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The map label for this gene is tgt

Identifier: 119897198

GI number: 119897198

Start: 971214

End: 972326

Strand: Reverse

Name: tgt

Synonym: azo0907

Alternate gene names: 119897198

Gene position: 972326-971214 (Counterclockwise)

Preceding gene: 119897199

Following gene: 119897196

Centisome position: 22.22

GC content: 66.22

Gene sequence:

>1113_bases
ATGCAATTTGAACTTCTCTCCACCAGCGCCGGCGCACGTCGCGGCCGCCTGACTCTGGCTCACGGAGCCGTCGAAACGCC
GGTCTTCATGCCCGTCGGCACCTACGGCACCGTCAAGGCGATGACGCCCGCAATGCTGAGCGACGTCGGCGCGCAGATCT
GTCTGGGCAACACCTTCCACCTCTGGCTGCGCCCCGGGCTCGACATCGTGGGCGCCCACGGCGGCCTGCACCGCTTCATG
GGCTGGGACAAGCCCATCCTCACCGACTCCGGCGGCTTTCAGGTGTTCAGCCTCGGGGCGCTGCGCAAGATCAGCGAGGA
AGGCGTCAAATTTGCCAGCCCGATCGACGGCGCCAGGCTCTTCCTGACGCCCGAAATCTCGATGCAGATCCAGACCGTGC
TGAACTCGGACGTGGTCATGATCTTCGACGAATGCACGCCCTACCCCGCCACCCGCGACGAAGCAGCGAAGTCGATGCGG
CTGTCGCGACGCTGGGCACGGCGCTCGCGCGACGAGTTCGACCGGCTGGAAAACGCGAACGCGCTGTTCGGTATCGTGCA
GGGCGGCATGTACGAGGACCTGCGCGACGAATCGCTCGGCGCGCTGCAGGACATCGGCTTTCACGGCTTCGCCATCGGCG
GACTGTCGGTCGGCGAACCGAAGGACGACATGGCCCGCATCCTCGCCCACACCGCACCGCGACTGCCCGCCGACAAGCCG
CGCTACCTGATGGGCGTCGGCACGCCCGAAGACATCGTCGACGGCATCGCCAACGGGATCGACATGTTCGACTGCGTGAT
GCCCACGCGCAACGCACGCAACGGCTGGCTGTTTACCCGCTACGGCGACCTCAAGATCAAGAACGCGGTCCACAAGGCGG
ATACCCGGCCGCTCGACCCGTCCTGCAGTTGCTACACCTGCCGCAACTTCAGCCGCAGCTACCTGCACCACCTGCACCGC
GCGGGCGAAATCCTCGGCAGCATGCTCAACACGGTGCACAACCTGCACTACTACCAGACGCTCACCGCGGAACTGCGCGA
TGCGATCGCCGCGGACCGCTTCGCCGACTACGTCACCCGTTTCCGCAGCGAGCGCGCCACCGGAGCGCACTGA

Upstream 100 bases:

>100_bases
TCCGGCACCGACGTCATCCGCCGCGCCTATGCCCACGCGGTGGCGTCGCGCTACCGCTTCTTCAGCTATGGCGACGCCAT
GCTCCTCACGCGCAACGATC

Downstream 100 bases:

>100_bases
GTCCGCCCGGCGTCACGCGCCGGCGATCTCGAAGCTGTCGCGCACGTCGGCCAGATAATCGGCCAGTTCGTCCGGGTGGA
TGCCGAGTTCGCGCACCACC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQFELLSTSAGARRGRLTLAHGAVETPVFMPVGTYGTVKAMTPAMLSDVGAQICLGNTFHLWLRPGLDIVGAHGGLHRFM
GWDKPILTDSGGFQVFSLGALRKISEEGVKFASPIDGARLFLTPEISMQIQTVLNSDVVMIFDECTPYPATRDEAAKSMR
LSRRWARRSRDEFDRLENANALFGIVQGGMYEDLRDESLGALQDIGFHGFAIGGLSVGEPKDDMARILAHTAPRLPADKP
RYLMGVGTPEDIVDGIANGIDMFDCVMPTRNARNGWLFTRYGDLKIKNAVHKADTRPLDPSCSCYTCRNFSRSYLHHLHR
AGEILGSMLNTVHNLHYYQTLTAELRDAIAADRFADYVTRFRSERATGAH

Sequences:

>Translated_370_residues
MQFELLSTSAGARRGRLTLAHGAVETPVFMPVGTYGTVKAMTPAMLSDVGAQICLGNTFHLWLRPGLDIVGAHGGLHRFM
GWDKPILTDSGGFQVFSLGALRKISEEGVKFASPIDGARLFLTPEISMQIQTVLNSDVVMIFDECTPYPATRDEAAKSMR
LSRRWARRSRDEFDRLENANALFGIVQGGMYEDLRDESLGALQDIGFHGFAIGGLSVGEPKDDMARILAHTAPRLPADKP
RYLMGVGTPEDIVDGIANGIDMFDCVMPTRNARNGWLFTRYGDLKIKNAVHKADTRPLDPSCSCYTCRNFSRSYLHHLHR
AGEILGSMLNTVHNLHYYQTLTAELRDAIAADRFADYVTRFRSERATGAH
>Mature_370_residues
MQFELLSTSAGARRGRLTLAHGAVETPVFMPVGTYGTVKAMTPAMLSDVGAQICLGNTFHLWLRPGLDIVGAHGGLHRFM
GWDKPILTDSGGFQVFSLGALRKISEEGVKFASPIDGARLFLTPEISMQIQTVLNSDVVMIFDECTPYPATRDEAAKSMR
LSRRWARRSRDEFDRLENANALFGIVQGGMYEDLRDESLGALQDIGFHGFAIGGLSVGEPKDDMARILAHTAPRLPADKP
RYLMGVGTPEDIVDGIANGIDMFDCVMPTRNARNGWLFTRYGDLKIKNAVHKADTRPLDPSCSCYTCRNFSRSYLHHLHR
AGEILGSMLNTVHNLHYYQTLTAELRDAIAADRFADYVTRFRSERATGAH

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=46, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=66.2049861495845, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.9889502762431, Blast_Score=280, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=162, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=352, Percent_Identity=45.7386363636364, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI21355311, Length=180, Percent_Identity=30.5555555555556, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_AZOSB (A1K3W9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932411.1
- ProteinModelPortal:   A1K3W9
- SMR:   A1K3W9
- STRING:   A1K3W9
- GeneID:   4606629
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0907
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   A1K3W9
- ProtClustDB:   PRK00112
- BioCyc:   ASP62928:AZO0907-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 40941; Mature: 40941

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFELLSTSAGARRGRLTLAHGAVETPVFMPVGTYGTVKAMTPAMLSDVGAQICLGNTFH
CCEEEECCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCEEE
LWLRPGLDIVGAHGGLHRFMGWDKPILTDSGGFQVFSLGALRKISEEGVKFASPIDGARL
EEEECCCEEEECCCCHHHHCCCCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCCCEE
FLTPEISMQIQTVLNSDVVMIFDECTPYPATRDEAAKSMRLSRRWARRSRDEFDRLENAN
EEECCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
ALFGIVQGGMYEDLRDESLGALQDIGFHGFAIGGLSVGEPKDDMARILAHTAPRLPADKP
CEEHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGTPEDIVDGIANGIDMFDCVMPTRNARNGWLFTRYGDLKIKNAVHKADTRPLDP
CEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHCCCCCCCC
SCSCYTCRNFSRSYLHHLHRAGEILGSMLNTVHNLHYYQTLTAELRDAIAADRFADYVTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRSERATGAH
HHHCCCCCCC
>Mature Secondary Structure
MQFELLSTSAGARRGRLTLAHGAVETPVFMPVGTYGTVKAMTPAMLSDVGAQICLGNTFH
CCEEEECCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCEEE
LWLRPGLDIVGAHGGLHRFMGWDKPILTDSGGFQVFSLGALRKISEEGVKFASPIDGARL
EEEECCCEEEECCCCHHHHCCCCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCCCEE
FLTPEISMQIQTVLNSDVVMIFDECTPYPATRDEAAKSMRLSRRWARRSRDEFDRLENAN
EEECCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
ALFGIVQGGMYEDLRDESLGALQDIGFHGFAIGGLSVGEPKDDMARILAHTAPRLPADKP
CEEHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGTPEDIVDGIANGIDMFDCVMPTRNARNGWLFTRYGDLKIKNAVHKADTRPLDP
CEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHCCCCCCCC
SCSCYTCRNFSRSYLHHLHRAGEILGSMLNTVHNLHYYQTLTAELRDAIAADRFADYVTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRSERATGAH
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA