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The map label for this gene is paaJ2 [H]

Identifier: 119898788

GI number: 119898788

Start: 2760328

End: 2761587

Strand: Reverse

Name: paaJ2 [H]

Synonym: azo2497

Alternate gene names: 119898788

Gene position: 2761587-2760328 (Counterclockwise)

Preceding gene: 119898789

Following gene: 119898787

Centisome position: 63.11

GC content: 69.92

Gene sequence:

>1260_bases
ATGAGCAATCCGTACTCCGCCTCCGAAGCCGCCGCGCTGACGACGTCCTACGACGACATCTGGCTGGTGGCCGGCCAGCG
CACCCCGTTCGCCGACTACAACGGCGTGCTGCGCGACGTCTCGCCCACCGACCTCGGCATCTACGCCGCGCGCGCGCTGT
TCGAGAAGAGTGGCATCCCGGCTTCCGCCATCCAGGCCATCGTCGCCGGCAACATGGCGCAGGCGAGTTTCGACGCCTAC
TTCATGCCGCGCCAGATCGGTCTGTATTCCGGCGTCAATCCCAACGTGCCGGCGGTGCTGGTGCAGCGCCTGTGCGGCAC
CGGCTTCGAGGCCATCCTGACCGCCGCCGACCAGATCAAGCTCGGCAAGGCCAAGGTGGCGCTGGCGGTCGGCACCGAGT
CGATGTCGCGCAATCCGGTGGCCGCCTACACCCACCGCGCCGGCTTCCGCATGGGCCAGGTGGATTTCCGCGACTTCCTG
TGGGAAGCGACCAAGGACACGTCCTTCGGCGGCAGCATGGGTGACACCGCCGAGAACCTCGCCAAGCGCTACGGCATCAG
CCGCGAGGAAGTCGACCGCTTCGCCGAGCTGAGCTTCTCGCGCGCGGTGGCGGGCTGGGAATCCGGCTACTTCGACGACG
AAGTGAGCGCGGTGGTGAATAGCAAGTGGGATCTGGAAGGCTACAACAGCCGCGGCCTCAAGCTTGCCGACCGTGCCGAG
CGCTGCGAGCGCGACGGCCATGTGCGCGCCACCTCTTTCGAAACCCTGCAGAAGCTCAAACCCGCCTTCGGCGGCGTGCA
GACCGGCGGCAACAGCTCGGCCATCGTCGATGGCGCGGCGGCGGTGATCGTCGCCCACGGCGACTGGGTGCGGGCGAACG
GCGTCAAGCCGCTGGCGCGCATCGTCGCCGGCGCCGCGGTCGCGGTGCCGCCGGAAATCATGGGCATCGGCCCGGCCCCG
GCCATCCGCGCTGCGGCGAGGACCGCCGGCCTCACCGTCGGCGACCTCGGCCGCATCGAGATCAATGAAGCCTTCGGCGC
CCAGTACCTCGCGTGCCAGCGCGAACTGGGTTTCGACACCGAGCGCGGCAATGTCCACGGCGGCGCAATCGCCATCGGCC
ACCCGCTGGGGGCCTCGGGCGTGCGTCTGACCACGACCGTGGCGCGCGAGCTGAAGGAAGCCGGGGCGCAGTTCGGCGTG
TCGTCGGCCTGCGCCGGCGGCGGGCAGGGCGTGGCGATCGTGGTGGAGAACGCGGGCTGA

Upstream 100 bases:

>100_bases
TCTTCGTCGAATCGCTGACCGCCGCACTGACCCAGACCAGCCCCGAAGTCGCCGAGCGTATCGGCCACTTCCTCACCCGC
AAAGGCAAGGGACCGCAAAA

Downstream 100 bases:

>100_bases
ATCGGGGCGCAGACGGGCAGGGGGGGACCGCTCCCTCCCCTTCAAGGGGAGGGCTGGGGTGGGGATGGGGTTAGCAGGCG
TGGCAGAACCCCATCCCCCT

Product: putative beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSNPYSASEAAALTTSYDDIWLVAGQRTPFADYNGVLRDVSPTDLGIYAARALFEKSGIPASAIQAIVAGNMAQASFDAY
FMPRQIGLYSGVNPNVPAVLVQRLCGTGFEAILTAADQIKLGKAKVALAVGTESMSRNPVAAYTHRAGFRMGQVDFRDFL
WEATKDTSFGGSMGDTAENLAKRYGISREEVDRFAELSFSRAVAGWESGYFDDEVSAVVNSKWDLEGYNSRGLKLADRAE
RCERDGHVRATSFETLQKLKPAFGGVQTGGNSSAIVDGAAAVIVAHGDWVRANGVKPLARIVAGAAVAVPPEIMGIGPAP
AIRAAARTAGLTVGDLGRIEINEAFGAQYLACQRELGFDTERGNVHGGAIAIGHPLGASGVRLTTTVARELKEAGAQFGV
SSACAGGGQGVAIVVENAG

Sequences:

>Translated_419_residues
MSNPYSASEAAALTTSYDDIWLVAGQRTPFADYNGVLRDVSPTDLGIYAARALFEKSGIPASAIQAIVAGNMAQASFDAY
FMPRQIGLYSGVNPNVPAVLVQRLCGTGFEAILTAADQIKLGKAKVALAVGTESMSRNPVAAYTHRAGFRMGQVDFRDFL
WEATKDTSFGGSMGDTAENLAKRYGISREEVDRFAELSFSRAVAGWESGYFDDEVSAVVNSKWDLEGYNSRGLKLADRAE
RCERDGHVRATSFETLQKLKPAFGGVQTGGNSSAIVDGAAAVIVAHGDWVRANGVKPLARIVAGAAVAVPPEIMGIGPAP
AIRAAARTAGLTVGDLGRIEINEAFGAQYLACQRELGFDTERGNVHGGAIAIGHPLGASGVRLTTTVARELKEAGAQFGV
SSACAGGGQGVAIVVENAG
>Mature_418_residues
SNPYSASEAAALTTSYDDIWLVAGQRTPFADYNGVLRDVSPTDLGIYAARALFEKSGIPASAIQAIVAGNMAQASFDAYF
MPRQIGLYSGVNPNVPAVLVQRLCGTGFEAILTAADQIKLGKAKVALAVGTESMSRNPVAAYTHRAGFRMGQVDFRDFLW
EATKDTSFGGSMGDTAENLAKRYGISREEVDRFAELSFSRAVAGWESGYFDDEVSAVVNSKWDLEGYNSRGLKLADRAER
CERDGHVRATSFETLQKLKPAFGGVQTGGNSSAIVDGAAAVIVAHGDWVRANGVKPLARIVAGAAVAVPPEIMGIGPAPA
IRAAARTAGLTVGDLGRIEINEAFGAQYLACQRELGFDTERGNVHGGAIAIGHPLGASGVRLTTTVARELKEAGAQFGVS
SACAGGGQGVAIVVENAG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=402, Percent_Identity=40.547263681592, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI148539872, Length=404, Percent_Identity=36.1386138613861, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=36.0975609756098, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI4557237, Length=406, Percent_Identity=31.7733990147783, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI4504327, Length=439, Percent_Identity=30.0683371298405, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194353979, Length=93, Percent_Identity=48.3870967741936, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788554, Length=407, Percent_Identity=38.3292383292383, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082165, Length=406, Percent_Identity=37.6847290640394, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1787663, Length=408, Percent_Identity=36.7647058823529, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI48994986, Length=410, Percent_Identity=36.8292682926829, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788683, Length=446, Percent_Identity=33.4080717488789, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.3915211970075, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=33.1695331695332, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147385, Length=418, Percent_Identity=33.2535885167464, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=440, Percent_Identity=30.2272727272727, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=415, Percent_Identity=28.433734939759, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=419, Percent_Identity=33.890214797136, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=31.8518518518519, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17648125, Length=409, Percent_Identity=41.0757946210269, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24655093, Length=404, Percent_Identity=35.1485148514851, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=33.1683168316832, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI17137578, Length=436, Percent_Identity=31.1926605504587, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 43814; Mature: 43682

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPYSASEAAALTTSYDDIWLVAGQRTPFADYNGVLRDVSPTDLGIYAARALFEKSGIP
CCCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ASAIQAIVAGNMAQASFDAYFMPRQIGLYSGVNPNVPAVLVQRLCGTGFEAILTAADQIK
HHHHHHHHHCCHHHHCCCEEECCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEE
LGKAKVALAVGTESMSRNPVAAYTHRAGFRMGQVDFRDFLWEATKDTSFGGSMGDTAENL
ECCEEEEEEECCCCCCCCCCHHHHHHCCCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHH
AKRYGISREEVDRFAELSFSRAVAGWESGYFDDEVSAVVNSKWDLEGYNSRGLKLADRAE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHH
RCERDGHVRATSFETLQKLKPAFGGVQTGGNSSAIVDGAAAVIVAHGDWVRANGVKPLAR
HHHCCCCEEECHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCEEECCCCCHHHH
IVAGAAVAVPPEIMGIGPAPAIRAAARTAGLTVGDLGRIEINEAFGAQYLACQRELGFDT
HHHCCCEECCHHHCCCCCCHHHHHHHHHCCCEECCCCEEEECHHHCHHHHHHHHHHCCCC
ERGNVHGGAIAIGHPLGASGVRLTTTVARELKEAGAQFGVSSACAGGGQGVAIVVENAG
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure 
SNPYSASEAAALTTSYDDIWLVAGQRTPFADYNGVLRDVSPTDLGIYAARALFEKSGIP
CCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ASAIQAIVAGNMAQASFDAYFMPRQIGLYSGVNPNVPAVLVQRLCGTGFEAILTAADQIK
HHHHHHHHHCCHHHHCCCEEECCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEE
LGKAKVALAVGTESMSRNPVAAYTHRAGFRMGQVDFRDFLWEATKDTSFGGSMGDTAENL
ECCEEEEEEECCCCCCCCCCHHHHHHCCCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHH
AKRYGISREEVDRFAELSFSRAVAGWESGYFDDEVSAVVNSKWDLEGYNSRGLKLADRAE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHH
RCERDGHVRATSFETLQKLKPAFGGVQTGGNSSAIVDGAAAVIVAHGDWVRANGVKPLAR
HHHCCCCEEECHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCEEECCCCCHHHH
IVAGAAVAVPPEIMGIGPAPAIRAAARTAGLTVGDLGRIEINEAFGAQYLACQRELGFDT
HHHCCCEECCHHHCCCCCCHHHHHHHHHCCCEECCCCEEEECHHHCHHHHHHHHHHCCCC
ERGNVHGGAIAIGHPLGASGVRLTTTVARELKEAGAQFGVSSACAGGGQGVAIVVENAG
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]