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The map label for this gene is nasC [H]

Identifier: 119897237

GI number: 119897237

Start: 1015380

End: 1016627

Strand: Direct

Name: nasC [H]

Synonym: azo0946

Alternate gene names: 119897237

Gene position: 1015380-1016627 (Clockwise)

Preceding gene: 119897236

Following gene: 119897238

Centisome position: 23.2

GC content: 69.15

Gene sequence:

>1248_bases
ATGCGCAAACAGAAACTCGTCATGGTCGGCAACGGCATGGCCGGCGTGAAGACGCTGGAAGAGCTGCTCAAGCACGCGCC
GGATGCGTTCGAGATCACCGTGTTCGGCGCCGAGCCGCACGGCAATTACAACCGCATCCTGCTGTCGCCGGTGCTGGCGG
GCGAAATGAGCCTGCCGGAGATCATGCTCAACGACATCGGCTGGTACCGCGCCCACGGCATCACGCTGCATGCAGGCCGC
CGCGTGGTGGAGATCGACCGCGCCCGCCGCATCGTGCGCGCCGACGACGGCACCGAGGCCGCCTACGACCGCCTGCTGCT
CGCCACCGGCTCGGCGCCTTTCATCCTGCCGGTGCCCGGCCGCGAACTGCCCGGGGTTATCGCCTACCGCGACATCGCCG
ACACCGAGGCAATGATCGACGCGGCCCGCCAGCACCGCCACGCGGTGGTGATCGGCGCCGGCCTGCTTGGCCTCGAAGCG
GCGAATGGACTGGTGCTGCGCGGCATGGACGTCACCGTGGTGCATCTGGGCGGCTGGATCATGGATCGCCAGCTCGACCA
GCCGGCGGCAGAGCTGCTGCAGGCTTCGCTCGAAGCCAAGGGCATGCGCTTCCGCCTGCAGGCACAGACCGCGGCGCTGC
TGCCGGGCACGGGCGGGCGCGTTGCTGCCGTGCAGTTGCAGGACGGCAGCACGCTCCCGGCCGATCTGGTCGTGATGGCC
GCCGGCATCCGCCCCAATACCGCGCTGGCCGAAGCGGCCAAGCTGCGCTGCGAGCGCGGCATCGTCGTCCACGACACGCT
GCAGACAGTGACCGATCCGCGCATCTACGCGGTGGGTGAATGCGTCAATCATCGCGGCACCGCTTACGGCCTGGTGGCGC
CGCTGTTCGAGCAGGCCCGCGTCTGCGCCAACCATCTCGCCGGCCACGGCATCGGCCGCTACACCGGCTCGGTAACCTCG
ACCAAGCTCAAGGTTACCGGCGTCGATGTGTTCTCCGCCGGCGACTTCATGGGCGGCGAAGGCTGCGACGAGATCGTGCT
GCACGACCGTGCCGGCGGCATCTACAAGAAGCTGGTGCTCAAGGAGAACCGGCTTGCCGGCGCGGTGATGGTGGGCGATA
CCGCCGACGGCGCCTGGTACTTCCAGCTGGTGCGCGAGGGCGGCGACGTTTCGGCACTGCGCGACCACATGATGTTCGGC
CAGCGCTTCACGCAGTCCCAGCTGGCGGGCGCCGCCTGCCCGGGCTGA

Upstream 100 bases:

>100_bases
TCATCGTCGTCGGCGAGGTCGCCGCGCTTGCCCGAGTGCCCGGACTGGCGATGGATGAGTCCGACGTGCGCGCGCTCGCC
GCGTGAGCCAGGAGAGCGCA

Downstream 100 bases:

>100_bases
GCGCGCGGCGACGGCGAGACGCAAAGGAATGAAGACATGGAGCCCCACATGAACCTGGCCGACCTGGGCCTGAAGGCCAA
CGAAACCCGCTCGGTGTGCT

Product: putative nitrate reductase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRKQKLVMVGNGMAGVKTLEELLKHAPDAFEITVFGAEPHGNYNRILLSPVLAGEMSLPEIMLNDIGWYRAHGITLHAGR
RVVEIDRARRIVRADDGTEAAYDRLLLATGSAPFILPVPGRELPGVIAYRDIADTEAMIDAARQHRHAVVIGAGLLGLEA
ANGLVLRGMDVTVVHLGGWIMDRQLDQPAAELLQASLEAKGMRFRLQAQTAALLPGTGGRVAAVQLQDGSTLPADLVVMA
AGIRPNTALAEAAKLRCERGIVVHDTLQTVTDPRIYAVGECVNHRGTAYGLVAPLFEQARVCANHLAGHGIGRYTGSVTS
TKLKVTGVDVFSAGDFMGGEGCDEIVLHDRAGGIYKKLVLKENRLAGAVMVGDTADGAWYFQLVREGGDVSALRDHMMFG
QRFTQSQLAGAACPG

Sequences:

>Translated_415_residues
MRKQKLVMVGNGMAGVKTLEELLKHAPDAFEITVFGAEPHGNYNRILLSPVLAGEMSLPEIMLNDIGWYRAHGITLHAGR
RVVEIDRARRIVRADDGTEAAYDRLLLATGSAPFILPVPGRELPGVIAYRDIADTEAMIDAARQHRHAVVIGAGLLGLEA
ANGLVLRGMDVTVVHLGGWIMDRQLDQPAAELLQASLEAKGMRFRLQAQTAALLPGTGGRVAAVQLQDGSTLPADLVVMA
AGIRPNTALAEAAKLRCERGIVVHDTLQTVTDPRIYAVGECVNHRGTAYGLVAPLFEQARVCANHLAGHGIGRYTGSVTS
TKLKVTGVDVFSAGDFMGGEGCDEIVLHDRAGGIYKKLVLKENRLAGAVMVGDTADGAWYFQLVREGGDVSALRDHMMFG
QRFTQSQLAGAACPG
>Mature_415_residues
MRKQKLVMVGNGMAGVKTLEELLKHAPDAFEITVFGAEPHGNYNRILLSPVLAGEMSLPEIMLNDIGWYRAHGITLHAGR
RVVEIDRARRIVRADDGTEAAYDRLLLATGSAPFILPVPGRELPGVIAYRDIADTEAMIDAARQHRHAVVIGAGLLGLEA
ANGLVLRGMDVTVVHLGGWIMDRQLDQPAAELLQASLEAKGMRFRLQAQTAALLPGTGGRVAAVQLQDGSTLPADLVVMA
AGIRPNTALAEAAKLRCERGIVVHDTLQTVTDPRIYAVGECVNHRGTAYGLVAPLFEQARVCANHLAGHGIGRYTGSVTS
TKLKVTGVDVFSAGDFMGGEGCDEIVLHDRAGGIYKKLVLKENRLAGAVMVGDTADGAWYFQLVREGGDVSALRDHMMFG
QRFTQSQLAGAACPG

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=279, Percent_Identity=28.3154121863799, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI65787454, Length=279, Percent_Identity=28.3154121863799, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI226437568, Length=279, Percent_Identity=28.3154121863799, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789765, Length=390, Percent_Identity=37.1794871794872, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789065, Length=308, Percent_Identity=29.5454545454545, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=28.9285714285714, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=397, Percent_Identity=23.4256926952141, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281359715, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI281359713, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639250, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18543267, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639252, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639257, Length=280, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585130, Length=269, Percent_Identity=23.7918215613383, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24581020, Length=212, Percent_Identity=30.188679245283, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573993, Length=212, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358499, Length=237, Percent_Identity=27.4261603375527, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 44322; Mature: 44322

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQKLVMVGNGMAGVKTLEELLKHAPDAFEITVFGAEPHGNYNRILLSPVLAGEMSLPE
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHCCCCCCHH
IMLNDIGWYRAHGITLHAGRRVVEIDRARRIVRADDGTEAAYDRLLLATGSAPFILPVPG
HHHHCCCEEEECCEEEECCCEEEEEHHHHHEEECCCCCHHHHHEEEEEECCCCEEEECCC
RELPGVIAYRDIADTEAMIDAARQHRHAVVIGAGLLGLEAANGLVLRGMDVTVVHLGGWI
CCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCEEEHHCCCCEEEECCCEEEEEECCEE
MDRQLDQPAAELLQASLEAKGMRFRLQAQTAALLPGTGGRVAAVQLQDGSTLPADLVVMA
ECCCCCCHHHHHHHHHHHCCCCEEEEEECEEEECCCCCCEEEEEEECCCCCCCCEEEEEE
AGIRPNTALAEAAKLRCERGIVVHDTLQTVTDPRIYAVGECVNHRGTAYGLVAPLFEQAR
CCCCCCHHHHHHHHHHHHCCEEEEECHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHH
VCANHLAGHGIGRYTGSVTSTKLKVTGVDVFSAGDFMGGEGCDEIVLHDRAGGIYKKLVL
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHE
KENRLAGAVMVGDTADGAWYFQLVREGGDVSALRDHMMFGQRFTQSQLAGAACPG
ECCCCEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRKQKLVMVGNGMAGVKTLEELLKHAPDAFEITVFGAEPHGNYNRILLSPVLAGEMSLPE
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHCCCCCCHH
IMLNDIGWYRAHGITLHAGRRVVEIDRARRIVRADDGTEAAYDRLLLATGSAPFILPVPG
HHHHCCCEEEECCEEEECCCEEEEEHHHHHEEECCCCCHHHHHEEEEEECCCCEEEECCC
RELPGVIAYRDIADTEAMIDAARQHRHAVVIGAGLLGLEAANGLVLRGMDVTVVHLGGWI
CCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCEEEHHCCCCEEEECCCEEEEEECCEE
MDRQLDQPAAELLQASLEAKGMRFRLQAQTAALLPGTGGRVAAVQLQDGSTLPADLVVMA
ECCCCCCHHHHHHHHHHHCCCCEEEEEECEEEECCCCCCEEEEEEECCCCCCCCEEEEEE
AGIRPNTALAEAAKLRCERGIVVHDTLQTVTDPRIYAVGECVNHRGTAYGLVAPLFEQAR
CCCCCCHHHHHHHHHHHHCCEEEEECHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHH
VCANHLAGHGIGRYTGSVTSTKLKVTGVDVFSAGDFMGGEGCDEIVLHDRAGGIYKKLVL
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHE
KENRLAGAVMVGDTADGAWYFQLVREGGDVSALRDHMMFGQRFTQSQLAGAACPG
ECCCCEEEEEECCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]