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The map label for this gene is nahL [H]

Identifier: 119898725

GI number: 119898725

Start: 2686737

End: 2687519

Strand: Reverse

Name: nahL [H]

Synonym: azo2434

Alternate gene names: 119898725

Gene position: 2687519-2686737 (Counterclockwise)

Preceding gene: 119898726

Following gene: 119898724

Centisome position: 61.41

GC content: 66.41

Gene sequence:

>783_bases
ATGGACAAGACCTTGATCGAACGGCTCGGCGACGAGCTTTACCAGGCGCTGGTCAAGCGCGAAGTGCTGGCGCCGCTGAC
CGAACGCCATCCGGACATCACCATCGCCGACGCCTACCGCATCCAGCAGCGCATGAACGCGCGCCGGCTGGAGGCGGGCG
AGCGGGTGGTGGGCAAGAAGATCGGCGTCACCTCGAAGGCGGTGATGAACATGCTCGGCGTCTACCAGCCGGACTTCGGC
ATCCTCACCGACGGCATGCTCTACAACGAGGGCGAGGCCATCGCCGCCGACACCCTGATCCAGCCCAAGGCCGAAGGCGA
GATCGCCTTCGTGCTGAAGAAGGATCTGAAGGGCCCCGGCGTGACCGCCGCCGACGTGCTGGCCGCCACCGAAGGCGTGA
TGACCTGCTTCGAGATCGTCGATTCGCGCATCCGCGACTGGAAAATCAAGATCCAGGACACCGTCGCCGACAACGCCTCC
TGCGGCGTCTTCGTGCTGGGCGACAGCCTGGTCGATCCGCGCAAGGTCGATCTGAACACCTGCGGGATGATCCTCGAGAA
GAACGGCGAGATCGTCGCCACCGGTTCTGGCGCCGCGGCGCTGGGCGCGCCGGCCAACGCGGTCGCCTGGCTGGCCAACA
CGCTGGGCAGCCTGGACATTCCGTTGCAGGCGGGCGAAGTCATCCTGTCCGGTTCGCTCGCGATCATGGTGCCGGTGAAG
GCAGGCGACAACCTGCGCGTCACCATCGGCGGCATCGGCGGCTGCTCGGTGCGCTTCGTCTGA

Upstream 100 bases:

>100_bases
TTCGCGGCGCACGCGCCGCGCCTGTCGAGCGGGCCGGCTGTGCAAAGCCGGCACTCCACGGGTTACCCCTCTTCGCGCAG
CGACAAACTGGAGGTAGCAA

Downstream 100 bases:

>100_bases
TTTCCCGCTTGCAGGCGCGCGGCTGGGCGAATCCTCTCCCTCTCTCCCGCCCCCGTCGCGTGGCCTCTTCCTTTCTCCCG
ACAGGAGACAACACATGGAT

Product: 2-oxopent-4-enoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDKTLIERLGDELYQALVKREVLAPLTERHPDITIADAYRIQQRMNARRLEAGERVVGKKIGVTSKAVMNMLGVYQPDFG
ILTDGMLYNEGEAIAADTLIQPKAEGEIAFVLKKDLKGPGVTAADVLAATEGVMTCFEIVDSRIRDWKIKIQDTVADNAS
CGVFVLGDSLVDPRKVDLNTCGMILEKNGEIVATGSGAAALGAPANAVAWLANTLGSLDIPLQAGEVILSGSLAIMVPVK
AGDNLRVTIGGIGGCSVRFV

Sequences:

>Translated_260_residues
MDKTLIERLGDELYQALVKREVLAPLTERHPDITIADAYRIQQRMNARRLEAGERVVGKKIGVTSKAVMNMLGVYQPDFG
ILTDGMLYNEGEAIAADTLIQPKAEGEIAFVLKKDLKGPGVTAADVLAATEGVMTCFEIVDSRIRDWKIKIQDTVADNAS
CGVFVLGDSLVDPRKVDLNTCGMILEKNGEIVATGSGAAALGAPANAVAWLANTLGSLDIPLQAGEVILSGSLAIMVPVK
AGDNLRVTIGGIGGCSVRFV
>Mature_260_residues
MDKTLIERLGDELYQALVKREVLAPLTERHPDITIADAYRIQQRMNARRLEAGERVVGKKIGVTSKAVMNMLGVYQPDFG
ILTDGMLYNEGEAIAADTLIQPKAEGEIAFVLKKDLKGPGVTAADVLAATEGVMTCFEIVDSRIRDWKIKIQDTVADNAS
CGVFVLGDSLVDPRKVDLNTCGMILEKNGEIVATGSGAAALGAPANAVAWLANTLGSLDIPLQAGEVILSGSLAIMVPVK
AGDNLRVTIGGIGGCSVRFV

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=259, Percent_Identity=42.4710424710425, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 27565; Mature: 27565

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTLIERLGDELYQALVKREVLAPLTERHPDITIADAYRIQQRMNARRLEAGERVVGKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
IGVTSKAVMNMLGVYQPDFGILTDGMLYNEGEAIAADTLIQPKAEGEIAFVLKKDLKGPG
CCCHHHHHHHHHHCCCCCCCEEECCEEECCCCEEEECCEECCCCCCCEEEEEEECCCCCC
VTAADVLAATEGVMTCFEIVDSRIRDWKIKIQDTVADNASCGVFVLGDSLVDPRKVDLNT
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCCCCCCEECHHH
CGMILEKNGEIVATGSGAAALGAPANAVAWLANTLGSLDIPLQAGEVILSGSLAIMVPVK
CCEEEECCCCEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEEE
AGDNLRVTIGGIGGCSVRFV
CCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure
MDKTLIERLGDELYQALVKREVLAPLTERHPDITIADAYRIQQRMNARRLEAGERVVGKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
IGVTSKAVMNMLGVYQPDFGILTDGMLYNEGEAIAADTLIQPKAEGEIAFVLKKDLKGPG
CCCHHHHHHHHHHCCCCCCCEEECCEEECCCCEEEECCEECCCCCCCEEEEEEECCCCCC
VTAADVLAATEGVMTCFEIVDSRIRDWKIKIQDTVADNASCGVFVLGDSLVDPRKVDLNT
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCCCCCCEECHHH
CGMILEKNGEIVATGSGAAALGAPANAVAWLANTLGSLDIPLQAGEVILSGSLAIMVPVK
CCEEEECCCCEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEEE
AGDNLRVTIGGIGGCSVRFV
CCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]