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The map label for this gene is nuoB2

Identifier: 119897688

GI number: 119897688

Start: 1525357

End: 1525833

Strand: Direct

Name: nuoB2

Synonym: azo1397

Alternate gene names: 119897688

Gene position: 1525357-1525833 (Clockwise)

Preceding gene: 119897687

Following gene: 119897689

Centisome position: 34.86

GC content: 59.12

Gene sequence:

>477_bases
ATGAGCATTGAGGGCGTCTTTCGCGAAGGGTTCGTCACTACTTCGCTCGATGCGGTCATCAATTGGACGCGAACCGGCTC
GTTGTGGCCGATGACCTTCGGGTTGGCCTGTTGTGCGGTTGAAATGATTCACGCGGGTTGTTCCCGCTACGATCTGGACC
GTTTCGGTGTGGTGTTCCGCCCCAGCCCGCGGCAATCCGATTTGATGATCGTCGCCGGCACGCTGTGCAACAAGATGGCT
CCGGCGCTGCGCAAGGTGTACGACCAGATGGCCGAGCCGCGCTGGGTTATCTCCATGGGGTCCTGTGCGAATGGTGGCGG
CTATTACCACTATTCCTACTCGGTCGTGCGCGGCTGCGACCGGATTGTTCCCGTTGATGTCTATGTGCCTGGCTGTCCGC
CGACGGCCGAGGCATTGTTGTACGGCATTATTCAGCTGCAGAACAAGATCAAGCGGACCAACACGATTGCCCGCTGA

Upstream 100 bases:

>100_bases
GCGTGGTTTGTTTTCGGTTCGGTGATGGTCTTCCTGGCTATTCTGGTCATCGGTTACATCGTCGAGTGGAAAAACGGCGC
ACTCGACTGGGAGTAATGCA

Downstream 100 bases:

>100_bases
TTGACGGATGCCCAGACATGAGTGCCAAGCTTGAACGCCTGAGTCAAACGCTTCGCGATGTCCTCGGTGATGTTGTTGAG
TCGCTGGTGGTGGATCGCGG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIHAGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR

Sequences:

>Translated_158_residues
MSIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIHAGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR
>Mature_157_residues
SIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIHAGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=78.5234899328859, Blast_Score=258, Evalue=2e-69,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=50.3311258278146, Blast_Score=165, Evalue=1e-42,
Organism=Escherichia coli, GI1789074, Length=107, Percent_Identity=43.9252336448598, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788834, Length=126, Percent_Identity=38.0952380952381, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=72.5352112676056, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=78.7234042553192, Blast_Score=255, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=78.7234042553192, Blast_Score=255, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=76.7123287671233, Blast_Score=250, Evalue=3e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB1_AZOSB (A1K5A9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932901.1
- ProteinModelPortal:   A1K5A9
- SMR:   A1K5A9
- STRING:   A1K5A9
- GeneID:   4609418
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1397
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   A1K5A9
- ProtClustDB:   PRK06411
- BioCyc:   ASP62928:AZO1397-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17511; Mature: 17379

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIHAGCSRYDLDRFGVVFR
CCCCCCHHCCCCCEEHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEC
PSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIHAGCSRYDLDRFGVVFR
CCCCCHHCCCCCEEHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEC
PSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA