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The map label for this gene is queA

Identifier: 119897199

GI number: 119897199

Start: 972316

End: 973353

Strand: Reverse

Name: queA

Synonym: azo0908

Alternate gene names: 119897199

Gene position: 973353-972316 (Counterclockwise)

Preceding gene: 119897200

Following gene: 119897198

Centisome position: 22.24

GC content: 68.79

Gene sequence:

>1038_bases
ATGACGCTTTCGCTTCAAGATTTCGACTACGACCTGCCGCCGGACCTGATCGCGCAGGCGCCGCTGGCTGAACGCAGCGC
CAGCCGCCTGCTGGTGGTGGATGGCGACGTCCTCGCCGACCGCCGCTTCGTCGACCTGCCAGACTTCATCCGGCCGGGGG
ATCTCCTCGTGTTCAACGACACGCGGGTGCTGCATGCACGCCTGTTCGGCGTCAAGGCGACCGGCGGCCAGGTCGAAGTG
CTGGTCGAGCGCCCGATCGGTGCGCACGAGGCGCTGGCCCAGATCCGCGCCAGCAAATCCCCCAAGCCCGGCAGCACGCT
GCGGCTTGCGGATGCGGTGGACGTGACCGTGCTCGGCCGCAGTGGCGAGTTCTTTCATCTGCGCTTTCCCGACGACGAGA
ACGTCGTGGACGTGCTGGAACGCTACGGGAAGCTGCCGCTGCCGCCTTACATCCAGCGCGCCGCGGGCGACGCCGACGAG
GCGCGCTACCAGACGGTGTTTGCCCGCGCGCCCGGATCGGTCGCCGCGCCCACCGCGGGGCTGCATTTCGACGACGCGGT
CCTCGACCGACTGCGCGCGCGCGGTGCCGGCTGTGCCTGGGTCACCCTGCATGTCGGCGCCGGCACCTTCCAGCCGGTGC
GCGTGGACGATCTCGCCCAGCATCGCATGCACAGCGAGCGCTACGTCATTCCGCAGGAAACCGTGGAAGCGATTGCGCGG
ACGCGGGCGAACGGCGGCCGTGTCGTCGCGGTCGGAACGACCAGCATGCGGGCGCTGGAAGCCGCCGCCCAGGCCGGCCC
GCTTGCCGCCGGCAGTGGCGAGACTGACATCTTCATCCTGCCGGGCTTCCGCTTCCGGGTGGCAGACATGCTGGTCACCA
ACTTCCACCTGCCGAAGTCCACGCTGCTGATGCTCGTGTCCGCCTTTTCCGGCACCGACGTCATCCGCCGCGCCTATGCC
CACGCGGTGGCGTCGCGCTACCGCTTCTTCAGCTATGGCGACGCCATGCTCCTCACGCGCAACGATCATGCAATTTGA

Upstream 100 bases:

>100_bases
ATTTTGAATCCGCTGCGTCTACCGATTCCGCCATCCCGGCACGGGAAGGACGCGCATTATTACTGATCCCCCTGCTGCCC
GGCAACCTGCCCTTCTTAGC

Downstream 100 bases:

>100_bases
ACTTCTCTCCACCAGCGCCGGCGCACGTCGCGGCCGCCTGACTCTGGCTCACGGAGCCGTCGAAACGCCGGTCTTCATGC
CCGTCGGCACCTACGGCACC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTLSLQDFDYDLPPDLIAQAPLAERSASRLLVVDGDVLADRRFVDLPDFIRPGDLLVFNDTRVLHARLFGVKATGGQVEV
LVERPIGAHEALAQIRASKSPKPGSTLRLADAVDVTVLGRSGEFFHLRFPDDENVVDVLERYGKLPLPPYIQRAAGDADE
ARYQTVFARAPGSVAAPTAGLHFDDAVLDRLRARGAGCAWVTLHVGAGTFQPVRVDDLAQHRMHSERYVIPQETVEAIAR
TRANGGRVVAVGTTSMRALEAAAQAGPLAAGSGETDIFILPGFRFRVADMLVTNFHLPKSTLLMLVSAFSGTDVIRRAYA
HAVASRYRFFSYGDAMLLTRNDHAI

Sequences:

>Translated_345_residues
MTLSLQDFDYDLPPDLIAQAPLAERSASRLLVVDGDVLADRRFVDLPDFIRPGDLLVFNDTRVLHARLFGVKATGGQVEV
LVERPIGAHEALAQIRASKSPKPGSTLRLADAVDVTVLGRSGEFFHLRFPDDENVVDVLERYGKLPLPPYIQRAAGDADE
ARYQTVFARAPGSVAAPTAGLHFDDAVLDRLRARGAGCAWVTLHVGAGTFQPVRVDDLAQHRMHSERYVIPQETVEAIAR
TRANGGRVVAVGTTSMRALEAAAQAGPLAAGSGETDIFILPGFRFRVADMLVTNFHLPKSTLLMLVSAFSGTDVIRRAYA
HAVASRYRFFSYGDAMLLTRNDHAI
>Mature_344_residues
TLSLQDFDYDLPPDLIAQAPLAERSASRLLVVDGDVLADRRFVDLPDFIRPGDLLVFNDTRVLHARLFGVKATGGQVEVL
VERPIGAHEALAQIRASKSPKPGSTLRLADAVDVTVLGRSGEFFHLRFPDDENVVDVLERYGKLPLPPYIQRAAGDADEA
RYQTVFARAPGSVAAPTAGLHFDDAVLDRLRARGAGCAWVTLHVGAGTFQPVRVDDLAQHRMHSERYVIPQETVEAIART
RANGGRVVAVGTTSMRALEAAAQAGPLAAGSGETDIFILPGFRFRVADMLVTNFHLPKSTLLMLVSAFSGTDVIRRAYAH
AVASRYRFFSYGDAMLLTRNDHAI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=54.5714285714286, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_AZOSB (A1K3X0)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932412.1
- ProteinModelPortal:   A1K3X0
- SMR:   A1K3X0
- STRING:   A1K3X0
- GeneID:   4609932
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0908
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   A1K3X0
- ProtClustDB:   PRK00147
- BioCyc:   ASP62928:AZO0908-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37554; Mature: 37423

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSLQDFDYDLPPDLIAQAPLAERSASRLLVVDGDVLADRRFVDLPDFIRPGDLLVFND
CEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCEECCCEEECCCCCCCCCCEEEECC
TRVLHARLFGVKATGGQVEVLVERPIGAHEALAQIRASKSPKPGSTLRLADAVDVTVLGR
CEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECC
SGEFFHLRFPDDENVVDVLERYGKLPLPPYIQRAAGDADEARYQTVFARAPGSVAAPTAG
CCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHEEEEEEECCCCCCCCCCC
LHFDDAVLDRLRARGAGCAWVTLHVGAGTFQPVRVDDLAQHRMHSERYVIPQETVEAIAR
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEECCHHHHHHHHH
TRANGGRVVAVGTTSMRALEAAAQAGPLAAGSGETDIFILPGFRFRVADMLVTNFHLPKS
HCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHH
TLLMLVSAFSGTDVIRRAYAHAVASRYRFFSYGDAMLLTRNDHAI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCC
>Mature Secondary Structure 
TLSLQDFDYDLPPDLIAQAPLAERSASRLLVVDGDVLADRRFVDLPDFIRPGDLLVFND
EEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCEECCCEEECCCCCCCCCCEEEECC
TRVLHARLFGVKATGGQVEVLVERPIGAHEALAQIRASKSPKPGSTLRLADAVDVTVLGR
CEEEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECC
SGEFFHLRFPDDENVVDVLERYGKLPLPPYIQRAAGDADEARYQTVFARAPGSVAAPTAG
CCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHEEEEEEECCCCCCCCCCC
LHFDDAVLDRLRARGAGCAWVTLHVGAGTFQPVRVDDLAQHRMHSERYVIPQETVEAIAR
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEECCHHHHHHHHH
TRANGGRVVAVGTTSMRALEAAAQAGPLAAGSGETDIFILPGFRFRVADMLVTNFHLPKS
HCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHH
TLLMLVSAFSGTDVIRRAYAHAVASRYRFFSYGDAMLLTRNDHAI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA