BacMap

Definition	Azoarcus sp. BH72 chromosome, complete genome.
Accession	NC_008702
Length	4,376,040

Click here to switch to the map view.

The map label for this gene is fabG2 [H]

Identifier: 119898787

GI number: 119898787

Start: 2759364

End: 2760131

Strand: Reverse

Name: fabG2 [H]

Synonym: azo2496

Alternate gene names: 119898787

Gene position: 2760131-2759364 (Counterclockwise)

Preceding gene: 119898788

Following gene: 119898786

Centisome position: 63.07

GC content: 68.49

Gene sequence:

>768_bases
ATGAAATTCGACAACAACACCTTCATCGTCACCGGCGGTGCCTCCGGCCTGGGCGAAGCCACCGTGCGTGCCTTCCACGC
CCAGGGCGCCAAGGTCGTGATCGCCGATCTCAATGTCGACCAGGGCGAAAAGCTCGCCGCCGAACTCGGCGAGCGTACCG
TCTTCTGCCGCACCAACGTCGCCGACGAGGCCGATGCCAAGGCGGCGGTCGACACCGCGCTGACGCGCTTCGGCGGCCTG
CAGGGGCTGGTGAACTGCGCCGGTGTCGGCACCGCCGGCAAGGTGCTGCCCAAGGACGGCGTGATGCCGCTGGGCGACTT
CGCGCGCTGCATCCAGATCAACCTGATCGGCTCCTTCAACATGATCCGGCTGGCGTCGGAAGCCATGAGCAAGGGCGAGC
CGCAGGCCGACGGCGAGCGCGGCGTCATCATCAACACCGCCTCGGTCGCCGCCTACGACGGCCAGATCGGCCAGGCGGCG
TATTCGGCGTCGAAGGGCGGCATCGTCTCGATGACGCTGCCGATCGCGCGCGAACTGGCGCGCTTCGGCATCCGCGTGAT
GACCATCGCCCCGGGCCTCTTCATCACCCCGATGATGCGCGGCCTGCCGCCGGAAGTGCAGAAGTCGCTCGGCGAAGCCA
CGCCCTTCCCGCAGCGCTTGGGCGAGCCGGCCGAGTTCGCCAAGCTCGCCGTCAGCATCGTCGACAACGTCATGCTCAAC
GGCGAAACCATCCGCCTCGACGGCGCCGTGCGCCTCGCGCCCCGCTAA

Upstream 100 bases:

>100_bases
CCCTCCTAGCCTCCCCCTTGAAGGGGGAGGGACAGAACCATCCCGGCTGCAAAGTCAGATATTCAATTGCCGCCTTGACG
GGTCGGCAGGAAGGAACTTC

Downstream 100 bases:

>100_bases
CCCACACACGACACGACCATGGCCCGCAGCAACATCTACCGCATGCGCATCGCCTTCTCCGATTGCGACCCGGCGGAAAT
CGTCTTCTTCGCCAACTACT

Product: short-chain dehydrogenase family protein

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKFDNNTFIVTGGASGLGEATVRAFHAQGAKVVIADLNVDQGEKLAAELGERTVFCRTNVADEADAKAAVDTALTRFGGL
QGLVNCAGVGTAGKVLPKDGVMPLGDFARCIQINLIGSFNMIRLASEAMSKGEPQADGERGVIINTASVAAYDGQIGQAA
YSASKGGIVSMTLPIARELARFGIRVMTIAPGLFITPMMRGLPPEVQKSLGEATPFPQRLGEPAEFAKLAVSIVDNVMLN
GETIRLDGAVRLAPR

Sequences:

>Translated_255_residues
MKFDNNTFIVTGGASGLGEATVRAFHAQGAKVVIADLNVDQGEKLAAELGERTVFCRTNVADEADAKAAVDTALTRFGGL
QGLVNCAGVGTAGKVLPKDGVMPLGDFARCIQINLIGSFNMIRLASEAMSKGEPQADGERGVIINTASVAAYDGQIGQAA
YSASKGGIVSMTLPIARELARFGIRVMTIAPGLFITPMMRGLPPEVQKSLGEATPFPQRLGEPAEFAKLAVSIVDNVMLN
GETIRLDGAVRLAPR
>Mature_255_residues
MKFDNNTFIVTGGASGLGEATVRAFHAQGAKVVIADLNVDQGEKLAAELGERTVFCRTNVADEADAKAAVDTALTRFGGL
QGLVNCAGVGTAGKVLPKDGVMPLGDFARCIQINLIGSFNMIRLASEAMSKGEPQADGERGVIINTASVAAYDGQIGQAA
YSASKGGIVSMTLPIARELARFGIRVMTIAPGLFITPMMRGLPPEVQKSLGEATPFPQRLGEPAEFAKLAVSIVDNVMLN
GETIRLDGAVRLAPR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=248, Percent_Identity=54.0322580645161, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI83715985, Length=248, Percent_Identity=50.4032258064516, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=29.6747967479675, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542939, Length=262, Percent_Identity=28.2442748091603, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI59889578, Length=216, Percent_Identity=31.9444444444444, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI10190704, Length=253, Percent_Identity=29.2490118577075, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI40548397, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI214010158, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI214010156, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI22758144, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4504505, Length=202, Percent_Identity=32.1782178217822, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI22507405, Length=237, Percent_Identity=27.4261603375527, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI5031737, Length=261, Percent_Identity=29.1187739463602, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI150247226, Length=230, Percent_Identity=29.1304347826087, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787905, Length=235, Percent_Identity=30.6382978723404, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082100, Length=240, Percent_Identity=31.25, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=28.740157480315, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=30.241935483871, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789378, Length=242, Percent_Identity=28.5123966942149, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789057, Length=217, Percent_Identity=31.3364055299539, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786812, Length=268, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=28.4584980237154, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=29.718875502008, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=26.2948207171315, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=27.8884462151394, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=246, Percent_Identity=46.3414634146341, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=29.718875502008, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=246, Percent_Identity=30.4878048780488, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17563726, Length=274, Percent_Identity=26.6423357664234, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=262, Percent_Identity=30.5343511450382, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=274, Percent_Identity=25.5474452554745, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=25.830258302583, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=271, Percent_Identity=26.5682656826568, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=270, Percent_Identity=25.1851851851852, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=27.5590551181102, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=24.4094488188976, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=27.037037037037, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=25.1851851851852, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=203, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=274, Percent_Identity=26.2773722627737, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32563809, Length=226, Percent_Identity=26.9911504424779, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=226, Percent_Identity=26.9911504424779, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=25.6916996047431, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=270, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=275, Percent_Identity=26.5454545454545, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=245, Percent_Identity=28.9795918367347, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17737361, Length=252, Percent_Identity=54.7619047619048, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24639444, Length=247, Percent_Identity=32.7935222672065, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24644339, Length=239, Percent_Identity=28.8702928870293, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=237, Percent_Identity=27.8481012658228, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571526, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665243, Length=211, Percent_Identity=28.9099526066351, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221331218, Length=211, Percent_Identity=28.9099526066351, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665247, Length=212, Percent_Identity=29.2452830188679, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221331216, Length=212, Percent_Identity=29.2452830188679, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571387, Length=222, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI23397609, Length=203, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355319, Length=243, Percent_Identity=28.3950617283951, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26618; Mature: 26618

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDNNTFIVTGGASGLGEATVRAFHAQGAKVVIADLNVDQGEKLAAELGERTVFCRTNV
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCEEEEECCC
ADEADAKAAVDTALTRFGGLQGLVNCAGVGTAGKVLPKDGVMPLGDFARCIQINLIGSFN
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEEEEECCCH
MIRLASEAMSKGEPQADGERGVIINTASVAAYDGQIGQAAYSASKGGIVSMTLPIARELA
HHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEEHHHHHHHH
RFGIRVMTIAPGLFITPMMRGLPPEVQKSLGEATPFPQRLGEPAEFAKLAVSIVDNVMLN
HHCEEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHEEC
GETIRLDGAVRLAPR
CCEEEECCEEEECCC
>Mature Secondary Structure
MKFDNNTFIVTGGASGLGEATVRAFHAQGAKVVIADLNVDQGEKLAAELGERTVFCRTNV
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCEEEEECCC
ADEADAKAAVDTALTRFGGLQGLVNCAGVGTAGKVLPKDGVMPLGDFARCIQINLIGSFN
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEEEEECCCH
MIRLASEAMSKGEPQADGERGVIINTASVAAYDGQIGQAAYSASKGGIVSMTLPIARELA
HHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEEHHHHHHHH
RFGIRVMTIAPGLFITPMMRGLPPEVQKSLGEATPFPQRLGEPAEFAKLAVSIVDNVMLN
HHCEEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHEEC
GETIRLDGAVRLAPR
CCEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]