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The map label for this gene is ligA1 [H]

Identifier: 119898814

GI number: 119898814

Start: 2789756

End: 2790106

Strand: Reverse

Name: ligA1 [H]

Synonym: azo2523

Alternate gene names: 119898814

Gene position: 2790106-2789756 (Counterclockwise)

Preceding gene: 119898817

Following gene: 119898813

Centisome position: 63.76

GC content: 63.82

Gene sequence:

>351_bases
ATGGCCACACGGCAGAAACGCGAGATTCCCGGCACGTCGATCTTCGACGGCGAACTGGCGCAGAAGGGCTACGCGCTCAA
CAAGATGTGCTTCTCGTTCAACAGCGCGGACAACCGCGCCGAGTTCCAGCGCGACGAGGCGGCCTACTGCCGCAAGTTCG
GCCTCAACGACGAACAGGAAACCGCCATCCGCACGCGCAATGTGCTCGGCCTGCTGGCCGCGGGCGGCAACGTGTATTAC
CTGGCGAAGTTCGCCGGCATTCTGGGACTCGACGTGCAGGACCTGGGCGCTGCCCAGACCGGCATGACCAAGGAAGCGTT
CAAGGCCATGCTCGTGGCCGCGGGGAAATAA

Upstream 100 bases:

>100_bases
CGAATTTGCTCATGATTGGAATGAGTTTCCCTGTGTTTATCCGTGAAGTCCAGCCGCCGATACTGATAACCCCACTCACA
ACAAGGACGAGGAGAGGCGG

Downstream 100 bases:

>100_bases
GACATGGCAAAGATCGTAGGCACCATCACCACTTCGCACGTCCCGGCCATCGGCCGCGCCCTCGCCGGCAAGCTGCAGGA
CGACCCGTACTGGAAGCCTT

Product: protocatechuate 4,5-dioxygenase subunit alpha

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MATRQKREIPGTSIFDGELAQKGYALNKMCFSFNSADNRAEFQRDEAAYCRKFGLNDEQETAIRTRNVLGLLAAGGNVYY
LAKFAGILGLDVQDLGAAQTGMTKEAFKAMLVAAGK

Sequences:

>Translated_116_residues
MATRQKREIPGTSIFDGELAQKGYALNKMCFSFNSADNRAEFQRDEAAYCRKFGLNDEQETAIRTRNVLGLLAAGGNVYY
LAKFAGILGLDVQDLGAAQTGMTKEAFKAMLVAAGK
>Mature_115_residues
ATRQKREIPGTSIFDGELAQKGYALNKMCFSFNSADNRAEFQRDEAAYCRKFGLNDEQETAIRTRNVLGLLAAGGNVYYL
AKFAGILGLDVQDLGAAQTGMTKEAFKAMLVAAGK

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 12622; Mature: 12490

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRQKREIPGTSIFDGELAQKGYALNKMCFSFNSADNRAEFQRDEAAYCRKFGLNDEQE
CCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHH
TAIRTRNVLGLLAAGGNVYYLAKFAGILGLDVQDLGAAQTGMTKEAFKAMLVAAGK
HHHHHHHHHHEEECCCCEEEHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ATRQKREIPGTSIFDGELAQKGYALNKMCFSFNSADNRAEFQRDEAAYCRKFGLNDEQE
CCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHH
TAIRTRNVLGLLAAGGNVYYLAKFAGILGLDVQDLGAAQTGMTKEAFKAMLVAAGK
HHHHHHHHHHEEECCCCEEEHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]