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The map label for this gene is ispB [H]

Identifier: 119897305

GI number: 119897305

Start: 1090402

End: 1091427

Strand: Direct

Name: ispB [H]

Synonym: azo1014

Alternate gene names: 119897305

Gene position: 1090402-1091427 (Clockwise)

Preceding gene: 119897304

Following gene: 119897306

Centisome position: 24.92

GC content: 63.65

Gene sequence:

>1026_bases
TTGACGCGCGTTGTCGGGCACCCCTACCATCCCGGTTTTTCCACGAGCACCGCCGTCTTGTCCGTCCAGCAGCTATTCGC
GCCGATCGCAGCCGACATGCAGGCGGTCGATGCGCTCATCCGCAAGGCCCTCTATTCCGACGTGGTGCTGATCCGTCAGG
TGGCGGAGTACATCATCAACAGCGGCGGAAAGCGCCTGCGCCCCGCGCTGGTGCTGTTCAGCGCCGGCGCGGTCGGCTAC
CGGGGCACACAGCATCATCGTCTCGCCGCGGTGGTCGAATTCATCCACACCTCGACGCTGCTGCACGACGACGTGGTCGA
CGAATCGGAACTGAGGCGCGGCAACAAGACGGCCAATGCGCTGTTCGGCAATGCCGCTTCGGTTCTCGTGGGCGACTTCC
TGTATACCCGCGCCTTCCAGATGATGGTCGAGGTCGACAGCATGCGGGTGATGCAGATCCTTGCCGACGCGACGAACATC
ATTGCAGAAGGCGAAGTGCTGCAGCTGTTGAACTGCCATAACGCCGACGTCGAGGTGGAGGACTACCTCCGCGTGATCCG
CTACAAGACGGCCAAGCTGTTCGAAGCGGCCTCGCGCCTTGGGGCCGTCCTCGGTGGCGCCGATCCGGCGCTCGAGGAGA
GCATGGCCGCCTTCGGCATGCACCTCGGCACCGCCTTCCAGCTCATCGACGATGTGCTGGATTACTCGGCTGATGAAGCC
GACACCGGCAAGCACCTGGGTGATGATCTGGCCGAGGGCAAGCCGACCTTGCCGCTGATCCACGTCATGCAGCACGGCAG
CGCGGAGCAGGCAGCCCTGGTGCGCGGCGCAATCGAGAACGGCGGCCGCGACGATTTCGCCGCGGTGCTCGCAGCGATCC
AGCAGACGGGGGCGCTCGAGGTGACGCGCCGCCACGCGCGCGAAGAGGCCGATCGCGCAATTGCAGCATTGCAAGGATTG
CCCCCTTCCAATTTCAAGGAATCGCTGCTACAATTATCGGACTTTGCAGTTGAGAGAAATCACTGA

Upstream 100 bases:

>100_bases
CGCGGCACTTCGCCGTACGCCCTGCCGACACACCGGGCGGGCCTGCGCCGGCACCTTCACAATCTTACTTTCGGAGTAGG
GCTTCCGCCCGGAGGCCGTG

Downstream 100 bases:

>100_bases
TTTCAATCGGCAGCAAATCCAGTCGGGGAATAGCTCAGCCTGGTAGAGCACTGCGTTCGGGACGCAGGGGCCGGAGGTTC
GAATCCTCTTTCCCCGACCA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTRVVGHPYHPGFSTSTAVLSVQQLFAPIAADMQAVDALIRKALYSDVVLIRQVAEYIINSGGKRLRPALVLFSAGAVGY
RGTQHHRLAAVVEFIHTSTLLHDDVVDESELRRGNKTANALFGNAASVLVGDFLYTRAFQMMVEVDSMRVMQILADATNI
IAEGEVLQLLNCHNADVEVEDYLRVIRYKTAKLFEAASRLGAVLGGADPALEESMAAFGMHLGTAFQLIDDVLDYSADEA
DTGKHLGDDLAEGKPTLPLIHVMQHGSAEQAALVRGAIENGGRDDFAAVLAAIQQTGALEVTRRHAREEADRAIAALQGL
PPSNFKESLLQLSDFAVERNH

Sequences:

>Translated_341_residues
MTRVVGHPYHPGFSTSTAVLSVQQLFAPIAADMQAVDALIRKALYSDVVLIRQVAEYIINSGGKRLRPALVLFSAGAVGY
RGTQHHRLAAVVEFIHTSTLLHDDVVDESELRRGNKTANALFGNAASVLVGDFLYTRAFQMMVEVDSMRVMQILADATNI
IAEGEVLQLLNCHNADVEVEDYLRVIRYKTAKLFEAASRLGAVLGGADPALEESMAAFGMHLGTAFQLIDDVLDYSADEA
DTGKHLGDDLAEGKPTLPLIHVMQHGSAEQAALVRGAIENGGRDDFAAVLAAIQQTGALEVTRRHAREEADRAIAALQGL
PPSNFKESLLQLSDFAVERNH
>Mature_340_residues
TRVVGHPYHPGFSTSTAVLSVQQLFAPIAADMQAVDALIRKALYSDVVLIRQVAEYIINSGGKRLRPALVLFSAGAVGYR
GTQHHRLAAVVEFIHTSTLLHDDVVDESELRRGNKTANALFGNAASVLVGDFLYTRAFQMMVEVDSMRVMQILADATNII
AEGEVLQLLNCHNADVEVEDYLRVIRYKTAKLFEAASRLGAVLGGADPALEESMAAFGMHLGTAFQLIDDVLDYSADEAD
TGKHLGDDLAEGKPTLPLIHVMQHGSAEQAALVRGAIENGGRDDFAAVLAAIQQTGALEVTRRHAREEADRAIAALQGLP
PSNFKESLLQLSDFAVERNH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=316, Percent_Identity=30.0632911392405, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI4758430, Length=272, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI83700220, Length=272, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=54.6583850931677, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786623, Length=336, Percent_Identity=31.25, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17505681, Length=312, Percent_Identity=28.2051282051282, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=29.5681063122924, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=236, Percent_Identity=26.271186440678, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=28.9115646258503, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365769, Length=261, Percent_Identity=24.5210727969349, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=261, Percent_Identity=24.5210727969349, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36895; Mature: 36764

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVVGHPYHPGFSTSTAVLSVQQLFAPIAADMQAVDALIRKALYSDVVLIRQVAEYIIN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGGKRLRPALVLFSAGAVGYRGTQHHRLAAVVEFIHTSTLLHDDVVDESELRRGNKTANA
CCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHH
LFGNAASVLVGDFLYTRAFQMMVEVDSMRVMQILADATNIIAEGEVLQLLNCHNADVEVE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHH
DYLRVIRYKTAKLFEAASRLGAVLGGADPALEESMAAFGMHLGTAFQLIDDVLDYSADEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTGKHLGDDLAEGKPTLPLIHVMQHGSAEQAALVRGAIENGGRDDFAAVLAAIQQTGALE
CCCHHHCHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
VTRRHAREEADRAIAALQGLPPSNFKESLLQLSDFAVERNH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRVVGHPYHPGFSTSTAVLSVQQLFAPIAADMQAVDALIRKALYSDVVLIRQVAEYIIN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGGKRLRPALVLFSAGAVGYRGTQHHRLAAVVEFIHTSTLLHDDVVDESELRRGNKTANA
CCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHH
LFGNAASVLVGDFLYTRAFQMMVEVDSMRVMQILADATNIIAEGEVLQLLNCHNADVEVE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHH
DYLRVIRYKTAKLFEAASRLGAVLGGADPALEESMAAFGMHLGTAFQLIDDVLDYSADEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTGKHLGDDLAEGKPTLPLIHVMQHGSAEQAALVRGAIENGGRDDFAAVLAAIQQTGALE
CCCHHHCHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
VTRRHAREEADRAIAALQGLPPSNFKESLLQLSDFAVERNH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]