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The map label for this gene is eno [H]

Identifier: 119898435

GI number: 119898435

Start: 2345585

End: 2346874

Strand: Reverse

Name: eno [H]

Synonym: azo2144

Alternate gene names: 119898435

Gene position: 2346874-2345585 (Counterclockwise)

Preceding gene: 119898436

Following gene: 119898434

Centisome position: 53.63

GC content: 63.95

Gene sequence:

>1290_bases
ATGAGTGCAATCGTTGACGTGATCGCCCGCGAGATTCTGGACTCCCGCGGCAATCCCACTGTCGAAGCCGACGTGCTGCT
TGAGTCCGGGGTGATGGGGCGTGCTGCCGTGCCCTCCGGCGCCTCCACCGGTTCCCGCGAAGCCATCGAACTGCGCGACG
GCGACGCCGCACGCTTCCTCGGCAAGGGCGTGCTGCGCGCCGTCGAGAACGTCAACACCGAGATTTCCGAAGCGATCATC
GGTCTGGACGCCGAAGAGCAGGCCTTCATCGACCGCACCCTGATCGACCTGGACGGCACCGAGAACAAGTCGCGTCTCGG
CGCCAATGCGACGCTGGCAGTGTCGATGGCGGTGGCACGCGCCGCGGCCGAGGAGGCCGGCCTGCCGCTGTACCGCTATT
TCGGCGGTTCGGGCCCGATGGCCATGCCGGTCCCGATGATGAACGTCATCAACGGCGGCGAGCACGCCAACAACAGCCTG
GACATCCAGGAATGCATGATCATGCCGGTGAGCATGGGCAGCTTCCGTGAGGCCCTGCGTTGTGGCGCGGAGATCTTCCA
TCACCTGAAGAAGATCACCGACAAGAAGGGCTACCCGACCACTGTCGGTGATGAAGGCGGTTTCGCGCCCAACGTGTCCG
GCACCGAAGAAGCGCTCAACCTGATCCAGGAAGCCATCGCCGCCGCCGGTTACGAGCCGGGCCGCGACGTGCTGCTGGCG
CTCGACTGCGCTGCGTCCGAGTTCTACAAGGACGGCAAGTACGAGCTGAAGGGCGAAGGCCTCAGCCTCACCTCCGAAGG
CTTTACCGACTACCTCGCCACCTTGGCCGACAAGTTCCCGATCGTGTCGATCGAGGACGGCATGGCCGAAGGCGACTGGG
CCGGCTGGAAGCACCTCACCGACCGCCTCGGCCGCAAGATTCAGTTGGTCGGCGACGATCTCTTCGTTACCAACACCAAG
ATCCTCGAGCAGGGCATCGACCAGGGCGTGGCCAACTCCATCCTGATCAAGATCAACCAGATCGGCACCCTGTCGGAAAC
CTTCGCGGCGGTTGAAATGGCCAAGCGCGCCGGTTACACCGCGGTGATCTCGCACCGTTCGGGTGAAACCGAAGACTCCA
CCATCGCCGACATCGCGGTGGGCCTCAACGCGATGCAGATCAAGACCGGTTCGCTGTCGCGCTCGGACCGTATCGCCAAG
TACAACCAGCTGCTCCGCATCGAGGAAGACCTCGGCAACACCGTGGTGTATCCGGGCAAGCGCGCGTTCTACAACCTGCG
CAGCCGCTAA

Upstream 100 bases:

>100_bases
GGAAACCGAGGCCTGATCCGCTTCGTGTGCCGCCCCATGTGCAGGCGCACCCTGATTTTTTCTTCCAATTCCTAATCCGT
AACCGAAACAGGAACATTCC

Downstream 100 bases:

>100_bases
GCGACGCCGGTTCGCTTTCCGCTTCGACTGCCGCGACATCTGCCGCGGCAGTCGATTGCGTCTTTCTTTCCCTAGCCGTC
GAACTGTTCCATGCGTTGGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGSREAIELRDGDAARFLGKGVLRAVENVNTEISEAII
GLDAEEQAFIDRTLIDLDGTENKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGPMAMPVPMMNVINGGEHANNSL
DIQECMIMPVSMGSFREALRCGAEIFHHLKKITDKKGYPTTVGDEGGFAPNVSGTEEALNLIQEAIAAAGYEPGRDVLLA
LDCAASEFYKDGKYELKGEGLSLTSEGFTDYLATLADKFPIVSIEDGMAEGDWAGWKHLTDRLGRKIQLVGDDLFVTNTK
ILEQGIDQGVANSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAMQIKTGSLSRSDRIAK
YNQLLRIEEDLGNTVVYPGKRAFYNLRSR

Sequences:

>Translated_429_residues
MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGSREAIELRDGDAARFLGKGVLRAVENVNTEISEAII
GLDAEEQAFIDRTLIDLDGTENKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGPMAMPVPMMNVINGGEHANNSL
DIQECMIMPVSMGSFREALRCGAEIFHHLKKITDKKGYPTTVGDEGGFAPNVSGTEEALNLIQEAIAAAGYEPGRDVLLA
LDCAASEFYKDGKYELKGEGLSLTSEGFTDYLATLADKFPIVSIEDGMAEGDWAGWKHLTDRLGRKIQLVGDDLFVTNTK
ILEQGIDQGVANSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAMQIKTGSLSRSDRIAK
YNQLLRIEEDLGNTVVYPGKRAFYNLRSR
>Mature_428_residues
SAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGSREAIELRDGDAARFLGKGVLRAVENVNTEISEAIIG
LDAEEQAFIDRTLIDLDGTENKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGPMAMPVPMMNVINGGEHANNSLD
IQECMIMPVSMGSFREALRCGAEIFHHLKKITDKKGYPTTVGDEGGFAPNVSGTEEALNLIQEAIAAAGYEPGRDVLLAL
DCAASEFYKDGKYELKGEGLSLTSEGFTDYLATLADKFPIVSIEDGMAEGDWAGWKHLTDRLGRKIQLVGDDLFVTNTKI
LEQGIDQGVANSILIKINQIGTLSETFAAVEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAMQIKTGSLSRSDRIAKY
NQLLRIEEDLGNTVVYPGKRAFYNLRSR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=439, Percent_Identity=50.1138952164009, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=439, Percent_Identity=50.1138952164009, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI5803011, Length=438, Percent_Identity=51.5981735159817, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=48.5057471264368, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=437, Percent_Identity=45.5377574370709, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310129182, Length=222, Percent_Identity=27.4774774774775, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310110045, Length=222, Percent_Identity=27.4774774774775, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310120572, Length=222, Percent_Identity=27.4774774774775, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=65.4929577464789, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=50.9132420091324, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=50.9132420091324, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=198, Percent_Identity=44.9494949494949, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.8845265588915, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=49.0783410138249, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=49.0783410138249, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=48.8479262672811, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.6535796766744, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=51.5222482435597, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=51.5222482435597, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=51.5222482435597, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=51.5222482435597, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=427, Percent_Identity=51.5222482435597, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=427, Percent_Identity=51.5222482435597, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45947; Mature: 45815

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGSREAIELRDGDAARFL
CHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVLRAVENVNTEISEAIIGLDAEEQAFIDRTLIDLDGTENKSRLGANATLAVSMAVAR
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGPMAMPVPMMNVINGGEHANNSLDIQECMIMPVSMGSFREALR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHEECCCHHHHHHHHH
CGAEIFHHLKKITDKKGYPTTVGDEGGFAPNVSGTEEALNLIQEAIAAAGYEPGRDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYKDGKYELKGEGLSLTSEGFTDYLATLADKFPIVSIEDGMAEGDWAGWKHLT
EEHHHHHHHCCCCEEECCCCCEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
DRLGRKIQLVGDDLFVTNTKILEQGIDQGVANSILIKINQIGTLSETFAAVEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAMQIKTGSLSRSDRIAKYNQLLRIEEDLGNTVVYPGK
EEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCH
RAFYNLRSR
HHHHCCCCC
>Mature Secondary Structure 
SAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTGSREAIELRDGDAARFL
HHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVLRAVENVNTEISEAIIGLDAEEQAFIDRTLIDLDGTENKSRLGANATLAVSMAVAR
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGPMAMPVPMMNVINGGEHANNSLDIQECMIMPVSMGSFREALR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHEECCCHHHHHHHHH
CGAEIFHHLKKITDKKGYPTTVGDEGGFAPNVSGTEEALNLIQEAIAAAGYEPGRDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYKDGKYELKGEGLSLTSEGFTDYLATLADKFPIVSIEDGMAEGDWAGWKHLT
EEHHHHHHHCCCCEEECCCCCEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
DRLGRKIQLVGDDLFVTNTKILEQGIDQGVANSILIKINQIGTLSETFAAVEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAMQIKTGSLSRSDRIAKYNQLLRIEEDLGNTVVYPGK
EEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCH
RAFYNLRSR
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA