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The map label for this gene is ispG [H]

Identifier: 119897218

GI number: 119897218

Start: 992856

End: 994103

Strand: Direct

Name: ispG [H]

Synonym: azo0927

Alternate gene names: 119897218

Gene position: 992856-994103 (Clockwise)

Preceding gene: 119897217

Following gene: 119897219

Centisome position: 22.69

GC content: 67.95

Gene sequence:

>1248_bases
ATGGTGGAACAGGCTTTTCCCTTCGGCTCGCTGCCGCGCCGGCCGACGCGGCAGGTGCGCATCGGCGGCGTCTCGGTCGG
CTCCGCTGCGCCGGTCGTCGTGCAGTCCATGACCAACACCGACACCGCCGACGTGCTGGGTACCGCGATGCAGGTCGCGG
AACTCGCGCGCGCCGGTTCGGAACTCGTCCGCATCACGGTGAACAACGAGGCCGCCGCCAAGGCCGTGCCCCACATCCGC
GACCGCTTGCTGGCGTTGAACATGGACGTGCCGCTGGTCGGCGACTTCCACTACAACGGCCACAAGCTGCTGATGGACAA
CCCGGCGTGTGCCGAGGCGCTCGCGAAGCTGCGCATCAATCCCGGCAACGTGGGCGCTGGCGCCAAGCGCGACCCGCAGT
TCGCCGCCATCGTGGAGATGGCCTGCAAGTACGACAAGCCGGTGCGGATCGGAGTGAATTGGGGCAGCCTCGACCAGTCG
GTGCTGGCCCGCATCATGGACGAGAACGCCACCCGGGCAGAGCCGCGCGATGCCGGCGCCGTGATGCGTGAGGCGCTGGT
CGTGTCGGCGCTGGAGTCCGCGGCCAAGGCCGAGGAGTACGGCCTCGCCGGCGACCGCATCATCCTGTCGGCCAAGGTGT
CGAGCGTGCAGGACCTGATCGCCGTCTATCGCGACATGGCGCGCCGCTGCGATTACCCCTTGCATCTCGGGCTGACCGAG
GCGGGCATGGGCAGCAAGGGCATCGTGGCTTCCACCGCGGCGCTCTCGGTGCTGCTTCAGGAAGGCATCGGCGACACGAT
CCGGATCTCGCTGACGCCGGAACCGAACGGCAGCCGCACCCAGGAAGTGGTGGTGGCACAGGAAATCCTGCAGACCATGG
GCCTGCGCGCGTTCACGCCGATGGTGACCGCCTGCCCGGGTTGCGGCCGCACCACCAGCACCTTCTTCCAGGAGCTGGCG
TCCGGCATCCAGGGGTATGTGCGCGAGCAGATGCCGCTGTGGCGCGAGCAGTATGACGGCGTGGAGACCATGACCCTTGC
CGTGATGGGGTGCGTGGTCAACGGCCCGGGCGAAAGCAAGCACGCCAACATCGGCATCTCTCTGCCGGGTACCGGCGAAA
GCCCGGCGGCGCCGGTATTCGTCGATGGCGAGAAGACGGTGACCCTGCGCGGCGACAACATTGCGGCGGAGTTCAAGGCC
ATCGTGGACAACTACGTGGCGACCCGCTACCAGAAGAAGGCGGGCTGA

Upstream 100 bases:

>100_bases
TGGTGGGTAATGCCAAGGATGTGCGGCTGGAATTCAACGGCCGCGAGATTGACCTGCAGCCGCATACGCGCGTCGCGGTT
GCCCGGCTCACGGTGCAATG

Downstream 100 bases:

>100_bases
GGCCCGCGACAACAACACCGACAACGATCACACGATGAGTCAGACGCTGCAGGCCGTGCGCGGGATGAACGACATCCTGC
CCGACGAGGCCGAAACCTGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVEQAFPFGSLPRRPTRQVRIGGVSVGSAAPVVVQSMTNTDTADVLGTAMQVAELARAGSELVRITVNNEAAAKAVPHIR
DRLLALNMDVPLVGDFHYNGHKLLMDNPACAEALAKLRINPGNVGAGAKRDPQFAAIVEMACKYDKPVRIGVNWGSLDQS
VLARIMDENATRAEPRDAGAVMREALVVSALESAAKAEEYGLAGDRIILSAKVSSVQDLIAVYRDMARRCDYPLHLGLTE
AGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPNGSRTQEVVVAQEILQTMGLRAFTPMVTACPGCGRTTSTFFQELA
SGIQGYVREQMPLWREQYDGVETMTLAVMGCVVNGPGESKHANIGISLPGTGESPAAPVFVDGEKTVTLRGDNIAAEFKA
IVDNYVATRYQKKAG

Sequences:

>Translated_415_residues
MVEQAFPFGSLPRRPTRQVRIGGVSVGSAAPVVVQSMTNTDTADVLGTAMQVAELARAGSELVRITVNNEAAAKAVPHIR
DRLLALNMDVPLVGDFHYNGHKLLMDNPACAEALAKLRINPGNVGAGAKRDPQFAAIVEMACKYDKPVRIGVNWGSLDQS
VLARIMDENATRAEPRDAGAVMREALVVSALESAAKAEEYGLAGDRIILSAKVSSVQDLIAVYRDMARRCDYPLHLGLTE
AGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPNGSRTQEVVVAQEILQTMGLRAFTPMVTACPGCGRTTSTFFQELA
SGIQGYVREQMPLWREQYDGVETMTLAVMGCVVNGPGESKHANIGISLPGTGESPAAPVFVDGEKTVTLRGDNIAAEFKA
IVDNYVATRYQKKAG
>Mature_415_residues
MVEQAFPFGSLPRRPTRQVRIGGVSVGSAAPVVVQSMTNTDTADVLGTAMQVAELARAGSELVRITVNNEAAAKAVPHIR
DRLLALNMDVPLVGDFHYNGHKLLMDNPACAEALAKLRINPGNVGAGAKRDPQFAAIVEMACKYDKPVRIGVNWGSLDQS
VLARIMDENATRAEPRDAGAVMREALVVSALESAAKAEEYGLAGDRIILSAKVSSVQDLIAVYRDMARRCDYPLHLGLTE
AGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPNGSRTQEVVVAQEILQTMGLRAFTPMVTACPGCGRTTSTFFQELA
SGIQGYVREQMPLWREQYDGVETMTLAVMGCVVNGPGESKHANIGISLPGTGESPAAPVFVDGEKTVTLRGDNIAAEFKA
IVDNYVATRYQKKAG

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=390, Percent_Identity=33.0769230769231, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44138; Mature: 44138

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQAFPFGSLPRRPTRQVRIGGVSVGSAAPVVVQSMTNTDTADVLGTAMQVAELARAGS
CCCCCCCCCCCCCCCCCEEEECCEECCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHCCC
ELVRITVNNEAAAKAVPHIRDRLLALNMDVPLVGDFHYNGHKLLMDNPACAEALAKLRIN
EEEEEEECCCHHHHHHHHHHHHHEEEECCCCEEEEEEECCEEEEECCHHHHHHHHHHCCC
PGNVGAGAKRDPQFAAIVEMACKYDKPVRIGVNWGSLDQSVLARIMDENATRAEPRDAGA
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VMREALVVSALESAAKAEEYGLAGDRIILSAKVSSVQDLIAVYRDMARRCDYPLHLGLTE
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCC
AGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPNGSRTQEVVVAQEILQTMGLRAFTP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCH
MVTACPGCGRTTSTFFQELASGIQGYVREQMPLWREQYDGVETMTLAVMGCVVNGPGESK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCC
HANIGISLPGTGESPAAPVFVDGEKTVTLRGDNIAAEFKAIVDNYVATRYQKKAG
CCEEEEECCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEQAFPFGSLPRRPTRQVRIGGVSVGSAAPVVVQSMTNTDTADVLGTAMQVAELARAGS
CCCCCCCCCCCCCCCCCEEEECCEECCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHCCC
ELVRITVNNEAAAKAVPHIRDRLLALNMDVPLVGDFHYNGHKLLMDNPACAEALAKLRIN
EEEEEEECCCHHHHHHHHHHHHHEEEECCCCEEEEEEECCEEEEECCHHHHHHHHHHCCC
PGNVGAGAKRDPQFAAIVEMACKYDKPVRIGVNWGSLDQSVLARIMDENATRAEPRDAGA
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VMREALVVSALESAAKAEEYGLAGDRIILSAKVSSVQDLIAVYRDMARRCDYPLHLGLTE
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCC
AGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPNGSRTQEVVVAQEILQTMGLRAFTP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCH
MVTACPGCGRTTSTFFQELASGIQGYVREQMPLWREQYDGVETMTLAVMGCVVNGPGESK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCC
HANIGISLPGTGESPAAPVFVDGEKTVTLRGDNIAAEFKAIVDNYVATRYQKKAG
CCEEEEECCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA