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The map label for this gene is pcm2

Identifier: 119897379

GI number: 119897379

Start: 1171112

End: 1171717

Strand: Direct

Name: pcm2

Synonym: azo1088

Alternate gene names: 119897379

Gene position: 1171112-1171717 (Clockwise)

Preceding gene: 119897378

Following gene: 119897380

Centisome position: 26.76

GC content: 64.03

Gene sequence:

>606_bases
ATGGTGGAGCGCTTGCGCGCGCAGGGCATTCAGGACGAGGGGGCCCTGGCGGCGATGATGCAGGTGCCGCGACACCTGTT
CGTCGAGGAAGGCTTGGCGTACAGCGCTTACGACGATACGGCACTTCCGATCGGATTCCAGCAAACCATCTCCCAGCCTT
ACGTAGTGGCGAGAATGATCGAATTGCTGCGCAGCGGCGGGCGGCAACTCGGGCGTGTGCTGGAAATCGGGGCGGGGTGC
GGCTATCAGGCCGCGGTGCTGTCCACGCTTGCTACCGAAGTCTATGCAGTGGAGCGTATCCGGCCGCTGCTGGACAAGGC
GCGTGCAAATCTCCGGCCCTTGCGCCTGCCCAACGTCCGCCTCAAACATGCGGACGGAACGCTGGGTTTGCCGGAGGCGG
CGCCCTTTGAAAGCATCATCGTCGCGGCGGCCGCGGGCGGTGTGCCGAATGCCCTCAAGGAACAACTTGCCCCGGGCGGT
CGTCTGATCATCCCGGTCGGGGGTGGTGAACAGCGACTGTTGCTGATCGAACGCCAAGGCAACGTATTCAGGGAGAGCGG
TTACGAGGCGGTGCGCTTCGTGCCGTTGCTTGCAGGTACCGAATGA

Upstream 100 bases:

>100_bases
TCGATTTGACCCACACGGGTCAGATCGCCGCGGTGGATACGTGGTTGTCGGCATGAGCGGCCGCCAGGACGCCTCCCATG
CGGGGCGGGCGCGCGCCCGA

Downstream 100 bases:

>100_bases
ACGACTCAGTGAATATCAAAAAGCGGATTGCTCCTTATTTTCTTGCGCTCGGCGTGCTGTTCCTGGCGGGCTGCGCGTCT
CGCGCTCCGGTTGCGGTGCG

Product: protein-L-isoaspartate (D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MVERLRAQGIQDEGALAAMMQVPRHLFVEEGLAYSAYDDTALPIGFQQTISQPYVVARMIELLRSGGRQLGRVLEIGAGC
GYQAAVLSTLATEVYAVERIRPLLDKARANLRPLRLPNVRLKHADGTLGLPEAAPFESIIVAAAAGGVPNALKEQLAPGG
RLIIPVGGGEQRLLLIERQGNVFRESGYEAVRFVPLLAGTE

Sequences:

>Translated_201_residues
MVERLRAQGIQDEGALAAMMQVPRHLFVEEGLAYSAYDDTALPIGFQQTISQPYVVARMIELLRSGGRQLGRVLEIGAGC
GYQAAVLSTLATEVYAVERIRPLLDKARANLRPLRLPNVRLKHADGTLGLPEAAPFESIIVAAAAGGVPNALKEQLAPGG
RLIIPVGGGEQRLLLIERQGNVFRESGYEAVRFVPLLAGTE
>Mature_201_residues
MVERLRAQGIQDEGALAAMMQVPRHLFVEEGLAYSAYDDTALPIGFQQTISQPYVVARMIELLRSGGRQLGRVLEIGAGC
GYQAAVLSTLATEVYAVERIRPLLDKARANLRPLRLPNVRLKHADGTLGLPEAAPFESIIVAAAAGGVPNALKEQLAPGG
RLIIPVGGGEQRLLLIERQGNVFRESGYEAVRFVPLLAGTE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=208, Percent_Identity=33.6538461538462, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI1789100, Length=199, Percent_Identity=50.7537688442211, Blast_Score=177, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=197, Percent_Identity=30.9644670050761, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=197, Percent_Identity=29.9492385786802, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17981723, Length=213, Percent_Identity=31.4553990610329, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_AZOSB (A1K4F0)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932592.1
- ProteinModelPortal:   A1K4F0
- STRING:   A1K4F0
- GeneID:   4609911
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1088
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- ProtClustDB:   CLSK766294
- BioCyc:   ASP62928:AZO1088-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 21622; Mature: 21622

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERLRAQGIQDEGALAAMMQVPRHLFVEEGLAYSAYDDTALPIGFQQTISQPYVVARMI
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCHHHHHHHH
ELLRSGGRQLGRVLEIGAGCGYQAAVLSTLATEVYAVERIRPLLDKARANLRPLRLPNVR
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LKHADGTLGLPEAAPFESIIVAAAAGGVPNALKEQLAPGGRLIIPVGGGEQRLLLIERQG
EEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEEECCC
NVFRESGYEAVRFVPLLAGTE
CCHHHCCCCEEEEEEECCCCC
>Mature Secondary Structure
MVERLRAQGIQDEGALAAMMQVPRHLFVEEGLAYSAYDDTALPIGFQQTISQPYVVARMI
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCHHHHHHHH
ELLRSGGRQLGRVLEIGAGCGYQAAVLSTLATEVYAVERIRPLLDKARANLRPLRLPNVR
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LKHADGTLGLPEAAPFESIIVAAAAGGVPNALKEQLAPGGRLIIPVGGGEQRLLLIERQG
EEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEEECCC
NVFRESGYEAVRFVPLLAGTE
CCHHHCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA