Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 119898401

GI number: 119898401

Start: 2317918

End: 2318988

Strand: Reverse

Name: rluB [H]

Synonym: azo2110

Alternate gene names: 119898401

Gene position: 2318988-2317918 (Counterclockwise)

Preceding gene: 119898402

Following gene: 119898400

Centisome position: 52.99

GC content: 69.56

Gene sequence:

>1071_bases
TTGTCGACACCTCGCAAATCGAATCGCCCGCTGCGCCCGCCCCAGAAGAAGAGCCGGGGGCCTGAGGGCGCCGCCGACAC
GCGCCGGGCCGGTGCGCCCGCCGCCGTACGCGGCGAACCGCGCCGGCGCGCGCCAGAGCAGGTCAGCGATGATCCGCAAC
GACGCCCGGACGCGCACCGATCGGCGCTCGGCAGCCCGCAGGATGCCGGTGAGTCCGCGCGCCGCGGTGAGCGACGTCCC
GCGCCTGCCGCCCCCATTTTTGCCGAACCCGAGCGCCTGCAGAAGGTACTTGCCCAAGCCGGCGTCGCCTCGCGCCGCGA
GATCGAGGAGCTGGTGGTCGCCGGGCGGATTTCCGTCAATGGCCTGCCCGCGTCGCTGGGGCAGAAGATTGGCCCGGGCG
ACCGGGTCAAGCTCAACGGCAAGTTGATTCCGCTGCGCTTCTCCCAGCGCTCGCCCCGCGTGCTGGTCTACCACAAGCCG
GAAGGCGAGATTGTGTCGCGCGACGACCCGGAGGGCCGGCCGACGGTATTCGAACGCCTTCCGCTGTTGCGCAAGGGGCG
CTGGCTTGCGGTCGGACGCCTCGACTTCAATACCTCGGGTTTGCTGCTGTTTACCAACGACGGCGATCTGGCCAACCGGC
TGATGCACCCGCGTTACGAGCTGACGCGCGAGTACGCAGTGCGGCTGCTGGGTCAGCTGACCGAGGAGCAGGGGCAGTCG
CTGGTGGATGGTATTCAGCTCGAAGACGGCCCGGCGCGTTTCCTGTCGCTCGTCGATGCCGGAGGCGAAGGGGTCAATCA
CTGGTATCGCGTCACCTTGGCGGAAGGTCGTAACCGTGAGGTGCGCCGGATGTTCGAGGCAGTGGGCCTGACCGTGAGCC
GGCTGATGCGCGTCCGTTACGGCCCGGTGGAACTGCCGGCCCGTCTGAAGCGGGGAATGTGGATGGAGTTGCCCGAAGCG
GATGCGTGCGCGCTTGCCGGACTGCCGCCGCCGCGCGTCGATCCGTCGGCGCGAGGGGCGCGTCGGGAGAAGGCGCCGCG
CTTGCATCACACCCTGCCGCACGAACGCTGA

Upstream 100 bases:

>100_bases
CCGGCATTGTTCGCGACCACCCGCCGCTTCCTGGACGACATGGGCCTGCGCAGCCTCACCGAGCTGCCTGCGCTGGCCGA
AATCGAAAGGATCATGGACC

Downstream 100 bases:

>100_bases
ACACCCTTGCCGAGCCATGCGCCGGGCCGCCGTCCTTTCCGGGCGGTTTGCCCGGCTGGGCGTTTTGACGCTATACTTCA
AAGGCTTTGACAGGCGCCGA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSTPRKSNRPLRPPQKKSRGPEGAADTRRAGAPAAVRGEPRRRAPEQVSDDPQRRPDAHRSALGSPQDAGESARRGERRP
APAAPIFAEPERLQKVLAQAGVASRREIEELVVAGRISVNGLPASLGQKIGPGDRVKLNGKLIPLRFSQRSPRVLVYHKP
EGEIVSRDDPEGRPTVFERLPLLRKGRWLAVGRLDFNTSGLLLFTNDGDLANRLMHPRYELTREYAVRLLGQLTEEQGQS
LVDGIQLEDGPARFLSLVDAGGEGVNHWYRVTLAEGRNREVRRMFEAVGLTVSRLMRVRYGPVELPARLKRGMWMELPEA
DACALAGLPPPRVDPSARGARREKAPRLHHTLPHER

Sequences:

>Translated_356_residues
MSTPRKSNRPLRPPQKKSRGPEGAADTRRAGAPAAVRGEPRRRAPEQVSDDPQRRPDAHRSALGSPQDAGESARRGERRP
APAAPIFAEPERLQKVLAQAGVASRREIEELVVAGRISVNGLPASLGQKIGPGDRVKLNGKLIPLRFSQRSPRVLVYHKP
EGEIVSRDDPEGRPTVFERLPLLRKGRWLAVGRLDFNTSGLLLFTNDGDLANRLMHPRYELTREYAVRLLGQLTEEQGQS
LVDGIQLEDGPARFLSLVDAGGEGVNHWYRVTLAEGRNREVRRMFEAVGLTVSRLMRVRYGPVELPARLKRGMWMELPEA
DACALAGLPPPRVDPSARGARREKAPRLHHTLPHER
>Mature_355_residues
STPRKSNRPLRPPQKKSRGPEGAADTRRAGAPAAVRGEPRRRAPEQVSDDPQRRPDAHRSALGSPQDAGESARRGERRPA
PAAPIFAEPERLQKVLAQAGVASRREIEELVVAGRISVNGLPASLGQKIGPGDRVKLNGKLIPLRFSQRSPRVLVYHKPE
GEIVSRDDPEGRPTVFERLPLLRKGRWLAVGRLDFNTSGLLLFTNDGDLANRLMHPRYELTREYAVRLLGQLTEEQGQSL
VDGIQLEDGPARFLSLVDAGGEGVNHWYRVTLAEGRNREVRRMFEAVGLTVSRLMRVRYGPVELPARLKRGMWMELPEAD
ACALAGLPPPRVDPSARGARREKAPRLHHTLPHER

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=58.9430894308943, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=31.2236286919831, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=31.5352697095436, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=33.6898395721925, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 39548; Mature: 39417

Theoretical pI: Translated: 11.37; Mature: 11.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPRKSNRPLRPPQKKSRGPEGAADTRRAGAPAAVRGEPRRRAPEQVSDDPQRRPDAHR
CCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCHHH
SALGSPQDAGESARRGERRPAPAAPIFAEPERLQKVLAQAGVASRREIEELVVAGRISVN
HHCCCCHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEC
GLPASLGQKIGPGDRVKLNGKLIPLRFSQRSPRVLVYHKPEGEIVSRDDPEGRPTVFERL
CCCHHHHCCCCCCCEEEECCEEEEEEECCCCCEEEEEECCCCCEECCCCCCCCCHHHHHC
PLLRKGRWLAVGRLDFNTSGLLLFTNDGDLANRLMHPRYELTREYAVRLLGQLTEEQGQS
CHHHCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVDGIQLEDGPARFLSLVDAGGEGVNHWYRVTLAEGRNREVRRMFEAVGLTVSRLMRVRY
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GPVELPARLKRGMWMELPEADACALAGLPPPRVDPSARGARREKAPRLHHTLPHER
CCCCHHHHHHCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHCCCCHHCCCCCCC
>Mature Secondary Structure 
STPRKSNRPLRPPQKKSRGPEGAADTRRAGAPAAVRGEPRRRAPEQVSDDPQRRPDAHR
CCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCHHH
SALGSPQDAGESARRGERRPAPAAPIFAEPERLQKVLAQAGVASRREIEELVVAGRISVN
HHCCCCHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEC
GLPASLGQKIGPGDRVKLNGKLIPLRFSQRSPRVLVYHKPEGEIVSRDDPEGRPTVFERL
CCCHHHHCCCCCCCEEEECCEEEEEEECCCCCEEEEEECCCCCEECCCCCCCCCHHHHHC
PLLRKGRWLAVGRLDFNTSGLLLFTNDGDLANRLMHPRYELTREYAVRLLGQLTEEQGQS
CHHHCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVDGIQLEDGPARFLSLVDAGGEGVNHWYRVTLAEGRNREVRRMFEAVGLTVSRLMRVRY
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GPVELPARLKRGMWMELPEADACALAGLPPPRVDPSARGARREKAPRLHHTLPHER
CCCCHHHHHHCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]