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The map label for this gene is gspF2 [H]

Identifier: 119898387

GI number: 119898387

Start: 2301369

End: 2302541

Strand: Reverse

Name: gspF2 [H]

Synonym: azo2096

Alternate gene names: 119898387

Gene position: 2302541-2301369 (Counterclockwise)

Preceding gene: 119898388

Following gene: 119898386

Centisome position: 52.62

GC content: 70.42

Gene sequence:

>1173_bases
GTGGACGCCGCCAATACGGTTGTTGCACTCGACCTCGAAGCGGGCTCCGACGACGAGCTGCGTGCGATCGCGCGCAGCCG
CGGGTTGCAGTTGCTGAGCGCGCGGCAAGCCGCCCCGGGTGCCGGCACCGGATGGCTGCGTGCCCGCCAGGCATTTCCGC
TGCTGCTCTTCAACCAGGAGCTGCTGGCGCTGCTGCGCGCCGGCATTCCGCTTGCCGAAGCGGTAGCGGCGCTCGCGGAG
AAGGAGGGGCGCCCCGCGGTTGCCGTGGTGCTGCAGGGCCTGCTGCGCGCACTGCGCGAAGGGCGCAGCCTGTCCGCCGC
GCTCGAAGCCGTGCCCGCGGTGTTTCCACCGCTGTATGTGGCGATGGTCCGCGCCGCGGAGCGCACCAGCGACCTCGACC
GCGCCCTGGAGCGCTATGTCGCCTACCAGCAGCAGGTCGGCGCGCTGCGCGGCAAGCTGGTCAGCGCCGCCATCTACCCG
GCCTTGCTGGTCGGCGTGGGCGGGCTGGTCGTGCTGTTCCTGCTCGGCTACGTGGTGCCGCGCTTCTCGCATATCTACGA
GGACGTCGGCAACGATCTGCCCTTCATGTCGCGTGTGCTGCTCGTCTGGGGGCAATGGATCGAGGCCAACGCCGCGACCG
GCGCGCTGGCGCTGCTGGCGGTGATCGCCGGCATCGTGGTGCTGTCGCGCCACCCCGCATTCCGCGCCTGGCTGGGGCGC
CTGGGCTGGCGCCTGCCGCTGCTGGGCGAACGGCTGCGCGTATTCCAGCTGGCGCGCTTCTATCGCACGCTCGGCATGCT
GCTCTCCGGTGGCATTCCCGTGGTGCCGGCCCTCGGCATGGTCGCCGGATTGCTGACCCATGGCCTGCGCGCGAGCCTTG
ATGGCGCCATCGACCGTGTGCGGGAAGGGCGCGGATTTGCGTGGACGCTGGCCTCCGCCGGCCTGACCACGCCGGTCGCC
CTGCGCATGCTCGAAGTCGGCGAGCGGGCCGGCAATCTGGGCGAGATGCTGACCCGCGCGGCGGAGTTTCACGAAGAGGA
CACCGCGCGCCAGGTGGAATGGCTGACGCGCCTTTTCGGCCCCTTGCTGATGCTCTTCATCGGCTGCGCGATCGGCCTGA
TCGTGGTGCTGATGTACCTGCCGATCTTTCAGCTCGCGGAGACGCTGGGATGA

Upstream 100 bases:

>100_bases
AGTACGACCTGTTCTCCTACGGCACCGACGGCCAGCCGGGCGGTGACGGCGACGCCGCCGACGTGACCAACTGGTGACCA
TGCGCTACCGGATACGGGCG

Downstream 100 bases:

>100_bases
ACGCGCCGGTCGACCTTCATCCGCGGTCGGCGCCGGCGTTTTCCGCCGACGAGCTGGCGCGCGCCTCCGCCGCAGGAGGG
CGCCTGCTCGACGGCCTCGC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDAANTVVALDLEAGSDDELRAIARSRGLQLLSARQAAPGAGTGWLRARQAFPLLLFNQELLALLRAGIPLAEAVAALAE
KEGRPAVAVVLQGLLRALREGRSLSAALEAVPAVFPPLYVAMVRAAERTSDLDRALERYVAYQQQVGALRGKLVSAAIYP
ALLVGVGGLVVLFLLGYVVPRFSHIYEDVGNDLPFMSRVLLVWGQWIEANAATGALALLAVIAGIVVLSRHPAFRAWLGR
LGWRLPLLGERLRVFQLARFYRTLGMLLSGGIPVVPALGMVAGLLTHGLRASLDGAIDRVREGRGFAWTLASAGLTTPVA
LRMLEVGERAGNLGEMLTRAAEFHEEDTARQVEWLTRLFGPLLMLFIGCAIGLIVVLMYLPIFQLAETLG

Sequences:

>Translated_390_residues
MDAANTVVALDLEAGSDDELRAIARSRGLQLLSARQAAPGAGTGWLRARQAFPLLLFNQELLALLRAGIPLAEAVAALAE
KEGRPAVAVVLQGLLRALREGRSLSAALEAVPAVFPPLYVAMVRAAERTSDLDRALERYVAYQQQVGALRGKLVSAAIYP
ALLVGVGGLVVLFLLGYVVPRFSHIYEDVGNDLPFMSRVLLVWGQWIEANAATGALALLAVIAGIVVLSRHPAFRAWLGR
LGWRLPLLGERLRVFQLARFYRTLGMLLSGGIPVVPALGMVAGLLTHGLRASLDGAIDRVREGRGFAWTLASAGLTTPVA
LRMLEVGERAGNLGEMLTRAAEFHEEDTARQVEWLTRLFGPLLMLFIGCAIGLIVVLMYLPIFQLAETLG
>Mature_390_residues
MDAANTVVALDLEAGSDDELRAIARSRGLQLLSARQAAPGAGTGWLRARQAFPLLLFNQELLALLRAGIPLAEAVAALAE
KEGRPAVAVVLQGLLRALREGRSLSAALEAVPAVFPPLYVAMVRAAERTSDLDRALERYVAYQQQVGALRGKLVSAAIYP
ALLVGVGGLVVLFLLGYVVPRFSHIYEDVGNDLPFMSRVLLVWGQWIEANAATGALALLAVIAGIVVLSRHPAFRAWLGR
LGWRLPLLGERLRVFQLARFYRTLGMLLSGGIPVVPALGMVAGLLTHGLRASLDGAIDRVREGRGFAWTLASAGLTTPVA
LRMLEVGERAGNLGEMLTRAAEFHEEDTARQVEWLTRLFGPLLMLFIGCAIGLIVVLMYLPIFQLAETLG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=390, Percent_Identity=28.7179487179487, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786295, Length=339, Percent_Identity=29.4985250737463, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 41949; Mature: 41949

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAANTVVALDLEAGSDDELRAIARSRGLQLLSARQAAPGAGTGWLRARQAFPLLLFNQE
CCCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHH
LLALLRAGIPLAEAVAALAEKEGRPAVAVVLQGLLRALREGRSLSAALEAVPAVFPPLYV
HHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AMVRAAERTSDLDRALERYVAYQQQVGALRGKLVSAAIYPALLVGVGGLVVLFLLGYVVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFSHIYEDVGNDLPFMSRVLLVWGQWIEANAATGALALLAVIAGIVVLSRHPAFRAWLGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGWRLPLLGERLRVFQLARFYRTLGMLLSGGIPVVPALGMVAGLLTHGLRASLDGAIDRV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REGRGFAWTLASAGLTTPVALRMLEVGERAGNLGEMLTRAAEFHEEDTARQVEWLTRLFG
HCCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLMLFIGCAIGLIVVLMYLPIFQLAETLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAANTVVALDLEAGSDDELRAIARSRGLQLLSARQAAPGAGTGWLRARQAFPLLLFNQE
CCCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHH
LLALLRAGIPLAEAVAALAEKEGRPAVAVVLQGLLRALREGRSLSAALEAVPAVFPPLYV
HHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AMVRAAERTSDLDRALERYVAYQQQVGALRGKLVSAAIYPALLVGVGGLVVLFLLGYVVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFSHIYEDVGNDLPFMSRVLLVWGQWIEANAATGALALLAVIAGIVVLSRHPAFRAWLGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGWRLPLLGERLRVFQLARFYRTLGMLLSGGIPVVPALGMVAGLLTHGLRASLDGAIDRV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REGRGFAWTLASAGLTTPVALRMLEVGERAGNLGEMLTRAAEFHEEDTARQVEWLTRLFG
HCCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLMLFIGCAIGLIVVLMYLPIFQLAETLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]