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The map label for this gene is yfgF [H]

Identifier: 119897777

GI number: 119897777

Start: 1622923

End: 1624122

Strand: Reverse

Name: yfgF [H]

Synonym: azo1486

Alternate gene names: 119897777

Gene position: 1624122-1622923 (Counterclockwise)

Preceding gene: 119897778

Following gene: 119897775

Centisome position: 37.11

GC content: 68.25

Gene sequence:

>1200_bases
ATGCGACCCAGTCAGGCGCCCAAGAACGGCCATTCCTTTTCCCATCTGGTGCAGGACGCGCTGGGCGCCCTGCGCCGCCA
TCTCGGCATGGAGGTGGCATTCATCTCCGAATTCCGCCACGGCCGGCGCTTCTTCCGCTACGTGGATTCGGACGCCGCGT
TCTCTCCGATCCAGGTCGGCGGCTCCAACCCGCTGGACGAAAGCTACTGCCTGCGCGTGGTCGATGGCCGCCTGCCCCAG
CTCATCCCCGACGCCCTGCAGAACGCCGAGGCCCTGTCGCTGCCGGTCACGCGGCTGCTGCCGGTGGGCGCGCACATCAG
CGTGCCGATCCAGCTGCTCGATGGCGAGGTGTTCGGCACCTTCTGCTGCTTCAGCCGCACCGCCGACCTGTCGCTCGACG
ATCGCGACCTCGCCGTCGTCCGCCTGTTCGCGGAATTCGTCGGCGGCACGGTCGCCCAGGCCTTCCGCCAGCGGAAAAAC
CTCAAAGCCGCTTACCAGCGCGTGCAGCACGCGATCGCGCAGGAGGATTTTCATATCGCGTATCAGCCGATCATTCACGT
CGGCGAGAGCCGGCTGGTCGGTCACGAGGCCCTCGCCCGCTTCACCGCCGAACCGCTGCGCTCGCCCGACAAGTGGTTCC
ACGAAGCGGCCGAGGTCGGCCTGCAGGAGGAACTGGAACTCGCGGCGATCCGCAAGGCGCTGGAGAACCTGCACCGCTTC
CCGGCTCCCACCTACCTGTCGCTCAACATCTCGCCCGAGACCATCCTGAAGGGCGGGTTGCCCGCGGTGCTGGCCGGATA
CCCGCTAGACCGGCTCGTGCTGGAGCTCACCGAGCACGAGTCGATCGACGACTACGCCGCACTCGTGGCCGCACTCGCGC
CGCTGCGCCAGCGCGGCCTGCGCGTGGCGGTGGACGATGCGGGCGCGGGCTACGCCAGCTTCCGCCACATCCTCAAGCTG
GCGCCCGACATCATCAAGCTCGACGCCAGCCTGGTTGCCACCATAGACAAGGACCAGGGCATGCGCGCGCTGGCAGCCGC
GGTGGTGCGCTTCGCCGAGGAAACCGGCAGCAAGGTGGTGGCCGAAGGCGTGGAGCGCCCCGAAGAACTGCAGGTGCTAC
GCGACCTGAAGGTCAACAAGGCGCAGGGCTACCTGCTCGGCCGCCCACGCCGGGCCGAGGACCTGGAATTCCCGGGTTAG

Upstream 100 bases:

>100_bases
CACCGATCGCACGCCTGCGCCACCCGATCTGGGTATGCGGAGAAGGTTTTGGTAGTACGATCGGCACGAACCGATCCGCC
TTCCTTCGCAGGTGATTCGA

Downstream 100 bases:

>100_bases
CTGACGCGCACGCGCCTAACGCAGACCGATGATCATGCTCAGCATCAGCGCCAGCACCACCATCAGCGCCACCCAGGCGA
TCACGCCCGCCATCAGCACG

Product: putative signaling protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRPSQAPKNGHSFSHLVQDALGALRRHLGMEVAFISEFRHGRRFFRYVDSDAAFSPIQVGGSNPLDESYCLRVVDGRLPQ
LIPDALQNAEALSLPVTRLLPVGAHISVPIQLLDGEVFGTFCCFSRTADLSLDDRDLAVVRLFAEFVGGTVAQAFRQRKN
LKAAYQRVQHAIAQEDFHIAYQPIIHVGESRLVGHEALARFTAEPLRSPDKWFHEAAEVGLQEELELAAIRKALENLHRF
PAPTYLSLNISPETILKGGLPAVLAGYPLDRLVLELTEHESIDDYAALVAALAPLRQRGLRVAVDDAGAGYASFRHILKL
APDIIKLDASLVATIDKDQGMRALAAAVVRFAEETGSKVVAEGVERPEELQVLRDLKVNKAQGYLLGRPRRAEDLEFPG

Sequences:

>Translated_399_residues
MRPSQAPKNGHSFSHLVQDALGALRRHLGMEVAFISEFRHGRRFFRYVDSDAAFSPIQVGGSNPLDESYCLRVVDGRLPQ
LIPDALQNAEALSLPVTRLLPVGAHISVPIQLLDGEVFGTFCCFSRTADLSLDDRDLAVVRLFAEFVGGTVAQAFRQRKN
LKAAYQRVQHAIAQEDFHIAYQPIIHVGESRLVGHEALARFTAEPLRSPDKWFHEAAEVGLQEELELAAIRKALENLHRF
PAPTYLSLNISPETILKGGLPAVLAGYPLDRLVLELTEHESIDDYAALVAALAPLRQRGLRVAVDDAGAGYASFRHILKL
APDIIKLDASLVATIDKDQGMRALAAAVVRFAEETGSKVVAEGVERPEELQVLRDLKVNKAQGYLLGRPRRAEDLEFPG
>Mature_399_residues
MRPSQAPKNGHSFSHLVQDALGALRRHLGMEVAFISEFRHGRRFFRYVDSDAAFSPIQVGGSNPLDESYCLRVVDGRLPQ
LIPDALQNAEALSLPVTRLLPVGAHISVPIQLLDGEVFGTFCCFSRTADLSLDDRDLAVVRLFAEFVGGTVAQAFRQRKN
LKAAYQRVQHAIAQEDFHIAYQPIIHVGESRLVGHEALARFTAEPLRSPDKWFHEAAEVGLQEELELAAIRKALENLHRF
PAPTYLSLNISPETILKGGLPAVLAGYPLDRLVLELTEHESIDDYAALVAALAPLRQRGLRVAVDDAGAGYASFRHILKL
APDIIKLDASLVATIDKDQGMRALAAAVVRFAEETGSKVVAEGVERPEELQVLRDLKVNKAQGYLLGRPRRAEDLEFPG

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=247, Percent_Identity=29.9595141700405, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081743, Length=332, Percent_Identity=27.1084337349398, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790496, Length=244, Percent_Identity=27.8688524590164, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081980, Length=240, Percent_Identity=28.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081921, Length=268, Percent_Identity=28.3582089552239, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788502, Length=244, Percent_Identity=28.2786885245902, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787055, Length=271, Percent_Identity=27.3062730627306, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082096, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI226510982, Length=266, Percent_Identity=30.8270676691729, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081845, Length=228, Percent_Identity=28.5087719298246, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786507, Length=231, Percent_Identity=27.7056277056277, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787541, Length=237, Percent_Identity=29.535864978903, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787410, Length=121, Percent_Identity=28.099173553719, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 43938; Mature: 43938

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSQAPKNGHSFSHLVQDALGALRRHLGMEVAFISEFRHGRRFFRYVDSDAAFSPIQVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
GSNPLDESYCLRVVDGRLPQLIPDALQNAEALSLPVTRLLPVGAHISVPIQLLDGEVFGT
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCCEEEEEEECCHHHHH
FCCFSRTADLSLDDRDLAVVRLFAEFVGGTVAQAFRQRKNLKAAYQRVQHAIAQEDFHIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YQPIIHVGESRLVGHEALARFTAEPLRSPDKWFHEAAEVGLQEELELAAIRKALENLHRF
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
PAPTYLSLNISPETILKGGLPAVLAGYPLDRLVLELTEHESIDDYAALVAALAPLRQRGL
CCCCEEEEECCHHHHHCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
RVAVDDAGAGYASFRHILKLAPDIIKLDASLVATIDKDQGMRALAAAVVRFAEETGSKVV
EEEEECCCCCHHHHHHHHHHCCHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHCCHHH
AEGVERPEELQVLRDLKVNKAQGYLLGRPRRAEDLEFPG
HHHCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MRPSQAPKNGHSFSHLVQDALGALRRHLGMEVAFISEFRHGRRFFRYVDSDAAFSPIQVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
GSNPLDESYCLRVVDGRLPQLIPDALQNAEALSLPVTRLLPVGAHISVPIQLLDGEVFGT
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCCEEEEEEECCHHHHH
FCCFSRTADLSLDDRDLAVVRLFAEFVGGTVAQAFRQRKNLKAAYQRVQHAIAQEDFHIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YQPIIHVGESRLVGHEALARFTAEPLRSPDKWFHEAAEVGLQEELELAAIRKALENLHRF
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
PAPTYLSLNISPETILKGGLPAVLAGYPLDRLVLELTEHESIDDYAALVAALAPLRQRGL
CCCCEEEEECCHHHHHCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
RVAVDDAGAGYASFRHILKLAPDIIKLDASLVATIDKDQGMRALAAAVVRFAEETGSKVV
EEEEECCCCCHHHHHHHHHHCCHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHCCHHH
AEGVERPEELQVLRDLKVNKAQGYLLGRPRRAEDLEFPG
HHHCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]