Click here to switch to the map view.

The map label for this gene is acsA [H]

Identifier: 119898440

GI number: 119898440

Start: 2351727

End: 2353712

Strand: Reverse

Name: acsA [H]

Synonym: azo2149

Alternate gene names: 119898440

Gene position: 2353712-2351727 (Counterclockwise)

Preceding gene: 119898441

Following gene: 119898439

Centisome position: 53.79

GC content: 65.51

Gene sequence:

>1986_bases
ATGAACACCACCGCCAGCCAACCCGACACCCCGCTTTGGACGCCGTCCGCGGAACGGATCGCAGCCGCCAACATGACCGC
GTTCCGCCATACCGCGGAGCGGCGCTGGAACATCGAGTTGCCGGATTACGCCGCGTTGCACGCGTGGTCGGTCGACCATC
CCGAGCAGTTCTGGGTCAGCGTATGGGAGGACGGCGACGTGGTGGGCAAGCGCGGCGTGCGCGTGCTGGTGGATGGCGAC
CGCATGCCGGGCGCGCAGTGGTTCCCCGACGCGCGGCTCAACTTTGCGCAGAACCTGCTGCGCCGTCGTGACGGTGACGA
TGCGGTGGTGTTCTGGGGCGAGGACAAGGTGCGCGACCGCCTCACCCACGGCGAACTCTACCGGCGCGTGGCGCAGTTCT
CGGCTGCACTGCGCGAACAGGGCGTGGGGAAGGGCGACCGTGTGGCGGCCTACATGCCCAACATGCCGGAGACGCTGATC
GCGATGTTGGCCGCGGCGAGCATCGGCGCCATCTTTACGTCCGCTTCGCCCGATTTCGGCGTGCAGGGCGTGCTCGACCG
CTTCGGCCAGACCGAGCCCAAGGTGTTGCTCGCCTGCGACGGCTATTACTACAACGGCAAGATGGTCGATTGCCTCGCCA
AGCTGGGCGAGATCGTGCCGCAATTGCCTTCGGTGGAGCGGGTGGTGATCGTGCCTTATGTGCACCGCGACCACGCGCTC
GGGGGCATCCCGCATGCCCGGATGTATGCCGATTTTGTCGCCCCGCACCACGCGGCGACCGAGATCGGGTTCGAAGCGCT
GCCGTTCTCCCATCCGCTTTACGTGATGTACTCCTCAGGCACGACCGGGGTTCCGAAGTGCATCGTTCATAGCGCAGGCG
GTGCCCTGCTGCAGCATTTGAAAGAGCACCGCCTGCACTGCGACGTGAAGCCTGGCGACCGCGTGTTCTACTTCACCACG
TGCGGCTGGATGATGTGGAACTGGCTGGTGTCGGGGCTGGCCGCGGGGGCGACCATCCTGCTATACGACGGTTCGCCGTT
CGCGGCCGACAACCGCATCCTGTTCGATTACGCCGACGCGGAACGGATGACCCACTTCGGCACGTCGGCCAAGTTCATCG
ACGCAGCCGCCAAGTTCGGGCTGAAACCGCGCGAAACCCATAGCCTGGCGACGGTGCGGGCAATGATGAGCACCGGCAGT
CCGCTGGTGCCGGAGGGTTTCGACTACGTCTATCGCGACATCAAGGCCGATCTCCAGCTCTCGTCGATTTCGGGCGGAAC
CGACATCCTGTCGTGCTTCGTGCTCGGCAATCCGGTGCTGCCGGTGTGGCGTGGCGAAATCCAGTGCCGCGGCCTCGGAC
TTGCAGTGGATGTATGGGACGACGAAGGCCGGCCGGTGCGGGGCGAGAAGGGCGAACTGGTGTGCGCGCGGCCGTTTCCG
GCGATGCCGGTCGGATTCTGGCGCGACGAAGACGGCAGCAAGTATCGCGCGGCCTATTTCGAACGCTTCGACAACGTCTG
GTGCCACGGCGATTTCTGCGAGATCACCGCGCATGGCGGTCTCATCATCTACGGTCGCTCCGATGCCACCCTCAACCCGG
GCGGCGTTCGCATCGGCACTGCCGAGATCTACCGCCAGGTCGAGAAGTTGCATGAAGTGGTCGAATCCATCGTGATCGGG
CAGGACTGGCCGCCGCAGAACCCCAACGACGTGCGCGTGGTGCTGTTCGTCAAGCTGCGCGATGGCATGACGCTCGACGA
CACGCTGGCGGACCGCATCAAGCGCACCATCCGCGACAACACCACCCCGCGCCACGTGCCGGCCAAGGTGCTGCAGGTGG
CCGATATTCCACGTACCAAGAGCGGCAAGATCGTGGAACTGGCGGTGCGTAACGTGGTGCATGGCCGGGCGGTGAAGAAC
CAGGAGGCGCTGGCCAACCCCGAAGCGCTCGCGCACTTCCGCGACCGCGACGAACTGGCCGGTTAA

