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The map label for this gene is hisS

Identifier: 119897219

GI number: 119897219

Start: 994138

End: 995439

Strand: Direct

Name: hisS

Synonym: azo0928

Alternate gene names: 119897219

Gene position: 994138-995439 (Clockwise)

Preceding gene: 119897218

Following gene: 119897220

Centisome position: 22.72

GC content: 66.74

Gene sequence:

>1302_bases
ATGAGTCAGACGCTGCAGGCCGTGCGCGGGATGAACGACATCCTGCCCGACGAGGCCGAAACCTGGGAACACTTCGAAGA
TATCGTCCGCGACTGGCTGAAGAGCTACGGCTACCGGCCGATCCGCATGCCGCTGGTCGAGCCGACGCCGCTGTTCAAGC
GCGCCATCGGCGAGGTCACCGACATCGTCGAGAAGGAGATGTATTCCTTCGAGGATGCGCTCAACGGCGAGCACCTCACC
TTGCGTCCGGAGGGCACTGCGTCCTGCGTGCGCGCGGCCATCCAGCACAACCTGGTGGCTGGCCACGGGCCGCAGCGGCT
CTACTACCACGGCCCGATGTTCCGCCACGAACGGCCGCAGAAAGGCCGCTACCGCCAGTTCCATCAGATCGGCGTCGAAG
CGCTCGGCTTTGCCGGCCCGGACATCGATGCCGAGCACATCATCATGTGCGCCCGACTGTGGGACGACCTCGGCCTCGAG
GATGTCAGCCTCGAGATCAACTCGCTCGGCGCCGCCGAGGAGCGCGCCCAGCATCGCGCGGCGTTGATCGCCTATCTGGA
ACAGCACCGCGACAAGCTGGACGAGGACGGTCAGCGGCGCCTCTATACCAATCCGTTGCGCATCCTCGACACCAAGAACC
CCGATCTGCAGCCGGTCGTCGAAGCCGCGCCCCGCCTTGCCGATTATCTCGGTGATGAAAGCCGCGCGCATTTCGACGCC
GTGCAGCTGTTCCTCAAGGACGCGGGTATTCCGTATCGCATCAACCACCGCCTGGTGCGGGGGCTGGACTACTACAACCG
CACCGTGTTCGAGTGGGTCACCACACGGCTGGGCGCGCAGGGCACGGTCTGCGCAGGCGGACGCTACGACGGCCTCGTGG
CGCAACTCGGGGGCAAGCCGCAGCCTGCCGCGGGCTTTGCGATGGGGGTGGAGCGCCTGCTGGCGCTGTGGCAGGAAAGT
GGCGGCGAACCCGAGCGCCAGGCGCCCGATGTTTATGTGGTGCATCTCGGCGAGGCGGCTCAACGCCTTGCCTTCCAGGC
GGCTGAAGCGCTGCGCGGCCACGGCTTTTCCGCGGTGCTGCACTGCGGCGGCGGCAGCTTCAAGTCGCAGATGAAGAAGG
CGGACGGTAGTGGCGCCTCGATCGCGGTGGTGATCGGTGAGGACGAAGCGGCCGCCGGCGAAGTGGGTGTGAAGCCGTTG
CGCGGCCCTGGCGGCCAGCAGCGCGTGACTCTTGCTGCCCTGGCCGAAGCGGTGGGCGGCGTGCTGTATTCCGACCAGGG
AGATGACGATGGCGGTGTATGA

Upstream 100 bases:

>100_bases
GCGGCGGAGTTCAAGGCCATCGTGGACAACTACGTGGCGACCCGCTACCAGAAGAAGGCGGGCTGAGGCCCGCGACAACA
ACACCGACAACGATCACACG

Downstream 100 bases:

>100_bases
CCTCGAAGAGCAGGAACAGATCTCCGAGCTGAAGGCGTGGTGGGCGCGGTATGGCAAGCTGGTGACCGCCCTGGCGGTGG
CCGCCGCGGTGGCATCGGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSQTLQAVRGMNDILPDEAETWEHFEDIVRDWLKSYGYRPIRMPLVEPTPLFKRAIGEVTDIVEKEMYSFEDALNGEHLT
LRPEGTASCVRAAIQHNLVAGHGPQRLYYHGPMFRHERPQKGRYRQFHQIGVEALGFAGPDIDAEHIIMCARLWDDLGLE
DVSLEINSLGAAEERAQHRAALIAYLEQHRDKLDEDGQRRLYTNPLRILDTKNPDLQPVVEAAPRLADYLGDESRAHFDA
VQLFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRLGAQGTVCAGGRYDGLVAQLGGKPQPAAGFAMGVERLLALWQES
GGEPERQAPDVYVVHLGEAAQRLAFQAAEALRGHGFSAVLHCGGGSFKSQMKKADGSGASIAVVIGEDEAAAGEVGVKPL
RGPGGQQRVTLAALAEAVGGVLYSDQGDDDGGV

Sequences:

>Translated_433_residues
MSQTLQAVRGMNDILPDEAETWEHFEDIVRDWLKSYGYRPIRMPLVEPTPLFKRAIGEVTDIVEKEMYSFEDALNGEHLT
LRPEGTASCVRAAIQHNLVAGHGPQRLYYHGPMFRHERPQKGRYRQFHQIGVEALGFAGPDIDAEHIIMCARLWDDLGLE
DVSLEINSLGAAEERAQHRAALIAYLEQHRDKLDEDGQRRLYTNPLRILDTKNPDLQPVVEAAPRLADYLGDESRAHFDA
VQLFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRLGAQGTVCAGGRYDGLVAQLGGKPQPAAGFAMGVERLLALWQES
GGEPERQAPDVYVVHLGEAAQRLAFQAAEALRGHGFSAVLHCGGGSFKSQMKKADGSGASIAVVIGEDEAAAGEVGVKPL
RGPGGQQRVTLAALAEAVGGVLYSDQGDDDGGV
>Mature_432_residues
SQTLQAVRGMNDILPDEAETWEHFEDIVRDWLKSYGYRPIRMPLVEPTPLFKRAIGEVTDIVEKEMYSFEDALNGEHLTL
RPEGTASCVRAAIQHNLVAGHGPQRLYYHGPMFRHERPQKGRYRQFHQIGVEALGFAGPDIDAEHIIMCARLWDDLGLED
VSLEINSLGAAEERAQHRAALIAYLEQHRDKLDEDGQRRLYTNPLRILDTKNPDLQPVVEAAPRLADYLGDESRAHFDAV
QLFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRLGAQGTVCAGGRYDGLVAQLGGKPQPAAGFAMGVERLLALWQESG
GEPERQAPDVYVVHLGEAAQRLAFQAAEALRGHGFSAVLHCGGGSFKSQMKKADGSGASIAVVIGEDEAAAGEVGVKPLR
GPGGQQRVTLAALAEAVGGVLYSDQGDDDGGV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=407, Percent_Identity=59.4594594594595, Blast_Score=478, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6325290, Length=461, Percent_Identity=21.6919739696312, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_AZOSB (A1K3Z0)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932432.1
- ProteinModelPortal:   A1K3Z0
- SMR:   A1K3Z0
- STRING:   A1K3Z0
- GeneID:   4608868
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0928
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   A1K3Z0
- ProtClustDB:   PRK00037
- BioCyc:   ASP62928:AZO0928-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47538; Mature: 47407

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLQAVRGMNDILPDEAETWEHFEDIVRDWLKSYGYRPIRMPLVEPTPLFKRAIGEVT
CCHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHH
DIVEKEMYSFEDALNGEHLTLRPEGTASCVRAAIQHNLVAGHGPQRLYYHGPMFRHERPQ
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCC
KGRYRQFHQIGVEALGFAGPDIDAEHIIMCARLWDDLGLEDVSLEINSLGAAEERAQHRA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHHH
ALIAYLEQHRDKLDEDGQRRLYTNPLRILDTKNPDLQPVVEAAPRLADYLGDESRAHFDA
HHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VQLFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRLGAQGTVCAGGRYDGLVAQLGGKP
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHCCCCC
QPAAGFAMGVERLLALWQESGGEPERQAPDVYVVHLGEAAQRLAFQAAEALRGHGFSAVL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEE
HCGGGSFKSQMKKADGSGASIAVVIGEDEAAAGEVGVKPLRGPGGQQRVTLAALAEAVGG
ECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
VLYSDQGDDDGGV
EEECCCCCCCCCC
>Mature Secondary Structure 
SQTLQAVRGMNDILPDEAETWEHFEDIVRDWLKSYGYRPIRMPLVEPTPLFKRAIGEVT
CHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHH
DIVEKEMYSFEDALNGEHLTLRPEGTASCVRAAIQHNLVAGHGPQRLYYHGPMFRHERPQ
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCC
KGRYRQFHQIGVEALGFAGPDIDAEHIIMCARLWDDLGLEDVSLEINSLGAAEERAQHRA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHHH
ALIAYLEQHRDKLDEDGQRRLYTNPLRILDTKNPDLQPVVEAAPRLADYLGDESRAHFDA
HHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VQLFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRLGAQGTVCAGGRYDGLVAQLGGKP
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHCCCCC
QPAAGFAMGVERLLALWQESGGEPERQAPDVYVVHLGEAAQRLAFQAAEALRGHGFSAVL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEE
HCGGGSFKSQMKKADGSGASIAVVIGEDEAAAGEVGVKPLRGPGGQQRVTLAALAEAVGG
ECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
VLYSDQGDDDGGV
EEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA