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The map label for this gene is gspE2 [H]

Identifier: 119898386

GI number: 119898386

Start: 2299696

End: 2301372

Strand: Reverse

Name: gspE2 [H]

Synonym: azo2095

Alternate gene names: 119898386

Gene position: 2301372-2299696 (Counterclockwise)

Preceding gene: 119898387

Following gene: 119898385

Centisome position: 52.59

GC content: 69.17

Gene sequence:

>1677_bases
ATGAACGCGCCGGTCGACCTTCATCCGCGGTCGGCGCCGGCGTTTTCCGCCGACGAGCTGGCGCGCGCCTCCGCCGCAGG
AGGGCGCCTGCTCGACGGCCTCGCCACTGTGGAAGCCGACCCTGCTGAACGCCTGCGGCGTCTGGCCGCCACCTGCGGCC
TGAACCCGATCCATCACGCCCGGCTGATGTCGCTGACGCCGGATTTCGGCGCCGTCCGTTTCGAGACCGCGCTGCAGCGC
GAATGCGTCGCCCTGCGCGACGAAGACGGCGGGCTGGTGGTGGTCATCGCCGATCCCTTCGACAACGATCTGCTCGACGG
TCTCGCGGCGCGGCTCGGCGAAGGCTTCACGAGCGCGGTGGGCGACCGCGAGGACATTGCCGGCTACCTTGCGCGCCACG
AGGACGAAGTGAGGCGTGCGGCCGGACTGCAACTGGGCGGCGACGACGCGCAGCAGGACGACGGCGCGGAAGATCTGTCG
CTGCGCCGGATCAGCAGTGATGCGAGTCCGGTCGTCAAACTGGTCAACTCCACGCTCTACGATGCCCTCAAGCAAGGCGC
GAGCGACATCCACCTCGAAGCCGTACCCGGCGGGCTGGTGATCAAGTACCGGGTGGACGGCGTGCTGGCGCGGGTCGGCG
GTGCCAACGGCGCGGAGCTGGCGGAGCAGGCGATCTCCCGCATCAAGGTGATGGCCGAACTGGATATCGCGGAGCGCCGG
GTGCCGCAGGATGGCCGCTTCAAGGCCCAGGCCGGCGGGCGCGAGATCGACTTCCGCGTGTCCATCATGCCCAGCATCCA
CGGCGAGGACGCAGTGCTGCGCGTGCTCGACAAGGCCCACCTCACCGAGGAGATGCGCGGCCTCACGCTCGAATCGCTCG
GCTTCGCGGCCGATACCCGCGCGGTGCTGCGCCGGCTGGCGGGCGAGCCCTACGGCATGCTGCTGGTCACCGGCCCCACC
GGCAGCGGCAAGACCACGACGCTGTACGCCACGCTGGGCGAGACCAACCACGGTCTGGACAAGATCATCACGATCGAGGA
CCCAGTGGAATACCAGCTGCCCGGCGTGCTGCAGATTCCGGTGAACGAGAAAAAGGGGCTGACGTTCGCGCGCGGGCTGC
GCTCCATCCTGCGCCATGACCCGGACAAGATCATGGTCGGCGAGATCCGCGACGGCGAAACCGCCGAGATCGCGATCCAG
GCGGCGCTGACGGGCCACCTCGTGTTCACCACGGTGCACGCGAACAACGTCTTCGACGTGATCGGCCGCTTCATGCACAT
GGGCATCGACCCCTACAACCTGGTCGCCGCGCTCAACGGCGTGGTGGCGCAGCGGCTGGTGCGGCTCAACTGCCCGTCTT
GCAGCGAGGACATCGCGCCGCCCGCCAGCGAGGTGGAGGCCGCACGGCTGGGAGACGCCAGCGTCTTCCGCTTCCGCGCT
GGACACGGTTGCGGGCACTGCCGCGGTTCCGGCTACCGGGGACGGCGCGCGGTGGCGGAGGTTCTGCTGCTGGACGACGA
GATCCGCGAACTGATCCTCACCCGCGCGCCGATCCGCAGCCTGAAGGAAGCGGCGCGGCGACGCGGCTTCCGCAGCCTGC
GCGAAGCAGTCATCGACATGGTCGCCACCGGCGACACCACCTTGCAGGAACTCAATCGTGTCACGCTTCTCGGCTGA

Upstream 100 bases:

>100_bases
GCCTTTTCGGCCCCTTGCTGATGCTCTTCATCGGCTGCGCGATCGGCCTGATCGTGGTGCTGATGTACCTGCCGATCTTT
CAGCTCGCGGAGACGCTGGG

Downstream 100 bases:

>100_bases
GTCGTGGCGGCTGCTGCTCGGCCGCGACCACTGGCATTTTGACCGCGCTGGCGAGGTACAGCGCGTGCCTGCGGGCGCCG
ACCCGGCCGCCGCGCTTGCC

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MNAPVDLHPRSAPAFSADELARASAAGGRLLDGLATVEADPAERLRRLAATCGLNPIHHARLMSLTPDFGAVRFETALQR
ECVALRDEDGGLVVVIADPFDNDLLDGLAARLGEGFTSAVGDREDIAGYLARHEDEVRRAAGLQLGGDDAQQDDGAEDLS
LRRISSDASPVVKLVNSTLYDALKQGASDIHLEAVPGGLVIKYRVDGVLARVGGANGAELAEQAISRIKVMAELDIAERR
VPQDGRFKAQAGGREIDFRVSIMPSIHGEDAVLRVLDKAHLTEEMRGLTLESLGFAADTRAVLRRLAGEPYGMLLVTGPT
GSGKTTTLYATLGETNHGLDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDGETAEIAIQ
AALTGHLVFTTVHANNVFDVIGRFMHMGIDPYNLVAALNGVVAQRLVRLNCPSCSEDIAPPASEVEAARLGDASVFRFRA
GHGCGHCRGSGYRGRRAVAEVLLLDDEIRELILTRAPIRSLKEAARRRGFRSLREAVIDMVATGDTTLQELNRVTLLG

Sequences:

>Translated_558_residues
MNAPVDLHPRSAPAFSADELARASAAGGRLLDGLATVEADPAERLRRLAATCGLNPIHHARLMSLTPDFGAVRFETALQR
ECVALRDEDGGLVVVIADPFDNDLLDGLAARLGEGFTSAVGDREDIAGYLARHEDEVRRAAGLQLGGDDAQQDDGAEDLS
LRRISSDASPVVKLVNSTLYDALKQGASDIHLEAVPGGLVIKYRVDGVLARVGGANGAELAEQAISRIKVMAELDIAERR
VPQDGRFKAQAGGREIDFRVSIMPSIHGEDAVLRVLDKAHLTEEMRGLTLESLGFAADTRAVLRRLAGEPYGMLLVTGPT
GSGKTTTLYATLGETNHGLDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDGETAEIAIQ
AALTGHLVFTTVHANNVFDVIGRFMHMGIDPYNLVAALNGVVAQRLVRLNCPSCSEDIAPPASEVEAARLGDASVFRFRA
GHGCGHCRGSGYRGRRAVAEVLLLDDEIRELILTRAPIRSLKEAARRRGFRSLREAVIDMVATGDTTLQELNRVTLLG
>Mature_558_residues
MNAPVDLHPRSAPAFSADELARASAAGGRLLDGLATVEADPAERLRRLAATCGLNPIHHARLMSLTPDFGAVRFETALQR
ECVALRDEDGGLVVVIADPFDNDLLDGLAARLGEGFTSAVGDREDIAGYLARHEDEVRRAAGLQLGGDDAQQDDGAEDLS
LRRISSDASPVVKLVNSTLYDALKQGASDIHLEAVPGGLVIKYRVDGVLARVGGANGAELAEQAISRIKVMAELDIAERR
VPQDGRFKAQAGGREIDFRVSIMPSIHGEDAVLRVLDKAHLTEEMRGLTLESLGFAADTRAVLRRLAGEPYGMLLVTGPT
GSGKTTTLYATLGETNHGLDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDGETAEIAIQ
AALTGHLVFTTVHANNVFDVIGRFMHMGIDPYNLVAALNGVVAQRLVRLNCPSCSEDIAPPASEVEAARLGDASVFRFRA
GHGCGHCRGSGYRGRRAVAEVLLLDDEIRELILTRAPIRSLKEAARRRGFRSLREAVIDMVATGDTTLQELNRVTLLG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=387, Percent_Identity=45.4780361757106, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1786296, Length=464, Percent_Identity=36.8534482758621, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI87082188, Length=199, Percent_Identity=40.2010050251256, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59986; Mature: 59986

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVDLHPRSAPAFSADELARASAAGGRLLDGLATVEADPAERLRRLAATCGLNPIHHA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
RLMSLTPDFGAVRFETALQRECVALRDEDGGLVVVIADPFDNDLLDGLAARLGEGFTSAV
HHEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHC
GDREDIAGYLARHEDEVRRAAGLQLGGDDAQQDDGAEDLSLRRISSDASPVVKLVNSTLY
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
DALKQGASDIHLEAVPGGLVIKYRVDGVLARVGGANGAELAEQAISRIKVMAELDIAERR
HHHHCCCCCEEEEECCCCEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPQDGRFKAQAGGREIDFRVSIMPSIHGEDAVLRVLDKAHLTEEMRGLTLESLGFAADTR
CCCCCCEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHH
AVLRRLAGEPYGMLLVTGPTGSGKTTTLYATLGETNHGLDKIITIEDPVEYQLPGVLQIP
HHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEC
VNEKKGLTFARGLRSILRHDPDKIMVGEIRDGETAEIAIQAALTGHLVFTTVHANNVFDV
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEECEEEEEEEECCCHHHH
IGRFMHMGIDPYNLVAALNGVVAQRLVRLNCPSCSEDIAPPASEVEAARLGDASVFRFRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
GHGCGHCRGSGYRGRRAVAEVLLLDDEIRELILTRAPIRSLKEAARRRGFRSLREAVIDM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGDTTLQELNRVTLLG
HCCCCHHHHHCCHHCCCC
>Mature Secondary Structure
MNAPVDLHPRSAPAFSADELARASAAGGRLLDGLATVEADPAERLRRLAATCGLNPIHHA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
RLMSLTPDFGAVRFETALQRECVALRDEDGGLVVVIADPFDNDLLDGLAARLGEGFTSAV
HHEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHC
GDREDIAGYLARHEDEVRRAAGLQLGGDDAQQDDGAEDLSLRRISSDASPVVKLVNSTLY
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
DALKQGASDIHLEAVPGGLVIKYRVDGVLARVGGANGAELAEQAISRIKVMAELDIAERR
HHHHCCCCCEEEEECCCCEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPQDGRFKAQAGGREIDFRVSIMPSIHGEDAVLRVLDKAHLTEEMRGLTLESLGFAADTR
CCCCCCEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHH
AVLRRLAGEPYGMLLVTGPTGSGKTTTLYATLGETNHGLDKIITIEDPVEYQLPGVLQIP
HHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEC
VNEKKGLTFARGLRSILRHDPDKIMVGEIRDGETAEIAIQAALTGHLVFTTVHANNVFDV
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEECEEEEEEEECCCHHHH
IGRFMHMGIDPYNLVAALNGVVAQRLVRLNCPSCSEDIAPPASEVEAARLGDASVFRFRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
GHGCGHCRGSGYRGRRAVAEVLLLDDEIRELILTRAPIRSLKEAARRRGFRSLREAVIDM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGDTTLQELNRVTLLG
HCCCCHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]