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The map label for this gene is tig

Identifier: 119898363

GI number: 119898363

Start: 2274050

End: 2275363

Strand: Reverse

Name: tig

Synonym: azo2072

Alternate gene names: 119898363

Gene position: 2275363-2274050 (Counterclockwise)

Preceding gene: 119898371

Following gene: 119898362

Centisome position: 52.0

GC content: 64.46

Gene sequence:

>1314_bases
ATGCAGACCACCCAGGAAATCCAGACCTCGCAGAACCCGCTCGAACGTCGTATCGACATGTCGCTGGCGGTGGCCGACAT
CGACAAGGCGGTGGAAGCCCGCCTGAAGCAGATGGCGCGGTCCGTGAAGATGCCGGGCTTCCGTCCGGGCAAGGTGCCCT
TGAAGATCGTCGAGCAGACCTACGGCCCGCAGGCGCGGTCGGAAGCGATCGGCGCCGCGGTGGAGCGCGCGTTCGGCGAG
AAAGTTCGGGAACAGAACTTGCGTGTTGCCGGCTATCCGCGTATCGAGCCTAAGGAGGCGGGTAGCGAGGGTGCGCTGGA
GTTCAGCGCGGTCTTCGAGGTCTATCCGGAAGTGGTAGTGGGCGATCTGTCTGCACAATCCATCGAACAGCCGACCCTCG
TCGTGGGCGACGCCGAGGTCGACAAGACCATCGACGTGCTGCGCAAGCAGCGCACGACGTTCGAGGCCGCCGCACGCCCC
ACCCAGGACGGCGATCGCGTCACGATCGACTTCGCCGGCCGCAAGGATGGCGAACTGTTCGAAGGCGGTCAGGCGACCGA
CTTCCCGTTCGTGATCGGCGCGGGTTCGATGCTCAAGGATTTCGAGACTGCTGTCGTTGGTCTCGGCGTTGGCGAAACCA
AGACCTTCGACATGACCTTCCCGGAGGACTACCACGCCAAGCATCTCGCCGGCCAGCCGGTGCAGTTCGAGGTCACGCTG
AAGTCGGTCGAAGCGCCCAAGCTGCCCGAAGTCGACAGCGACTTCGCCAAGGCGCTGGGTGTGGCCGACGGCAACGTCGA
CAAGCTGCGTGAAGAGGTCAAGGGCAACCTGGAGCGCGAAGTGAAGCGCCGCATCCAGGCCAAGGTGAAGGAGCAGGCGA
TGGACGCGCTGCTCGCGGTGACGCCGATCGAAGTGCCGAAGGCGCTGGTCGAGTCGGAGGCCACTCAACTGGCCGAGAAC
GCCAAACGTGATCTGGCCGCGCGCGGCATGAACACCAAGGACATCCCGGTGCAGCCGGCGTGGTTCGGCGACCAGGCGAT
GCGCCGCGTGAAGCTGGGCCTGATCATGGCCGAGGTGGTGAAGGGTAACGAACTCCACGCCAAGCCCGAGCAGATCCGTG
GCCTGGTCGAAGAGATGGCGCAGAGCTACGAGGATCCCTCGGAACTCGTGCGCTGGTACTACGCTCAGCCTGACCGGCTG
GCGCAGGCCGAAGCGGTGGTGATCGAGGACAACGTCGTCGCCTGGGTGCTGTCCAAGGCTCAGACCAGCGAGAAGTCGAT
CGCCTTCGATGATTTGATGGGCAATGCGGCCTAA

Upstream 100 bases:

>100_bases
GCGCGCCGATCGGCTTGGCGGCACGCTCGGTCGTGGTTTTTCGCAGCTGCCATAAGCGCCGGTTTCGGCAGCAATTATTT
GACTTCTCAGGATCGTTTTA

Downstream 100 bases:

>100_bases
TCCAAGTAAGGACTGACCATGAACGCAGGAACACCACAGCACAGCAGCGCCTGGGACCCGGTTGGACTGGGTCTGATCCC
GATGGTCGTGGAACAGAGCG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MQTTQEIQTSQNPLERRIDMSLAVADIDKAVEARLKQMARSVKMPGFRPGKVPLKIVEQTYGPQARSEAIGAAVERAFGE
KVREQNLRVAGYPRIEPKEAGSEGALEFSAVFEVYPEVVVGDLSAQSIEQPTLVVGDAEVDKTIDVLRKQRTTFEAAARP
TQDGDRVTIDFAGRKDGELFEGGQATDFPFVIGAGSMLKDFETAVVGLGVGETKTFDMTFPEDYHAKHLAGQPVQFEVTL
KSVEAPKLPEVDSDFAKALGVADGNVDKLREEVKGNLEREVKRRIQAKVKEQAMDALLAVTPIEVPKALVESEATQLAEN
AKRDLAARGMNTKDIPVQPAWFGDQAMRRVKLGLIMAEVVKGNELHAKPEQIRGLVEEMAQSYEDPSELVRWYYAQPDRL
AQAEAVVIEDNVVAWVLSKAQTSEKSIAFDDLMGNAA

Sequences:

>Translated_437_residues
MQTTQEIQTSQNPLERRIDMSLAVADIDKAVEARLKQMARSVKMPGFRPGKVPLKIVEQTYGPQARSEAIGAAVERAFGE
KVREQNLRVAGYPRIEPKEAGSEGALEFSAVFEVYPEVVVGDLSAQSIEQPTLVVGDAEVDKTIDVLRKQRTTFEAAARP
TQDGDRVTIDFAGRKDGELFEGGQATDFPFVIGAGSMLKDFETAVVGLGVGETKTFDMTFPEDYHAKHLAGQPVQFEVTL
KSVEAPKLPEVDSDFAKALGVADGNVDKLREEVKGNLEREVKRRIQAKVKEQAMDALLAVTPIEVPKALVESEATQLAEN
AKRDLAARGMNTKDIPVQPAWFGDQAMRRVKLGLIMAEVVKGNELHAKPEQIRGLVEEMAQSYEDPSELVRWYYAQPDRL
AQAEAVVIEDNVVAWVLSKAQTSEKSIAFDDLMGNAA
>Mature_437_residues
MQTTQEIQTSQNPLERRIDMSLAVADIDKAVEARLKQMARSVKMPGFRPGKVPLKIVEQTYGPQARSEAIGAAVERAFGE
KVREQNLRVAGYPRIEPKEAGSEGALEFSAVFEVYPEVVVGDLSAQSIEQPTLVVGDAEVDKTIDVLRKQRTTFEAAARP
TQDGDRVTIDFAGRKDGELFEGGQATDFPFVIGAGSMLKDFETAVVGLGVGETKTFDMTFPEDYHAKHLAGQPVQFEVTL
KSVEAPKLPEVDSDFAKALGVADGNVDKLREEVKGNLEREVKRRIQAKVKEQAMDALLAVTPIEVPKALVESEATQLAEN
AKRDLAARGMNTKDIPVQPAWFGDQAMRRVKLGLIMAEVVKGNELHAKPEQIRGLVEEMAQSYEDPSELVRWYYAQPDRL
AQAEAVVIEDNVVAWVLSKAQTSEKSIAFDDLMGNAA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=40.045766590389, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_AZOSB (A1K784)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933576.1
- ProteinModelPortal:   A1K784
- SMR:   A1K784
- STRING:   A1K784
- GeneID:   4608489
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2072
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   A1K784
- ProtClustDB:   PRK01490
- BioCyc:   ASP62928:AZO2072-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47982; Mature: 47982

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTQEIQTSQNPLERRIDMSLAVADIDKAVEARLKQMARSVKMPGFRPGKVPLKIVEQT
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YGPQARSEAIGAAVERAFGEKVREQNLRVAGYPRIEPKEAGSEGALEFSAVFEVYPEVVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHE
GDLSAQSIEQPTLVVGDAEVDKTIDVLRKQRTTFEAAARPTQDGDRVTIDFAGRKDGELF
ECCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
EGGQATDFPFVIGAGSMLKDFETAVVGLGVGETKTFDMTFPEDYHAKHLAGQPVQFEVTL
CCCCCCCCCEEEECCHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHCCCCEEEEEEE
KSVEAPKLPEVDSDFAKALGVADGNVDKLREEVKGNLEREVKRRIQAKVKEQAMDALLAV
ECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TPIEVPKALVESEATQLAENAKRDLAARGMNTKDIPVQPAWFGDQAMRRVKLGLIMAEVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KGNELHAKPEQIRGLVEEMAQSYEDPSELVRWYYAQPDRLAQAEAVVIEDNVVAWVLSKA
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCEEEEECCHHEEHHHHH
QTSEKSIAFDDLMGNAA
HCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MQTTQEIQTSQNPLERRIDMSLAVADIDKAVEARLKQMARSVKMPGFRPGKVPLKIVEQT
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YGPQARSEAIGAAVERAFGEKVREQNLRVAGYPRIEPKEAGSEGALEFSAVFEVYPEVVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHE
GDLSAQSIEQPTLVVGDAEVDKTIDVLRKQRTTFEAAARPTQDGDRVTIDFAGRKDGELF
ECCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
EGGQATDFPFVIGAGSMLKDFETAVVGLGVGETKTFDMTFPEDYHAKHLAGQPVQFEVTL
CCCCCCCCCEEEECCHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHCCCCEEEEEEE
KSVEAPKLPEVDSDFAKALGVADGNVDKLREEVKGNLEREVKRRIQAKVKEQAMDALLAV
ECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TPIEVPKALVESEATQLAENAKRDLAARGMNTKDIPVQPAWFGDQAMRRVKLGLIMAEVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KGNELHAKPEQIRGLVEEMAQSYEDPSELVRWYYAQPDRLAQAEAVVIEDNVVAWVLSKA
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCEEEEECCHHEEHHHHH
QTSEKSIAFDDLMGNAA
HCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA