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The map label for this gene is murD [H]

Identifier: 119897173

GI number: 119897173

Start: 943386

End: 944804

Strand: Direct

Name: murD [H]

Synonym: azo0882

Alternate gene names: 119897173

Gene position: 943386-944804 (Clockwise)

Preceding gene: 119897172

Following gene: 119897174

Centisome position: 21.56

GC content: 72.87

Gene sequence:

>1419_bases
ATGACGCAGCCGTCCTTCTCCTCCTCGCTTGCTGGCCGCCACGTGCTCGTGCTCGGGCTGGGCGAGTCCGGTCTGGCCAT
CGCCCGCTGGTGCGCGCGCTGCGGCGCGCGTCTGCGCGTGGCCGACAGCCGCACGACGCCGCCGGGGCTGGAGGCGCTGC
GCGCTGCCGCGCCGCAGGCCGAAGTCATCACCGGCAGCTTCGGTGACGAAGTGCTGGAAGGAATCGACGTGGTGGCAGTC
AGCCCGGGGCTGGACCCGCGGGTGGGCGTGATCGCGAGCGCGCGCCGCCGCTGCCTGCCGATCACCGGCGAGATGGCGCT
GTTCGCCCAGGCCCTCACTGATCTCGGCGCGCGCGATGCGACCCGCATCCTCGCGATCACCGGCACCAACGGCAAGACCA
CCACCACCGCGCTGACCGCCGCGCTCGCGCAGTCGGCCGGGCTGGATGCGGTGGCGGCGGGCAATATCAGCCCGGCCGCG
CTCGATGTGCTGATGGCGCGACTCGACGCCGGTCAGGCGCTGCCGCAATGCTGGGTGCTGGAGCTTTCCAGCTTCCAGAT
CGAGACCGCGCAGGCGCTCGACGCCGATGCCGCGACGGTGCTCAACGTCACCGACGACCATCTCGACCGCTACGCCGATC
TCGCCGATTACGCCGCCACCAAGGCGCGCATCTTCCAGGGGCGTGGAGCGCAGGTGCTCAACCGCGAAGACGCGCGGGTC
GCCGCCATGGTGCTGTCCGGCCGCAAGGTCGTGCGCTTCGGCACCGATGCACCGCAGAATCCGGCCGATTACGGCCTGGT
CGAAGACGCCGGGCGCTGCTGGCTGGTGCGCGGCGGCGAACGGCTGCTCGCGCTCGACGAACTCGCGCTCGCCGGCCGCC
ACAACGCCGCCAACGCGCTCGCCGCGCTGGCGCTGTGCGAAACCGGCCTCGGCCTCGCGCCGGCGCAGTTGCTCGCCGGT
CTGCGTGCCTTCCGCGGCCTGCCGCACCGGGTGGAGCTGGTGGCCGAGCGCGCCGACGGCGTGCGCTACTACGACGACTC
CAAGGGCACCAACGTCGGCGCCACCGTGGCTGCGCTCGACGGACTCGGCGGTCGCGTGGTGCTGATCGCCGGCGGCGATG
GCAAGGGCCAGGACTTTTCGCCGCTCGCGCCGGTGCTCACGCGCCATGCGCGCGCGGTGGTGTTGATCGGCCGCGACGCG
AAGCTGATCGAAGCCGCCGTGGCCGGATGCGGCGTGCCGCTGGAACACGCCGCCGACCTCGACACCGCGGTGCTGCGCGC
CAATGCGCTGGCGCGGCCGGGTGACGCAGTGATGCTGTCCCCCGCGTGCGCCAGCCTCGACATGTTCCGCAACTACGCCC
ACCGCGCCGAAGTGTTCATCGCCGCGGTGCGCCGCCTGCCCGAGGTCAGCCCGCGATGA

Upstream 100 bases:

>100_bases
ACTACGAACTGAGCGGCTGGAAGGAAACCCAGGTGGTGGTGCGCTTCTGGATCATCACCCTGATGCTGGTGTTGTTCGGC
CTGTCGACGCTGAAACTGAG

Downstream 100 bases:

>100_bases
AGCTCGGCGCCCTGTTCTCGGCCTGGTTCCCGTCGCGGCCGGCGCCTGTCGGCGGCGGCGAGACGGCGCGCGTGGTCAGC
CGCCTTGCGCGGCCGGGCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTQPSFSSSLAGRHVLVLGLGESGLAIARWCARCGARLRVADSRTTPPGLEALRAAAPQAEVITGSFGDEVLEGIDVVAV
SPGLDPRVGVIASARRRCLPITGEMALFAQALTDLGARDATRILAITGTNGKTTTTALTAALAQSAGLDAVAAGNISPAA
LDVLMARLDAGQALPQCWVLELSSFQIETAQALDADAATVLNVTDDHLDRYADLADYAATKARIFQGRGAQVLNREDARV
AAMVLSGRKVVRFGTDAPQNPADYGLVEDAGRCWLVRGGERLLALDELALAGRHNAANALAALALCETGLGLAPAQLLAG
LRAFRGLPHRVELVAERADGVRYYDDSKGTNVGATVAALDGLGGRVVLIAGGDGKGQDFSPLAPVLTRHARAVVLIGRDA
KLIEAAVAGCGVPLEHAADLDTAVLRANALARPGDAVMLSPACASLDMFRNYAHRAEVFIAAVRRLPEVSPR

Sequences:

>Translated_472_residues
MTQPSFSSSLAGRHVLVLGLGESGLAIARWCARCGARLRVADSRTTPPGLEALRAAAPQAEVITGSFGDEVLEGIDVVAV
SPGLDPRVGVIASARRRCLPITGEMALFAQALTDLGARDATRILAITGTNGKTTTTALTAALAQSAGLDAVAAGNISPAA
LDVLMARLDAGQALPQCWVLELSSFQIETAQALDADAATVLNVTDDHLDRYADLADYAATKARIFQGRGAQVLNREDARV
AAMVLSGRKVVRFGTDAPQNPADYGLVEDAGRCWLVRGGERLLALDELALAGRHNAANALAALALCETGLGLAPAQLLAG
LRAFRGLPHRVELVAERADGVRYYDDSKGTNVGATVAALDGLGGRVVLIAGGDGKGQDFSPLAPVLTRHARAVVLIGRDA
KLIEAAVAGCGVPLEHAADLDTAVLRANALARPGDAVMLSPACASLDMFRNYAHRAEVFIAAVRRLPEVSPR
>Mature_471_residues
TQPSFSSSLAGRHVLVLGLGESGLAIARWCARCGARLRVADSRTTPPGLEALRAAAPQAEVITGSFGDEVLEGIDVVAVS
PGLDPRVGVIASARRRCLPITGEMALFAQALTDLGARDATRILAITGTNGKTTTTALTAALAQSAGLDAVAAGNISPAAL
DVLMARLDAGQALPQCWVLELSSFQIETAQALDADAATVLNVTDDHLDRYADLADYAATKARIFQGRGAQVLNREDARVA
AMVLSGRKVVRFGTDAPQNPADYGLVEDAGRCWLVRGGERLLALDELALAGRHNAANALAALALCETGLGLAPAQLLAGL
RAFRGLPHRVELVAERADGVRYYDDSKGTNVGATVAALDGLGGRVVLIAGGDGKGQDFSPLAPVLTRHARAVVLIGRDAK
LIEAAVAGCGVPLEHAADLDTAVLRANALARPGDAVMLSPACASLDMFRNYAHRAEVFIAAVRRLPEVSPR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=460, Percent_Identity=40.4347826086956, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48986; Mature: 48854

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPSFSSSLAGRHVLVLGLGESGLAIARWCARCGARLRVADSRTTPPGLEALRAAAPQA
CCCCCCCHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCC
EVITGSFGDEVLEGIDVVAVSPGLDPRVGVIASARRRCLPITGEMALFAQALTDLGARDA
EEEECCHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
TRILAITGTNGKTTTTALTAALAQSAGLDAVAAGNISPAALDVLMARLDAGQALPQCWVL
EEEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEE
ELSSFQIETAQALDADAATVLNVTDDHLDRYADLADYAATKARIFQGRGAQVLNREDARV
ECCCCEEEHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHE
AAMVLSGRKVVRFGTDAPQNPADYGLVEDAGRCWLVRGGERLLALDELALAGRHNAANAL
EEHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCCHHHHH
AALALCETGLGLAPAQLLAGLRAFRGLPHRVELVAERADGVRYYDDSKGTNVGATVAALD
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHEECC
GLGGRVVLIAGGDGKGQDFSPLAPVLTRHARAVVLIGRDAKLIEAAVAGCGVPLEHAADL
CCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCH
DTAVLRANALARPGDAVMLSPACASLDMFRNYAHRAEVFIAAVRRLPEVSPR
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQPSFSSSLAGRHVLVLGLGESGLAIARWCARCGARLRVADSRTTPPGLEALRAAAPQA
CCCCCCHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCC
EVITGSFGDEVLEGIDVVAVSPGLDPRVGVIASARRRCLPITGEMALFAQALTDLGARDA
EEEECCHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
TRILAITGTNGKTTTTALTAALAQSAGLDAVAAGNISPAALDVLMARLDAGQALPQCWVL
EEEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEE
ELSSFQIETAQALDADAATVLNVTDDHLDRYADLADYAATKARIFQGRGAQVLNREDARV
ECCCCEEEHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHE
AAMVLSGRKVVRFGTDAPQNPADYGLVEDAGRCWLVRGGERLLALDELALAGRHNAANAL
EEHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCCHHHHH
AALALCETGLGLAPAQLLAGLRAFRGLPHRVELVAERADGVRYYDDSKGTNVGATVAALD
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHEECC
GLGGRVVLIAGGDGKGQDFSPLAPVLTRHARAVVLIGRDAKLIEAAVAGCGVPLEHAADL
CCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCH
DTAVLRANALARPGDAVMLSPACASLDMFRNYAHRAEVFIAAVRRLPEVSPR
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA