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The map label for this gene is thcR [H]

Identifier: 119898236

GI number: 119898236

Start: 2135167

End: 2136183

Strand: Reverse

Name: thcR [H]

Synonym: azo1945

Alternate gene names: 119898236

Gene position: 2136183-2135167 (Counterclockwise)

Preceding gene: 119898240

Following gene: 119898235

Centisome position: 48.82

GC content: 67.35

Gene sequence:

>1017_bases
ATGGAACTGCAAGGCCACTTTGCGCCCGCGGGCGCAAGACCCCTGCTTGCCAACTTTCCGCTGTTCCGCACCTCGGACCT
CGACGAAGCGCGCGACAAGGTGTCGCGGGTGTTCTGCGACCATCGCCTGCGGGTGGTGGACCGCAGTCAGCGCATCGGCG
CCGAGATGTATCTGCGGCCGATACGCGGCATCGGCGTGGGGCGCATGCGTTACGGCGCCAGCGTGGCGATCGACCCCGGC
CAGCTGGGCGACTTCACGCTGATCCAGATGCCGCTGCGCGGATCGGAAGTGGTGGAGCACGCGGGGCACACGGTCAACTC
CAATACCGACGTGGCCTCGGTGGTCAGCCCCACGCAGGCGGTCAGCATTCGCCACGGGGCCGACACCGACAAACTCTTCA
TCCGCATCGACCGCGAAACGCTGGAACGTCACTGCCGCCAGCACCTGGGCGGCACGCTGCGCGAGCCGGTGGAGTTCCTG
CCGGAGATGGCCCTGGGCAGCAGCCGCTGCCAATCCTGGGTGCGGCTGATGAAGTGGCTGTGCGAGGAAATCGGCCACGA
GAACCCGCAGGGCGAAGCGGTGCTCGATTCGCCGCTGTTCTCCGCGCAGGTGGAACAGATGGTGATCACCACGCTCCTGA
TGTGCCAGCCGCACAACTACTCCGAACGCCTCGCGCAGGATGGCCCGTCGATCGCGCCGCACTTCATCCGCCGCACCGAG
CGCTACATCGAGGACAAGGCCCACGAGCCGCTCACCATCGGCGAACTCGCCGAACACGCGGGCGTCAGCACCCGCTCGCT
GTTCGCCGGCTTCCGCAAGTACCGCGACACCACGCCGATGCAGTACCTGAAGGACGTGCGGATGCAGCGCGTCCATCAGG
AACTGCTGCGCAGCGCCCCCGACACCACCACCGTGACCGACGTGGCGATGCGCTGGGGTTTCTCGCACCTCGGCCACTTC
GCGGTGGACTACAAACGCTGCTTCGGCGAAAGCCCCTCGGCCACGCTCGGCCGCTGA

Upstream 100 bases:

>100_bases
CCTGCGGCTATGGGAAAAGTAATAGACGGGCGGGAACGGCTTCTGTTTTAATTTAGATATAAACAATTCCTGCAATGACA
GGAGCGATGGAGGAGACGCC

Downstream 100 bases:

>100_bases
GTCGCCCAGGCGATTTCTGCAACATCCGGATAGGCGCCAGAGTTTCGGGATAGTTCGCCACGTACCGCGTCACTAGATTG
GGCACACCGGCCGCCAAGAG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MELQGHFAPAGARPLLANFPLFRTSDLDEARDKVSRVFCDHRLRVVDRSQRIGAEMYLRPIRGIGVGRMRYGASVAIDPG
QLGDFTLIQMPLRGSEVVEHAGHTVNSNTDVASVVSPTQAVSIRHGADTDKLFIRIDRETLERHCRQHLGGTLREPVEFL
PEMALGSSRCQSWVRLMKWLCEEIGHENPQGEAVLDSPLFSAQVEQMVITTLLMCQPHNYSERLAQDGPSIAPHFIRRTE
RYIEDKAHEPLTIGELAEHAGVSTRSLFAGFRKYRDTTPMQYLKDVRMQRVHQELLRSAPDTTTVTDVAMRWGFSHLGHF
AVDYKRCFGESPSATLGR

Sequences:

>Translated_338_residues
MELQGHFAPAGARPLLANFPLFRTSDLDEARDKVSRVFCDHRLRVVDRSQRIGAEMYLRPIRGIGVGRMRYGASVAIDPG
QLGDFTLIQMPLRGSEVVEHAGHTVNSNTDVASVVSPTQAVSIRHGADTDKLFIRIDRETLERHCRQHLGGTLREPVEFL
PEMALGSSRCQSWVRLMKWLCEEIGHENPQGEAVLDSPLFSAQVEQMVITTLLMCQPHNYSERLAQDGPSIAPHFIRRTE
RYIEDKAHEPLTIGELAEHAGVSTRSLFAGFRKYRDTTPMQYLKDVRMQRVHQELLRSAPDTTTVTDVAMRWGFSHLGHF
AVDYKRCFGESPSATLGR
>Mature_338_residues
MELQGHFAPAGARPLLANFPLFRTSDLDEARDKVSRVFCDHRLRVVDRSQRIGAEMYLRPIRGIGVGRMRYGASVAIDPG
QLGDFTLIQMPLRGSEVVEHAGHTVNSNTDVASVVSPTQAVSIRHGADTDKLFIRIDRETLERHCRQHLGGTLREPVEFL
PEMALGSSRCQSWVRLMKWLCEEIGHENPQGEAVLDSPLFSAQVEQMVITTLLMCQPHNYSERLAQDGPSIAPHFIRRTE
RYIEDKAHEPLTIGELAEHAGVSTRSLFAGFRKYRDTTPMQYLKDVRMQRVHQELLRSAPDTTTVTDVAMRWGFSHLGHF
AVDYKRCFGESPSATLGR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=102, Percent_Identity=38.2352941176471, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38116; Mature: 38116

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQGHFAPAGARPLLANFPLFRTSDLDEARDKVSRVFCDHRLRVVDRSQRIGAEMYLRP
CCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
IRGIGVGRMRYGASVAIDPGQLGDFTLIQMPLRGSEVVEHAGHTVNSNTDVASVVSPTQA
HHCCCCCHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHCCHHH
VSIRHGADTDKLFIRIDRETLERHCRQHLGGTLREPVEFLPEMALGSSRCQSWVRLMKWL
EEECCCCCCCEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CEEIGHENPQGEAVLDSPLFSAQVEQMVITTLLMCQPHNYSERLAQDGPSIAPHFIRRTE
HHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHH
RYIEDKAHEPLTIGELAEHAGVSTRSLFAGFRKYRDTTPMQYLKDVRMQRVHQELLRSAP
HHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
DTTTVTDVAMRWGFSHLGHFAVDYKRCFGESPSATLGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MELQGHFAPAGARPLLANFPLFRTSDLDEARDKVSRVFCDHRLRVVDRSQRIGAEMYLRP
CCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
IRGIGVGRMRYGASVAIDPGQLGDFTLIQMPLRGSEVVEHAGHTVNSNTDVASVVSPTQA
HHCCCCCHHHCCCEEEECCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHCCHHH
VSIRHGADTDKLFIRIDRETLERHCRQHLGGTLREPVEFLPEMALGSSRCQSWVRLMKWL
EEECCCCCCCEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CEEIGHENPQGEAVLDSPLFSAQVEQMVITTLLMCQPHNYSERLAQDGPSIAPHFIRRTE
HHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHH
RYIEDKAHEPLTIGELAEHAGVSTRSLFAGFRKYRDTTPMQYLKDVRMQRVHQELLRSAP
HHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
DTTTVTDVAMRWGFSHLGHFAVDYKRCFGESPSATLGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]