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The map label for this gene is btuB [H]

Identifier: 119898691

GI number: 119898691

Start: 2640828

End: 2643044

Strand: Direct

Name: btuB [H]

Synonym: azo2400

Alternate gene names: 119898691

Gene position: 2640828-2643044 (Clockwise)

Preceding gene: 119898690

Following gene: 119898692

Centisome position: 60.35

GC content: 66.13

Gene sequence:

>2217_bases
ATGGCCGGCTGGCTTGCGCTCGCCTGCCTTGCGCCGCTGAATGCGCGCGCTGCGGTAGCCGAATCCGATTTCGACAAGCT
GATGGCCTTGTCGCTGACGGAGTTGATCGAGACGCCGATCATCACCGCTTCGCGCCAGTCGGAAACCCGGGACCAGACGC
CCTCGCACATCATCGTGGTCACGCGCGAGCAGATCCGCGAACGCCGCTACAAGAACCTCGCGGACTTGCTCGAAGACATG
CCGGGGGTGGATTTCCAGCGCGGCACCAAGTCGTCGCAATACAACCAGTTCGCGGTGCAGGGCTCGCTCGGCCCGCACCG
GCTGGTGGTGATGCTGGACGGCGTGCGCATCGGCCATCCGTCCGGCGGCAACATGCCGATCGCCGAAAACCTGGCGCTGT
ACCACGCCAAGCAGGTGGAGTTCCTGTATGGGCCGGCGGCCGCGCTGTACGGGGCCGACGCGGTGTCGGGGGTCATCAAC
ATCATCACCGACCGCGGTAGCCGGGGCGCGGGCTCGTGGGTGGAGGTGGGCGCGGGCGACTTCGATACCCAGGAAACCTC
CTTCATGGCCGGCTACAGCGGCGAGCGCATGGCGTTCACCGTCGGCGGTCACCGCCAGCGCTCGGACCGCGCGCCGCTCG
ACCGTTACTACCCGAACGACTTCCGCAAGGTGGACGCCGATCCGGACTCGCAGCGGGTCGTGCCGGCGGCCGAGCGTGAA
GGCTATGTCGGCGACATTTCGAGCAACAGCCTCTTCGCCCGCCTCGATCTCGGCGACGACCTCACCTTCGGCTTCTATCG
CAACGTCTTCCGCAGCCTCACGAGCACCGGCGACCCCCCCGCCACCGCGCTCTACCTGCGCGACTCGCAATGGACCACCA
CCAGCGAGACCACCTACGGCAAGTACCGCTACACGCTCGGCGCCGACTTCTCGGGCGAGGTGGTGGTGGATTACTCGCGC
ATGCAGGTCGATCCGCATTCCAAGTACAACAACAGCTTCAGCAACTTTTCCGACGGCTATTCCTACGTGCTCGGCGAGCG
GATCGGCATCGAGCACAACATCAACTGGCGCTTCGACGACACCCACCGCGTGCAGGCCGGCCTGGGCTACCAGAAGCACT
ACGCGATCGAAACCGCGTCGCTGCCCGCGCCGTATGACACCGACCGCGGCAAGTCCGAGCAGGGCTACGTCTATCCGAAC
ACGACGATTCCGATGCAGATCTACGACGCCGCGTTCGAGAACGTGTCGATGTACGCGCAGCTGCAGTCGGAATGGGATGA
GGCGTTCTCGACCATGGTGGGCCTGCGCATGGACCGCCATTCCGACTACGGGCAGACGGTGAACCCGCGCCTCGGGGCCG
TATGGCGGCTCAATCCGCAGCATGTACTGAAGTTCCTGTACGGCGAGGCCTTCCGCGCGCCGTCGCCGGAAGAATCGCTG
AGCGCCTTCGGCATTTTCGACGGCTCGATGTCGAACGGGCTGTACGTCGGCCGCGGTTTCCGCGTGCCCAACTTCAACCT
CGACCCGGAAGAAGCGCGCACCACCAGCCTGACCTGGGACTGGCGGCCGCAGGCCAACCTCAACTTCGTCGCCAACCTCT
ACCACAGCCGGATCGAGAACCTGATCGTCACCCGGCCCTCCACCCAGGTGGACGCGATCCCCGGCGCGGTGCTGATCAAC
CCCGAAACCAAGGGCAACGCCGGCGTGCAGCGCCACTACGGTGTAGATCTGATGGCGCAGTGGCGTTTCAAGATCGACGA
GGCCTGGTCGGGCGACCTGTGGGGCAGCGCAAGCTGGATCCGCGGTCGCATCGACGAAGGCGACGGCGTGGAATGGGAGA
TTTCGCACGTGGCCGAGCGCAAGCTCAAGCTCGGCACCACCTTGCGCTACCGCGACCGCCTGAGCCTCACCACCCAGGTG
CTGGCGATGGGCGACACCACCAACGGCCGCCGCCGGGCCCCTGCGCCCTCGCTGCAGCTGGCGCAGACCTGTGACGCCGA
CGGCGCACCGGAACGCTGCCGGACCGACGGCTACACCGTGGTCAATTTCCACGCCGGCTGGCACAAGCTGTTCGACGGCC
GCGCGACCCTGTGGCTGGACATCTACAACCTGTTCGACACCCGCTACTACGCCGCCCACGGATCGGGCAGCCGCACCTTC
TGGGACATGCCGCAGCAGCCCCGCACCTCGATGATCTCGCTTGAATACCGGTTCTGA

Upstream 100 bases:

>100_bases
CGAATCCGAATAACAACCGAACGGCGGCCGCATGGCGCCACAGCGGCGATACCAGCGTATGAACAGACCCGTTTCCGGGC
CCTTGCCGAACACGCGCCTT

Downstream 100 bases:

>100_bases
GCGGCAGGCATGCACGTTTCCGTATTGACGAGGCCGATCCTGCTGCGCGTGCTGTTCGCGCTCGCCTGCCTGGCCGCGGT
GCTGGCACCGCCCTGCCTGG

Product: putative TonB-dependent receptor protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MAGWLALACLAPLNARAAVAESDFDKLMALSLTELIETPIITASRQSETRDQTPSHIIVVTREQIRERRYKNLADLLEDM
PGVDFQRGTKSSQYNQFAVQGSLGPHRLVVMLDGVRIGHPSGGNMPIAENLALYHAKQVEFLYGPAAALYGADAVSGVIN
IITDRGSRGAGSWVEVGAGDFDTQETSFMAGYSGERMAFTVGGHRQRSDRAPLDRYYPNDFRKVDADPDSQRVVPAAERE
GYVGDISSNSLFARLDLGDDLTFGFYRNVFRSLTSTGDPPATALYLRDSQWTTTSETTYGKYRYTLGADFSGEVVVDYSR
MQVDPHSKYNNSFSNFSDGYSYVLGERIGIEHNINWRFDDTHRVQAGLGYQKHYAIETASLPAPYDTDRGKSEQGYVYPN
TTIPMQIYDAAFENVSMYAQLQSEWDEAFSTMVGLRMDRHSDYGQTVNPRLGAVWRLNPQHVLKFLYGEAFRAPSPEESL
SAFGIFDGSMSNGLYVGRGFRVPNFNLDPEEARTTSLTWDWRPQANLNFVANLYHSRIENLIVTRPSTQVDAIPGAVLIN
PETKGNAGVQRHYGVDLMAQWRFKIDEAWSGDLWGSASWIRGRIDEGDGVEWEISHVAERKLKLGTTLRYRDRLSLTTQV
LAMGDTTNGRRRAPAPSLQLAQTCDADGAPERCRTDGYTVVNFHAGWHKLFDGRATLWLDIYNLFDTRYYAAHGSGSRTF
WDMPQQPRTSMISLEYRF

Sequences:

>Translated_738_residues
MAGWLALACLAPLNARAAVAESDFDKLMALSLTELIETPIITASRQSETRDQTPSHIIVVTREQIRERRYKNLADLLEDM
PGVDFQRGTKSSQYNQFAVQGSLGPHRLVVMLDGVRIGHPSGGNMPIAENLALYHAKQVEFLYGPAAALYGADAVSGVIN
IITDRGSRGAGSWVEVGAGDFDTQETSFMAGYSGERMAFTVGGHRQRSDRAPLDRYYPNDFRKVDADPDSQRVVPAAERE
GYVGDISSNSLFARLDLGDDLTFGFYRNVFRSLTSTGDPPATALYLRDSQWTTTSETTYGKYRYTLGADFSGEVVVDYSR
MQVDPHSKYNNSFSNFSDGYSYVLGERIGIEHNINWRFDDTHRVQAGLGYQKHYAIETASLPAPYDTDRGKSEQGYVYPN
TTIPMQIYDAAFENVSMYAQLQSEWDEAFSTMVGLRMDRHSDYGQTVNPRLGAVWRLNPQHVLKFLYGEAFRAPSPEESL
SAFGIFDGSMSNGLYVGRGFRVPNFNLDPEEARTTSLTWDWRPQANLNFVANLYHSRIENLIVTRPSTQVDAIPGAVLIN
PETKGNAGVQRHYGVDLMAQWRFKIDEAWSGDLWGSASWIRGRIDEGDGVEWEISHVAERKLKLGTTLRYRDRLSLTTQV
LAMGDTTNGRRRAPAPSLQLAQTCDADGAPERCRTDGYTVVNFHAGWHKLFDGRATLWLDIYNLFDTRYYAAHGSGSRTF
WDMPQQPRTSMISLEYRF
>Mature_737_residues
AGWLALACLAPLNARAAVAESDFDKLMALSLTELIETPIITASRQSETRDQTPSHIIVVTREQIRERRYKNLADLLEDMP
GVDFQRGTKSSQYNQFAVQGSLGPHRLVVMLDGVRIGHPSGGNMPIAENLALYHAKQVEFLYGPAAALYGADAVSGVINI
ITDRGSRGAGSWVEVGAGDFDTQETSFMAGYSGERMAFTVGGHRQRSDRAPLDRYYPNDFRKVDADPDSQRVVPAAEREG
YVGDISSNSLFARLDLGDDLTFGFYRNVFRSLTSTGDPPATALYLRDSQWTTTSETTYGKYRYTLGADFSGEVVVDYSRM
QVDPHSKYNNSFSNFSDGYSYVLGERIGIEHNINWRFDDTHRVQAGLGYQKHYAIETASLPAPYDTDRGKSEQGYVYPNT
TIPMQIYDAAFENVSMYAQLQSEWDEAFSTMVGLRMDRHSDYGQTVNPRLGAVWRLNPQHVLKFLYGEAFRAPSPEESLS
AFGIFDGSMSNGLYVGRGFRVPNFNLDPEEARTTSLTWDWRPQANLNFVANLYHSRIENLIVTRPSTQVDAIPGAVLINP
ETKGNAGVQRHYGVDLMAQWRFKIDEAWSGDLWGSASWIRGRIDEGDGVEWEISHVAERKLKLGTTLRYRDRLSLTTQVL
AMGDTTNGRRRAPAPSLQLAQTCDADGAPERCRTDGYTVVNFHAGWHKLFDGRATLWLDIYNLFDTRYYAAHGSGSRTFW
DMPQQPRTSMISLEYRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=143, Percent_Identity=31.4685314685315, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788478, Length=151, Percent_Identity=27.8145695364238, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 82807; Mature: 82676

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGWLALACLAPLNARAAVAESDFDKLMALSLTELIETPIITASRQSETRDQTPSHIIVV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEE
TREQIRERRYKNLADLLEDMPGVDFQRGTKSSQYNQFAVQGSLGPHRLVVMLDGVRIGHP
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCEEECCC
SGGNMPIAENLALYHAKQVEFLYGPAAALYGADAVSGVINIITDRGSRGAGSWVEVGAGD
CCCCCCHHHCHHHEEHEEEEEEECCHHHHHCHHHHHHHHHHEECCCCCCCCCEEEECCCC
FDTQETSFMAGYSGERMAFTVGGHRQRSDRAPLDRYYPNDFRKVDADPDSQRVVPAAERE
CCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCEECCCCCCCCCCCCCHHCC
GYVGDISSNSLFARLDLGDDLTFGFYRNVFRSLTSTGDPPATALYLRDSQWTTTSETTYG
CCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
KYRYTLGADFSGEVVVDYSRMQVDPHSKYNNSFSNFSDGYSYVLGERIGIEHNINWRFDD
EEEEEECCCCCCCEEEEEEHEEECCCHHCCCCCCCCCCCHHEEHHHHCCCEECCCEEECC
THRVQAGLGYQKHYAIETASLPAPYDTDRGKSEQGYVYPNTTIPMQIYDAAFENVSMYAQ
CHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHH
LQSEWDEAFSTMVGLRMDRHSDYGQTVNPRLGAVWRLNPQHVLKFLYGEAFRAPSPEESL
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHC
SAFGIFDGSMSNGLYVGRGFRVPNFNLDPEEARTTSLTWDWRPQANLNFVANLYHSRIEN
CEEEEECCCCCCCEEEECCEECCCCCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHH
LIVTRPSTQVDAIPGAVLINPETKGNAGVQRHYGVDLMAQWRFKIDEAWSGDLWGSASWI
EEEECCCCCCCCCCCEEEECCCCCCCCCCEECCCCCEEEEEEEEECCCCCCCCCCCCHHH
RGRIDEGDGVEWEISHVAERKLKLGTTLRYRDRLSLTTQVLAMGDTTNGRRRAPAPSLQL
EEECCCCCCCEEEHHHHHHHHHHHCCEEEEHHHHCEEEEEEEECCCCCCCCCCCCCCCHH
AQTCDADGAPERCRTDGYTVVNFHAGWHKLFDGRATLWLDIYNLFDTRYYAAHGSGSRTF
HHHCCCCCCCHHHCCCCEEEEEECCCHHHHHCCCEEEEEEEEHHHCCEEEEECCCCCCEE
WDMPQQPRTSMISLEYRF
CCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
AGWLALACLAPLNARAAVAESDFDKLMALSLTELIETPIITASRQSETRDQTPSHIIVV
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEE
TREQIRERRYKNLADLLEDMPGVDFQRGTKSSQYNQFAVQGSLGPHRLVVMLDGVRIGHP
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCEEECCC
SGGNMPIAENLALYHAKQVEFLYGPAAALYGADAVSGVINIITDRGSRGAGSWVEVGAGD
CCCCCCHHHCHHHEEHEEEEEEECCHHHHHCHHHHHHHHHHEECCCCCCCCCEEEECCCC
FDTQETSFMAGYSGERMAFTVGGHRQRSDRAPLDRYYPNDFRKVDADPDSQRVVPAAERE
CCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCEECCCCCCCCCCCCCHHCC
GYVGDISSNSLFARLDLGDDLTFGFYRNVFRSLTSTGDPPATALYLRDSQWTTTSETTYG
CCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
KYRYTLGADFSGEVVVDYSRMQVDPHSKYNNSFSNFSDGYSYVLGERIGIEHNINWRFDD
EEEEEECCCCCCCEEEEEEHEEECCCHHCCCCCCCCCCCHHEEHHHHCCCEECCCEEECC
THRVQAGLGYQKHYAIETASLPAPYDTDRGKSEQGYVYPNTTIPMQIYDAAFENVSMYAQ
CHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHH
LQSEWDEAFSTMVGLRMDRHSDYGQTVNPRLGAVWRLNPQHVLKFLYGEAFRAPSPEESL
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHC
SAFGIFDGSMSNGLYVGRGFRVPNFNLDPEEARTTSLTWDWRPQANLNFVANLYHSRIEN
CEEEEECCCCCCCEEEECCEECCCCCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHH
LIVTRPSTQVDAIPGAVLINPETKGNAGVQRHYGVDLMAQWRFKIDEAWSGDLWGSASWI
EEEECCCCCCCCCCCEEEECCCCCCCCCCEECCCCCEEEEEEEEECCCCCCCCCCCCHHH
RGRIDEGDGVEWEISHVAERKLKLGTTLRYRDRLSLTTQVLAMGDTTNGRRRAPAPSLQL
EEECCCCCCCEEEHHHHHHHHHHHCCEEEEHHHHCEEEEEEEECCCCCCCCCCCCCCCHH
AQTCDADGAPERCRTDGYTVVNFHAGWHKLFDGRATLWLDIYNLFDTRYYAAHGSGSRTF
HHHCCCCCCCHHHCCCCEEEEEECCCHHHHHCCCEEEEEEEEHHHCCEEEEECCCCCCEE
WDMPQQPRTSMISLEYRF
CCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA