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The map label for this gene is argF

Identifier: 119898478

GI number: 119898478

Start: 2393568

End: 2394503

Strand: Reverse

Name: argF

Synonym: azo2187

Alternate gene names: 119898478

Gene position: 2394503-2393568 (Counterclockwise)

Preceding gene: 119898479

Following gene: 119898477

Centisome position: 54.72

GC content: 62.07

Gene sequence:

>936_bases
ATGAGTTCGCCCAGACACTATCTTCAGTTCAGCGATTTCACCGCTGCGGAGTACGACCACGTGTTCCAGCGGACGCGCTG
GATCAAGGAAAAGTTCAAGCAGTATGAGCCGTATCACCCCCTGTTCGACCGCACGCTCGTCATGATCTTCGAGAAGGCGA
GTACTCGTACCCGCCTCTCGTTCGAAGCCGGCATGCAGCAACTGGGCGGCTCCGCAATCTATCTGAATACGCGCGACTCC
CAACTGGGGCGCGGCGAGCCGGTGGAGGACGCCGCCCAGGTGATCTCCCGCATGAGCGACCTGGTGATGATCCGCACTTT
CGAGCAGGACATCATCGAACGGTTCGCGGCCTATTCCCGGGTGCCGGTGATCAACGGCCTCACCAACGAATACCATCCCT
GCCAGATCCTCGCGGATATCTACACCTTCATCGAGCACCGCGGCAGCATCAAGGGCAAGACGGTGGCCTGGGTGGGGGAT
TCGAACAACATGTGCAACACCTGGCTGCAGGCGGCCGAGGTGCTGGATTTCAAGGTGCGGGTGTCGACGCCGCCGGGATA
CGAGGTCGAACCGACCGACGCAGGTCTGCGTGGCACCGCTCACTTCGCCCAATTCTCCGATCCGCTCGAAGCCTGCCGCG
GCGCCGATCTCGTCACCACCGACGTCTGGACCTCGATGGGTTTCGAGGCGGAGAACGAGGCGCGGATGAAGGCTTTCGCC
GACTGGTGCGTCGACGCTGAAATGATGTCGGTCGCCGCGCCGGACGCGCTGTTCATGCACTGCCTGCCGGCTCACCGCGG
CGAGGAAGTCACCGCGGAAGTGATCGACGGTCCGCAGTCGGTGGTATGGGATGAGGCCGAAAACCGCCTGCACGTGCAGA
AGGCCCTGATGGAATATCTCGTTCTCGGTCGCGTCGGGGACGAAGAGTCGAACTAA

Upstream 100 bases:

>100_bases
CCGCGCTCGTCTTCACCGCGGCCGACGCCCAAGCCCTCGTCGGCGCGCTGGCGCCGCTCATCCGCGACTTCCTGTCGCAG
CAAGGAAAAGGTACTGCGTG

Downstream 100 bases:

>100_bases
CGCGAATTGCTGCTAACCGCGGGCGAGCTGCAGTTTCGCCCGCAATCAGACTGGACATGACGATGAGCGACGTGAAGAAA
GTAGTGTTGGCCTACTCGGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSSPRHYLQFSDFTAAEYDHVFQRTRWIKEKFKQYEPYHPLFDRTLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDS
QLGRGEPVEDAAQVISRMSDLVMIRTFEQDIIERFAAYSRVPVINGLTNEYHPCQILADIYTFIEHRGSIKGKTVAWVGD
SNNMCNTWLQAAEVLDFKVRVSTPPGYEVEPTDAGLRGTAHFAQFSDPLEACRGADLVTTDVWTSMGFEAENEARMKAFA
DWCVDAEMMSVAAPDALFMHCLPAHRGEEVTAEVIDGPQSVVWDEAENRLHVQKALMEYLVLGRVGDEESN

Sequences:

>Translated_311_residues
MSSPRHYLQFSDFTAAEYDHVFQRTRWIKEKFKQYEPYHPLFDRTLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDS
QLGRGEPVEDAAQVISRMSDLVMIRTFEQDIIERFAAYSRVPVINGLTNEYHPCQILADIYTFIEHRGSIKGKTVAWVGD
SNNMCNTWLQAAEVLDFKVRVSTPPGYEVEPTDAGLRGTAHFAQFSDPLEACRGADLVTTDVWTSMGFEAENEARMKAFA
DWCVDAEMMSVAAPDALFMHCLPAHRGEEVTAEVIDGPQSVVWDEAENRLHVQKALMEYLVLGRVGDEESN
>Mature_310_residues
SSPRHYLQFSDFTAAEYDHVFQRTRWIKEKFKQYEPYHPLFDRTLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDSQ
LGRGEPVEDAAQVISRMSDLVMIRTFEQDIIERFAAYSRVPVINGLTNEYHPCQILADIYTFIEHRGSIKGKTVAWVGDS
NNMCNTWLQAAEVLDFKVRVSTPPGYEVEPTDAGLRGTAHFAQFSDPLEACRGADLVTTDVWTSMGFEAENEARMKAFAD
WCVDAEMMSVAAPDALFMHCLPAHRGEEVTAEVIDGPQSVVWDEAENRLHVQKALMEYLVLGRVGDEESN

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=40, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=28.1045751633987, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=36, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1786469, Length=325, Percent_Identity=35.6923076923077, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=26.3322884012539, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI48994908, Length=315, Percent_Identity=24.7619047619048, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=325, Percent_Identity=27.6923076923077, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=321, Percent_Identity=34.5794392523364, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=27.0700636942675, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=26.6881028938907, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_AZOSB (A1K7J9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933691.1
- ProteinModelPortal:   A1K7J9
- SMR:   A1K7J9
- STRING:   A1K7J9
- GeneID:   4608336
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2187
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   AKFLHCL
- PhylomeDB:   A1K7J9
- ProtClustDB:   PRK00779
- BioCyc:   ASP62928:AZO2187-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 35240; Mature: 35109

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRHYLQFSDFTAAEYDHVFQRTRWIKEKFKQYEPYHPLFDRTLVMIFEKASTRTRLS
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
FEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDLVMIRTFEQDIIERFAAYSR
HHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVINGLTNEYHPCQILADIYTFIEHRGSIKGKTVAWVGDSNNMCNTWLQAAEVLDFKVR
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEE
VSTPPGYEVEPTDAGLRGTAHFAQFSDPLEACRGADLVTTDVWTSMGFEAENEARMKAFA
ECCCCCCEECCCCCCCCCCHHHHHHCCHHHHHCCCCEEEHHHHHHCCCCCCCHHHHHHHH
DWCVDAEMMSVAAPDALFMHCLPAHRGEEVTAEVIDGPQSVVWDEAENRLHVQKALMEYL
HHHHCHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VLGRVGDEESN
HHHCCCCCCCC
>Mature Secondary Structure 
SSPRHYLQFSDFTAAEYDHVFQRTRWIKEKFKQYEPYHPLFDRTLVMIFEKASTRTRLS
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
FEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDLVMIRTFEQDIIERFAAYSR
HHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVINGLTNEYHPCQILADIYTFIEHRGSIKGKTVAWVGDSNNMCNTWLQAAEVLDFKVR
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEE
VSTPPGYEVEPTDAGLRGTAHFAQFSDPLEACRGADLVTTDVWTSMGFEAENEARMKAFA
ECCCCCCEECCCCCCCCCCHHHHHHCCHHHHHCCCCEEEHHHHHHCCCCCCCHHHHHHHH
DWCVDAEMMSVAAPDALFMHCLPAHRGEEVTAEVIDGPQSVVWDEAENRLHVQKALMEYL
HHHHCHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VLGRVGDEESN
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA