQVVAA
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEC
PANP
CCCC
>Mature Secondary Structure 
SAPLNPSEVAREVLLQLAQRRIPPTPDNYQRLYYEIAGGEAAEAPFPERYVARLARRLP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC
RDNAERQRLARQLEQALAGGDGAAAEAALGQYLDALAIEPPPAWNELIARLLRQWEGRQL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GWTTARKRESLERVLAASDPATLYARLQALLGAWGQTPTDPELPPTPHVPDLPSAPAAAT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AEGNGGTAATEVRLLGRGESVELLANLRELLLLTLDTVVPAMLGDDAALLQDAREIATTV
CCCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGAADRPALDVGARQLRKFALRLEGHAADNAELRNGLLDLLRLLLTNIDELVLDDQWLHG
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHH
QVETLRTVLDQTLDVRVLDNAERQLKEVIYKQSQLKHNLVEAQRNLKAMLAGFVDQLARF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASTDTYQGKLGDCAQKIANARDITEIGHLLDEVMAETIAIRDETRRSHDELLTTRERVE
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEAQMAAMQRALDEASRLMRHDTLTNALNRRGLEDMFGKESSRAQRHHTGLCLALLDLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHEECCCH
NFKRLNDTLGHSSGDGALLHLATVVRRLLRPHDTLARYGGEEFVILYPDADLAQAEAALI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RLQRELTRSFFMSNQQKIVITFSAGVTAVILGEPLEQALARADAAMYRAKQNGKNQVVAA
HHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEC
PANP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]