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The map label for this gene is nagG [H]

Identifier: 119898807

GI number: 119898807

Start: 2781815

End: 2783131

Strand: Reverse

Name: nagG [H]

Synonym: azo2516

Alternate gene names: 119898807

Gene position: 2783131-2781815 (Counterclockwise)

Preceding gene: 119898808

Following gene: 119898806

Centisome position: 63.6

GC content: 65.91

Gene sequence:

>1317_bases
ATGACCACACCATCCACCGTATTCCCCACCGGCGTCAGCTGGGAAACCGCCGGCACCAGCCGCATCCCCTTCAAGGTCTA
CACCGACGAGGAAGTCCACAAGCAGGAACTCGAACGCTTCTTCTACAAGGGCCACTGGAGCTACGTCGGCCTGGAGGCGG
AGATCCCTGAGCCCGGCGACTTCAAGCGCACGGTGGTGGGCGAGCGGTCGGTGATCATGGTGCGCGACGCCGAGGGCGGC
ATCAGCGTGGTGGAGAACGTCTGTGCCCACCGCGGCATGCGCTTCTGCCGCGAGCGCCACGGCAACCGCAAGGAATTCGT
CTGTCCCTACCACCAGTGGAACTACACGCTGAAGGGCGACCTGCAGGGCGTGCCTTTCCGGCGCGGCGTGAAGCAGGATG
GCAAGGTCAATGGCGGCATGCCGGCGGACTTCAAGGTGGAGGACCACGGTCTCACCCAACTCAAGGTGGCCACCCGCGGC
GGCGTGGTGTTCGCCTCCTTCGACCACGAGGTCGAGCCGCTGGAGGACTACATCGGCCCGACCATCCTCCAGTACTTCGA
CCGCCTCTTCAACGGCCGCAAGCTGCGCATCCTCGGCTACAACCGGCAGCGCATTCCTGGCAACTGGAAGCTGATGCAGG
AGAACATCAAGGACCCGTACCACCCCGGTTTGCTGCACACCTGGTTCGTCACCTTCGGGCTCTGGCGTGCCGACAACAAG
TCCGAACTCAAGATGGACGCGCACGGCCGCCATGCGGCGATGATCTCGACGCGCGGGCAGGGCGGCAAGGCGGCGCAGGT
GACCCAGGTGCCGAGCTTCAAGGAGAGCATGCAGCTGAACGACCCGCGCTTCCTCGACATCGTGCCCGAGCCCTGGTGGA
ACGGCCCGACCGCGGTGATGACCACGCTGTTCCCGAGCGTGATCCTGCAGCAGCAGGTCAACAGCGTCTCCACCCGCCAC
ATCCAGCCCGACGGCCATGGTTCCTTCGACTTCGTGTGGACCCACTTCGCCTTCGAGGACGATGACGACGAGATGCTGCA
GCGCCGCCTGCGCCAGGCCAACCTTTTCGGCCCGGCCGGCTTCGTCTCCGCCGATGACGGCGAGGTCATCGAGTTCTCGC
AGGAAGGTTTCGAGCAGAAGCCCTTCCACCGCGCGCTGGCCGAACTCGGCGGCCGCGCGGTCGACCTCCACACCGACCAC
ATGGTCACCGAAACGCTGATCCGCGGCATGTACGGCTACTGGCGCCGGGTGATGGAGGAGGGCGGCGGGCCCCGCGGAAG
CACACCGCAGGCTGGCGGTGCGGAGGTGTCCCAATGA

Upstream 100 bases:

>100_bases
CCCGGCTACGCCCCCCTTGAAGGGGAGGGATCACCCCCGGCTAGCAGTCTCTGCGCTCACCCAAGGACACAAAGCACCAA
AACACAACGAGGAGACACCC

Downstream 100 bases:

>100_bases
GCAGCAACGCGATCGAAATGAACTTCGCCGACTGGTTCGAACTGACCAATCTCTACGCCGACTACGCCCGCGCGGTCGAT
TCCGGCGACTGGGACCTGTG

Product: putative ring hydroxylating large subunit

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTTPSTVFPTGVSWETAGTSRIPFKVYTDEEVHKQELERFFYKGHWSYVGLEAEIPEPGDFKRTVVGERSVIMVRDAEGG
ISVVENVCAHRGMRFCRERHGNRKEFVCPYHQWNYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKVEDHGLTQLKVATRG
GVVFASFDHEVEPLEDYIGPTILQYFDRLFNGRKLRILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
SELKMDAHGRHAAMISTRGQGGKAAQVTQVPSFKESMQLNDPRFLDIVPEPWWNGPTAVMTTLFPSVILQQQVNSVSTRH
IQPDGHGSFDFVWTHFAFEDDDDEMLQRRLRQANLFGPAGFVSADDGEVIEFSQEGFEQKPFHRALAELGGRAVDLHTDH
MVTETLIRGMYGYWRRVMEEGGGPRGSTPQAGGAEVSQ

Sequences:

>Translated_438_residues
MTTPSTVFPTGVSWETAGTSRIPFKVYTDEEVHKQELERFFYKGHWSYVGLEAEIPEPGDFKRTVVGERSVIMVRDAEGG
ISVVENVCAHRGMRFCRERHGNRKEFVCPYHQWNYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKVEDHGLTQLKVATRG
GVVFASFDHEVEPLEDYIGPTILQYFDRLFNGRKLRILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
SELKMDAHGRHAAMISTRGQGGKAAQVTQVPSFKESMQLNDPRFLDIVPEPWWNGPTAVMTTLFPSVILQQQVNSVSTRH
IQPDGHGSFDFVWTHFAFEDDDDEMLQRRLRQANLFGPAGFVSADDGEVIEFSQEGFEQKPFHRALAELGGRAVDLHTDH
MVTETLIRGMYGYWRRVMEEGGGPRGSTPQAGGAEVSQ
>Mature_437_residues
TTPSTVFPTGVSWETAGTSRIPFKVYTDEEVHKQELERFFYKGHWSYVGLEAEIPEPGDFKRTVVGERSVIMVRDAEGGI
SVVENVCAHRGMRFCRERHGNRKEFVCPYHQWNYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKVEDHGLTQLKVATRGG
VVFASFDHEVEPLEDYIGPTILQYFDRLFNGRKLRILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKS
ELKMDAHGRHAAMISTRGQGGKAAQVTQVPSFKESMQLNDPRFLDIVPEPWWNGPTAVMTTLFPSVILQQQVNSVSTRHI
QPDGHGSFDFVWTHFAFEDDDDEMLQRRLRQANLFGPAGFVSADDGEVIEFSQEGFEQKPFHRALAELGGRAVDLHTDHM
VTETLIRGMYGYWRRVMEEGGGPRGSTPQAGGAEVSQ

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=453, Percent_Identity=26.0485651214128, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788103, Length=228, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 49706; Mature: 49574

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSTVFPTGVSWETAGTSRIPFKVYTDEEVHKQELERFFYKGHWSYVGLEAEIPEPGD
CCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FKRTVVGERSVIMVRDAEGGISVVENVCAHRGMRFCRERHGNRKEFVCPYHQWNYTLKGD
CCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCEEEECC
LQGVPFRRGVKQDGKVNGGMPADFKVEDHGLTQLKVATRGGVVFASFDHEVEPLEDYIGP
CCCCCHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCEEEEECCCCCCHHHHHCCH
TILQYFDRLFNGRKLRILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCC
SELKMDAHGRHAAMISTRGQGGKAAQVTQVPSFKESMQLNDPRFLDIVPEPWWNGPTAVM
CCEEECCCCCEEEEEEECCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCCCCCHHHHH
TTLFPSVILQQQVNSVSTRHIQPDGHGSFDFVWTHFAFEDDDDEMLQRRLRQANLFGPAG
HHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
FVSADDGEVIEFSQEGFEQKPFHRALAELGGRAVDLHTDHMVTETLIRGMYGYWRRVMEE
CCCCCCCCEEEECCCCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
GGGPRGSTPQAGGAEVSQ
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPSTVFPTGVSWETAGTSRIPFKVYTDEEVHKQELERFFYKGHWSYVGLEAEIPEPGD
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FKRTVVGERSVIMVRDAEGGISVVENVCAHRGMRFCRERHGNRKEFVCPYHQWNYTLKGD
CCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCEEEECC
LQGVPFRRGVKQDGKVNGGMPADFKVEDHGLTQLKVATRGGVVFASFDHEVEPLEDYIGP
CCCCCHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCEEEEECCCCCCHHHHHCCH
TILQYFDRLFNGRKLRILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCC
SELKMDAHGRHAAMISTRGQGGKAAQVTQVPSFKESMQLNDPRFLDIVPEPWWNGPTAVM
CCEEECCCCCEEEEEEECCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCCCCCHHHHH
TTLFPSVILQQQVNSVSTRHIQPDGHGSFDFVWTHFAFEDDDDEMLQRRLRQANLFGPAG
HHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
FVSADDGEVIEFSQEGFEQKPFHRALAELGGRAVDLHTDHMVTETLIRGMYGYWRRVMEE
CCCCCCCCEEEECCCCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
GGGPRGSTPQAGGAEVSQ
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]