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The map label for this gene is folD

Identifier: 119897668

GI number: 119897668

Start: 1504697

End: 1505572

Strand: Direct

Name: folD

Synonym: azo1377

Alternate gene names: 119897668

Gene position: 1504697-1505572 (Clockwise)

Preceding gene: 119897667

Following gene: 119897669

Centisome position: 34.38

GC content: 66.89

Gene sequence:

>876_bases
ATGAGCGCTCGCATTATCGATGGCAACGCCCTGGCGGCGCGCGTGCGTGGCGAAATTGCCGCGCGTGCGGCGGCGCTCAC
GGATCGCGGCACCCAGCCGTGCCTCGCGGTTCTACTCGTCGGCGAAAATCCGGCATCGGCGGTCTATGTGCGCAGCAAGG
TCGCTGCGTGCGAGAAGGCCGGCATCCGCTCGCTGCGCTTCGACTATCCGCAGGACGTCGATCCCGCGGTCGTCATGGAG
AAGCTGGCCGCGCTCAACGCCGATCCGTCGGTGCATGGCATCCTCGTGCAACTGCCGCTGCCGCCGCAGTTCGACGAGGC
GGCAGTGTTGGAGGCGATCAGCGTCGCCAAGGATGTCGACGGCTTTCATGCCGAGAATGTCGGCCGTCTGTCGCAGAACC
AGGAAGCCTTTCTGCCCTGTACTCCCCACGGCGTCATGAAGATGCTGGAAGCCGAGGGCGTTCCGCTTGCCGGCGCAGAG
GCGGTGGTGATCGGCCGCTCCAACATCGTCGGCAAGCCGATGGCCATGTTGCTGACCAACGCCGGCGCCACCGTCACCGT
GTGCCACTCGAGGACGCGCGACCTGCGCTTCCATACCGGCCGCGCCGACATCGTGGTTGCTGCCATCGGCAAGCCGCGCT
TCGTCACCGGCGACATGCTCAAGCCGGGTGCGACGGTGATCGACGTCGGCATCAACCGCATCACCGACGGACCGGAAGCG
GGCAAGCTCTGCGGCGATGTGGATTTCGCGTCTGCCAGCGAGGTGGCCGGGCTGATTACGCCAGTGCCGGGCGGGGTGGG
GCCGATGACCATCACCATGCTGCTCGAAAACACCGTGATTTCGGCCGAGCGCAGCGCCCGCGCTGCGGGCAAGTGA

Upstream 100 bases:

>100_bases
GGCCTCGGTCCGCTGAGGCGCTCGCTGGGGCACGGCGAGACAACGCACGCAACGCCCTGATAGTGCGATAAAATTGCGAG
CTTTTTTCGAGGCACTCCCT

Downstream 100 bases:

>100_bases
GGCGGGCATGAGCGAACAGCAGACGTCCGCCGCGCCCGTCGTCGGAATCATCATGGGCTCGAACTCCGACTGGCCCACCA
TGCAGGCAGCCGCCAAGATC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSARIIDGNALAARVRGEIAARAAALTDRGTQPCLAVLLVGENPASAVYVRSKVAACEKAGIRSLRFDYPQDVDPAVVME
KLAALNADPSVHGILVQLPLPPQFDEAAVLEAISVAKDVDGFHAENVGRLSQNQEAFLPCTPHGVMKMLEAEGVPLAGAE
AVVIGRSNIVGKPMAMLLTNAGATVTVCHSRTRDLRFHTGRADIVVAAIGKPRFVTGDMLKPGATVIDVGINRITDGPEA
GKLCGDVDFASASEVAGLITPVPGGVGPMTITMLLENTVISAERSARAAGK

Sequences:

>Translated_291_residues
MSARIIDGNALAARVRGEIAARAAALTDRGTQPCLAVLLVGENPASAVYVRSKVAACEKAGIRSLRFDYPQDVDPAVVME
KLAALNADPSVHGILVQLPLPPQFDEAAVLEAISVAKDVDGFHAENVGRLSQNQEAFLPCTPHGVMKMLEAEGVPLAGAE
AVVIGRSNIVGKPMAMLLTNAGATVTVCHSRTRDLRFHTGRADIVVAAIGKPRFVTGDMLKPGATVIDVGINRITDGPEA
GKLCGDVDFASASEVAGLITPVPGGVGPMTITMLLENTVISAERSARAAGK
>Mature_290_residues
SARIIDGNALAARVRGEIAARAAALTDRGTQPCLAVLLVGENPASAVYVRSKVAACEKAGIRSLRFDYPQDVDPAVVMEK
LAALNADPSVHGILVQLPLPPQFDEAAVLEAISVAKDVDGFHAENVGRLSQNQEAFLPCTPHGVMKMLEAEGVPLAGAEA
VVIGRSNIVGKPMAMLLTNAGATVTVCHSRTRDLRFHTGRADIVVAAIGKPRFVTGDMLKPGATVIDVGINRITDGPEAG
KLCGDVDFASASEVAGLITPVPGGVGPMTITMLLENTVISAERSARAAGK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=45.0847457627119, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=43.1034482758621, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=45.6375838926174, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=51.7857142857143, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=39.2491467576792, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=45.1388888888889, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=305, Percent_Identity=42.9508196721311, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=293, Percent_Identity=28.6689419795222, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=46.7796610169492, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=46.7796610169492, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=47.0588235294118, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=47.0588235294118, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=47.0588235294118, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=47.0588235294118, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_AZOSB (A1K589)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932881.1
- ProteinModelPortal:   A1K589
- SMR:   A1K589
- STRING:   A1K589
- GeneID:   4608767
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1377
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   A1K589
- ProtClustDB:   PRK14189
- BioCyc:   ASP62928:AZO1377-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30187; Mature: 30056

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARIIDGNALAARVRGEIAARAAALTDRGTQPCLAVLLVGENPASAVYVRSKVAACEKA
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHH
GIRSLRFDYPQDVDPAVVMEKLAALNADPSVHGILVQLPLPPQFDEAAVLEAISVAKDVD
CCCCEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCC
GFHAENVGRLSQNQEAFLPCTPHGVMKMLEAEGVPLAGAEAVVIGRSNIVGKPMAMLLTN
CCCCCCCHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHEEEC
AGATVTVCHSRTRDLRFHTGRADIVVAAIGKPRFVTGDMLKPGATVIDVGINRITDGPEA
CCCEEEEEECCCCCEEEECCCCEEEEEECCCCCEEECCCCCCCCEEEECCCHHCCCCCCC
GKLCGDVDFASASEVAGLITPVPGGVGPMTITMLLENTVISAERSARAAGK
CCCCCCCCCCCHHHHCEEEECCCCCCCHHEEEEEHHHHHHHCHHHHHCCCC
>Mature Secondary Structure 
SARIIDGNALAARVRGEIAARAAALTDRGTQPCLAVLLVGENPASAVYVRSKVAACEKA
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHH
GIRSLRFDYPQDVDPAVVMEKLAALNADPSVHGILVQLPLPPQFDEAAVLEAISVAKDVD
CCCCEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCC
GFHAENVGRLSQNQEAFLPCTPHGVMKMLEAEGVPLAGAEAVVIGRSNIVGKPMAMLLTN
CCCCCCCHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHEEEC
AGATVTVCHSRTRDLRFHTGRADIVVAAIGKPRFVTGDMLKPGATVIDVGINRITDGPEA
CCCEEEEEECCCCCEEEECCCCEEEEEECCCCCEEECCCCCCCCEEEECCCHHCCCCCCC
GKLCGDVDFASASEVAGLITPVPGGVGPMTITMLLENTVISAERSARAAGK
CCCCCCCCCCCHHHHCEEEECCCCCCCHHEEEEEHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA