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The map label for this gene is hflB [C]

Identifier: 119898929

GI number: 119898929

Start: 2916466

End: 2918697

Strand: Reverse

Name: hflB [C]

Synonym: azo2638

Alternate gene names: 119898929

Gene position: 2918697-2916466 (Counterclockwise)

Preceding gene: 119898930

Following gene: 119898928

Centisome position: 66.7

GC content: 65.28

Gene sequence:

>2232_bases
ATGAGCCGGATGCGTAAGGCTGCCCTGGGTAGGCCGTGGCGCAAGGATTTCACGCAAGTGGAGCCCGTGGCACGGCGGAC
TGCGCGTTATGCCCTGCGGATGCTCGCCTGCTGCCCGATGCTGCACAAGCGGCTGAGCGACGATGATTTTCTGGGGCCGC
TGTGGGTCATGACCGTGCCCCTTCTCGACCCCGCCAAACTCGCTGAACTCGAGGCGGAGTGGAGCCGTACCGACGGTGAG
GAAGGAGCGGTGTCGGGAGATGAGGATGTTCTCGACGGCCAATGGTTTCAGGTGCTCGACATGCACAACGGTTATCGATT
TCCCCGCGGCGTGCCCGGGGCACGCATGCGCGACTACCTGGTGACGCTATTCAGCAGCATTCCCGACAGCGTGCTCGCCC
GACTGACGCGCGACGATGGTATCGCACCAACGCATCCCAGCGTGGCGGTGCTCGCCGAGTGTGCCGGGCTGAATGAAATC
GAGCGCCATGTTCTCGATTTCATCGAGAAAAAGGAGAACGTGGCTCCGTTCCGCCCCTTCCTTCGCGAAACGGGGTGCGA
GGCTTCGCGCGATCGCTACGCGTGCCTCGCGGCCGCGCTCGATGTCACGACGGTTCGTCTGCGGCAGAATCTGGGCCGCA
AGAGCGCACTTGCGATTCTGCATCTGGCCAAGCGAGCTGGACGACGTTCGGATCTGGAGGACTTCGTGCAGGCTGGCGAT
TTGTTCGAGGACATCCTCGGGTTGGAGCCGACGACGCCAGAGGGCTTGTTGGACGCCGTGGTCGAGCCGGCCACCGTCGC
CGACTGCATGCTTCAGGACTTCCCGCACCTCGCGGCGGATGGGGCCCGGCTGTGCGGCGTTCTCGGCAAGGCGGCGGCGA
CCCGCGTCGTCGGGGTCAACGCACTGTTCTATGGCCCGCCGGGCAGCGGAAAAACCCAGTTCGCCAGCGCGGTGGCGCTG
GCGGCAGGGTTGAAGGCATATCGCGTCAAGTCTGCCGATGACGACGGCGACGGTTTGTCGCGCGCCGGGCGACTGGGGGC
CTACCAGCTCACCCAGCGTCTGCTGCGCGGCCGTAGCGACTGCGTCGTGGTCTTCGACGAAGTCGAGGATGCGTTCGGCA
ACGCCGAAAGCGCGCTGCTGGCCTACTTCGCGGGCCATGCCAAGGCGGGCGGGGACAAGGGGTGGATGAACCGAACCCTG
GAGGACAACCCGCTGCCGGCAATCTGGATCACCAACGACGTCGAGAGCATGGATCCGGCCTTCCTGCGGCGCTTCCTGCT
GCCGGTGGCTTTCTCGACGCCGCCGCGCCGGGTACGTCGCCAGATGATCGAGCGCCATCTGGGCGGCACGGCGGTGTCGG
CAGCAGCGCTGGACGAGCTTGCTGCCGATTCTGCCCTGATGCCTGCCCAGTACGGTGCGGCGCGTCGCTTGCTCGACCTG
TGCGAGGGCGCGGAGCCCGAATCGGTTGTGCGCGAGGGTATCGCCGCCGCACGGCGGGTGCTGCACGGCGCGGTAGGGCC
GCGTCCGCGCCGGCCGGCGACGACCTTCGATGTCGCCTACCTCAACATTGCCGGAGGACCCAGCCCGTCGCGGCTGGCCG
ATGCGCTGGCTCGCAATGGTCGTGGTGCGCTGTGCTTCTTCGGTCCGCCGGGCACTGGCAAGACCGAGTTTGCCCATGTG
CTGGCCGATGCACTCGACCGCGAGTTGGTGGTCCAGGCAAGTTCCGATCTCGTCTCGCCCTACGTCGGCGAAACCGAACG
CAATATCGCTCGCCTGTTTGCAGGAATCGACACCGGACACAGCGTGCTGCTGATCGACGAAGTCGACAGCCTGCTGCGTG
ACCGAAGTCACGCCCAGCGCAGTTGGGAGGCCACTCAGGTGAATGAGTTGCTGCAACAGATTGAGCGTTACCACGGCATC
TTCATTGCGGCTACCAATCTGATGAGCCAGCTCGATGGCGCAGCCATGCGGCGGTTCGACTTCAAGCTGTCGTTCAGGCC
GCTGAGCCGGGCGCAGCGTCTGGCGCTGTTTGCACGCGAGGTATTGGGGGCCGCGGATAGGGTCGCTGCGATTCCTCACG
CGCTGGTGGCTCGCATCGACACCCTGGAGATGCTGACTCCGGGTGACTTTGCCAATGTCGTGCGCCAGCGCGAATTGCTC
GGAGAGGAACTGAGTCCTGAGGAGTTTTTACGCCAACTGATTGTGGAGTGCCGGTACAAAGACGGGCGGTAG

Upstream 100 bases:

>100_bases
TGCTGGGCCTCTTTCTGGACGAAGGCGAGCATCGGGGCGATTTCCTGAGTTGGCACTTCGACAGCCGGCGCTTTCAACCC
TGGACCGAAGGAGAGTGCAA

Downstream 100 bases:

>100_bases
GCGCGTATTGGCTTTTTTGGGGAACAACGCTTTCGCAGAGGGTGAAGGAGTGACACAGGATGTCTGCAATTTTTACTGCA
CTTAACGCCGATATCACGAC

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSRMRKAALGRPWRKDFTQVEPVARRTARYALRMLACCPMLHKRLSDDDFLGPLWVMTVPLLDPAKLAELEAEWSRTDGE
EGAVSGDEDVLDGQWFQVLDMHNGYRFPRGVPGARMRDYLVTLFSSIPDSVLARLTRDDGIAPTHPSVAVLAECAGLNEI
ERHVLDFIEKKENVAPFRPFLRETGCEASRDRYACLAAALDVTTVRLRQNLGRKSALAILHLAKRAGRRSDLEDFVQAGD
LFEDILGLEPTTPEGLLDAVVEPATVADCMLQDFPHLAADGARLCGVLGKAAATRVVGVNALFYGPPGSGKTQFASAVAL
AAGLKAYRVKSADDDGDGLSRAGRLGAYQLTQRLLRGRSDCVVVFDEVEDAFGNAESALLAYFAGHAKAGGDKGWMNRTL
EDNPLPAIWITNDVESMDPAFLRRFLLPVAFSTPPRRVRRQMIERHLGGTAVSAAALDELAADSALMPAQYGAARRLLDL
CEGAEPESVVREGIAAARRVLHGAVGPRPRRPATTFDVAYLNIAGGPSPSRLADALARNGRGALCFFGPPGTGKTEFAHV
LADALDRELVVQASSDLVSPYVGETERNIARLFAGIDTGHSVLLIDEVDSLLRDRSHAQRSWEATQVNELLQQIERYHGI
FIAATNLMSQLDGAAMRRFDFKLSFRPLSRAQRLALFAREVLGAADRVAAIPHALVARIDTLEMLTPGDFANVVRQRELL
GEELSPEEFLRQLIVECRYKDGR

Sequences:

>Translated_743_residues
MSRMRKAALGRPWRKDFTQVEPVARRTARYALRMLACCPMLHKRLSDDDFLGPLWVMTVPLLDPAKLAELEAEWSRTDGE
EGAVSGDEDVLDGQWFQVLDMHNGYRFPRGVPGARMRDYLVTLFSSIPDSVLARLTRDDGIAPTHPSVAVLAECAGLNEI
ERHVLDFIEKKENVAPFRPFLRETGCEASRDRYACLAAALDVTTVRLRQNLGRKSALAILHLAKRAGRRSDLEDFVQAGD
LFEDILGLEPTTPEGLLDAVVEPATVADCMLQDFPHLAADGARLCGVLGKAAATRVVGVNALFYGPPGSGKTQFASAVAL
AAGLKAYRVKSADDDGDGLSRAGRLGAYQLTQRLLRGRSDCVVVFDEVEDAFGNAESALLAYFAGHAKAGGDKGWMNRTL
EDNPLPAIWITNDVESMDPAFLRRFLLPVAFSTPPRRVRRQMIERHLGGTAVSAAALDELAADSALMPAQYGAARRLLDL
CEGAEPESVVREGIAAARRVLHGAVGPRPRRPATTFDVAYLNIAGGPSPSRLADALARNGRGALCFFGPPGTGKTEFAHV
LADALDRELVVQASSDLVSPYVGETERNIARLFAGIDTGHSVLLIDEVDSLLRDRSHAQRSWEATQVNELLQQIERYHGI
FIAATNLMSQLDGAAMRRFDFKLSFRPLSRAQRLALFAREVLGAADRVAAIPHALVARIDTLEMLTPGDFANVVRQRELL
GEELSPEEFLRQLIVECRYKDGR
>Mature_742_residues
SRMRKAALGRPWRKDFTQVEPVARRTARYALRMLACCPMLHKRLSDDDFLGPLWVMTVPLLDPAKLAELEAEWSRTDGEE
GAVSGDEDVLDGQWFQVLDMHNGYRFPRGVPGARMRDYLVTLFSSIPDSVLARLTRDDGIAPTHPSVAVLAECAGLNEIE
RHVLDFIEKKENVAPFRPFLRETGCEASRDRYACLAAALDVTTVRLRQNLGRKSALAILHLAKRAGRRSDLEDFVQAGDL
FEDILGLEPTTPEGLLDAVVEPATVADCMLQDFPHLAADGARLCGVLGKAAATRVVGVNALFYGPPGSGKTQFASAVALA
AGLKAYRVKSADDDGDGLSRAGRLGAYQLTQRLLRGRSDCVVVFDEVEDAFGNAESALLAYFAGHAKAGGDKGWMNRTLE
DNPLPAIWITNDVESMDPAFLRRFLLPVAFSTPPRRVRRQMIERHLGGTAVSAAALDELAADSALMPAQYGAARRLLDLC
EGAEPESVVREGIAAARRVLHGAVGPRPRRPATTFDVAYLNIAGGPSPSRLADALARNGRGALCFFGPPGTGKTEFAHVL
ADALDRELVVQASSDLVSPYVGETERNIARLFAGIDTGHSVLLIDEVDSLLRDRSHAQRSWEATQVNELLQQIERYHGIF
IAATNLMSQLDGAAMRRFDFKLSFRPLSRAQRLALFAREVLGAADRVAAIPHALVARIDTLEMLTPGDFANVVRQRELLG
EELSPEEFLRQLIVECRYKDGR

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17532375, Length=124, Percent_Identity=36.2903225806452, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537217, Length=119, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325431, Length=150, Percent_Identity=32.6666666666667, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 81294; Mature: 81163

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRMRKAALGRPWRKDFTQVEPVARRTARYALRMLACCPMLHKRLSDDDFLGPLWVMTVP
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCC
LLDPAKLAELEAEWSRTDGEEGAVSGDEDVLDGQWFQVLDMHNGYRFPRGVPGARMRDYL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
VTLFSSIPDSVLARLTRDDGIAPTHPSVAVLAECAGLNEIERHVLDFIEKKENVAPFRPF
HHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
LRETGCEASRDRYACLAAALDVTTVRLRQNLGRKSALAILHLAKRAGRRSDLEDFVQAGD
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LFEDILGLEPTTPEGLLDAVVEPATVADCMLQDFPHLAADGARLCGVLGKAAATRVVGVN
HHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEE
ALFYGPPGSGKTQFASAVALAAGLKAYRVKSADDDGDGLSRAGRLGAYQLTQRLLRGRSD
EEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCCC
CVVVFDEVEDAFGNAESALLAYFAGHAKAGGDKGWMNRTLEDNPLPAIWITNDVESMDPA
EEEEEHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCHH
FLRRFLLPVAFSTPPRRVRRQMIERHLGGTAVSAAALDELAADSALMPAQYGAARRLLDL
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CEGAEPESVVREGIAAARRVLHGAVGPRPRRPATTFDVAYLNIAGGPSPSRLADALARNG
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCC
RGALCFFGPPGTGKTEFAHVLADALDRELVVQASSDLVSPYVGETERNIARLFAGIDTGH
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
SVLLIDEVDSLLRDRSHAQRSWEATQVNELLQQIERYHGIFIAATNLMSQLDGAAMRRFD
EEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FKLSFRPLSRAQRLALFAREVLGAADRVAAIPHALVARIDTLEMLTPGDFANVVRQRELL
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GEELSPEEFLRQLIVECRYKDGR
CCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]