Upstream 100 bases:

>100_bases
GGATGGACACCGCGACTGGCAAGTCCGTACCCTTGCCGGATACACTGCGGGCTTCTGTAAGTTAGGTATTAACAAATAAA
TTAAACAAGAGGAGACAGGC

Downstream 100 bases:

>100_bases
TCGGTCCGCACAAGGCGGAAGTCCGTTTCCGCCCGCATCGCGCGAACCCGCGCGGGGGATCACAGTCGTACCTTGCATTG
CGGCGGCCCGACGGGGTGTG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MNTTASQPDTPLWTPSAERIAAANMTAFRHTAERRWNIELPDYAALHAWSVDHPEQFWVSVWEDGDVVGKRGVRVLVDGD
RMPGAQWFPDARLNFAQNLLRRRDGDDAVVFWGEDKVRDRLTHGELYRRVAQFSAALREQGVGKGDRVAAYMPNMPETLI
AMLAAASIGAIFTSASPDFGVQGVLDRFGQTEPKVLLACDGYYYNGKMVDCLAKLGEIVPQLPSVERVVIVPYVHRDHAL
GGIPHARMYADFVAPHHAATEIGFEALPFSHPLYVMYSSGTTGVPKCIVHSAGGALLQHLKEHRLHCDVKPGDRVFYFTT
CGWMMWNWLVSGLAAGATILLYDGSPFAADNRILFDYADAERMTHFGTSAKFIDAAAKFGLKPRETHSLATVRAMMSTGS
PLVPEGFDYVYRDIKADLQLSSISGGTDILSCFVLGNPVLPVWRGEIQCRGLGLAVDVWDDEGRPVRGEKGELVCARPFP
AMPVGFWRDEDGSKYRAAYFERFDNVWCHGDFCEITAHGGLIIYGRSDATLNPGGVRIGTAEIYRQVEKLHEVVESIVIG
QDWPPQNPNDVRVVLFVKLRDGMTLDDTLADRIKRTIRDNTTPRHVPAKVLQVADIPRTKSGKIVELAVRNVVHGRAVKN
QEALANPEALAHFRDRDELAG

Sequences:

>Translated_661_residues
MNTTASQPDTPLWTPSAERIAAANMTAFRHTAERRWNIELPDYAALHAWSVDHPEQFWVSVWEDGDVVGKRGVRVLVDGD
RMPGAQWFPDARLNFAQNLLRRRDGDDAVVFWGEDKVRDRLTHGELYRRVAQFSAALREQGVGKGDRVAAYMPNMPETLI
AMLAAASIGAIFTSASPDFGVQGVLDRFGQTEPKVLLACDGYYYNGKMVDCLAKLGEIVPQLPSVERVVIVPYVHRDHAL
GGIPHARMYADFVAPHHAATEIGFEALPFSHPLYVMYSSGTTGVPKCIVHSAGGALLQHLKEHRLHCDVKPGDRVFYFTT
CGWMMWNWLVSGLAAGATILLYDGSPFAADNRILFDYADAERMTHFGTSAKFIDAAAKFGLKPRETHSLATVRAMMSTGS
PLVPEGFDYVYRDIKADLQLSSISGGTDILSCFVLGNPVLPVWRGEIQCRGLGLAVDVWDDEGRPVRGEKGELVCARPFP
AMPVGFWRDEDGSKYRAAYFERFDNVWCHGDFCEITAHGGLIIYGRSDATLNPGGVRIGTAEIYRQVEKLHEVVESIVIG
QDWPPQNPNDVRVVLFVKLRDGMTLDDTLADRIKRTIRDNTTPRHVPAKVLQVADIPRTKSGKIVELAVRNVVHGRAVKN
QEALANPEALAHFRDRDELAG
>Mature_661_residues
MNTTASQPDTPLWTPSAERIAAANMTAFRHTAERRWNIELPDYAALHAWSVDHPEQFWVSVWEDGDVVGKRGVRVLVDGD
RMPGAQWFPDARLNFAQNLLRRRDGDDAVVFWGEDKVRDRLTHGELYRRVAQFSAALREQGVGKGDRVAAYMPNMPETLI
AMLAAASIGAIFTSASPDFGVQGVLDRFGQTEPKVLLACDGYYYNGKMVDCLAKLGEIVPQLPSVERVVIVPYVHRDHAL
GGIPHARMYADFVAPHHAATEIGFEALPFSHPLYVMYSSGTTGVPKCIVHSAGGALLQHLKEHRLHCDVKPGDRVFYFTT
CGWMMWNWLVSGLAAGATILLYDGSPFAADNRILFDYADAERMTHFGTSAKFIDAAAKFGLKPRETHSLATVRAMMSTGS
PLVPEGFDYVYRDIKADLQLSSISGGTDILSCFVLGNPVLPVWRGEIQCRGLGLAVDVWDDEGRPVRGEKGELVCARPFP
AMPVGFWRDEDGSKYRAAYFERFDNVWCHGDFCEITAHGGLIIYGRSDATLNPGGVRIGTAEIYRQVEKLHEVVESIVIG
QDWPPQNPNDVRVVLFVKLRDGMTLDDTLADRIKRTIRDNTTPRHVPAKVLQVADIPRTKSGKIVELAVRNVVHGRAVKN
QEALANPEALAHFRDRDELAG

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=659, Percent_Identity=44.9165402124431, Blast_Score=589, Evalue=1e-168,
Organism=Homo sapiens, GI8923896, Length=664, Percent_Identity=27.1084337349398, Blast_Score=211, Evalue=3e-54,
Organism=Homo sapiens, GI253970489, Length=677, Percent_Identity=26.8833087149188, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI28416953, Length=637, Percent_Identity=26.0596546310832, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI13375727, Length=645, Percent_Identity=24.4961240310078, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=22.7099236641221, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1790505, Length=638, Percent_Identity=26.1755485893417, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786529, Length=653, Percent_Identity=22.9709035222052, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17569507, Length=644, Percent_Identity=38.9751552795031, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71983001, Length=634, Percent_Identity=25.0788643533123, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71982997, Length=629, Percent_Identity=25.4372019077901, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17560308, Length=578, Percent_Identity=23.7024221453287, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323182, Length=652, Percent_Identity=25.6134969325153, Blast_Score=187, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6319264, Length=659, Percent_Identity=26.7071320182094, Blast_Score=180, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=27.8461538461538, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI62472339, Length=555, Percent_Identity=28.1081081081081, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24667959, Length=524, Percent_Identity=27.2900763358779, Blast_Score=177, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21355225, Length=628, Percent_Identity=24.3630573248408, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 73317; Mature: 73317

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTASQPDTPLWTPSAERIAAANMTAFRHTAERRWNIELPDYAALHAWSVDHPEQFWVS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHEEE
VWEDGDVVGKRGVRVLVDGDRMPGAQWFPDARLNFAQNLLRRRDGDDAVVFWGEDKVRDR
EECCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
LTHGELYRRVAQFSAALREQGVGKGDRVAAYMPNMPETLIAMLAAASIGAIFTSASPDFG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCC
VQGVLDRFGQTEPKVLLACDGYYYNGKMVDCLAKLGEIVPQLPSVERVVIVPYVHRDHAL
HHHHHHHCCCCCCCEEEEECCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCC
GGIPHARMYADFVAPHHAATEIGFEALPFSHPLYVMYSSGTTGVPKCIVHSAGGALLQHL
CCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHH
KEHRLHCDVKPGDRVFYFTTCGWMMWNWLVSGLAAGATILLYDGSPFAADNRILFDYADA
HHCCCEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCH
ERMTHFGTSAKFIDAAAKFGLKPRETHSLATVRAMMSTGSPLVPEGFDYVYRDIKADLQL
HHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEE
SSISGGTDILSCFVLGNPVLPVWRGEIQCRGLGLAVDVWDDEGRPVRGEKGELVCARPFP
ECCCCCHHHHHHHHHCCCCCEEECCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEECCCC
AMPVGFWRDEDGSKYRAAYFERFDNVWCHGDFCEITAHGGLIIYGRSDATLNPGGVRIGT
CCCCCCEECCCCCHHHHHHHHHHCCEEECCCEEEEEECCCEEEECCCCCEECCCCEEECH
AEIYRQVEKLHEVVESIVIGQDWPPQNPNDVRVVLFVKLRDGMTLDDTLADRIKRTIRDN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
TTPRHVPAKVLQVADIPRTKSGKIVELAVRNVVHGRAVKNQEALANPEALAHFRDRDELA
CCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCCHHHHCCHHHHHHHCCHHHCC
G
C
>Mature Secondary Structure
MNTTASQPDTPLWTPSAERIAAANMTAFRHTAERRWNIELPDYAALHAWSVDHPEQFWVS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHEEE
VWEDGDVVGKRGVRVLVDGDRMPGAQWFPDARLNFAQNLLRRRDGDDAVVFWGEDKVRDR
EECCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
LTHGELYRRVAQFSAALREQGVGKGDRVAAYMPNMPETLIAMLAAASIGAIFTSASPDFG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCC
VQGVLDRFGQTEPKVLLACDGYYYNGKMVDCLAKLGEIVPQLPSVERVVIVPYVHRDHAL
HHHHHHHCCCCCCCEEEEECCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCC
GGIPHARMYADFVAPHHAATEIGFEALPFSHPLYVMYSSGTTGVPKCIVHSAGGALLQHL
CCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHH
KEHRLHCDVKPGDRVFYFTTCGWMMWNWLVSGLAAGATILLYDGSPFAADNRILFDYADA
HHCCCEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCH
ERMTHFGTSAKFIDAAAKFGLKPRETHSLATVRAMMSTGSPLVPEGFDYVYRDIKADLQL
HHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEE
SSISGGTDILSCFVLGNPVLPVWRGEIQCRGLGLAVDVWDDEGRPVRGEKGELVCARPFP
ECCCCCHHHHHHHHHCCCCCEEECCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEECCCC
AMPVGFWRDEDGSKYRAAYFERFDNVWCHGDFCEITAHGGLIIYGRSDATLNPGGVRIGT
CCCCCCEECCCCCHHHHHHHHHHCCEEECCCEEEEEECCCEEEECCCCCEECCCCEEECH
AEIYRQVEKLHEVVESIVIGQDWPPQNPNDVRVVLFVKLRDGMTLDDTLADRIKRTIRDN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
TTPRHVPAKVLQVADIPRTKSGKIVELAVRNVVHGRAVKNQEALANPEALAHFRDRDELA
CCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCCHHHHCCHHHHHHHCCHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]