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The map label for this gene is mnmC

Identifier: 119897773

GI number: 119897773

Start: 1615045

End: 1616952

Strand: Reverse

Name: mnmC

Synonym: azo1482

Alternate gene names: 119897773

Gene position: 1616952-1615045 (Counterclockwise)

Preceding gene: 119897774

Following gene: 119897772

Centisome position: 36.95

GC content: 74.53

Gene sequence:

>1908_bases
ATGCCGATCACACCCGCCAGCCTCAGCTTCGCCGAAGACGGCACGCCCTACTCCACCGCCTACGGCGACGTCTATCACTC
CAGCGACGGCGGCCTCGGCCAGGCCCACCACGTCTTTCTCGCCGGCAACGGCCTGCCCGAACGCTGGCGCGGCCGCGAGC
GCTTCGTCATCGTCGAGACCGGCTTCGGGCTCGGGCTCAACTTCCTCGCCAGCTGGGCGGCGTGGCGTAAGGACCCGCAG
CGCAGCGAACGGCTGCACTTCGTCTCCTGCGAGCTGCATCCCTTCCGCGTCGAAGACCTCGCGCTGCTGCACGCGCGCTG
GCCGGAGTTCGCGCCGCTCGCCGCCGAGCTGCAGGCCAACTGGCCCTGCCTCGCGCCCGGCGTGCATCGGCTTCACCTCG
ATGGCGGCCGCGTCTGCCTCACGCTCTACTTCGGCGATGCGCGCGACGGGCTGGCGCAGCTCGACGCGCGCGCCGACGCC
TTCCTGCTCGACGGCTTCTCGCCGGCGAAGAATCCGGATTTGTGGTCTGCGCGCATCTTCCACCTGCTTGCCCGGCTGGC
CGCGGCGGACGCGACCCTCGCGACCTGGTCGGTTGCCGGCGAGGTGCGCGAAGGGCTGCGACGCGCCGGCTTCGAAGTGG
AGAAGGCACCCGGCTTCGGCGGCAAGCGGCAGATGCTGCGCGGACGTTACCTCGGGCGCGGACACCGCCCGGCGCCAGCC
GCGGCCGAGCGCCGCGCGCTGGTGATCGGCGCCGGTGTCGCGGGCACCTCGATCGCCGAACGGCTCGCGGCGCGCGGCTG
GCAGGTGGAGCTGCTCGACGCCGCCAGCGGCCCCGCCCAGGGCGCGTCCGGCAACCACGCCGGCGTGCTGCGCCCGCTGC
CCAGCCTGGACGACAACCGCATGGGCCGGCTGACACGCGCCGGCACGCTCTACGGCTGGCGCCACATCCAGCGCCTGCAG
GCCGCCGGGCTGCCGCTGCGCGCCGAGGCCTGCGGCGTGCTGCACCTCGCGCGCGACGCCGCGCAGGAGGCGAAGATGCG
CGCGGTGGTGGAACGCCTCGCGCTGCCGCCGGCCCACCTGCGTTTCGTCAGTGCCGCCGAAGCCAGCGAGATCGGCGGCT
GGCCGGTGCCGCTGGGTGGATGGTGGTTTGGCGACAGCGGTTGGGTGCAGCCGCCCAGCCTGTGCGCCGCCAATCTCTCC
GCCGGTGGCGAAGGCATCCGCGCGCACTGGAACGCGCGGGTGACGCTGGCTCGCGCCGACACCCGCTGGCAGGCCCGCGA
TGCGCAGGGCGCGCTGCTTGCAGAAGCGCCGGTCGCCATCCTCGCGGCGGGCACCGGCATCACCGGCTTTCCGCTTGCCG
CGCCCTTGCCGGTGGTGAGCGCGCGCGGCCAGGTGAGCCTGCTGCCGGCCCCCGCTGGCAGCGCGCCCCGGGTGGTGATG
TGCCGCATGGGCTACGTCAGCCCGGCGGTGGATGGCCTGCGCTGCGCCGGCGCCACCTTCGACGTCGGCGACGACGACGC
GACGCTGCGCGCGCGCGACCACCACGAAAACCTGAGCAAGCTCGAAGCCATGCTGCCGGGCTACACCGCCGCGCTCGCCA
CCCAACCGGCGGAAGGCCGCGTCGGCTTCCGCCCGGCCTCGCCCGACCGCCTGCCGATGGTGGGCGCGGTGCCCGCGCTC
ACCACCCTGGCCGCGCCCTGCGCGCTGGCCGAGATTCCCCGCCACACGGGCCTTTACGCACTGTCCGGCTTCGGGGCGCG
CGGCCTGGTGTGGGCGACGCTGGCCGCCGAAACGCTCGCCAGCCAGCTCGACGGGGAACCTCTGCCGCTGGAGCGCGATC
TGGTCGACGCGCTCGATCCCGCCCGCTTCCTGCTGCGCCCCGCACGAACGCTGCGCGGCGAGGACTGA

Upstream 100 bases:

>100_bases
GCTACCCGCGCGGCGGGCCGACGCGCTACCATGGCGCCCCCCGACCGTCCGCGCGCACACCCCCGGCCCTGCCGGCCAGG
TTGCGCGCCCGTGCCGCGCC

Downstream 100 bases:

>100_bases
CGGCCGGCCACCACCCAGAATTCATTACCGCTGCTGACACCTCATGGATGCCATCCCGTTCTTCTTCCTGCTCGGCTTCG
TTGCCCGCCTCGCCCGCTCG

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MPITPASLSFAEDGTPYSTAYGDVYHSSDGGLGQAHHVFLAGNGLPERWRGRERFVIVETGFGLGLNFLASWAAWRKDPQ
RSERLHFVSCELHPFRVEDLALLHARWPEFAPLAAELQANWPCLAPGVHRLHLDGGRVCLTLYFGDARDGLAQLDARADA
FLLDGFSPAKNPDLWSARIFHLLARLAAADATLATWSVAGEVREGLRRAGFEVEKAPGFGGKRQMLRGRYLGRGHRPAPA
AAERRALVIGAGVAGTSIAERLAARGWQVELLDAASGPAQGASGNHAGVLRPLPSLDDNRMGRLTRAGTLYGWRHIQRLQ
AAGLPLRAEACGVLHLARDAAQEAKMRAVVERLALPPAHLRFVSAAEASEIGGWPVPLGGWWFGDSGWVQPPSLCAANLS
AGGEGIRAHWNARVTLARADTRWQARDAQGALLAEAPVAILAAGTGITGFPLAAPLPVVSARGQVSLLPAPAGSAPRVVM
CRMGYVSPAVDGLRCAGATFDVGDDDATLRARDHHENLSKLEAMLPGYTAALATQPAEGRVGFRPASPDRLPMVGAVPAL
TTLAAPCALAEIPRHTGLYALSGFGARGLVWATLAAETLASQLDGEPLPLERDLVDALDPARFLLRPARTLRGED

Sequences:

>Translated_635_residues
MPITPASLSFAEDGTPYSTAYGDVYHSSDGGLGQAHHVFLAGNGLPERWRGRERFVIVETGFGLGLNFLASWAAWRKDPQ
RSERLHFVSCELHPFRVEDLALLHARWPEFAPLAAELQANWPCLAPGVHRLHLDGGRVCLTLYFGDARDGLAQLDARADA
FLLDGFSPAKNPDLWSARIFHLLARLAAADATLATWSVAGEVREGLRRAGFEVEKAPGFGGKRQMLRGRYLGRGHRPAPA
AAERRALVIGAGVAGTSIAERLAARGWQVELLDAASGPAQGASGNHAGVLRPLPSLDDNRMGRLTRAGTLYGWRHIQRLQ
AAGLPLRAEACGVLHLARDAAQEAKMRAVVERLALPPAHLRFVSAAEASEIGGWPVPLGGWWFGDSGWVQPPSLCAANLS
AGGEGIRAHWNARVTLARADTRWQARDAQGALLAEAPVAILAAGTGITGFPLAAPLPVVSARGQVSLLPAPAGSAPRVVM
CRMGYVSPAVDGLRCAGATFDVGDDDATLRARDHHENLSKLEAMLPGYTAALATQPAEGRVGFRPASPDRLPMVGAVPAL
TTLAAPCALAEIPRHTGLYALSGFGARGLVWATLAAETLASQLDGEPLPLERDLVDALDPARFLLRPARTLRGED
>Mature_634_residues
PITPASLSFAEDGTPYSTAYGDVYHSSDGGLGQAHHVFLAGNGLPERWRGRERFVIVETGFGLGLNFLASWAAWRKDPQR
SERLHFVSCELHPFRVEDLALLHARWPEFAPLAAELQANWPCLAPGVHRLHLDGGRVCLTLYFGDARDGLAQLDARADAF
LLDGFSPAKNPDLWSARIFHLLARLAAADATLATWSVAGEVREGLRRAGFEVEKAPGFGGKRQMLRGRYLGRGHRPAPAA
AERRALVIGAGVAGTSIAERLAARGWQVELLDAASGPAQGASGNHAGVLRPLPSLDDNRMGRLTRAGTLYGWRHIQRLQA
AGLPLRAEACGVLHLARDAAQEAKMRAVVERLALPPAHLRFVSAAEASEIGGWPVPLGGWWFGDSGWVQPPSLCAANLSA
GGEGIRAHWNARVTLARADTRWQARDAQGALLAEAPVAILAAGTGITGFPLAAPLPVVSARGQVSLLPAPAGSAPRVVMC
RMGYVSPAVDGLRCAGATFDVGDDDATLRARDHHENLSKLEAMLPGYTAALATQPAEGRVGFRPASPDRLPMVGAVPALT
TLAAPCALAEIPRHTGLYALSGFGARGLVWATLAAETLASQLDGEPLPLERDLVDALDPARFLLRPARTLRGED

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=667, Percent_Identity=37.4812593703148, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_AZOSB (A1K5J4)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932986.1
- ProteinModelPortal:   A1K5J4
- SMR:   A1K5J4
- STRING:   A1K5J4
- GeneID:   4606870
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1482
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A1K5J4
- ProtClustDB:   PRK01747
- BioCyc:   ASP62928:AZO1482-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 67531; Mature: 67399

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITPASLSFAEDGTPYSTAYGDVYHSSDGGLGQAHHVFLAGNGLPERWRGRERFVIVET
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHHCCCCEEEEEEC
GFGLGLNFLASWAAWRKDPQRSERLHFVSCELHPFRVEDLALLHARWPEFAPLAAELQAN
CCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHCC
WPCLAPGVHRLHLDGGRVCLTLYFGDARDGLAQLDARADAFLLDGFSPAKNPDLWSARIF
CCEECCCCEEEEECCCEEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
HLLARLAAADATLATWSVAGEVREGLRRAGFEVEKAPGFGGKRQMLRGRYLGRGHRPAPA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCC
AAERRALVIGAGVAGTSIAERLAARGWQVELLDAASGPAQGASGNHAGVLRPLPSLDDNR
HHCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCC
MGRLTRAGTLYGWRHIQRLQAAGLPLRAEACGVLHLARDAAQEAKMRAVVERLALPPAHL
CCCHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RFVSAAEASEIGGWPVPLGGWWFGDSGWVQPPSLCAANLSAGGEGIRAHWNARVTLARAD
HEEHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCEEEEECEEEEEEECC
TRWQARDAQGALLAEAPVAILAAGTGITGFPLAAPLPVVSARGQVSLLPAPAGSAPRVVM
CCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEE
CRMGYVSPAVDGLRCAGATFDVGDDDATLRARDHHENLSKLEAMLPGYTAALATQPAEGR
EECCCCCCCCCCCEECCCEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHCCCCCCCC
VGFRPASPDRLPMVGAVPALTTLAAPCALAEIPRHTGLYALSGFGARGLVWATLAAETLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
SQLDGEPLPLERDLVDALDPARFLLRPARTLRGED
HHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PITPASLSFAEDGTPYSTAYGDVYHSSDGGLGQAHHVFLAGNGLPERWRGRERFVIVET
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHHCCCCEEEEEEC
GFGLGLNFLASWAAWRKDPQRSERLHFVSCELHPFRVEDLALLHARWPEFAPLAAELQAN
CCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHCC
WPCLAPGVHRLHLDGGRVCLTLYFGDARDGLAQLDARADAFLLDGFSPAKNPDLWSARIF
CCEECCCCEEEEECCCEEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
HLLARLAAADATLATWSVAGEVREGLRRAGFEVEKAPGFGGKRQMLRGRYLGRGHRPAPA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCC
AAERRALVIGAGVAGTSIAERLAARGWQVELLDAASGPAQGASGNHAGVLRPLPSLDDNR
HHCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCC
MGRLTRAGTLYGWRHIQRLQAAGLPLRAEACGVLHLARDAAQEAKMRAVVERLALPPAHL
CCCHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RFVSAAEASEIGGWPVPLGGWWFGDSGWVQPPSLCAANLSAGGEGIRAHWNARVTLARAD
HEEHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCEEEEECEEEEEEECC
TRWQARDAQGALLAEAPVAILAAGTGITGFPLAAPLPVVSARGQVSLLPAPAGSAPRVVM
CCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEE
CRMGYVSPAVDGLRCAGATFDVGDDDATLRARDHHENLSKLEAMLPGYTAALATQPAEGR
EECCCCCCCCCCCEECCCEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHCCCCCCCC
VGFRPASPDRLPMVGAVPALTTLAAPCALAEIPRHTGLYALSGFGARGLVWATLAAETLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
SQLDGEPLPLERDLVDALDPARFLLRPARTLRGED
HHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA