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The map label for this gene is rlmN

Identifier: 119897215

GI number: 119897215

Start: 990009

End: 991136

Strand: Direct

Name: rlmN

Synonym: azo0924

Alternate gene names: 119897215

Gene position: 990009-991136 (Clockwise)

Preceding gene: 119897214

Following gene: 119897216

Centisome position: 22.62

GC content: 64.98

Gene sequence:

>1128_bases
ATGAATACCCCCGTCAATCTGCTCGATTTCGATGTCGATGGTCTCGTCGACTGGTTCGCCGGTCTGGGCGAGAAGCCCTT
CCGCGCCCGTCAGGTGATGCGCTGGATGCACCGGGAAGGCTGCGACGATTTCGACCAGATGACGGACGTGGCCAAGTCGC
TGCGGGCCAAGCTCAAGGAGATCGCGGTGATCCGGCCGCCGGTGCCGGTGCGCGATTCGGTCTCCAGCGACGGCACGCGC
AAGTGGCTGCTCGACGTCGGCAATGCAAATGCGGTGGAAACGGTGTTCATCCCCGAAACCAACCGCGGCACGCTGTGCGT
CTCCTCACAGGCCGGCTGTGCGCTCGACTGCGCGTTCTGCTCCACCGGCAAACAGGGCTTCAACCGCAACCTCACGGCGG
CGGAAATCATCGGTCAGCTGTGGCTGGCCAACAAACTGCTGGGTGCCGCGCGTGATGCCGCCGCGGATCTGGAAGCCGGC
GAAAAGGACAACGGCCGCATCATCAGCAACGTGGTGATGATGGGCATGGGCGAGCCGCTCGCCAATTTCGACAACGTCGT
CACTGCGCTGCGGCTGATGCTCGACGATCACGCCTATGGCCTGTCGCGCCGGCGGGTGACGGTGTCCACCTCCGGCATCG
TGCCGGCGATCGACCGCCTGCGCGATGAATGCCCGGTGGCGCTGGCGGTGTCGCTGCATGCCTCCAACGACGCGCTGCGC
GACCGCCTGGTGCCGATCAACCAGAAGTATCCGCTGCGCGAGCTGATGGCGGCCTGCCAGCGATATCTCGAGCGCGCGCC
GCGCGACTTCATCACCTTCGAATACGTGATGCTCGATGGCGTGAACGACCAGGAAGCGCACGCCCGCGAACTGATCGCAC
TGGTGCGCGACGTGCCCTGCAAGTTCAACCTGATCCCGTTCAATCCCTTCCCCAACTCGGGATTCCAGCGCTCGAATGCG
GAACGCATCCGGCGCTTTGCCGGTATCCTGCTCGACGCCGGCATCGTCACCACCACCCGCAAGACGCGTGGCGACGATGT
CGATGCGGCCTGTGGCCAGCTGGCTGGTCAGGTGCAGGACAAGACCCGGCGCACCGTGCGCCTCAAGCAGTCGATGGAGG
TTCGCTGA

Upstream 100 bases:

>100_bases
TTATTCCCGCTAAGCGGTCCATCCTGACGGCCCGTCGCGCCCGCTTCGCGGTAATTTCACCGCGAAAGGCGCGGGCGGGC
CGTTTGCGTTGGAGGCAAAC

Downstream 100 bases:

>100_bases
TGGCGCGTAAAGCCGCGTCCCTGCTGCTGATGATGCTCGCGCTCTCCGGGTGCGTGACGTCGACGACGCGCCCCGGCCCG
GCCGTGAGTTCCGGATCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNTPVNLLDFDVDGLVDWFAGLGEKPFRARQVMRWMHREGCDDFDQMTDVAKSLRAKLKEIAVIRPPVPVRDSVSSDGTR
KWLLDVGNANAVETVFIPETNRGTLCVSSQAGCALDCAFCSTGKQGFNRNLTAAEIIGQLWLANKLLGAARDAAADLEAG
EKDNGRIISNVVMMGMGEPLANFDNVVTALRLMLDDHAYGLSRRRVTVSTSGIVPAIDRLRDECPVALAVSLHASNDALR
DRLVPINQKYPLRELMAACQRYLERAPRDFITFEYVMLDGVNDQEAHARELIALVRDVPCKFNLIPFNPFPNSGFQRSNA
ERIRRFAGILLDAGIVTTTRKTRGDDVDAACGQLAGQVQDKTRRTVRLKQSMEVR

Sequences:

>Translated_375_residues
MNTPVNLLDFDVDGLVDWFAGLGEKPFRARQVMRWMHREGCDDFDQMTDVAKSLRAKLKEIAVIRPPVPVRDSVSSDGTR
KWLLDVGNANAVETVFIPETNRGTLCVSSQAGCALDCAFCSTGKQGFNRNLTAAEIIGQLWLANKLLGAARDAAADLEAG
EKDNGRIISNVVMMGMGEPLANFDNVVTALRLMLDDHAYGLSRRRVTVSTSGIVPAIDRLRDECPVALAVSLHASNDALR
DRLVPINQKYPLRELMAACQRYLERAPRDFITFEYVMLDGVNDQEAHARELIALVRDVPCKFNLIPFNPFPNSGFQRSNA
ERIRRFAGILLDAGIVTTTRKTRGDDVDAACGQLAGQVQDKTRRTVRLKQSMEVR
>Mature_375_residues
MNTPVNLLDFDVDGLVDWFAGLGEKPFRARQVMRWMHREGCDDFDQMTDVAKSLRAKLKEIAVIRPPVPVRDSVSSDGTR
KWLLDVGNANAVETVFIPETNRGTLCVSSQAGCALDCAFCSTGKQGFNRNLTAAEIIGQLWLANKLLGAARDAAADLEAG
EKDNGRIISNVVMMGMGEPLANFDNVVTALRLMLDDHAYGLSRRRVTVSTSGIVPAIDRLRDECPVALAVSLHASNDALR
DRLVPINQKYPLRELMAACQRYLERAPRDFITFEYVMLDGVNDQEAHARELIALVRDVPCKFNLIPFNPFPNSGFQRSNA
ERIRRFAGILLDAGIVTTTRKTRGDDVDAACGQLAGQVQDKTRRTVRLKQSMEVR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=371, Percent_Identity=56.6037735849057, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_AZOSB (A1K3Y6)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932428.1
- ProteinModelPortal:   A1K3Y6
- STRING:   A1K3Y6
- GeneID:   4609350
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0924
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1K3Y6
- ProtClustDB:   CLSK766381
- BioCyc:   ASP62928:AZO0924-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41564; Mature: 41564

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPVNLLDFDVDGLVDWFAGLGEKPFRARQVMRWMHREGCDDFDQMTDVAKSLRAKLKE
CCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IAVIRPPVPVRDSVSSDGTRKWLLDVGNANAVETVFIPETNRGTLCVSSQAGCALDCAFC
HEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEHHH
STGKQGFNRNLTAAEIIGQLWLANKLLGAARDAAADLEAGEKDNGRIISNVVMMGMGEPL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCH
ANFDNVVTALRLMLDDHAYGLSRRRVTVSTSGIVPAIDRLRDECPVALAVSLHASNDALR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHH
DRLVPINQKYPLRELMAACQRYLERAPRDFITFEYVMLDGVNDQEAHARELIALVRDVPC
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCC
KFNLIPFNPFPNSGFQRSNAERIRRFAGILLDAGIVTTTRKTRGDDVDAACGQLAGQVQD
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHH
KTRRTVRLKQSMEVR
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTPVNLLDFDVDGLVDWFAGLGEKPFRARQVMRWMHREGCDDFDQMTDVAKSLRAKLKE
CCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IAVIRPPVPVRDSVSSDGTRKWLLDVGNANAVETVFIPETNRGTLCVSSQAGCALDCAFC
HEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEHHH
STGKQGFNRNLTAAEIIGQLWLANKLLGAARDAAADLEAGEKDNGRIISNVVMMGMGEPL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCH
ANFDNVVTALRLMLDDHAYGLSRRRVTVSTSGIVPAIDRLRDECPVALAVSLHASNDALR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHH
DRLVPINQKYPLRELMAACQRYLERAPRDFITFEYVMLDGVNDQEAHARELIALVRDVPC
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCC
KFNLIPFNPFPNSGFQRSNAERIRRFAGILLDAGIVTTTRKTRGDDVDAACGQLAGQVQD
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHH
KTRRTVRLKQSMEVR
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA