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The map label for this gene is rumA

Identifier: 119897391

GI number: 119897391

Start: 1183539

End: 1184840

Strand: Direct

Name: rumA

Synonym: azo1100

Alternate gene names: 119897391

Gene position: 1183539-1184840 (Clockwise)

Preceding gene: 119897388

Following gene: 119897392

Centisome position: 27.05

GC content: 67.43

Gene sequence:

>1302_bases
ATGCCCACAGCAGTCATCGAATCGCTGGATCACGAAGGTCGCGGCATCGCTCGTGTGGAGGGAAAGGCGGTCTTCATCGA
GGGCGGTCTGCCCGGCGAAACCGTGGAATACCGCGTCCTGCGCAGCAAACCCAATTACGAACAGGCCGAGGCGACCCGCA
TCGTGCGCCGCAGCGCGCTGCGCGTCGCGCCCCGCTGTCCTCATTTCGGCGTCTGTGGCGGCTGCTCGATGCAGCATCTG
GATACGCTTGCGCAGGCTGCAACCAAGCAACGGGTACTCGAAGACGCGCTGTGGCGCATCGGCAAGGTCAAGCCCGGGAT
CATCTATTCGGCCATCCAGGGGCCGGCCTGGGGCTACCGCTATCGGGCCCGGCTCGGGGTTCGGCTGGTACCGGCCAAGG
GCGGTGTGTTGATCGGCTTCCACGAACGGCGGTCCAGCTACATTGCCGACCTCGGCGTATGCCCGGTGTTGCCGGCGCAC
GTGTCGGCCTTGCTGCCCGCGCTGAAAGTGCTGGTGGCAAGCTTGTCGATCGCCGACCGCCTGCCGCAGATCGAGGTCGC
GGTGAGCGAGGCGACCACGGTGCTGGTGTTTCGAAACCTGCTGGCGCTGAAGAAGGCCGACGAGGCGCAACTGCGCAGTT
TCGCCGAGACCCACGGCGTGCAGGTCTGGCTGCAGCCGGAGGGGCCGGATTCGATCGTGCCGCTGCACCCGCGGTCCGGG
CCTGGACTGACCTACACGCTGCCCGAATTCGACGTCGCCCTCGACTTCCGCGCCACCGACTTCACCCAAGTGAATGTCCA
TATCAACCGCCTGTTGATCCGACGCGCGATGCAGTTGCTGCAGCCGGCGCCCGGTGAGCGGATTGCCGACCTGTTCTGCG
GCCTCGGCAACTTCAGCCTGCCGATCGCGCGCCGCGGCGCGACCGTGGTCGGCGTCGAGGGCAGCGAGGCCCTGGTGGCG
CGCGCGCTGGAGAACGCGCGCCGCAACGGCCTCGATTCGCGCACCGAGTTCCATGCCGCCAACCTGTTCGACGCGACCGA
GGACAGCCTGGCCGCGCTCGGCCCGCTCGACAAGCTGCTGATCGATCCGCCGCGCGAAGGCGCGATCGCGGTGGTGAAGG
CGGTTGGTCCGGCTCAGCAGCCGGCCCGCATCGTCTATGTTTCCTGCAATCCGGCCACGCTCGCGCGCGATGCGGCGGTG
CTGGTCCATGAGAAGGGCTACGTGCTGCGCGGTGCAGGCATCGCCAACATGTTTCCGCAGACCTCCCACGTCGAGTCGAT
TGCGCTGTTCGAACGCCCCTGA

Upstream 100 bases:

>100_bases
GGTGATCATCGGCCGGATTATAAGGGGGTGGCACGTGCGTGGGCGCGCGCCTTGCGGCCGGTTAGCGGTAAACTCGCGTG
CTTTTGCGGCGGGATGTCGG

Downstream 100 bases:

>100_bases
GCAGGGCGGCGAGGGCGGCGCCGATGCTATACTCCGCCGCTTCCGGCAGCTGCGGCGCCGCGCCGCAGCGGGGGCCCTGG
CCGGAGCGCCAGACCCTGCG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MPTAVIESLDHEGRGIARVEGKAVFIEGGLPGETVEYRVLRSKPNYEQAEATRIVRRSALRVAPRCPHFGVCGGCSMQHL
DTLAQAATKQRVLEDALWRIGKVKPGIIYSAIQGPAWGYRYRARLGVRLVPAKGGVLIGFHERRSSYIADLGVCPVLPAH
VSALLPALKVLVASLSIADRLPQIEVAVSEATTVLVFRNLLALKKADEAQLRSFAETHGVQVWLQPEGPDSIVPLHPRSG
PGLTYTLPEFDVALDFRATDFTQVNVHINRLLIRRAMQLLQPAPGERIADLFCGLGNFSLPIARRGATVVGVEGSEALVA
RALENARRNGLDSRTEFHAANLFDATEDSLAALGPLDKLLIDPPREGAIAVVKAVGPAQQPARIVYVSCNPATLARDAAV
LVHEKGYVLRGAGIANMFPQTSHVESIALFERP

Sequences:

>Translated_433_residues
MPTAVIESLDHEGRGIARVEGKAVFIEGGLPGETVEYRVLRSKPNYEQAEATRIVRRSALRVAPRCPHFGVCGGCSMQHL
DTLAQAATKQRVLEDALWRIGKVKPGIIYSAIQGPAWGYRYRARLGVRLVPAKGGVLIGFHERRSSYIADLGVCPVLPAH
VSALLPALKVLVASLSIADRLPQIEVAVSEATTVLVFRNLLALKKADEAQLRSFAETHGVQVWLQPEGPDSIVPLHPRSG
PGLTYTLPEFDVALDFRATDFTQVNVHINRLLIRRAMQLLQPAPGERIADLFCGLGNFSLPIARRGATVVGVEGSEALVA
RALENARRNGLDSRTEFHAANLFDATEDSLAALGPLDKLLIDPPREGAIAVVKAVGPAQQPARIVYVSCNPATLARDAAV
LVHEKGYVLRGAGIANMFPQTSHVESIALFERP
>Mature_432_residues
PTAVIESLDHEGRGIARVEGKAVFIEGGLPGETVEYRVLRSKPNYEQAEATRIVRRSALRVAPRCPHFGVCGGCSMQHLD
TLAQAATKQRVLEDALWRIGKVKPGIIYSAIQGPAWGYRYRARLGVRLVPAKGGVLIGFHERRSSYIADLGVCPVLPAHV
SALLPALKVLVASLSIADRLPQIEVAVSEATTVLVFRNLLALKKADEAQLRSFAETHGVQVWLQPEGPDSIVPLHPRSGP
GLTYTLPEFDVALDFRATDFTQVNVHINRLLIRRAMQLLQPAPGERIADLFCGLGNFSLPIARRGATVVGVEGSEALVAR
ALENARRNGLDSRTEFHAANLFDATEDSLAALGPLDKLLIDPPREGAIAVVKAVGPAQQPARIVYVSCNPATLARDAAVL
VHEKGYVLRGAGIANMFPQTSHVESIALFERP

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=31.7948717948718, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=31.7948717948718, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1789148, Length=433, Percent_Identity=39.7228637413395, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787083, Length=345, Percent_Identity=27.536231884058, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790403, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24666579, Length=200, Percent_Identity=31.5, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_AZOSB (A1K4G2)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932604.1
- ProteinModelPortal:   A1K4G2
- STRING:   A1K4G2
- GeneID:   4606690
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1100
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- PhylomeDB:   A1K4G2
- ProtClustDB:   PRK13168
- BioCyc:   ASP62928:AZO1100-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 46821; Mature: 46689

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 389-389 BINDING 263-263 BINDING 292-292 BINDING 297-297 BINDING 313-313 BINDING 341-341 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTAVIESLDHEGRGIARVEGKAVFIEGGLPGETVEYRVLRSKPNYEQAEATRIVRRSAL
CCCHHHHHCCCCCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
RVAPRCPHFGVCGGCSMQHLDTLAQAATKQRVLEDALWRIGKVKPGIIYSAIQGPAWGYR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
YRARLGVRLVPAKGGVLIGFHERRSSYIADLGVCPVLPAHVSALLPALKVLVASLSIADR
EEEEECEEEEECCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LPQIEVAVSEATTVLVFRNLLALKKADEAQLRSFAETHGVQVWLQPEGPDSIVPLHPRSG
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCC
PGLTYTLPEFDVALDFRATDFTQVNVHINRLLIRRAMQLLQPAPGERIADLFCGLGNFSL
CCCEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
PIARRGATVVGVEGSEALVARALENARRNGLDSRTEFHAANLFDATEDSLAALGPLDKLL
CEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHHH
IDPPREGAIAVVKAVGPAQQPARIVYVSCNPATLARDAAVLVHEKGYVLRGAGIANMFPQ
CCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHCCEEEEEECCCEEEECCCHHHCCCC
TSHVESIALFERP
CCCCCEEEEECCC
>Mature Secondary Structure 
PTAVIESLDHEGRGIARVEGKAVFIEGGLPGETVEYRVLRSKPNYEQAEATRIVRRSAL
CCHHHHHCCCCCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
RVAPRCPHFGVCGGCSMQHLDTLAQAATKQRVLEDALWRIGKVKPGIIYSAIQGPAWGYR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
YRARLGVRLVPAKGGVLIGFHERRSSYIADLGVCPVLPAHVSALLPALKVLVASLSIADR
EEEEECEEEEECCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LPQIEVAVSEATTVLVFRNLLALKKADEAQLRSFAETHGVQVWLQPEGPDSIVPLHPRSG
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCC
PGLTYTLPEFDVALDFRATDFTQVNVHINRLLIRRAMQLLQPAPGERIADLFCGLGNFSL
CCCEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
PIARRGATVVGVEGSEALVARALENARRNGLDSRTEFHAANLFDATEDSLAALGPLDKLL
CEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHHH
IDPPREGAIAVVKAVGPAQQPARIVYVSCNPATLARDAAVLVHEKGYVLRGAGIANMFPQ
CCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHCCEEEEEECCCEEEECCCHHHCCCC
TSHVESIALFERP
CCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA