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The map label for this gene is nagAa [H]

Identifier: 119898808

GI number: 119898808

Start: 2783542

End: 2784528

Strand: Reverse

Name: nagAa [H]

Synonym: azo2517

Alternate gene names: 119898808

Gene position: 2784528-2783542 (Counterclockwise)

Preceding gene: 119898810

Following gene: 119898807

Centisome position: 63.63

GC content: 69.91

Gene sequence:

>987_bases
ATGGAACTGCATGTACAGCCGATCGACCGCAAGCTGAACTTCGAGATCGGTGCCAACCTGCTGGAGGTGCTGCGCAACGC
CGAGGTGCCGATCTCCTACAGCTGCATGTCCGGTCGCTGCGGCACCTGCCGCTGCGAGGTGCGGGCCGGCGAGGTGCTGG
ACGCCGGCCCGGCAGCCGGGCGCAGCGGGCGCAACGGCCAGAGCGGCGTGCTCGCCTGTCAGGCGGTGCTCACCAGCGAC
TGCGTAATCGAAATCCCCGAGCCGGACGAGGTGGTGGTGCACCGGGCCCGCATCGTCAAGGCCACCGTCGCCGCGATCGA
GGCCCCCACCCACGACATCCGCCGCCTGCGCCTGAAGCTCGCCAAGCCGCTGGACTTCTCGCCCGGCCAGTACGCCAGCC
TGCAATTCACGCCGGAGCACATCCGGCCGTATTCGATGGCCGGGCTGGCGGGCGACGACGAGCAGGAATTCCAGGTGCGG
CGGGTCGAAGGCGGCCGCGTCAGCGCCTGGATCTTCGACCAGTTGAAGGTGGGCGACGCGCTGCGGGTGTCCGGCCCGCT
CGGCACCGCCTACCTGCGCCGCAAGCACGCGGGGCCGATGCTGTGCGTGGGCGGCGGCACCGGGCTGGCGCCGGTGCTCT
CCATCCTGCGCGGCGCGCTCGAATCCGGCATGAAAAACCCCATCCACCTCTACTTCGGCGTGCGCAGCCCGCGCGGCCTC
TACGACGCCGACCGGCTGCAGTCGCTCGCCGACGCATACGACAACCTGACGGTGCACATCGTGGTCGCTACCGGCAACAC
CGATGCATCGCTGCGCAGCGGCCTGGTGACCGACGCGATTGCAGCCGACCACCGCAGTCTTGCCGGCTGGCGCGCCTACC
TGTGCGGCGCGCCAGCGATGGTGGAGGCAGTGGGCTTGCTGGCGAACCGGCTGGGGATTGCGCCGAATCACGTGCATGCG
GATGCGTTTTATCCGGCGGCGTGGTGA

Upstream 100 bases:

>100_bases
TTCAGGTTGGTGATGTGACGTATTCAATCAATTTTATGGATGTATAACACGCCTCGATCCATGATGGCGCCCATCGAACG
TTTCATCACCGGGGCCAGCA

Downstream 100 bases:

>100_bases
GGGTGGGTTTTAGTGATTGGACGACAGGATTAGGTGACGCGTGCGCTCGGAGCGAGGGCTTTTGCTCCCTCCCCTTCGAG
GGGGTGAGGACGGGGTTTCG

Product: naphthalene 1,2-dioxygenase/ferredoxin--NAD(+) reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MELHVQPIDRKLNFEIGANLLEVLRNAEVPISYSCMSGRCGTCRCEVRAGEVLDAGPAAGRSGRNGQSGVLACQAVLTSD
CVIEIPEPDEVVVHRARIVKATVAAIEAPTHDIRRLRLKLAKPLDFSPGQYASLQFTPEHIRPYSMAGLAGDDEQEFQVR
RVEGGRVSAWIFDQLKVGDALRVSGPLGTAYLRRKHAGPMLCVGGGTGLAPVLSILRGALESGMKNPIHLYFGVRSPRGL
YDADRLQSLADAYDNLTVHIVVATGNTDASLRSGLVTDAIAADHRSLAGWRAYLCGAPAMVEAVGLLANRLGIAPNHVHA
DAFYPAAW

