Click here to switch to the map view.

The map label for this gene is fixJ2 [H]

Identifier: 119897667

GI number: 119897667

Start: 1503974

End: 1504612

Strand: Direct

Name: fixJ2 [H]

Synonym: azo1376

Alternate gene names: 119897667

Gene position: 1503974-1504612 (Clockwise)

Preceding gene: 119897666

Following gene: 119897668

Centisome position: 34.37

GC content: 65.26

Gene sequence:

>639_bases
GTGAGCGTATTGCCCGTCGCGCCTGATCAGGAAATCTTCATCGTCGATGACGACGATGCCTTGCGCGATTCCCTGGTGTG
GATGCTGGAATCCAGCGGCTACAAGGTTGTCGCTTTCGACTCCGCCGAAGCCTTCCTGCACGCGTGGCACGAGGGCCTGG
CCGGCTGCCTGGTGCTCGACGTGCGCATGCCGGGCATGAGCGGACTGGAACTGTTCGAGGAGCTGGGGCGGCGGCGCTGC
ACGCTCCCGGTGATCTTCATCACCGGCCATGGCGATGTTCCGATGGCGGTGGCGGCGCTGAAGAAGGGCGCCGCCGACTT
CATCGAAAAGCCCTTTGCCGACCGCGAGATGCTCGCCCTCATCGAGCACTGCCTGCAGCAGGAGCGGGCCAGCCGCGACA
AACGCCGTCAGGAGGCCGATACCGCACGCCGGCTGGAGCATCTGACCCAGCGCGAGCGCGAGGTGCTCGATCTCATCATC
GCCGGCAAGCTCAACAAGCAGATCGCCGATGTGCTGGGCATCAGCATCAAGACCGTCGAGGTCCATCGCGCGCGGGTGAT
GGAAAAGATGGGGGCTCATTCGCTGGCCGAGCTTGTCCAGCACGTGGTGACGGTCGAGCCCGCGGCCTCGGTCCGCTGA

Upstream 100 bases:

>100_bases
TGCCGGTCGATCATCGAGTTCCACGATGGTCGTCTGGTTGTCGACGGCAACCCCGAAGGCGGTACCATATTTTCCTTCAC
CCTGCCCACGGAGGCCGCCA

Downstream 100 bases:

>100_bases
GGCGCTCGCTGGGGCACGGCGAGACAACGCACGCAACGCCCTGATAGTGCGATAAAATTGCGAGCTTTTTTCGAGGCACT
CCCTATGAGCGCTCGCATTA

Product: transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSVLPVAPDQEIFIVDDDDALRDSLVWMLESSGYKVVAFDSAEAFLHAWHEGLAGCLVLDVRMPGMSGLELFEELGRRRC
TLPVIFITGHGDVPMAVAALKKGAADFIEKPFADREMLALIEHCLQQERASRDKRRQEADTARRLEHLTQREREVLDLII
AGKLNKQIADVLGISIKTVEVHRARVMEKMGAHSLAELVQHVVTVEPAASVR

Sequences:

>Translated_212_residues
MSVLPVAPDQEIFIVDDDDALRDSLVWMLESSGYKVVAFDSAEAFLHAWHEGLAGCLVLDVRMPGMSGLELFEELGRRRC
TLPVIFITGHGDVPMAVAALKKGAADFIEKPFADREMLALIEHCLQQERASRDKRRQEADTARRLEHLTQREREVLDLII
AGKLNKQIADVLGISIKTVEVHRARVMEKMGAHSLAELVQHVVTVEPAASVR
>Mature_211_residues
SVLPVAPDQEIFIVDDDDALRDSLVWMLESSGYKVVAFDSAEAFLHAWHEGLAGCLVLDVRMPGMSGLELFEELGRRRCT
LPVIFITGHGDVPMAVAALKKGAADFIEKPFADREMLALIEHCLQQERASRDKRRQEADTARRLEHLTQREREVLDLIIA
GKLNKQIADVLGISIKTVEVHRARVMEKMGAHSLAELVQHVVTVEPAASVR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=34.6774193548387, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788905, Length=132, Percent_Identity=34.0909090909091, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788222, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786747, Length=151, Percent_Identity=25.1655629139073, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=149, Percent_Identity=27.5167785234899, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=30.5084745762712, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=30.9278350515464, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=28.8135593220339, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788712, Length=147, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=32.5, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=32.1739130434783, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23630; Mature: 23498

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLPVAPDQEIFIVDDDDALRDSLVWMLESSGYKVVAFDSAEAFLHAWHEGLAGCLVLD
CCCCCCCCCCEEEEECCCHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHEEEEEE
VRMPGMSGLELFEELGRRRCTLPVIFITGHGDVPMAVAALKKGAADFIEKPFADREMLAL
EECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IEHCLQQERASRDKRRQEADTARRLEHLTQREREVLDLIIAGKLNKQIADVLGISIKTVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
VHRARVMEKMGAHSLAELVQHVVTVEPAASVR
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SVLPVAPDQEIFIVDDDDALRDSLVWMLESSGYKVVAFDSAEAFLHAWHEGLAGCLVLD
CCCCCCCCCEEEEECCCHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHEEEEEE
VRMPGMSGLELFEELGRRRCTLPVIFITGHGDVPMAVAALKKGAADFIEKPFADREMLAL
EECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IEHCLQQERASRDKRRQEADTARRLEHLTQREREVLDLIIAGKLNKQIADVLGISIKTVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
VHRARVMEKMGAHSLAELVQHVVTVEPAASVR
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]