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The map label for this gene is gltX

Identifier: 119897770

GI number: 119897770

Start: 1612279

End: 1613682

Strand: Direct

Name: gltX

Synonym: azo1479

Alternate gene names: 119897770

Gene position: 1612279-1613682 (Clockwise)

Preceding gene: 119897766

Following gene: 119897776

Centisome position: 36.84

GC content: 68.09

Gene sequence:

>1404_bases
ATGGCCGCCACCCCAGTCCGTACCCGTTTCGCGCCCAGCCCCACCGGCTACCTCCACATCGGCGGTGCCCGCACGGCGCT
GTTCTCCTGGGCCTATGCCCGCCGCCACGGCGGCCGCTTCATCCTGCGCATTGAGGACACCGACGTGGCCCGCTCCACCC
CGGAAGCGGTGCAGGCCATCCTGGATGGCATGAAGTGGCTCGGCCTGGAGCATGACGAGGGCCCGTTCTACCAGATGCAG
CGCATGGATCGCTACAAGGAAGTGATCCAGCAGATGCTCGCCGCCGGCACCGCCTACTACTGCTACACCTCGAAGGAAGA
ACTCGACGCGCTGCGCGCCGAGCAGGAAGCGAAGAAGGAGAAGCCGCGCTACGACGGCCGCTGGCGCCCCGCGCCCGGCA
AGACGCTGCCCACGCCGCCGGCCGGCGTGCAGCCGGTGGTGCGCTTCTGCAACCCGACCACCGGCGTGGTGGCCTGGGAC
GACCTGGTGAAGGGCCGCATCGAGATCTCCAACACCGAACTCGACGACTTCATCATCGCGCGCGCCGACGGCACGCCCAC
CTACAACTTTTGCGTCGTCGTCGATGACTGGGACATGGGCATCACCCAGGTCATCCGCGGCGACGACCATGTGAACAACA
CCCCGCGCCAGATCAACGTGCTGCAGGCGCTTGGCGCCAGCGTGCCGCAGTACGCGCACCTGTCGATGATCCTGGGCGAC
GACGGCACCAAGCTCTCCAAGCGCCACGGCGCGGTGAGCGTGATGCAGTACGACGACGAAGGCTATCTGCCGGAGGCCGT
CATCAACTACCTCGCGCGCCTGGGCTGGAGCCACGGCGACGACGAGATCTTCAGCCGTGAGCAGTTCGTCGAGTGGTTCG
ACCTCGACCACATCACGCCGTCGGCGGCGCAGTTCAACACCGAAAAGCTCAACTGGCTGAACGCCCACTACATCAAGCAG
GCCGACAACGCCTACCTGGCGGCCGAAGTCGCCAACCGGCTTGCCCGCCGCGGGGTCGATCCGGAAGCCGGCCCGGCGCT
GGAGCAGGTGGTGGCGCTGTACAAGGACCGCTCCGCCAACCTCAACGAACTCGCCGACGCGGCCGAATTGTTCTGCGTGG
ATGTCCATGCCGCGCCGGAAGTGATCGCCCAGCACCTCACCGACACCGCCAGGGCGGCGCTCGCCAGCTTGCGCGCCCGC
TTCGAGGCGGTGGCGTGGGACAAGGCCGCGCTCAACCAGGCGATCAAGGACACCATGGCCGAGCACGGCCTGAAGATGCC
GCAGGTGGCGATTCCGCTGCGGGTGGCGCTGCTCGGCGTGCCGCAGACGCCTTCGATCGACGCGGTGGTGGAGGTGCTCG
GCCGCGAGCGTGTGCTGGCGCGGCTGGCGCGACACCTCGGCTGA

Upstream 100 bases:

>100_bases
ATGTACATGGCTGTTCCCTGCGCGGCGCCTGCTGCGCCGCAATGTCGCGACGATCGCTTAGAATTCCGCCTTTCGTCCTG
TCCGCATTCCGAGTCCCACG

Downstream 100 bases:

>100_bases
GGTCCGGCCTTCGCGCTGGTCGCAACGCGGCGCCCGCTCCCGCCAGGGAGGGGCGCCGCGGCGTTTTCAGCGGACGAACT
TTTCCGGGCGCAGCACGCCC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAATPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGRFILRIEDTDVARSTPEAVQAILDGMKWLGLEHDEGPFYQMQ
RMDRYKEVIQQMLAAGTAYYCYTSKEELDALRAEQEAKKEKPRYDGRWRPAPGKTLPTPPAGVQPVVRFCNPTTGVVAWD
DLVKGRIEISNTELDDFIIARADGTPTYNFCVVVDDWDMGITQVIRGDDHVNNTPRQINVLQALGASVPQYAHLSMILGD
DGTKLSKRHGAVSVMQYDDEGYLPEAVINYLARLGWSHGDDEIFSREQFVEWFDLDHITPSAAQFNTEKLNWLNAHYIKQ
ADNAYLAAEVANRLARRGVDPEAGPALEQVVALYKDRSANLNELADAAELFCVDVHAAPEVIAQHLTDTARAALASLRAR
FEAVAWDKAALNQAIKDTMAEHGLKMPQVAIPLRVALLGVPQTPSIDAVVEVLGRERVLARLARHLG

Sequences:

>Translated_467_residues
MAATPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGRFILRIEDTDVARSTPEAVQAILDGMKWLGLEHDEGPFYQMQ
RMDRYKEVIQQMLAAGTAYYCYTSKEELDALRAEQEAKKEKPRYDGRWRPAPGKTLPTPPAGVQPVVRFCNPTTGVVAWD
DLVKGRIEISNTELDDFIIARADGTPTYNFCVVVDDWDMGITQVIRGDDHVNNTPRQINVLQALGASVPQYAHLSMILGD
DGTKLSKRHGAVSVMQYDDEGYLPEAVINYLARLGWSHGDDEIFSREQFVEWFDLDHITPSAAQFNTEKLNWLNAHYIKQ
ADNAYLAAEVANRLARRGVDPEAGPALEQVVALYKDRSANLNELADAAELFCVDVHAAPEVIAQHLTDTARAALASLRAR
FEAVAWDKAALNQAIKDTMAEHGLKMPQVAIPLRVALLGVPQTPSIDAVVEVLGRERVLARLARHLG
>Mature_466_residues
AATPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGRFILRIEDTDVARSTPEAVQAILDGMKWLGLEHDEGPFYQMQR
MDRYKEVIQQMLAAGTAYYCYTSKEELDALRAEQEAKKEKPRYDGRWRPAPGKTLPTPPAGVQPVVRFCNPTTGVVAWDD
LVKGRIEISNTELDDFIIARADGTPTYNFCVVVDDWDMGITQVIRGDDHVNNTPRQINVLQALGASVPQYAHLSMILGDD
GTKLSKRHGAVSVMQYDDEGYLPEAVINYLARLGWSHGDDEIFSREQFVEWFDLDHITPSAAQFNTEKLNWLNAHYIKQA
DNAYLAAEVANRLARRGVDPEAGPALEQVVALYKDRSANLNELADAAELFCVDVHAAPEVIAQHLTDTARAALASLRARF
EAVAWDKAALNQAIKDTMAEHGLKMPQVAIPLRVALLGVPQTPSIDAVVEVLGRERVLARLARHLG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=31.0975609756098, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=55.0755939524838, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI226510925, Length=292, Percent_Identity=34.5890410958904, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786895, Length=110, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=467, Percent_Identity=29.3361884368308, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=30.2040816326531, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=114, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=232, Percent_Identity=27.1551724137931, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=31.8637274549098, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI17864488, Length=120, Percent_Identity=30.8333333333333, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_AZOSB (A1K5J1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932983.1
- ProteinModelPortal:   A1K5J1
- SMR:   A1K5J1
- STRING:   A1K5J1
- GeneID:   4606868
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1479
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A1K5J1
- ProtClustDB:   PRK01406
- BioCyc:   ASP62928:AZO1479-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51866; Mature: 51735

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 247-247

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGRFILRIEDTDVARSTPEAVQAI
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
LDGMKWLGLEHDEGPFYQMQRMDRYKEVIQQMLAAGTAYYCYTSKEELDALRAEQEAKKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHC
KPRYDGRWRPAPGKTLPTPPAGVQPVVRFCNPTTGVVAWDDLVKGRIEISNTELDDFIIA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHCCEEEECCCCCCCEEEE
RADGTPTYNFCVVVDDWDMGITQVIRGDDHVNNTPRQINVLQALGASVPQYAHLSMILGD
ECCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECC
DGTKLSKRHGAVSVMQYDDEGYLPEAVINYLARLGWSHGDDEIFSREQFVEWFDLDHITP
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SAAQFNTEKLNWLNAHYIKQADNAYLAAEVANRLARRGVDPEAGPALEQVVALYKDRSAN
CHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
LNELADAAELFCVDVHAAPEVIAQHLTDTARAALASLRARFEAVAWDKAALNQAIKDTMA
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHGLKMPQVAIPLRVALLGVPQTPSIDAVVEVLGRERVLARLARHLG
HCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AATPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGRFILRIEDTDVARSTPEAVQAI
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
LDGMKWLGLEHDEGPFYQMQRMDRYKEVIQQMLAAGTAYYCYTSKEELDALRAEQEAKKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHC
KPRYDGRWRPAPGKTLPTPPAGVQPVVRFCNPTTGVVAWDDLVKGRIEISNTELDDFIIA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHCCEEEECCCCCCCEEEE
RADGTPTYNFCVVVDDWDMGITQVIRGDDHVNNTPRQINVLQALGASVPQYAHLSMILGD
ECCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECC
DGTKLSKRHGAVSVMQYDDEGYLPEAVINYLARLGWSHGDDEIFSREQFVEWFDLDHITP
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SAAQFNTEKLNWLNAHYIKQADNAYLAAEVANRLARRGVDPEAGPALEQVVALYKDRSAN
CHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
LNELADAAELFCVDVHAAPEVIAQHLTDTARAALASLRARFEAVAWDKAALNQAIKDTMA
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHGLKMPQVAIPLRVALLGVPQTPSIDAVVEVLGRERVLARLARHLG
HCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA