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The map label for this gene is argG [H]

Identifier: 119898477

GI number: 119898477

Start: 2392276

End: 2393505

Strand: Reverse

Name: argG [H]

Synonym: azo2186

Alternate gene names: 119898477

Gene position: 2393505-2392276 (Counterclockwise)

Preceding gene: 119898478

Following gene: 119898476

Centisome position: 54.7

GC content: 62.6

Gene sequence:

>1230_bases
ATGAGCGACGTGAAGAAAGTAGTGTTGGCCTACTCGGGCGGCCTCGATACCTCGGTGATCCTGAAGTGGCTGCAGGATAC
CTATCAGTGTGAAGTGGTGACCTTTACGGCCGACCTCGGCCAGGGCGAGGAACTCGAGCCGGCGCGCCAGAAGGCACTCA
AGTTCGGCATCAAGCCGGAGAACATCTTCATCGACGACCTGCGCGAGGAATTCGTCCGCGATTTCGTGTTCCCGATGTTC
CGCGCCAACACGATCTACGAGGGCGAGTACCTCCTTGGCACCTCGATCGCCCGTCCGCTGATCGCCAAGCGTCAGATCGA
GATCGCGCGCGCCACCGGCGCCGACGCCGTGTCGCACGGTGCCACCGGCAAGGGCAATGACCAGGTCCGCTTCGAACTCG
GCTATTACGCGCTGATGCCCGGCGTCAAGGTGATCGCGCCGTGGCGCGAGTGGGACCTGCTGTCGCGCGAGAAACTGCTG
GCGTACGCCGAGAAGCACGGCATTCCCATCGAGATGAAGCACAAGCAGGGCGGTTCGCCGTATTCGATGGACGCCAACCT
GCTGCACATCTCCTTCGAAGGCCGCCATCTCGAAAACCCGGCTGCGGAAGCCGAAGAGTCGATGTGGCGCTGGACCGTGT
CGCCGGAAGCAGCGCCCGATGCGGCGGAGTACGTCGATCTCGAGTTCGAGAAGGGCGATCTGGTCGCCATCAACGGTCAG
CGCCTGAAGGCGCATGAACTCCTCGCCAAGCTCAACGAGCTGGGTGGCAAGCACGGTATCGGTCGCCTGGATCTGGTGGA
AAACCGCTACGTCGGCATGAAGTCGCGCGGTTGCTACGAAACGCCGGGAGGCACGATCCTGCTGCGCGCCCACCGCGCCA
TCGAATCGATCACGCTGGACCGCGAGGTTGCCCACCTCAAGGACGACCTCATGCCGCGCTACGCCAGCATGATCTACAAC
GGCTACTGGTGGAGCCCGGAACGCCAGGCACTGCAGGCGCTGGTCGATCACACGCAGCAAACGGTTAACGGCTGGGTGCG
CATCAAGCTCTACAAGGGCAACGTCATCGTCGTTGCGCGCGACTCCAAGACCGATTCGCTGTTCGATCCGACGATTGCCA
CCTTCGAGGACGATCAGGGCGCCTACAACCAGAAGGACGCGCACGGTTTCATCCGCCTCAACGCGCTGCGCATGCGCATT
GCTGCCAACGCGAAGACCAAGCGGGGTTAA

Upstream 100 bases:

>100_bases
TCGTTCTCGGTCGCGTCGGGGACGAAGAGTCGAACTAACGCGAATTGCTGCTAACCGCGGGCGAGCTGCAGTTTCGCCCG
CAATCAGACTGGACATGACG

Downstream 100 bases:

>100_bases
GGAGCCGCCGACATGGAAGACCCGCTGATCTTCGGGCTGACGATTGCCGAGTTCGAGGATATCTCGCTCAAGGTCTGCTT
CACGGCGCTGATCCTGTACA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALKFGIKPENIFIDDLREEFVRDFVFPMF
RANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLL
AYAEKHGIPIEMKHKQGGSPYSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYVDLEFEKGDLVAINGQ
RLKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYN
GYWWSPERQALQALVDHTQQTVNGWVRIKLYKGNVIVVARDSKTDSLFDPTIATFEDDQGAYNQKDAHGFIRLNALRMRI
AANAKTKRG

Sequences:

>Translated_409_residues
MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALKFGIKPENIFIDDLREEFVRDFVFPMF
RANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLL
AYAEKHGIPIEMKHKQGGSPYSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYVDLEFEKGDLVAINGQ
RLKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYN
GYWWSPERQALQALVDHTQQTVNGWVRIKLYKGNVIVVARDSKTDSLFDPTIATFEDDQGAYNQKDAHGFIRLNALRMRI
AANAKTKRG
>Mature_408_residues
SDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALKFGIKPENIFIDDLREEFVRDFVFPMFR
ANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLLA
YAEKHGIPIEMKHKQGGSPYSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYVDLEFEKGDLVAINGQR
LKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYNG
YWWSPERQALQALVDHTQQTVNGWVRIKLYKGNVIVVARDSKTDSLFDPTIATFEDDQGAYNQKDAHGFIRLNALRMRIA
ANAKTKRG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=46.8137254901961, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=46.8137254901961, Blast_Score=356, Evalue=2e-98,
Organism=Escherichia coli, GI1789563, Length=418, Percent_Identity=26.555023923445, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=418, Percent_Identity=43.3014354066986, Blast_Score=350, Evalue=3e-97,
Organism=Drosophila melanogaster, GI21358151, Length=405, Percent_Identity=45.4320987654321, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 46056; Mature: 45925

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALKFGIKPE
CCCHHEEEEEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
NIFIDDLREEFVRDFVFPMFRANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHG
CEEHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHEEEEECCCCCHHHCC
ATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLLAYAEKHGIPIEMKHKQGGSP
CCCCCCCEEEEEECEEEECCCCEEEECCHHHCHHHHHHHHHHHHHCCCCEEEECCCCCCC
YSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYVDLEFEKGDLVAINGQ
CEECCEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCCCCCHHEEEEEECCCCEEEECCC
RLKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLD
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCH
REVAHLKDDLMPRYASMIYNGYWWSPERQALQALVDHTQQTVNGWVRIKLYKGNVIVVAR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEE
DSKTDSLFDPTIATFEDDQGAYNQKDAHGFIRLNALRMRIAANAKTKRG
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALKFGIKPE
CCHHEEEEEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
NIFIDDLREEFVRDFVFPMFRANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHG
CEEHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHEEEEECCCCCHHHCC
ATGKGNDQVRFELGYYALMPGVKVIAPWREWDLLSREKLLAYAEKHGIPIEMKHKQGGSP
CCCCCCCEEEEEECEEEECCCCEEEECCHHHCHHHHHHHHHHHHHCCCCEEEECCCCCCC
YSMDANLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYVDLEFEKGDLVAINGQ
CEECCEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCCCCCHHEEEEEECCCCEEEECCC
RLKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLD
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCH
REVAHLKDDLMPRYASMIYNGYWWSPERQALQALVDHTQQTVNGWVRIKLYKGNVIVVAR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEE
DSKTDSLFDPTIATFEDDQGAYNQKDAHGFIRLNALRMRIAANAKTKRG
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA