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The map label for this gene is mhpE [H]

Identifier: 119898263

GI number: 119898263

Start: 2168585

End: 2169613

Strand: Reverse

Name: mhpE [H]

Synonym: azo1972

Alternate gene names: 119898263

Gene position: 2169613-2168585 (Counterclockwise)

Preceding gene: 119898264

Following gene: 119898262

Centisome position: 49.58

GC content: 65.89

Gene sequence:

>1029_bases
ATGGACCTTCGCGGCACACGCATCACCGTTCACGACATGACCCTGCGCGACGGCATGCACCCCAAGCGCCACCTGATGAC
GCTCGAGCAGATGAAGACCATCGCCCAGGGGCTCGATCAAGCGGGCATCCCGCTGATCGAAGTCACCCACGGCGACGGTC
TCGGCGGCAGCTCGGTGAACTACGGCTTTCCGGCCCACACCGACGAGGAATACCTCGGCGCGGTGATCCCGCTGATGAAG
CAGGCCAAGGTCTCGGCACTGCTGCTGCCGGGCATCGGCACCGTCGATCACCTGAAGATGGCGCACGAACTCGGGGTGAA
CACCATCCGTGTGGCCACCCACTGCACCGAAGCCGACGTCTCCGAACAGCACATCAGTTACGCGAGAAAGCTCGGCATGG
ACACCGTGGGCTTTCTGATGATGGCCCACATGAACAGCCCCGAAGGGCTGGTGCAGCAGGCGAAGCTGATGGAGAGCTAC
GGCGCCAACTGCATCTACGTGACCGATTCGGCCGGGCATCTGCTGCCGGATACGGTCAAGGCGCGCATCAGCGCCGTGCG
GGATGCGCTGAAACCCGAAACCGAACTCGGCTTCCACGGCCACCACAACCTCGCGATGGGCGTCGCCAACTCCATCGCCG
CGATCGAAGCCGGGGCGACCCGCATCGACGCCGCGGCCGCAGGGCTGGGCGCCGGCGCCGGCAACACGCCGATGGAAGTG
CTGATCGCGGTGTGCGACCTGATGGGGATCGAGACCGGCGTCGATGTGTTCAAGATCCAGGACGTCGCCGAAGACCTCGT
GGTGCCCATCATGGACTTCCCGATCCGCATCGACCGCGATGCGCTGACGCTGGGCTATGCCGGCGTGTACGGCTCCTTCC
TGCTCTTTGCCAAGCGCGCCGAGAAGAAGTACGGCGTGCCGGCGCGCGAGATCCTGGTCGAGATGGGCCGGCGCGGCATG
GTCGGCGGCCAGGAGGACATGATCGAGGACACCGCGATCACCCTGGCGAAGGCGAGCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGGTGCCCGCACCGCCGAGATGTTCGCCGAGGAAATCCTCGCCGGCCGCCTCACCCTCGAATCCAACCGCGCCGTGCTG
GCTTGAATCAGGAGAAAACC

Downstream 100 bases:

>100_bases
ACGCGACCACACGGCAGGCCGCGCCGTCGGCGCGGCCCACATCCAAGGAGAGCGAGACATGAAAACCATGAAGGCGGCCC
GCCTGCACGAGATCGGCGGC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 3; 4-hydroxy-2-keto-pentanoic acid aldolase 3; 4-hydroxy-2-oxopentanoate aldolase 3 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKASAAA

Sequences:

>Translated_342_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKASAAA
>Mature_342_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKASAAA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=333, Percent_Identity=57.3573573573574, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1786261, Length=295, Percent_Identity=25.4237288135593, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36454; Mature: 36454

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
SEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESYGANCIYVTDSAGHLLPDTVK
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
ARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHEECHHHHHHHHHHHHHHH
EKKYGVPAREILVEMGRRGMVGGQEDMIEDTAITLAKASAAA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
SEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESYGANCIYVTDSAGHLLPDTVK
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
ARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHEECHHHHHHHHHHHHHHH
EKKYGVPAREILVEMGRRGMVGGQEDMIEDTAITLAKASAAA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA