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The map label for this gene is greB [H]

Identifier: 119897715

GI number: 119897715

Start: 1554874

End: 1555542

Strand: Direct

Name: greB [H]

Synonym: azo1424

Alternate gene names: 119897715

Gene position: 1554874-1555542 (Clockwise)

Preceding gene: 119897710

Following gene: 119897716

Centisome position: 35.53

GC content: 65.32

Gene sequence:

>669_bases
ATGGTATGCGCCGGTCCGCCGAATGACAACTTCACCGGCATGGCTTATCGAGTGTGCGGCGCGAGGCGGCTGCAGTCTTG
CCATAATTGGAGCATCGAGAGTGCGGAGACGCAGGTGAACAAGGCATTCGTGAAGGAAGGCGAGGGGCAGGACGACGACG
AGGAGTTGTCGCCGTCGTTGCGCTTGCCCCCGGGCAGCAAGAACTACATGACGCGGCGCGGCTACGACGCGCTGCGGACC
GAACTCGATCAACTGGTGCGGGTGGAGCGCCCCAAGCTGGTCGAAACGGTCGCGTGGGCGGCGGGCAATGGCGACCGCTC
GGAGAACGGCGACTACATCTACGGCAAGAAGCGGCTGCGCGAGATCGACCGCCGCATCCGCTTCCTGATCAAGCGTATCG
AGAGCGCCGAGGTGGTCGACCCCGAGCGCCAGGAGAATGTCGACCAGGTCTTCTTCGGCGCCACAGTGACGATCACCGAC
GCCGACGGCGAGGACGAGCAGACCTGGCAGATCGTCGGCGTGGACGAGGCGAACGCGTCGGAAGGGCGGATCAGCTGGAT
CTCGCCGCTGGCGCGCGCGCTGCTGAAGGTGAAGCTGGGCGAGACGGTGCGCTTCATGAGCCCGGCCGGGCCACGCGAGA
TCGAGGTGGTCGATATCCGCTACGTTTGA

Upstream 100 bases:

>100_bases
CGGGCGTGCAGGCTGGTCACTTCATCGAAAGCGTCGTCGTTCATCGAGGGCGGGCTCGGCGGGGCGGCAAGTCCTTGCCG
GGGTGAGTCGGGTGCCCGCA

Downstream 100 bases:

>100_bases
CCCGGCGCGGCGGCGGGGCTTGAAATCGGCGCGGGCATCCCCACATGGGTGGGCGTCGATTTCAAAAACACGGAGCCGCA
TCCATGTCCGAAGCGCAAGC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MVCAGPPNDNFTGMAYRVCGARRLQSCHNWSIESAETQVNKAFVKEGEGQDDDEELSPSLRLPPGSKNYMTRRGYDALRT
ELDQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLREIDRRIRFLIKRIESAEVVDPERQENVDQVFFGATVTITD
ADGEDEQTWQIVGVDEANASEGRISWISPLARALLKVKLGETVRFMSPAGPREIEVVDIRYV

Sequences:

>Translated_222_residues
MVCAGPPNDNFTGMAYRVCGARRLQSCHNWSIESAETQVNKAFVKEGEGQDDDEELSPSLRLPPGSKNYMTRRGYDALRT
ELDQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLREIDRRIRFLIKRIESAEVVDPERQENVDQVFFGATVTITD
ADGEDEQTWQIVGVDEANASEGRISWISPLARALLKVKLGETVRFMSPAGPREIEVVDIRYV
>Mature_222_residues
MVCAGPPNDNFTGMAYRVCGARRLQSCHNWSIESAETQVNKAFVKEGEGQDDDEELSPSLRLPPGSKNYMTRRGYDALRT
ELDQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLREIDRRIRFLIKRIESAEVVDPERQENVDQVFFGATVTITD
ADGEDEQTWQIVGVDEANASEGRISWISPLARALLKVKLGETVRFMSPAGPREIEVVDIRYV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=48.3660130718954, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=37.2549019607843, Blast_Score=103, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 25050; Mature: 25050

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCAGPPNDNFTGMAYRVCGARRLQSCHNWSIESAETQVNKAFVKEGEGQDDDEELSPSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCE
RLPPGSKNYMTRRGYDALRTELDQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLR
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHH
EIDRRIRFLIKRIESAEVVDPERQENVDQVFFGATVTITDADGEDEQTWQIVGVDEANAS
HHHHHHHHHHHHHCCCCCCCCHHHCCHHHEEECCEEEEECCCCCCCCEEEEEECCCCCCC
EGRISWISPLARALLKVKLGETVRFMSPAGPREIEVVDIRYV
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MVCAGPPNDNFTGMAYRVCGARRLQSCHNWSIESAETQVNKAFVKEGEGQDDDEELSPSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCE
RLPPGSKNYMTRRGYDALRTELDQLVRVERPKLVETVAWAAGNGDRSENGDYIYGKKRLR
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHH
EIDRRIRFLIKRIESAEVVDPERQENVDQVFFGATVTITDADGEDEQTWQIVGVDEANAS
HHHHHHHHHHHHHCCCCCCCCHHHCCHHHEEECCEEEEECCCCCCCCEEEEEECCCCCCC
EGRISWISPLARALLKVKLGETVRFMSPAGPREIEVVDIRYV
CCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]