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The map label for this gene is thlA [H]

Identifier: 119898463

GI number: 119898463

Start: 2380606

End: 2381787

Strand: Reverse

Name: thlA [H]

Synonym: azo2172

Alternate gene names: 119898463

Gene position: 2381787-2380606 (Counterclockwise)

Preceding gene: 119898464

Following gene: 119898458

Centisome position: 54.43

GC content: 65.99

Gene sequence:

>1182_bases
ATGGCAAGTCGTGAAGTTGTGGTTTTGAGCGCGGTGCGCTCGGCTGTTGGCGGCTTTGGCGGTTCGCTCAAGGACATGGA
GCCGGCCGAACTCGGCGGCGTCGTGGTCAAGGAAGCGATCTCGCGCGCCGGCGTCGATCCGAAGCAGGTCACGTTCGCTA
CCGTGGGTAACTGCATCCCGACGGAGTCCCGCTATCCATACGTGGCGCGTGTCGCCACCATCCAGGGCGGCATGTCGATG
GAGTCGGTCGCTTTCGCGGTCAACCGGCTGTGTGGCTCGGCGCAGCAGGCCATCGTCAACTCGGCCCAGGCCATTCTGCT
CGGCGACGCCGATTACGCTGTGGGCGGCGGGGTCGAAGTCATGTCCCGCGGTGCGTACCTGATGCCGGCGCTGCGCAACG
GCGCCCGCATGGGCGACACCAAGGCCATCGACGCCATGGTGGCGGTGCTGACGGATCCGTTCGGTGTTGGCCACATGGGC
ATTACCGCTGAAAATCTGGCTGCCAAGTGGGGCATCAGCCGCGAAGAGCAGGACGCTTTCGCGCTCGAATCGCAGCGCCG
CGCCGCCGAGGCCATCGCCGACGGCCGCTTCAAGGGCCAGATCGTTCCGATCACCTTCGAAACCCGCAAGGGGCCCGTCG
TGTTCGATACCGACGAGCATCCGCGCGCGACCACGATGGAAGCGCTCGCGAAGATGAAGCCGGCGTTCAAGAAGGATGGC
AGCGTTACCGCAGGCAACGCCTCGGGCATCAACGATGCGGCCGCTTTCCTGGTGCTGGCCGACGCCGCGAAGGCTTCCGC
CGCTGGCCACAAGCCGATGGCGCGCCTGGTGTCCTATGCAATCGCCGGTGTTCCCAACGACCTGATGGGCGAAGGCCCTA
TCCCGTCGTCCAAGCTGGCCCTGCAGCGCGCGGGCCTGACGCTGGACAAGATCGACCTGATCGAATCCAACGAGGCGTTT
GCCGCGCAGTCGCTGACCGTGGCGAAGGGGCTGGAACTCGACCCGGCAAAGACCAACCCCAATGGCGGCGCCATCGCGCT
CGGCCACCCGGTTGGCGCGACCGGCGCGGTCATCATCACCAAGCTGCTGCACGAACTGCAGCGCACGGGCGGCCGTTACG
GCATGGCGACGATGTGTATTGGCGGTGGCCAAGGCATCACCACGATCTGGGAACGCATCTGA

Upstream 100 bases:

>100_bases
TCGGGCTCGTTTGGCGAGCCCTCGTGGTGTGTCTGCTGATGCTTGCCTTGCTCGGCGTTGCCGGCTGGGTAGGCCGTTGA
ATCTCAAGGAGGGAGTGCAG

Downstream 100 bases:

>100_bases
TCGCTGCAGCAGCGTCGTAAGCAAAAGGCCCGCGAAAGCGGGCCTTTTTTTGTCCACTCGCGGACGTAAGCCGCGCGTAT
CCGAGCGCTACAGGTGGAAG

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MASREVVVLSAVRSAVGGFGGSLKDMEPAELGGVVVKEAISRAGVDPKQVTFATVGNCIPTESRYPYVARVATIQGGMSM
ESVAFAVNRLCGSAQQAIVNSAQAILLGDADYAVGGGVEVMSRGAYLMPALRNGARMGDTKAIDAMVAVLTDPFGVGHMG
ITAENLAAKWGISREEQDAFALESQRRAAEAIADGRFKGQIVPITFETRKGPVVFDTDEHPRATTMEALAKMKPAFKKDG
SVTAGNASGINDAAAFLVLADAAKASAAGHKPMARLVSYAIAGVPNDLMGEGPIPSSKLALQRAGLTLDKIDLIESNEAF
AAQSLTVAKGLELDPAKTNPNGGAIALGHPVGATGAVIITKLLHELQRTGGRYGMATMCIGGGQGITTIWERI

Sequences:

>Translated_393_residues
MASREVVVLSAVRSAVGGFGGSLKDMEPAELGGVVVKEAISRAGVDPKQVTFATVGNCIPTESRYPYVARVATIQGGMSM
ESVAFAVNRLCGSAQQAIVNSAQAILLGDADYAVGGGVEVMSRGAYLMPALRNGARMGDTKAIDAMVAVLTDPFGVGHMG
ITAENLAAKWGISREEQDAFALESQRRAAEAIADGRFKGQIVPITFETRKGPVVFDTDEHPRATTMEALAKMKPAFKKDG
SVTAGNASGINDAAAFLVLADAAKASAAGHKPMARLVSYAIAGVPNDLMGEGPIPSSKLALQRAGLTLDKIDLIESNEAF
AAQSLTVAKGLELDPAKTNPNGGAIALGHPVGATGAVIITKLLHELQRTGGRYGMATMCIGGGQGITTIWERI
>Mature_392_residues
ASREVVVLSAVRSAVGGFGGSLKDMEPAELGGVVVKEAISRAGVDPKQVTFATVGNCIPTESRYPYVARVATIQGGMSME
SVAFAVNRLCGSAQQAIVNSAQAILLGDADYAVGGGVEVMSRGAYLMPALRNGARMGDTKAIDAMVAVLTDPFGVGHMGI
TAENLAAKWGISREEQDAFALESQRRAAEAIADGRFKGQIVPITFETRKGPVVFDTDEHPRATTMEALAKMKPAFKKDGS
VTAGNASGINDAAAFLVLADAAKASAAGHKPMARLVSYAIAGVPNDLMGEGPIPSSKLALQRAGLTLDKIDLIESNEAFA
AQSLTVAKGLELDPAKTNPNGGAIALGHPVGATGAVIITKLLHELQRTGGRYGMATMCIGGGQGITTIWERI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=390, Percent_Identity=44.1025641025641, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=43.1472081218274, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=41.5189873417722, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI4557237, Length=384, Percent_Identity=37.7604166666667, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=32.311320754717, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI194353979, Length=249, Percent_Identity=33.7349397590361, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1788554, Length=395, Percent_Identity=49.3670886075949, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=44.2455242966752, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=42.25, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=39.2059553349876, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=31.5789473684211, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=394, Percent_Identity=43.4010152284264, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25147385, Length=386, Percent_Identity=33.419689119171, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=33.3333333333333, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.4964539007092, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=36.9727047146402, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322031, Length=398, Percent_Identity=34.9246231155779, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=44.1326530612245, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=44.3037974683544, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=35.7868020304569, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=30.1401869158879, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40637; Mature: 40506

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASREVVVLSAVRSAVGGFGGSLKDMEPAELGGVVVKEAISRAGVDPKQVTFATVGNCIP
CCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHHHCCCCC
TESRYPYVARVATIQGGMSMESVAFAVNRLCGSAQQAIVNSAQAILLGDADYAVGGGVEV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
MSRGAYLMPALRNGARMGDTKAIDAMVAVLTDPFGVGHMGITAENLAAKWGISREEQDAF
HHCCCEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHH
ALESQRRAAEAIADGRFKGQIVPITFETRKGPVVFDTDEHPRATTMEALAKMKPAFKKDG
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCHHHCCC
SVTAGNASGINDAAAFLVLADAAKASAAGHKPMARLVSYAIAGVPNDLMGEGPIPSSKLA
CEECCCCCCCCHHHHHHHEEHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHH
LQRAGLTLDKIDLIESNEAFAAQSLTVAKGLELDPAKTNPNGGAIALGHPVGATGAVIIT
HHHCCCCEEHEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHH
KLLHELQRTGGRYGMATMCIGGGQGITTIWERI
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCC
>Mature Secondary Structure 
ASREVVVLSAVRSAVGGFGGSLKDMEPAELGGVVVKEAISRAGVDPKQVTFATVGNCIP
CCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHHHCCCCC
TESRYPYVARVATIQGGMSMESVAFAVNRLCGSAQQAIVNSAQAILLGDADYAVGGGVEV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
MSRGAYLMPALRNGARMGDTKAIDAMVAVLTDPFGVGHMGITAENLAAKWGISREEQDAF
HHCCCEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHH
ALESQRRAAEAIADGRFKGQIVPITFETRKGPVVFDTDEHPRATTMEALAKMKPAFKKDG
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCHHHCCC
SVTAGNASGINDAAAFLVLADAAKASAAGHKPMARLVSYAIAGVPNDLMGEGPIPSSKLA
CEECCCCCCCCHHHHHHHEEHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCHHHHH
LQRAGLTLDKIDLIESNEAFAAQSLTVAKGLELDPAKTNPNGGAIALGHPVGATGAVIIT
HHHCCCCEEHEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHH
KLLHELQRTGGRYGMATMCIGGGQGITTIWERI
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]