Sequences:

>Translated_328_residues
MELHVQPIDRKLNFEIGANLLEVLRNAEVPISYSCMSGRCGTCRCEVRAGEVLDAGPAAGRSGRNGQSGVLACQAVLTSD
CVIEIPEPDEVVVHRARIVKATVAAIEAPTHDIRRLRLKLAKPLDFSPGQYASLQFTPEHIRPYSMAGLAGDDEQEFQVR
RVEGGRVSAWIFDQLKVGDALRVSGPLGTAYLRRKHAGPMLCVGGGTGLAPVLSILRGALESGMKNPIHLYFGVRSPRGL
YDADRLQSLADAYDNLTVHIVVATGNTDASLRSGLVTDAIAADHRSLAGWRAYLCGAPAMVEAVGLLANRLGIAPNHVHA
DAFYPAAW
>Mature_328_residues
MELHVQPIDRKLNFEIGANLLEVLRNAEVPISYSCMSGRCGTCRCEVRAGEVLDAGPAAGRSGRNGQSGVLACQAVLTSD
CVIEIPEPDEVVVHRARIVKATVAAIEAPTHDIRRLRLKLAKPLDFSPGQYASLQFTPEHIRPYSMAGLAGDDEQEFQVR
RVEGGRVSAWIFDQLKVGDALRVSGPLGTAYLRRKHAGPMLCVGGGTGLAPVLSILRGALESGMKNPIHLYFGVRSPRGL
YDADRLQSLADAYDNLTVHIVVATGNTDASLRSGLVTDAIAADHRSLAGWRAYLCGAPAMVEAVGLLANRLGIAPNHVHA
DAFYPAAW

Specific function: Component of naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis- naphthalene dihydrodiol. Transfers electrons from ferredoxin (ndoA) to NADH [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=225, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787658, Length=237, Percent_Identity=26.1603375527426, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788903, Length=214, Percent_Identity=23.3644859813084, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 35164; Mature: 35164

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELHVQPIDRKLNFEIGANLLEVLRNAEVPISYSCMSGRCGTCRCEVRAGEVLDAGPAAG
CCEECCCCCCEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEECCCCCCC
RSGRNGQSGVLACQAVLTSDCVIEIPEPDEVVVHRARIVKATVAAIEAPTHDIRRLRLKL
CCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AKPLDFSPGQYASLQFTPEHIRPYSMAGLAGDDEQEFQVRRVEGGRVSAWIFDQLKVGDA
CCCCCCCCCCEEEEEECHHHCCCEEECCCCCCCHHHEEEEEECCCEEEEEEEEHHCCCCE
LRVSGPLGTAYLRRKHAGPMLCVGGGTGLAPVLSILRGALESGMKNPIHLYFGVRSPRGL
EEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
YDADRLQSLADAYDNLTVHIVVATGNTDASLRSGLVTDAIAADHRSLAGWRAYLCGAPAM
CCHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHH
VEAVGLLANRLGIAPNHVHADAFYPAAW
HHHHHHHHHHHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MELHVQPIDRKLNFEIGANLLEVLRNAEVPISYSCMSGRCGTCRCEVRAGEVLDAGPAAG
CCEECCCCCCEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEECCCCCCC
RSGRNGQSGVLACQAVLTSDCVIEIPEPDEVVVHRARIVKATVAAIEAPTHDIRRLRLKL
CCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AKPLDFSPGQYASLQFTPEHIRPYSMAGLAGDDEQEFQVRRVEGGRVSAWIFDQLKVGDA
CCCCCCCCCCEEEEEECHHHCCCEEECCCCCCCHHHEEEEEECCCEEEEEEEEHHCCCCE
LRVSGPLGTAYLRRKHAGPMLCVGGGTGLAPVLSILRGALESGMKNPIHLYFGVRSPRGL
EEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
YDADRLQSLADAYDNLTVHIVVATGNTDASLRSGLVTDAIAADHRSLAGWRAYLCGAPAM
CCHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHH
VEAVGLLANRLGIAPNHVHADAFYPAAW
HHHHHHHHHHHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486285; 8787402; 2294092 [H]