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The map label for this gene is mltB [H]

Identifier: 119897628

GI number: 119897628

Start: 1455298

End: 1456512

Strand: Direct

Name: mltB [H]

Synonym: azo1337

Alternate gene names: 119897628

Gene position: 1455298-1456512 (Clockwise)

Preceding gene: 119897619

Following gene: 119897629

Centisome position: 33.26

GC content: 71.28

Gene sequence:

>1215_bases
ATGACCACGCCCCGTTCCAAGTCCCGTTTTCCCGTTTCCGCCCTGCTGGCTGTCGCGCCGTTCGCGCTCTCGCTGGCGGC
ACCCGCGGCGGCGCAGGACCTCGCTTGCCTGGCCCAGCTTCGCGCGCCCGCGCAGGCCTACGGCGTTTCCGCGGCAACCT
TCGACCGGCTCACCGCCGGCCTGCAGGCCGACCCCACGGTGCTGCCTTTGCTGGATGCCCAGCCGGAATTCGTGACCCCG
ATCTGGGACTACATGGCCGCCCTGGTGGACGATGAACGCATCGCCGACGGGCGGGCTATGCTCGAGCAATGGTCTGCGGT
GCTGGCGCGGGTGGAGGCGGAGTACGGGGTGGATGCCGCAACAGTGGTCGCGGTGTGGGGGGTGGAAAGCAATTACGGGC
GCAACTTCGGAAGCCGCGCGCTGCTGCCGTCGCTCGCGACCCTGGCCTGCAACGGGCGCCGCCAGTCCTTCTTCCGCCAG
GAGTTGTTCGCAACGATGAAGATCGTCGAGGACGGCCATGTGGCGCCGGAGCGGCTGAACGGGTCGTGGGCGGGGGCCTT
CGGTCACACCCAGTTCATGCCCTCCACCTATCTGCGGCTGGCGGTGGATTTCGACGGCGACGGCCGCCGCGACCTGGTGG
ACAGCATTGCCGATGCGCTGGCATCGACTGCCAACTTCCTGCGTCAGGCCGGCTGGCAGCGCGGCCTGGCATGGGGTTAC
GAGGTAGGGCTGCCGGCGGGGTTCGACAGCTCGGGCGCGGGGCGCAAGAACAAGCGCGGCATCGCCGAGTGGGCCGGCCG
CGGCGTCCGCCGTGCCGACGGCGGGGCGCTTGCGCAAGCAGGCAATGCGGCGCTGTTGCTGCCGGCAGGCGCGAGCGGCC
CGGCCTTCCTCGTCACGCGCAATTTCGACGCGATCTACAGTTACAACGCCGCCGAGAGCTACGCGCTTGCGATCGCCCAC
CTGTCGGACCGGCTGCGAGGCGCGGGCCCGTTTGCGACGCCCTGGCCGACGGACGATCCCGGCCTGTCGCGCGCCGAGCG
GCGCGAGGTGCAGACGCTGCTGCTCGCCCGTGGTCACGACATCGGCAATCCGGATGGCATGATCGGCGCGCGCACGCGCG
AGGCGCTGCGCGTCGTCCAGGCCGAATTCGGACTGCCGGCGGACGGCCGTGCGGGGCGCCGCGTGCTGGACGCCCTGCGG
CAGGCGCGGCCGTAA

Upstream 100 bases:

>100_bases
AATGCGCAAATTCTGAAATGAGCGGTTTCGAGCGCGTGCGCGGCGGCCAGGATGGCTGCCGGTATACTTTTGCGCTCTGC
AACGCCGCTTCCGCCCCGCC

Downstream 100 bases:

>100_bases
TCGGGTGCTGGATCACCGCCGTCCGGTCTATTGACGTTTACGTCAACGTAAACTATTTTCCCCGCATCCATGACTGATGC
GGAGGAGAGACGGATGAAAA

Product: peptidoglycan-binding protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTTPRSKSRFPVSALLAVAPFALSLAAPAAAQDLACLAQLRAPAQAYGVSAATFDRLTAGLQADPTVLPLLDAQPEFVTP
IWDYMAALVDDERIADGRAMLEQWSAVLARVEAEYGVDAATVVAVWGVESNYGRNFGSRALLPSLATLACNGRRQSFFRQ
ELFATMKIVEDGHVAPERLNGSWAGAFGHTQFMPSTYLRLAVDFDGDGRRDLVDSIADALASTANFLRQAGWQRGLAWGY
EVGLPAGFDSSGAGRKNKRGIAEWAGRGVRRADGGALAQAGNAALLLPAGASGPAFLVTRNFDAIYSYNAAESYALAIAH
LSDRLRGAGPFATPWPTDDPGLSRAERREVQTLLLARGHDIGNPDGMIGARTREALRVVQAEFGLPADGRAGRRVLDALR
QARP

Sequences:

>Translated_404_residues
MTTPRSKSRFPVSALLAVAPFALSLAAPAAAQDLACLAQLRAPAQAYGVSAATFDRLTAGLQADPTVLPLLDAQPEFVTP
IWDYMAALVDDERIADGRAMLEQWSAVLARVEAEYGVDAATVVAVWGVESNYGRNFGSRALLPSLATLACNGRRQSFFRQ
ELFATMKIVEDGHVAPERLNGSWAGAFGHTQFMPSTYLRLAVDFDGDGRRDLVDSIADALASTANFLRQAGWQRGLAWGY
EVGLPAGFDSSGAGRKNKRGIAEWAGRGVRRADGGALAQAGNAALLLPAGASGPAFLVTRNFDAIYSYNAAESYALAIAH
LSDRLRGAGPFATPWPTDDPGLSRAERREVQTLLLARGHDIGNPDGMIGARTREALRVVQAEFGLPADGRAGRRVLDALR
QARP
>Mature_403_residues
TTPRSKSRFPVSALLAVAPFALSLAAPAAAQDLACLAQLRAPAQAYGVSAATFDRLTAGLQADPTVLPLLDAQPEFVTPI
WDYMAALVDDERIADGRAMLEQWSAVLARVEAEYGVDAATVVAVWGVESNYGRNFGSRALLPSLATLACNGRRQSFFRQE
LFATMKIVEDGHVAPERLNGSWAGAFGHTQFMPSTYLRLAVDFDGDGRRDLVDSIADALASTANFLRQAGWQRGLAWGYE
VGLPAGFDSSGAGRKNKRGIAEWAGRGVRRADGGALAQAGNAALLLPAGASGPAFLVTRNFDAIYSYNAAESYALAIAHL
SDRLRGAGPFATPWPTDDPGLSRAERREVQTLLLARGHDIGNPDGMIGARTREALRVVQAEFGLPADGRAGRRVLDALRQ
ARP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=245, Percent_Identity=34.6938775510204, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43064; Mature: 42933

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRSKSRFPVSALLAVAPFALSLAAPAAAQDLACLAQLRAPAQAYGVSAATFDRLTAG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCC
LQADPTVLPLLDAQPEFVTPIWDYMAALVDDERIADGRAMLEQWSAVLARVEAEYGVDAA
CCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TVVAVWGVESNYGRNFGSRALLPSLATLACNGRRQSFFRQELFATMKIVEDGHVAPERLN
HEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCC
GSWAGAFGHTQFMPSTYLRLAVDFDGDGRRDLVDSIADALASTANFLRQAGWQRGLAWGY
CCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
EVGLPAGFDSSGAGRKNKRGIAEWAGRGVRRADGGALAQAGNAALLLPAGASGPAFLVTR
ECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCEEEEEECCCCCCEEEEEE
NFDAIYSYNAAESYALAIAHLSDRLRGAGPFATPWPTDDPGLSRAERREVQTLLLARGHD
CCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
IGNPDGMIGARTREALRVVQAEFGLPADGRAGRRVLDALRQARP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTPRSKSRFPVSALLAVAPFALSLAAPAAAQDLACLAQLRAPAQAYGVSAATFDRLTAG
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCC
LQADPTVLPLLDAQPEFVTPIWDYMAALVDDERIADGRAMLEQWSAVLARVEAEYGVDAA
CCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TVVAVWGVESNYGRNFGSRALLPSLATLACNGRRQSFFRQELFATMKIVEDGHVAPERLN
HEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCC
GSWAGAFGHTQFMPSTYLRLAVDFDGDGRRDLVDSIADALASTANFLRQAGWQRGLAWGY
CCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
EVGLPAGFDSSGAGRKNKRGIAEWAGRGVRRADGGALAQAGNAALLLPAGASGPAFLVTR
ECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCEEEEEECCCCCCEEEEEE
NFDAIYSYNAAESYALAIAHLSDRLRGAGPFATPWPTDDPGLSRAERREVQTLLLARGHD
CCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
IGNPDGMIGARTREALRVVQAEFGLPADGRAGRRVLDALRQARP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]