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The map label for this gene is fumB [H]

Identifier: 119898706

GI number: 119898706

Start: 2668208

End: 2669899

Strand: Direct

Name: fumB [H]

Synonym: azo2415

Alternate gene names: 119898706

Gene position: 2668208-2669899 (Clockwise)

Preceding gene: 119898699

Following gene: 119898709

Centisome position: 60.97

GC content: 65.13

Gene sequence:

>1692_bases
ATGACCGTGATTCGCGAAGAAGACCTCATCCAGTCCATCGCGGATGCCTTCCAGTACATCAGCTACTACCATCCGCTCGA
CTACATCAAGGCGCTCGGTGAAGCGTACGAACGCGAGGAAAGCCCCGCCGCGAAGGACGCGATTGCGCAGATCCTCACCA
ATTCACGCATGTGCGCCGAAGGCCACCGCCCCATCTGCCAGGACACCGGCATCGCCGTGGTCTTCCTCAAGGTCGGCATG
AACGTGCAGTGGGACGCCAAGATGAGCGTCCAGGAGATGGTCAACGAAGGCGTGCGCCGCGCCTACAACCACCCGGACAA
CAAGCTGCGCGCCTCGGTGCTGCTCGATCCCGCCGGCGCGCGCAAGAACTCCAAGGACAACACGCCGGCCGTTGTGCACT
ACGAGATCGTGCCGGGCGATCACGTCGAGATCACCTGTGCGGCCAAGGGCGGCGGCTCGGAAAACAAGTCGAAGATGGTC
ATGCTCAACCCGTCCGATTCCATCGTCGACTGGGTGCTGAAGACCGTGCCGACCATGGGCGCCGGCTGGTGTCCGCCTGG
CATCCTCGGCATCGGCATCGGCGGCACGCCGGAAAAGGCGATGCTGCTGGCCAAGGAATCGCTGATGGCGCCGGTGGACA
TCCATGAGCTGAAGGAAAAGGCCGCCTCCGGCGCCGCGCTGTCGCGCGTCGAGGAACTGCGCCTCGAACTGATGGAAAAG
GTGAACGCGCTCGGCATCGGCGCCCAGGGCCTCGGCGGCCTCACCACCGTGCTCGACGTCAAGATCATGGACTACCCGAC
CCACGCGGCCTCGCTGCCGGTGGCGATGATCCCGAACTGCGCGGCCACCCGCCACGTGCATTTCGAACTCGACGGCTCCG
GCCCGGCCAAGCTCGAAACGCCCAAGCTCGAAGACTGGCCCGCCGTGACCTGGAAGGCCGACACCAACGTCGCCACCCGG
GTGAATCTCGACACCCTGACCAAGGAAGAAGTCGCGTCCTGGAAGCCGGGCCAGATCCTGCTGCTCAACGGCAAGATGCT
CACCGGCCGCGACGCCGCGCACAAGCGCATCCAGGACATGCTGGCCAAGGGCGAGAAGCTGCCGGTCGATTTCACCAACC
GCGTCATCTACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAAGTCGTCGGCCCGGCCGGCCCCACCACCGCCACCCGC
ATGGACAAGTTCACCCGCATGATGCTGGAGCAGACCGGCCTCATCTCGATGATCGGCAAGTCCGAGCGCGGCCCGGTCGC
GATCGATGCGATCCGCGACAACAAGTCCGCCTACCTGATGGCGGTCGGCGGCTCCGCCTACCTCGTCTCCAAGGCGATCA
AGGAAGCCAAGGTCGTCGGCTTTGCGGACCTCGGCATGGAGGCGATCTACGAATTCACCGTGCAGGACATGCCGGTGACC
GTGGCGGTCGATTCCACCGGCACCAGCGTGCACAACACCGGCCCGAAGGAATGGCAGGCGAAGATCGGCAAGATCCCGGT
CGCCGTGCCTGAGCATCGGCAGACAGCGGCAAACAAAAAGCCCGGCGGGGATTTCCCCGCCGGGCTTTTTTCTTCGCACT
ACCACTGCCGGTCGCTACACCGACCGCCGCCGCGACGGCCGTCGCGGCAGGAGATGTTCAGTGGTAGCGCGGCATACAGG
ACATGCCGGTGA

Upstream 100 bases:

>100_bases
GCTTCGCTGCAGCAAAAATCCGGCATTCCAGCCCCCTGGCACCGCCCGCCTGCAACCGAAGCCCACCCATCACCCGTTCC
TGCACCGGAGACCTTGCGCC

Downstream 100 bases:

>100_bases
ATTCGAACGGGAACGCTTCGGGCTTGCCTTCGACGGGTTCGAGGTCCCACAGATACTTGCCACCCAACACCTGCAGGCGG
CGCAGCGCCTGCGAGGACAG

Product: putative fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTVIREEDLIQSIADAFQYISYYHPLDYIKALGEAYEREESPAAKDAIAQILTNSRMCAEGHRPICQDTGIAVVFLKVGM
NVQWDAKMSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGARKNSKDNTPAVVHYEIVPGDHVEITCAAKGGGSENKSKMV
MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELKEKAASGAALSRVEELRLELMEK
VNALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPNCAATRHVHFELDGSGPAKLETPKLEDWPAVTWKADTNVATR
VNLDTLTKEEVASWKPGQILLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATR
MDKFTRMMLEQTGLISMIGKSERGPVAIDAIRDNKSAYLMAVGGSAYLVSKAIKEAKVVGFADLGMEAIYEFTVQDMPVT
VAVDSTGTSVHNTGPKEWQAKIGKIPVAVPEHRQTAANKKPGGDFPAGLFSSHYHCRSLHRPPPRRPSRQEMFSGSAAYR
TCR

Sequences:

>Translated_563_residues
MTVIREEDLIQSIADAFQYISYYHPLDYIKALGEAYEREESPAAKDAIAQILTNSRMCAEGHRPICQDTGIAVVFLKVGM
NVQWDAKMSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGARKNSKDNTPAVVHYEIVPGDHVEITCAAKGGGSENKSKMV
MLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELKEKAASGAALSRVEELRLELMEK
VNALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPNCAATRHVHFELDGSGPAKLETPKLEDWPAVTWKADTNVATR
VNLDTLTKEEVASWKPGQILLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATR
MDKFTRMMLEQTGLISMIGKSERGPVAIDAIRDNKSAYLMAVGGSAYLVSKAIKEAKVVGFADLGMEAIYEFTVQDMPVT
VAVDSTGTSVHNTGPKEWQAKIGKIPVAVPEHRQTAANKKPGGDFPAGLFSSHYHCRSLHRPPPRRPSRQEMFSGSAAYR
TCR
>Mature_562_residues
TVIREEDLIQSIADAFQYISYYHPLDYIKALGEAYEREESPAAKDAIAQILTNSRMCAEGHRPICQDTGIAVVFLKVGMN
VQWDAKMSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGARKNSKDNTPAVVHYEIVPGDHVEITCAAKGGGSENKSKMVM
LNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELKEKAASGAALSRVEELRLELMEKV
NALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPNCAATRHVHFELDGSGPAKLETPKLEDWPAVTWKADTNVATRV
NLDTLTKEEVASWKPGQILLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRM
DKFTRMMLEQTGLISMIGKSERGPVAIDAIRDNKSAYLMAVGGSAYLVSKAIKEAKVVGFADLGMEAIYEFTVQDMPVTV
AVDSTGTSVHNTGPKEWQAKIGKIPVAVPEHRQTAANKKPGGDFPAGLFSSHYHCRSLHRPPPRRPSRQEMFSGSAAYRT
CR

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=465, Percent_Identity=30.9677419354839, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1787897, Length=464, Percent_Identity=31.8965517241379, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789442, Length=290, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789443, Length=191, Percent_Identity=27.7486910994764, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 61128; Mature: 60997

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50835 IG_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIREEDLIQSIADAFQYISYYHPLDYIKALGEAYEREESPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHC
GHRPICQDTGIAVVFLKVGMNVQWDAKMSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGA
CCCCCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
RKNSKDNTPAVVHYEIVPGDHVEITCAAKGGGSENKSKMVMLNPSDSIVDWVLKTVPTMG
CCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
AGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELKEKAASGAALSRVEELRLELMEK
CCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPNCAATRHVHFELDGSGPAKLET
HHHCCCCCCCCCCEEEEEEEEEEECCCHHHCCCHHCCCCCCCCEEEEEEECCCCCCCCCC
PKLEDWPAVTWKADTNVATRVNLDTLTKEEVASWKPGQILLLNGKMLTGRDAAHKRIQDM
CCCCCCCCEEEECCCCEEEEEEHHHCCHHHHHCCCCCEEEEECCEEECCCHHHHHHHHHH
LAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRMMLEQTGLISMIGK
HHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHEECCC
SERGPVAIDAIRDNKSAYLMAVGGSAYLVSKAIKEAKVVGFADLGMEAIYEFTVQDMPVT
CCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCEE
VAVDSTGTSVHNTGPKEWQAKIGKIPVAVPEHRQTAANKKPGGDFPAGLFSSHYHCRSLH
EEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHCC
RPPPRRPSRQEMFSGSAAYRTCR
CCCCCCCCHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TVIREEDLIQSIADAFQYISYYHPLDYIKALGEAYEREESPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHC
GHRPICQDTGIAVVFLKVGMNVQWDAKMSVQEMVNEGVRRAYNHPDNKLRASVLLDPAGA
CCCCCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
RKNSKDNTPAVVHYEIVPGDHVEITCAAKGGGSENKSKMVMLNPSDSIVDWVLKTVPTMG
CCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
AGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELKEKAASGAALSRVEELRLELMEK
CCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPNCAATRHVHFELDGSGPAKLET
HHHCCCCCCCCCCEEEEEEEEEEECCCHHHCCCHHCCCCCCCCEEEEEEECCCCCCCCCC
PKLEDWPAVTWKADTNVATRVNLDTLTKEEVASWKPGQILLLNGKMLTGRDAAHKRIQDM
CCCCCCCCEEEECCCCEEEEEEHHHCCHHHHHCCCCCEEEEECCEEECCCHHHHHHHHHH
LAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRMMLEQTGLISMIGK
HHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHEECCC
SERGPVAIDAIRDNKSAYLMAVGGSAYLVSKAIKEAKVVGFADLGMEAIYEFTVQDMPVT
CCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCEE
VAVDSTGTSVHNTGPKEWQAKIGKIPVAVPEHRQTAANKKPGGDFPAGLFSSHYHCRSLH
EEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHCC
RPPPRRPSRQEMFSGSAAYRTCR
CCCCCCCCHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]