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The map label for this gene is acnB [H]

Identifier: 119897825

GI number: 119897825

Start: 1678824

End: 1681421

Strand: Direct

Name: acnB [H]

Synonym: azo1534

Alternate gene names: 119897825

Gene position: 1678824-1681421 (Clockwise)

Preceding gene: 119897822

Following gene: 119897826

Centisome position: 38.36

GC content: 66.01

Gene sequence:

>2598_bases
GTGCTTGAAGCCTACCGTGCCCATGTCGCCGAGCGTGCCGCCCTCGGCATCCCGCCGCTGCCCCTGACCAAGCAGCAGAC
CGCCGAGCTGGTCGAACTGCTGAAGAATCCCCCCGCCGGCGAGGAAGCCTTCCTTGTCGAGCTGCTGACCCACCGTGTGC
CGGCCGGCGTGGACGACGCCGCCAAGGTGAAGGCCGAGTTCCTGGCCAAGGTCGCCAAGGGCGAAGAATCCTGTGGCCTC
GTGTCGCGCGCCAAGGCCACCGAACTGCTGGGCACCATGCTCGGCGGTTTCAACATCAAGCCGCTGATCGACCTGCTGGC
CGATGCCGAAGTGGGCGCTGTCGCCGCCGCCGGCCTCAAGACCACGCTGCTGATGTTCGACTACTTCCACGACGTCAAGG
AACTGGCCGAAAAGGGCAACGCCAACGCCAAGGCCGTGATGCAGTCGTGGGCCGACGCCGAGTGGTTCACCAGCCGCCCG
GAAGTGCCGGCCTCGCAGAAGCTGACCGTGTTCAAGGTCACCGGCGAAACCAACACCGACGACCTTTCCCCGGCGCCGGA
TGCCTGGTCCCGCCCGGACATCCCGCTGCACGCGCTGGCGATGCTGAAGAACCCGCGTCCGGGCATCACCCCGGAAGAGC
CGGGCGTGCGTGGCCCGGTCAAGTTCCTCGAAGAACTCAAGGCCAAGGGCAACCTCGTCGCCTACGTGGGCGACGTGGTC
GGCACCGGCTCCTCGCGCAAGTCCGCCACCAACTCGGTGCTGTGGTTCACCGGCGAAGACATCCCCTTCGTGCCGAACAA
GCGTTTCGGCGGGGTGTGCCTGGGTTCGAAGATCGCCCCGATCTTCTTCAACACCATGGAAGACGCGGGTGCGCTGCCGA
TCGAAATCGACGCCGGCCAGATGGACATGGGCGACGAGATCGAGCTGAAGGTCGACCAGGCCACCGGCAAGGTCACCGCG
CTCAAGAACGGCGCCGTGATCGCTGAAAGCCAGCTCAAGACCCTGGTGATCCTCGACGAAGTGCGCGCCGGCGGCCGCAT
TCCGCTGATCATCGGCCGCGGCCTCACCGCCAAGGCGCGCGAAGCGCTGGGCCTGCCGCCTTCCACGCTGTTCCGCCTGC
CGCAAGCGCCGGTCGATACCGGCAAGGGCTTCTCGCTGGCGCAGAAGATGGTCGGCCGCGCCTGCGGTCTGCCGGAAGGC
CAGGGCATCCGCCCGGGCACCTACTGCGAACCGAAGATGACCACCGTCGGCTCGCAGGACACCACCGGCCCGATGACCCG
CGACGAACTGAAGGACCTCGCCTGCCTCGGCTTCTCCGCCGACATGGTGATGCAGTCCTTCTGCCACACCGCGGCCTACC
CGAAGCTGGTTGACGTGAAGATGCACCGCGAGCTGCCGAGCTTCATCAGCACCCGCGGCGGCGTGGCCCTGCGTCCGGGC
GACGGCGTGATCCACTCCTGGCTCAACCGCCTGCTGCTGCCCGATACCGTCGGCACCGGCGGCGACAGCCACACCCGTTT
CCCGATCGGCATCTCCTTCCCGGCCGGTTCCGGCCTGGTCGCCTTTGCCGCCGCGACCGGCGTGATGCCGCTCGACATGC
CGGAATCGGTGCTGGTGCGCTTCAAGGGCACCATGCAGCCGGGCGTCACCCTGCGTGACCTGGTCAACGCGATCCCGCTG
TACGCGATCAAGGCCGGCCTGCTGACCGTCGAGAAGAAGGGCAAGAAGAACATCTTCTCCGGCCGCATCCTCGAAATCGA
AGGTCTGCCGGATCTGAAGGTCGAACAGGCCTTCGAACTGACCGACGCCTCGGCCGAACGCTCCGCCGCCGGCTGCACGG
TCCACCTCAACAAGGCGCCGATCGTCGAGTACATGAACTCGAACATCACGCTGATGAAGTGGATGATCGCCAACGGCTAC
CAGGATGCCCGCACCCTGCGCCGCCGCATCAAGGCGATGGAAGACTGGATCGCCAACGGCGAACTGCTGTCGGGCGATGC
CGACGCCGAGTACGCCGCGGTCATCGAGATCGATCTGGCCGACATCAAGGAGCCGATCGTCGCGTGCCCGAACGACCCGG
ACGACGTCAAGCTGCTGTCCGAAGTCGCCGGCGACAAGATCGACGAAGTCTTCATCGGCTCGTGCATGACCAACATCGGC
CACTTCCGCGCTGCCGGCAAGGTGCTGGACGGCAAGTCCGACATCCCGACCCGCCTGTGGATCGCCCCGCCCACCAAGAT
GGACGCGATGATCCTCAACGAGGAAGGCTACTACGGCGTGCTCGGCAAGTCCGGCGCCCGCATGGAAATGCCGGGCTGCT
CGCTGTGCATGGGCAACCAGGCGCAGATCCGCAAGGGCAGCACCGCGATGTCGACCTCGACGCGCAACTTCCCCAACCGT
CTCGGCATCGACACCCGCGTGTATCTGGGTTCGGCCGAACTGTCGGCGGTGTGCGCGCTGCTGGGCAAGATCCCGACCCC
GGCCGAGTACATGGCGCAGGTGGAAGTGGTCAACAAGAAGGCCGCCGATATCTACCGCTACATGAACTTCGACCAGATCG
AAGAGTTCAAGAGCGTCGCCGATACCGTCGAAGTTTAA

Upstream 100 bases:

>100_bases
CACCGCAAGTCTTATAAAAGACATATAATGCCGTCCGTCCGTGCGGGCGAACCCCGCGCCGGAGCCCTTGCCACCGTCAC
GAAACTGGAAGGAAGTCGCC

Downstream 100 bases:

>100_bases
GGTTTAGAGGTAGGGAGACCGGAGCAGGGCGCCCAAGAGCGCCCGCCCGCCGATGTAACGCCCCTGTCCGGGTTGCCGGC
AGGGGCGTTTTGTTTTGATT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRAHVAERAALGIPPLPLTKQQTAELVELLKNPPAGEEAFLVELLTHRVPAGVDDAAKVKAEFLAKVAKGEESCGL
VSRAKATELLGTMLGGFNIKPLIDLLADAEVGAVAAAGLKTTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRP
EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEPGVRGPVKFLEELKAKGNLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFFNTMEDAGALPIEIDAGQMDMGDEIELKVDQATGKVTA
LKNGAVIAESQLKTLVILDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPQAPVDTGKGFSLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKLVDVKMHRELPSFISTRGGVALRPG
DGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPL
YAIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKAPIVEYMNSNITLMKWMIANGY
QDARTLRRRIKAMEDWIANGELLSGDADAEYAAVIEIDLADIKEPIVACPNDPDDVKLLSEVAGDKIDEVFIGSCMTNIG
HFRAAGKVLDGKSDIPTRLWIAPPTKMDAMILNEEGYYGVLGKSGARMEMPGCSLCMGNQAQIRKGSTAMSTSTRNFPNR
LGIDTRVYLGSAELSAVCALLGKIPTPAEYMAQVEVVNKKAADIYRYMNFDQIEEFKSVADTVEV

Sequences:

>Translated_865_residues
MLEAYRAHVAERAALGIPPLPLTKQQTAELVELLKNPPAGEEAFLVELLTHRVPAGVDDAAKVKAEFLAKVAKGEESCGL
VSRAKATELLGTMLGGFNIKPLIDLLADAEVGAVAAAGLKTTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRP
EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEPGVRGPVKFLEELKAKGNLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFFNTMEDAGALPIEIDAGQMDMGDEIELKVDQATGKVTA
LKNGAVIAESQLKTLVILDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPQAPVDTGKGFSLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKLVDVKMHRELPSFISTRGGVALRPG
DGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPL
YAIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKAPIVEYMNSNITLMKWMIANGY
QDARTLRRRIKAMEDWIANGELLSGDADAEYAAVIEIDLADIKEPIVACPNDPDDVKLLSEVAGDKIDEVFIGSCMTNIG
HFRAAGKVLDGKSDIPTRLWIAPPTKMDAMILNEEGYYGVLGKSGARMEMPGCSLCMGNQAQIRKGSTAMSTSTRNFPNR
LGIDTRVYLGSAELSAVCALLGKIPTPAEYMAQVEVVNKKAADIYRYMNFDQIEEFKSVADTVEV
>Mature_865_residues
MLEAYRAHVAERAALGIPPLPLTKQQTAELVELLKNPPAGEEAFLVELLTHRVPAGVDDAAKVKAEFLAKVAKGEESCGL
VSRAKATELLGTMLGGFNIKPLIDLLADAEVGAVAAAGLKTTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRP
EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEPGVRGPVKFLEELKAKGNLVAYVGDVV
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFFNTMEDAGALPIEIDAGQMDMGDEIELKVDQATGKVTA
LKNGAVIAESQLKTLVILDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPQAPVDTGKGFSLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKLVDVKMHRELPSFISTRGGVALRPG
DGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPL
YAIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKAPIVEYMNSNITLMKWMIANGY
QDARTLRRRIKAMEDWIANGELLSGDADAEYAAVIEIDLADIKEPIVACPNDPDDVKLLSEVAGDKIDEVFIGSCMTNIG
HFRAAGKVLDGKSDIPTRLWIAPPTKMDAMILNEEGYYGVLGKSGARMEMPGCSLCMGNQAQIRKGSTAMSTSTRNFPNR
LGIDTRVYLGSAELSAVCALLGKIPTPAEYMAQVEVVNKKAADIYRYMNFDQIEEFKSVADTVEV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=864, Percent_Identity=73.6111111111111, Blast_Score=1254, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=380, Percent_Identity=25.2631578947368, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=483, Percent_Identity=24.4306418219462, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92798; Mature: 92798

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRAHVAERAALGIPPLPLTKQQTAELVELLKNPPAGEEAFLVELLTHRVPAGVDDA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKAEFLAKVAKGEESCGLVSRAKATELLGTMLGGFNIKPLIDLLADAEVGAVAAAGLK
HHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
TTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRPEVPASQKLTVFKVTGETNTD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEECCCCCC
DLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEPGVRGPVKFLEELKAKGNLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFFNTMEDAGALPIEIDAGQ
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHEECHHCCCCCEEEECCCC
MDMGDEIELKVDQATGKVTALKNGAVIAESQLKTLVILDEVRAGGRIPLIIGRGLTAKAR
CCCCCCEEEEEECCCCCEEEECCCCEEECCCCCEEEEEEHHCCCCCEEEEEECCCCHHHH
EALGLPPSTLFRLPQAPVDTGKGFSLAQKMVGRACGLPEGQGIRPGTYCEPKMTTVGSQD
HHCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKLVDVKMHRELPSFISTRGGVALRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCEEECCC
DGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGTMQPGVTLRDLVNAIPLYAIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFEL
ECCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHH
TDASAERSAAGCTVHLNKAPIVEYMNSNITLMKWMIANGYQDARTLRRRIKAMEDWIANG
CCCCCCCCCCCEEEEECCCCHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHCCC
ELLSGDADAEYAAVIEIDLADIKEPIVACPNDPDDVKLLSEVAGDKIDEVFIGSCMTNIG
CEECCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
HFRAAGKVLDGKSDIPTRLWIAPPTKMDAMILNEEGYYGVLGKSGARMEMPGCSLCMGNQ
HHHHCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCEEECCCCCEECCCH
AQIRKGSTAMSTSTRNFPNRLGIDTRVYLGSAELSAVCALLGKIPTPAEYMAQVEVVNKK
HHHCCCCCHHCCCCCCCCHHCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AADIYRYMNFDQIEEFKSVADTVEV
HHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEAYRAHVAERAALGIPPLPLTKQQTAELVELLKNPPAGEEAFLVELLTHRVPAGVDDA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKAEFLAKVAKGEESCGLVSRAKATELLGTMLGGFNIKPLIDLLADAEVGAVAAAGLK
HHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
TTLLMFDYFHDVKELAEKGNANAKAVMQSWADAEWFTSRPEVPASQKLTVFKVTGETNTD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEECCCCCC
DLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEPGVRGPVKFLEELKAKGNLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFFNTMEDAGALPIEIDAGQ
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHEECHHCCCCCEEEECCCC
MDMGDEIELKVDQATGKVTALKNGAVIAESQLKTLVILDEVRAGGRIPLIIGRGLTAKAR
CCCCCCEEEEEECCCCCEEEECCCCEEECCCCCEEEEEEHHCCCCCEEEEEECCCCHHHH
EALGLPPSTLFRLPQAPVDTGKGFSLAQKMVGRACGLPEGQGIRPGTYCEPKMTTVGSQD
HHCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKLVDVKMHRELPSFISTRGGVALRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCEEECCC
DGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGTMQPGVTLRDLVNAIPLYAIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFEL
ECCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHH
TDASAERSAAGCTVHLNKAPIVEYMNSNITLMKWMIANGYQDARTLRRRIKAMEDWIANG
CCCCCCCCCCCEEEEECCCCHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHCCC
ELLSGDADAEYAAVIEIDLADIKEPIVACPNDPDDVKLLSEVAGDKIDEVFIGSCMTNIG
CEECCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
HFRAAGKVLDGKSDIPTRLWIAPPTKMDAMILNEEGYYGVLGKSGARMEMPGCSLCMGNQ
HHHHCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCEEECCCCCEECCCH
AQIRKGSTAMSTSTRNFPNRLGIDTRVYLGSAELSAVCALLGKIPTPAEYMAQVEVVNKK
HHHCCCCCHHCCCCCCCCHHCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AADIYRYMNFDQIEEFKSVADTVEV
HHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]