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The map label for this gene is ppsA1 [H]

Identifier: 119898458

GI number: 119898458

Start: 2373743

End: 2376109

Strand: Reverse

Name: ppsA1 [H]

Synonym: azo2167

Alternate gene names: 119898458

Gene position: 2376109-2373743 (Counterclockwise)

Preceding gene: 119898463

Following gene: 119898457

Centisome position: 54.3

GC content: 63.96

Gene sequence:

>2367_bases
ATGACCCGCTATGTCATCCCCTTCACCGAGCTGCGCATGACCGATGTCGATCAGGTCGGCGGCAAGAATGCTTCGCTCGG
CGAAATGATCAGCCAACTGCCGTCCAGCGTCCGCGTTCCCGGTGGTTTCGCCACCACCGCGGCTGCATATCGCGAGTTCC
TCGCGCACGGCGGATTGGCCGACCGGATCAACGCCGCGCTCGATACCCTCGACGTGGATGACGTGGATGCGCTGGTGAAG
ACCGGCGCGCAGATCCGCCAGTGGATCGTCGATACCCCGTTCCCCGCGGCGCTCGAAGCCGACATCAAGGCCGCGTACGA
AAAGATCACCGCCGAAGGCGAGGGCAGCTTCGCGGTGCGTTCGTCCGCTACCGCCGAAGACCTTCCGGACGCCTCCTTCG
CCGGTCAGCAGGAAACCTTCCTGAACATCCACGGCTACGAGAACATCCTGCACGCGATGAAGGAAGTGTTCGCGTCGCTG
TACAACGACCGTGCCATCGCCTACCGGGTGCACAAGAACTTCGCCCACGCCGAGGTTGCCCTGTCGGCCGGCGTGCAGCG
CATGGTGCGCTCCGATACCGGTGCCTCGGGCGTCATGTTCACCATCGACACCGAATCCGGCTTCTCCGACGTGGTGTTCA
TCACCGCGTCCTACGGTCTGGGCGAAACCGTCGTGCAAGGCGCGGTGAACCCGGACGAGTTCTATGTCCACAAGCCCACG
CTGGCCCTGAACAAGCCCGCGGTCATCCGCCGCAACCTGGGCTCCAAGCTGATCAAGATGGTGTTCGCGGACAAGGCCGT
GGCCGGCAAGTCGGTGCGTACGGTGGACGTGCCCGAAGCCGACCGCAACCGCTTCTCGCTGACCGACGAAGACGTGCTCG
AACTTGCCCGCTATGCGGTCATCATCGAGAAGCACTACGGCCGCCCGATGGACATCGAGTGGGGCAAGGACGGCGAGGAC
GGCAAGCTGTACATCCTGCAGGCGCGCCCGGAAACCGTTAAGAGCCAGAGCAACGGCCTGGTGATGGAGAAGTACCGCCT
CAAGCAGTACGGCAAGGCCCTTACCCACGGTCGTGCCATCGGCCAGAAGATCGGCGCCGGCACCGTGCGCGTGGTCAAGG
ACGCCTCCGAGATGAACCGCGTCCAGGCCGGCGACATCCTCGTCACCGACATGACCGACCCGAACTGGGAGCCGGTGATG
AAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGCCGTACCTGCCACGCGGCGATCATCGCGCGCGAACTCGGCATTCC
GGCAATCGTCGGCTGCGGCAACGCCACCGAGGTGCTGGAAGAGGGCGATTCCGTCACCGTGTCCTGCGCCGAAGGCGACA
CCGGCTACGTCTATCGCGGCAAGCTGGAGTTCGAAGTGATCACCACCGACATGGGCAACCTGCCTGAAATCCCGGTGAAG
ATCATGATGAACGTCGGCAATCCGGAACTCGCCTTCGAGTTCGCCCAGATTCCGAACGGCGGCGTCGGCCTCGCGCGGCT
GGAGTTCGTCATCAACAACATGATCGGCATTCACCCGAAGGCCATCCTCGAGCTCTCGCAAGTGCCGGCCAGCCTGCGTG
ACGAAATCACCCGCCGCTCGCGCGGCTACGCCACGCCCAAGCAGTTCTTCATCGAGAAGCTGGCCGAAGGCGTCGCCACC
ATCGCCGCCGCCTTCTATCCGAAGCCGGTCATCGTCCGCATGTCGGACTTCAAGTCGAACGAGTACCGCAAGCTGCTCGG
CGGCGAAATCTACGAGCCGGAAGAAGAAAACCCGATGCTCGGTTTCCGCGGCGCGTCGCGCTACATCGCCCACAGCTTCC
GCGACTGTTTCGAGCTGGAATGCGCGGCGATGAAGAAGGTGCGCAACGAACTCGGTCTGACCAACGTGCAGATCATGATT
CCGTTCGTGCGCAACGTCGAAGAAGCGGCCGGTGTGGTGGACCTGCTCGCCGAGCACGGCCTGAAGCGCGGCGTGAACGA
TCTCAAGCTGATCATGATGTGCGAGATCCCGTCCAACGCGCTGCTGGCGGACAAGTTCCTCGAGCACTTCGACGGCTTCT
CGATCGGGTCCAACGACCTCACCCAGCTCACGCTCGGCCTCGACCGCGACTCGGGTCTCGTCGCCCACGCGTTCGACGAG
CGCGACCCCGCGGTCAAGCTGCTGCTGTCGATGGCCATCTCCACCGCGAACCGGCTGGGCAAGTATGTCGGTATCTGCGG
CCAGGGCCCGTCGGACCACGCCGATTTCGCGGAGTGGCTGATGGACGAAGGCATCCAGACCATTTCGCTCAACCCGGATA
CCGTGGTCGATACCTGGCTGAAGCTGGCCTCGCACGGCAAGCAGTAA

Upstream 100 bases:

>100_bases
CTCAAGGCGGGTATTGGGGCAAACCCTGCCGTGCGGTGCAGCACCGATTCTCGTTAAAATCGCATTTTGCTTTTTCTTTG
TTCATTCCGGAAGGCCATCC

Downstream 100 bases:

>100_bases
GCAGTCGCAACGTACAGGAAGCGGCGGCCTGCGGGCCGCCGTTTTTTTTGCCGGGGCGGGCGTACAAATCCGCACTTGCT
CCGGGCTACCACTATGGTGT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MTRYVIPFTELRMTDVDQVGGKNASLGEMISQLPSSVRVPGGFATTAAAYREFLAHGGLADRINAALDTLDVDDVDALVK
TGAQIRQWIVDTPFPAALEADIKAAYEKITAEGEGSFAVRSSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASL
YNDRAIAYRVHKNFAHAEVALSAGVQRMVRSDTGASGVMFTIDTESGFSDVVFITASYGLGETVVQGAVNPDEFYVHKPT
LALNKPAVIRRNLGSKLIKMVFADKAVAGKSVRTVDVPEADRNRFSLTDEDVLELARYAVIIEKHYGRPMDIEWGKDGED
GKLYILQARPETVKSQSNGLVMEKYRLKQYGKALTHGRAIGQKIGAGTVRVVKDASEMNRVQAGDILVTDMTDPNWEPVM
KRASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATEVLEEGDSVTVSCAEGDTGYVYRGKLEFEVITTDMGNLPEIPVK
IMMNVGNPELAFEFAQIPNGGVGLARLEFVINNMIGIHPKAILELSQVPASLRDEITRRSRGYATPKQFFIEKLAEGVAT
IAAAFYPKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFELECAAMKKVRNELGLTNVQIMI
PFVRNVEEAAGVVDLLAEHGLKRGVNDLKLIMMCEIPSNALLADKFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAHAFDE
RDPAVKLLLSMAISTANRLGKYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWLKLASHGKQ

Sequences:

>Translated_788_residues
MTRYVIPFTELRMTDVDQVGGKNASLGEMISQLPSSVRVPGGFATTAAAYREFLAHGGLADRINAALDTLDVDDVDALVK
TGAQIRQWIVDTPFPAALEADIKAAYEKITAEGEGSFAVRSSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASL
YNDRAIAYRVHKNFAHAEVALSAGVQRMVRSDTGASGVMFTIDTESGFSDVVFITASYGLGETVVQGAVNPDEFYVHKPT
LALNKPAVIRRNLGSKLIKMVFADKAVAGKSVRTVDVPEADRNRFSLTDEDVLELARYAVIIEKHYGRPMDIEWGKDGED
GKLYILQARPETVKSQSNGLVMEKYRLKQYGKALTHGRAIGQKIGAGTVRVVKDASEMNRVQAGDILVTDMTDPNWEPVM
KRASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATEVLEEGDSVTVSCAEGDTGYVYRGKLEFEVITTDMGNLPEIPVK
IMMNVGNPELAFEFAQIPNGGVGLARLEFVINNMIGIHPKAILELSQVPASLRDEITRRSRGYATPKQFFIEKLAEGVAT
IAAAFYPKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFELECAAMKKVRNELGLTNVQIMI
PFVRNVEEAAGVVDLLAEHGLKRGVNDLKLIMMCEIPSNALLADKFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAHAFDE
RDPAVKLLLSMAISTANRLGKYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWLKLASHGKQ
>Mature_787_residues
TRYVIPFTELRMTDVDQVGGKNASLGEMISQLPSSVRVPGGFATTAAAYREFLAHGGLADRINAALDTLDVDDVDALVKT
GAQIRQWIVDTPFPAALEADIKAAYEKITAEGEGSFAVRSSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASLY
NDRAIAYRVHKNFAHAEVALSAGVQRMVRSDTGASGVMFTIDTESGFSDVVFITASYGLGETVVQGAVNPDEFYVHKPTL
ALNKPAVIRRNLGSKLIKMVFADKAVAGKSVRTVDVPEADRNRFSLTDEDVLELARYAVIIEKHYGRPMDIEWGKDGEDG
KLYILQARPETVKSQSNGLVMEKYRLKQYGKALTHGRAIGQKIGAGTVRVVKDASEMNRVQAGDILVTDMTDPNWEPVMK
RASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATEVLEEGDSVTVSCAEGDTGYVYRGKLEFEVITTDMGNLPEIPVKI
MMNVGNPELAFEFAQIPNGGVGLARLEFVINNMIGIHPKAILELSQVPASLRDEITRRSRGYATPKQFFIEKLAEGVATI
AAAFYPKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFELECAAMKKVRNELGLTNVQIMIP
FVRNVEEAAGVVDLLAEHGLKRGVNDLKLIMMCEIPSNALLADKFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAHAFDER
DPAVKLLLSMAISTANRLGKYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWLKLASHGKQ

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=785, Percent_Identity=66.6242038216561, Blast_Score=1103, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=383, Percent_Identity=26.1096605744125, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.4607329842932, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI48994992, Length=164, Percent_Identity=32.3170731707317, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789193, Length=401, Percent_Identity=24.9376558603491, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=252, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86067; Mature: 85936

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYVIPFTELRMTDVDQVGGKNASLGEMISQLPSSVRVPGGFATTAAAYREFLAHGGLA
CCEEEECHHHHCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHH
DRINAALDTLDVDDVDALVKTGAQIRQWIVDTPFPAALEADIKAAYEKITAEGEGSFAVR
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
SSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASLYNDRAIAYRVHKNFAHAEVA
CCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
LSAGVQRMVRSDTGASGVMFTIDTESGFSDVVFITASYGLGETVVQGAVNPDEFYVHKPT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEECCC
LALNKPAVIRRNLGSKLIKMVFADKAVAGKSVRTVDVPEADRNRFSLTDEDVLELARYAV
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IIEKHYGRPMDIEWGKDGEDGKLYILQARPETVKSQSNGLVMEKYRLKQYGKALTHGRAI
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHH
GQKIGAGTVRVVKDASEMNRVQAGDILVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAII
HHHCCCCCEEEECCHHHHCCEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
ARELGIPAIVGCGNATEVLEEGDSVTVSCAEGDTGYVYRGKLEFEVITTDMGNLPEIPVK
HHHCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCEE
IMMNVGNPELAFEFAQIPNGGVGLARLEFVINNMIGIHPKAILELSQVPASLRDEITRRS
EEEECCCHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
RGYATPKQFFIEKLAEGVATIAAAFYPKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPML
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCE
GFRGASRYIAHSFRDCFELECAAMKKVRNELGLTNVQIMIPFVRNVEEAAGVVDLLAEHG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHHHHH
LKRGVNDLKLIMMCEIPSNALLADKFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAHAFDE
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEECCC
RDPAVKLLLSMAISTANRLGKYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWL
CCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHH
KLASHGKQ
HHHHCCCC
>Mature Secondary Structure 
TRYVIPFTELRMTDVDQVGGKNASLGEMISQLPSSVRVPGGFATTAAAYREFLAHGGLA
CEEEECHHHHCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHH
DRINAALDTLDVDDVDALVKTGAQIRQWIVDTPFPAALEADIKAAYEKITAEGEGSFAVR
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
SSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASLYNDRAIAYRVHKNFAHAEVA
CCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
LSAGVQRMVRSDTGASGVMFTIDTESGFSDVVFITASYGLGETVVQGAVNPDEFYVHKPT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEECCC
LALNKPAVIRRNLGSKLIKMVFADKAVAGKSVRTVDVPEADRNRFSLTDEDVLELARYAV
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IIEKHYGRPMDIEWGKDGEDGKLYILQARPETVKSQSNGLVMEKYRLKQYGKALTHGRAI
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHH
GQKIGAGTVRVVKDASEMNRVQAGDILVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAII
HHHCCCCCEEEECCHHHHCCEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
ARELGIPAIVGCGNATEVLEEGDSVTVSCAEGDTGYVYRGKLEFEVITTDMGNLPEIPVK
HHHCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCEE
IMMNVGNPELAFEFAQIPNGGVGLARLEFVINNMIGIHPKAILELSQVPASLRDEITRRS
EEEECCCHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
RGYATPKQFFIEKLAEGVATIAAAFYPKPVIVRMSDFKSNEYRKLLGGEIYEPEEENPML
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCE
GFRGASRYIAHSFRDCFELECAAMKKVRNELGLTNVQIMIPFVRNVEEAAGVVDLLAEHG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHHHHH
LKRGVNDLKLIMMCEIPSNALLADKFLEHFDGFSIGSNDLTQLTLGLDRDSGLVAHAFDE
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEECCC
RDPAVKLLLSMAISTANRLGKYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWL
CCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHH
KLASHGKQ
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]