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The map label for this gene is rluE [H]

Identifier: 119897392

GI number: 119897392

Start: 1185090

End: 1185695

Strand: Direct

Name: rluE [H]

Synonym: azo1101

Alternate gene names: 119897392

Gene position: 1185090-1185695 (Clockwise)

Preceding gene: 119897391

Following gene: 119897393

Centisome position: 27.08

GC content: 70.96

Gene sequence:

>606_bases
ATGTCCCGCCTCATCCTCCTGAACAAGCCCTACGGCGTCCTCTGTCAGTTCAGCGGAGAACCGGGTCGCGCCACGCTGAA
GGACCATGTGCCGATCCCCGAGGTCTATCCGGCCGGGCGGCTGGATACCGACAGCGAAGGCTTGTTGTTGCTCACCGACG
ACGGTGCGCTGCAGGCCCGTATCGCCGACCCTCGCCACAAGCTGCCCAAGACCTATCTGGTGCAGGTGGAGGGCGAGCCG
GACGACGCCGCACTGGCGGCCTTGCGCGCCGGCGTGGATCTGGGCGACTTCCGCACCCGCCCCTGCAGTGCGCGCCGCGT
TGTCGAGCCCGACTGGCTGTGGCCGCGCGATCCGCCGGTGCGTTACCGCAAGTCGGTGCCGACATCCTGGGTCGAGATCG
TGCTGCGCGAAGGCAAGAATCGCCAGGTCCGGCGCATGACCGCGAAGGTCGGCTTTCCCACCTTGCGCCTGCTGCGCGTG
GCGATCGGCCCGTGGCGGCTCGACGGGCTGGCGCCGGGGGCCTGGCGCGAAGTCGAACCACGCTTGCCGCCGGCGCCGGA
GGCGCGGTCGGCGGGTGCCCGTCCGGGCGGTCTGCGCAGGCGCTAG

Upstream 100 bases:

>100_bases
TTGAAAACCAGCGTGCATGTGAGTGCACCGTGGGTTCGAATCCCACCCCATCCGCCACTGCGGCCGAAGCCCTCGTCCGT
TCCCCCGCGTGCTCATCCCG

Downstream 100 bases:

>100_bases
AATCGGGCACTTTCCACTTCGTCACCTTACCGCCATGTCCGTCCGTGCCGTGCTGCTGCGCGCCGTTTTCGCCCTCGCTC
TTGGAGCGGCCTCTGCCGCG

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSRLILLNKPYGVLCQFSGEPGRATLKDHVPIPEVYPAGRLDTDSEGLLLLTDDGALQARIADPRHKLPKTYLVQVEGEP
DDAALAALRAGVDLGDFRTRPCSARRVVEPDWLWPRDPPVRYRKSVPTSWVEIVLREGKNRQVRRMTAKVGFPTLRLLRV
AIGPWRLDGLAPGAWREVEPRLPPAPEARSAGARPGGLRRR

Sequences:

>Translated_201_residues
MSRLILLNKPYGVLCQFSGEPGRATLKDHVPIPEVYPAGRLDTDSEGLLLLTDDGALQARIADPRHKLPKTYLVQVEGEP
DDAALAALRAGVDLGDFRTRPCSARRVVEPDWLWPRDPPVRYRKSVPTSWVEIVLREGKNRQVRRMTAKVGFPTLRLLRV
AIGPWRLDGLAPGAWREVEPRLPPAPEARSAGARPGGLRRR
>Mature_200_residues
SRLILLNKPYGVLCQFSGEPGRATLKDHVPIPEVYPAGRLDTDSEGLLLLTDDGALQARIADPRHKLPKTYLVQVEGEPD
DAALAALRAGVDLGDFRTRPCSARRVVEPDWLWPRDPPVRYRKSVPTSWVEIVLREGKNRQVRRMTAKVGFPTLRLLRVA
IGPWRLDGLAPGAWREVEPRLPPAPEARSAGARPGGLRRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=62.7118644067797, Blast_Score=222, Evalue=1e-59,
Organism=Escherichia coli, GI1788510, Length=180, Percent_Identity=35, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1787524, Length=187, Percent_Identity=33.6898395721925, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790453, Length=178, Percent_Identity=32.0224719101124, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 22414; Mature: 22283

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLILLNKPYGVLCQFSGEPGRATLKDHVPIPEVYPAGRLDTDSEGLLLLTDDGALQAR
CCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
IADPRHKLPKTYLVQVEGEPDDAALAALRAGVDLGDFRTRPCSARRVVEPDWLWPRDPPV
ECCCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RYRKSVPTSWVEIVLREGKNRQVRRMTAKVGFPTLRLLRVAIGPWRLDGLAPGAWREVEP
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCCCCC
RLPPAPEARSAGARPGGLRRR
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLILLNKPYGVLCQFSGEPGRATLKDHVPIPEVYPAGRLDTDSEGLLLLTDDGALQAR
CCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
IADPRHKLPKTYLVQVEGEPDDAALAALRAGVDLGDFRTRPCSARRVVEPDWLWPRDPPV
ECCCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RYRKSVPTSWVEIVLREGKNRQVRRMTAKVGFPTLRLLRVAIGPWRLDGLAPGAWREVEP
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCCCCC
RLPPAPEARSAGARPGGLRRR
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]